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Citations for Addgene :
8601 - 8650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: We used AAV1-hSyn-GCaMP6f (2 × 1013 vg/mL Penn Vector Core/Addgene; diluted 1:8 to 1:15 in saline) for experiments involving two-photon calcium imaging of V1 layer 2/3 cells ...
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bioRxiv - Neuroscience 2023Quote: ... UC San Diego) or AAV5-CAG-dLight1.1 (8.1 x 1012 GC/ml, RRID:Addgene_111067) were similarly infused unilaterally into the VP ...
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bioRxiv - Neuroscience 2023Quote: ... AAVDJ-EF1α -fDIO-hChR2-EYFP (2.19 x 1013 GC/ml, plasmid RRID:Addgene_55639, Vigene production) or AAV5-EF1α-DIO-EYFP (6.5 x 1012 GC/ml ...
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bioRxiv - Neuroscience 2023Quote: ... and 150-250 nL of AAV5-EF1α-DIO-hChR2(H134R)-mCherry (5.25 x 1012 GC/ml, RRID:Addgene_20297), AAV5-EF1α-DIO-mCherry (7.3 x 1012 GC/ml ...
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bioRxiv - Neuroscience 2023Quote: We performed an initial calibrating analysis on Emx-Cre mice (see Animals) expressing Cre-dependent GFP virus (Addgene pCAG-FLEX-EGFP-WPRE; #51502-AAV9) following a dot-wise application of 1:2000 diluted virus (see Organotypic Slice Culture methods) ...
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bioRxiv - Neuroscience 2023Quote: ... We injected AAV5.EF1.dflox.hChR2(H134R)- mCherry.WPRE.hGH (based on Addgene plasmid #20297, UNC Vector Core) cre- dependent virus into the frontal cortex ...
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bioRxiv - Neuroscience 2023Quote: We obtained pAAV-Ef1a-FLPo (Addgene plasmid #55637 ...
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bioRxiv - Molecular Biology 2023Quote: ... pUGI-NLS UDG Inhibitor (UGI) plasmid was purchased from Addgene (Cat#101091).
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bioRxiv - Neuroscience 2023Quote: ... virus or AAV2- FLEX-sun1GFP (Addgene plasmid# 160141) control virus were packaged by SignaGen at high-titer (1 × 103 gc/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... and the second-generation packaging plasmid psPAX2 (Addgene #12260) into Hek293T cells (ATCC ...
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bioRxiv - Molecular Biology 2023Quote: ... “HCT116 Cas9” or “K562 Cas9”) were generated by lentiviral transduction of the lenti.Cas9.BFP.Blast plasmid (Addgene #196714) and selected with 5 μg/ml blasticidin (Invivogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and pCMV-VSV-G (a gift from Bob Weinberg, Addgene #8454) in HEK-293T (ATCC) ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid library was packaged into the lentivirus utilising plasmids psPAX2 (a gift from Didier Trono, Addgene #12260) and pCMV-VSV-G (a gift from Bob Weinberg ...
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bioRxiv - Molecular Biology 2023Quote: This construct was then cloned into the pLenti-Puro-AU-flip-3xBsmBI (Addgene #196709) (75 ...
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bioRxiv - Neuroscience 2023Quote: ... University of Zurich (purified by VVF as v723-9; AAV9-hCMV-HA-SpCas9, Addgene 106431, gift from Juan Belmonte [22]).
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bioRxiv - Neuroscience 2023Quote: ... the p415 TEF roGFP2-Tsa2ΔCR plasmid (Addgene #83238)21 was digested by NotI and XbaI ...
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bioRxiv - Neuroscience 2023Quote: ... a 1 μL dot of AAV9/hSyn/GCaMP6f virus (Addgene, Watertown, MA) diluted 1-5x from commercial titer (2.8x1013 to ∼1x1012 units/mL ...
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bioRxiv - Microbiology 2023Quote: ... Feng Zhang (Addgene, Watertown, MA, USA, plasmid #62988; http://n2t.net/addgene:62988; RRID: Addgene_62988). pGP plasmid and pDON-5 Neo-ZsGreen plasmid were described previously [24].
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bioRxiv - Microbiology 2023Quote: ... single guide RNAs (sgRNAs) were ligated into an empty pLentiCRISPR plasmid backbone (gifted by Dr Feng Zhang; Addgene plasmid #52961 (46)) and packaged into lentivirus using XtremeGENE 9 DNA Transfection Reagent ...
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bioRxiv - Microbiology 2023Quote: ... using pBacT7-S1T1L (Addgene) for the S1 segment.
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bioRxiv - Microbiology 2023Quote: ... Cas9 (pU6_CBh-Cas9-T2A-BFP: Addgene #64323) and gRNA cassette (pSPgRNA ...
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bioRxiv - Microbiology 2023Quote: ... The luxCDABE cassette was amplified from the pAKgfplux1 plasmid (Addgene # 14083) by PCR ...
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bioRxiv - Microbiology 2023Quote: ... The human ANP32A 1-149 construct was a gift from Cynthia Wolberger (Addgene plasmid # 67241 ...
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bioRxiv - Microbiology 2023Quote: Plasmids used in generating recombinant SA11 rotaviruses were obtained from Addgene [https://www.addgene.org/Takeshi_Kobayashi/] and included pT7/VP1SA11 ...
