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Citations for Addgene :
8401 - 8450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: pDONR223_CDKN1A_WT was a gift from Jesse Boehm & William Hahn & David Root (Addgene plasmid # 82201) was cloned into pInducer20 (a gift from Stephen Elledge ...
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bioRxiv - Cancer Biology 2023Quote: ... was cloned into pInducer20 (a gift from Stephen Elledge, Addgene plasmid # 44012) by using the Gateway LR Clonase II Enzyme Mix ...
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bioRxiv - Cancer Biology 2023Quote: ... These plasmids were gifts from Bernard Futscher (Addgene plasmid # 22945, # 22934 and # 22933). tFucci(CA)5 was a gift from Atsushi Miyawaki (Addgene plasmid # 153521).
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bioRxiv - Cancer Biology 2023Quote: ... To generate H2B-Cerulean, mCerulean (Rizzo et al., 2004) was amplified from Cerulean77 (a gift from Dave Piston; Addgene #15214) by PCR and the product cut with KpnI ...
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bioRxiv - Cancer Biology 2023Quote: ... pH2B-GFP78 (a gift from Geoff Wahl; Addgene #11680) was cut with NotI ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were plated in 6-well plate and co-transfected with SBE4-Luc plasmid (1 μg, Addgene,16495) and pDEST26-Renilla plasmid (0.1 μg ...
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bioRxiv - Cancer Biology 2023Quote: ... obtained from Sigma were cloned into the pSpCas9(BB)-2A-GFP bacterial plasmid sourced from Addgene (PX458; RRID: Addgene_48138) following the protocol as defined by the Zhang lab [19] and sequenced for correct oligonucleotide insertion ...
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bioRxiv - Cancer Biology 2023Quote: ... obtained from Sigma were cloned into the pSpCas9(BB)-2A-GFP bacterial plasmid sourced from Addgene (PX458; RRID: Addgene_48138) following the protocol as defined by the Zhang lab [19] and sequenced for correct oligonucleotide insertion ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.8 μg of pVSVG (Addgene, #36399) and 0.8 μg of the lentiviral plasmid hosting the cDNA of GFP lacking the first ATG (CTR ...
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bioRxiv - Cancer Biology 2023Quote: ... (ii) HEK293T with 1.3 μg of psPAX2 (Addgene, #12260), 0.8 μg of pVSVG (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... Paul Thomas (Addgene plasmid #100900, RRID:Addgene_100900). The FKBP-WRN donor plasmid construct and WRN targeting vector were verified using Sanger sequencing (ACGT DNA Sequencing Services).
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bioRxiv - Cancer Biology 2023Quote: Sequences for sgRNAs of interest were cloned into LRG2.1-GFP (Addgene #108098) (Tarumoto et al ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNAs were subcloned into the pMSCV-IRES-green fluorescence protein vector (Addgene #20672) with reference to a report by Masubuchi et al.43 The constructed plasmid was validated by sequencing prior to use.
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bioRxiv - Cancer Biology 2023Quote: CRISPR–Cas9 screen was performed using the whole genome human Brunello CRISPR knockout pooled library (Addgene #73178) (PMID ...
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bioRxiv - Cancer Biology 2023Quote: ... and pPAX2 1.5ug (Addgene, #12260), using 100uL of serum free DMEM media (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... pMDLg/pRRE 1.5ug (Addgene, #12251), and pMD2.G 0.5ug (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... the double-stranded oligonucleotide sgRNAs for human FOXM1 sequences were ligated into the BbsI sites of the FgH1tUTG plasmid (Addgene, #70183). sgRNA target sequences were listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviruses and retroviruses were produced by co-transfecting the transfer plasmids with pMD2.G (Addgene #12259, a gift from Didier Trono) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... MSCV MYC T58A puro (Addgene #18773, a gift from Scott Lowe)66 for expressing the mutant MYC ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection of third generation lentiviral vectors included 2ug of lentiviral vectors FgH1tUTG and FUCas9Cherry (Addgene, #70182), packaging vectors pRSV-Rev 1ug (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral cloning vector pLKO.1-TRC (Addgene, #10878) was used for shRNA expression ...
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bioRxiv - Cancer Biology 2023Quote: ... packaging vectors pMD2.G 0.5ug (Addgene, #12259) and pPAX2 1.5ug (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... Lenti-dCas9-KRAB-blast (Addgene #89567, a gift from Gary Hon)64 for constitutively expressing dCas9-KRAB fusion protein ...
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bioRxiv - Cancer Biology 2023Quote: ... and pMD2.G 0.5ug (Addgene, #12259) using 100uL of serum free DMEM media (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... packaging vectors pRSV-Rev 1ug (Addgene, #12253), pMDLg/pRRE 1.5ug (Addgene ...
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bioRxiv - Developmental Biology 2023Quote: ... TIR-1 was amplified from plasmid pLZ31 (Zhang et al., 2015) (Addgene #71720) and cloned under control of the hlh-3 promoter in pSL780 (Bone et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were transfected with plasmids encoding hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP (Addgene plasmid #40640) or pcDNA3.1(- ...