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bioRxiv - Microbiology 2023Quote: ... which was a gift from Tobias Spielmann (Addgene plasmid #85793 ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA encoding human cGAS (NM_138441.3) was purchased from Addgene (#108674) and subcloned into pEGFP-C3 vector.
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bioRxiv - Microbiology 2023Quote: ... lentiCRISPR v2 was a gift from Feng Zhang (Addgene plasmid# 52961; http://n2t.net/addgene:52961; RRID: Addgene_52961)63 ...
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bioRxiv - Microbiology 2023Quote: ... Pantelis Tsoulfas (36083; http://n2t.net/addgene:36 083; RRID: Addgene_36083)64 ...
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bioRxiv - Microbiology 2023Quote: ... pMD2.G (12259; http://n2t.net/addgene:12259; RRID: Addgene_12259) and psPAX2 (12260 ...
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bioRxiv - Microbiology 2023Quote: ... pLionII (1730; http://n2t.net/addgene:1730; RRID: Addgene_1730) and pCPRDEnv (1732 ...
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bioRxiv - Microbiology 2023Quote: ... and pEV53D (44168; http://n2t.net/addgene:44168; RRID: Addgene_44168) were gifts from Dr ...
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bioRxiv - Microbiology 2023Quote: ... (kind gift from Jan-Willem Veening) (Addgene plasmid # 122633) (49).
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bioRxiv - Neuroscience 2023Quote: ... AAV2/1.syn.jGCaMP7s 50 (Addgene, 104487-AAV1 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were co-transfected with 250ng of 3X ERE with TATA-box luciferase plasmid (Addgene 11354), a gift from Donald McDonnell ...
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bioRxiv - Molecular Biology 2023Quote: ... iLID-CAAX (Addgene plasmid # 85680), melanopsin ...
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bioRxiv - Molecular Biology 2023Quote: Firefly and Renilla luciferase expression plasmids were cloned by standard methods starting with the pGL3 PUb-luc plasmid described previously (7) and pSLfa-PUb-MCS (Addgene plasmid # 52908). Transgenesis plasmids were generated using NEBuilder HiFi Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2023Quote: pMBP-MS2 (Plasmid #65104, Addgene) was transformed into BLE1 DE3 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... pBabe-Puro-IKBalpha-wt (Addgene plasmid #15290), dCas9-p65 ...
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bioRxiv - Neuroscience 2023Quote: ... we employed Vgat-ZI- ChR2 mice in which we injected AAV5-EF1a-DIO-hChR2(H134R)-eYFP (UPenn Vector Core or Addgene, titers ...
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bioRxiv - Neuroscience 2023Quote: ... we injected AAV8-hSyn-EGFP (Addgene, titers ...
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bioRxiv - Molecular Biology 2023Quote: ... Injection cocktails contained 10 µg pCMV(CAT)T7-SB100 (gift from Zsuzsanna Izsvak; Addgene # 34879); 25 µg pSBbi-Myr-Akt-HA and 25 µg pSBbi-eGFP-IRES-V5-YAP5SA/ pSBbi-cJUN-WT-IRES-V5-YAP5SA/ pSBbi-cJUN-M14-IRES-V5-YAP5SA.
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bioRxiv - Molecular Biology 2023Quote: ... dCas9-VP64 and MCP-p65-HSF1 (gift from Feng Zhang, Addgene #61425 and #61426), were infected with the lentiviral SAM sgRNA library with a low titer of MOI=0.5 ...
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bioRxiv - Molecular Biology 2023Quote: The genome-wide human CRISPR/Cas9 Synergistic Activation Mediator (SAM) sgRNA library (gift from Feng Zhang, Addgene #1000000057) was amplified as recommended ...
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bioRxiv - Molecular Biology 2023Quote: Plasmids pSpCas9(BB)-2A-Puro (PX459) and pCMV-VSVG plasmid (pMD2.G) were kind gifts from Feng Zhang15 (Addgene plasmid #62988 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Didier Trono (Addgene plasmid #12259 ...
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bioRxiv - Molecular Biology 2023Quote: ... and pCMV-VSVG plasmid (pMD2.G) were kind gifts from Feng Zhang15 (Addgene plasmid #62988; https://www.addgene.org/62988/; RRID: Addgene_62988) and Didier Trono (Addgene plasmid #12259 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Didier Trono (Addgene plasmid #12259; https://www.addgene.org/12259/; RRID: Addgene_12259), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... they were Gibson assembled into the pLenti-Rfx-guide-puro backbone digested with BsmBI (Addgene #138150). Library representation in the plasmid pool was confirmed by amplifying the gRNA cassette from the plasmid pool DNA and Illumina sequencing of the PCR product (Genewiz EZ AmpliSeq).
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bioRxiv - Molecular Biology 2023Quote: Oligonucleotides corresponding to individual gRNAs were synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites ...
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bioRxiv - Neuroscience 2023Quote: ... AAVretro-CAG-FLPo and AAV-CAG-FRT-rev-TdTomato were gifts from Janelia Viral Tools (Addgene plasmid #183412, http://n2t.net/addgene:183412, RRID:Addgene_183412 ...