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bioRxiv - Developmental Biology 2023Quote: ... three different TSC isolations (at P3-P5) were transfected with hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP (Addgene plasmid #40640; (Zhao et al., 2012)) ...
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bioRxiv - Cancer Biology 2023Quote: ... pHAGE-AKT1-E17K was a gift from Gordon Mills & Kenneth Scott (Addgene plasmid # 116103).
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bioRxiv - Cell Biology 2023Quote: ... and pTriEx-mCherry-Zdk1 (Addgene plasmid # 81057) were a gift from Klaus Hahn32 ...
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bioRxiv - Cell Biology 2023Quote: ... with either pEGFP_TRPM8 (#64879, Addgene, Watertown, MA) or pEGFP-C1 (Clonthech Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: Cells were transfected with the plasmid pcDNA3_erGAP2 (a gift from Teresa Alonso and Javier García-Sancho, Addgene #78120), which encodes a low affinity fluorescent calcium biosensor ...
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bioRxiv - Cancer Biology 2023Quote: ... The lines were generated by transduction of tet-inducible lentiviral vector for ORF expression (Addgene, pInducer 20, catalog # 44012). The ADA gene was cloned into the overexpression cell line and the 11th beta-strand of GFP was used as a doxycycline control (Dox-control).
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bioRxiv - Cancer Biology 2023Quote: The Human Brunello v2 CRISPR KO pooled library (76,441 gRNAs, targeting 19,114 genes, including 1,000 controls) was bought from Addgene (Watertown, MA, USA, Addgene ID: 73179). Plasmid library were amplified by electro-transformation (Lucigen ...
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bioRxiv - Cancer Biology 2023Quote: ... psPAX2 (Addgene 12260) and pVSVg (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... LentiCRISPR v2 (Addgene #52961) plasmid DNA (2 µg ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was packaged by co-transfecting transfer plasmids with pMD2.G (Addgene #12259) and pCMV dR8.2 (Addgene #12263 ...
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bioRxiv - Cancer Biology 2023Quote: ... and pCMV dR8.2 (Addgene #12263) to Lenti-XTM 293 cells (Takara Bio ...
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bioRxiv - Cancer Biology 2023Quote: An XRN1 open-reading frame (ORF) clone deposited by Elisa Izaurralde was purchased from Addgene (#66596). The entire ORF was sequenced to confirm fidelity to the NCBI Reference Sequence NM_019001.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Paul Thomas (Addgene plasmid #100900, RRID:Addgene_100900). The FKBP-WRN donor plasmid construct and WRN targeting vector were verified using Sanger sequencing (ACGT DNA Sequencing Services).
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bioRxiv - Cancer Biology 2023Quote: ... 53BP1-mCherry was excised mCherry-BP1-2 pLPC-Puro (Addgene plasmid #19835) (Dimitrova et al ...
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bioRxiv - Cancer Biology 2023Quote: Full length murine Tnf was PCR amplified from GFP-TNF-alpha plasmid (Addgene #28089) via Phusion PCR according to manufacturer’s protocol with the following primer ...
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bioRxiv - Cancer Biology 2023Quote: ... amplified from pAAVS1-NDi-CRISPRi (Gen1) (Addgene #73497) and cloned in via Pfl23II and MluI restriction sites for fluorescent-assisted cell sorting ...
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bioRxiv - Cancer Biology 2023Quote: The puromycin resistance cassette in the original CROPseq-Guide-Puro vector (Addgene #86708) was replaced with a mCherry sequence ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cells were transfected with viral packaging plasmids psPAX2 Addgene #12260) and pMD2.G (Addgene#12259), and pLKO.1 shRNA using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... pHAGE-PIK3CA-H1047L was a gift from Gordon Mills & Kenneth Scott (Addgene plasmid # 116499 ...
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bioRxiv - Cancer Biology 2023Quote: ... pHAGE-PIK3CA-H1047L was a gift from Gordon Mills & Kenneth Scott (Addgene plasmid # 116499; http://n2t.net/addgene:116499; RRID:Addgene_116499). pHAGE-AKT1-E17K was a gift from Gordon Mills & Kenneth Scott (Addgene plasmid # 116103).
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bioRxiv - Cancer Biology 2023Quote: Lentivirus particles were produced in the packaging cell line HEK293T using packaging vectors psPAX2 and pMD2.G (both from Addgene). Plasmids were transfected with polyethyleneimine (PEI ...
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bioRxiv - Bioengineering 2023Quote: ... ER (Addgene 37261), miniIAA7 (Addgene 129721) ...
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bioRxiv - Bioengineering 2023Quote: ... or a doxycycline-inducible minimal CMV promoter (Addgene 26431). An EGFP reporter was co-expressed with Aqp1 using an internal ribosome entry site (IRES ...