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Citations for Addgene :
8351 - 8400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... which was generated by replacing the EF1a-Puro cassette in Lentiguide-Puro (Addgene #52963)62 with a EF1a-Blasticidin cassette using the Xma/Mlu restriction sites ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... These plasmids were obtained from Addgene (DdCBE ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: The plasmids encoding ABE8e and ABE8e-V106W were obtained from Addgene (#138489 and #138495, respectively). The plasmid encoding ABE8e was modified to incorporate mutations in the adenine deaminase domain (V28R or R111S ...
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bioRxiv - Molecular Biology 2023Quote: The OSC cells were transfected with the pAc-sgRNA-Cas9Flag plasmid (Addgene plasmid #49330 ...
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bioRxiv - Molecular Biology 2023Quote: ... fused to a nuclear localization signal (NLS) and RNA guide was a gift from Jennifer Doudna (Addgene plasmid #195240; http://n2t.net/addgene:195240; RRID: Addgene_195240) (16) ...
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bioRxiv - Molecular Biology 2023Quote: pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid #186981 ...
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bioRxiv - Molecular Biology 2023Quote: pSpCas9(BB)-2A-Puro (PX459) was a gift from Feng Zhang (Addgene plasmid #48139 ...
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bioRxiv - Molecular Biology 2023Quote: pDF0159 pCMV - huDisCas7-11 mammalian expression plasmid was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid #172507; http://n2t.net/addgene:172507; RRID:Addgene_172507) (18) ...
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bioRxiv - Molecular Biology 2023Quote: pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid #186981; http://n2t.net/addgene:186981 ; RRID: Addgene_186981) (29) ...
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bioRxiv - Molecular Biology 2023Quote: pSpCas9(BB)-2A-Puro (PX459) was a gift from Feng Zhang (Addgene plasmid #48139; http://n2t.net/addgene:48139;RRID:Addgene_48139). Cas9 sgRNAs targeting the RTCB gene were cloned as previously described (table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Cas6) fused to a nuclear localization signal (NLS) and RNA guide was a gift from Jennifer Doudna (Addgene plasmid #195240 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Feng Zhang (Addgene plasmid #42230; [73]), respectively ...
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bioRxiv - Molecular Biology 2023Quote: Plasmids p2lox and pX330-U6-Chimeric_BB-CBh-hSpCas9 were kindly provided by Michael Kyba (Addgene plasmid #34635; [46]) and Feng Zhang (Addgene plasmid #42230 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Didier Trono (Addgene plasmid #12259; https://www.addgene.org/12259/; RRID: Addgene_12259), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... they were Gibson assembled into the pLenti-Rfx-guide-puro backbone digested with BsmBI (Addgene #138150). Library representation in the plasmid pool was confirmed by amplifying the gRNA cassette from the plasmid pool DNA and Illumina sequencing of the PCR product (Genewiz EZ AmpliSeq).
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bioRxiv - Molecular Biology 2023Quote: Oligonucleotides corresponding to individual gRNAs were synthesized (IDT) and cloned in the CasRx gRNA cloning backbone (Addgene #109053) using BbsI restriction sites ...
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bioRxiv - Molecular Biology 2023Quote: ... (Addgene Plasmid #83482 ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293FT cells were co-transfected with the Tet-on ssRFP-GFP-KDEL plasmid (Addgene 128257, Noboru Mizushima) and the lentivirus packaging plasmids psPAX2 (Addgene 12260 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the lentivirus packaging plasmids psPAX2 (Addgene 12260, Didier Trono) and pMD2.G (Addgene 12259 ...
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bioRxiv - Molecular Biology 2023Quote: ... The guide RNAs sequences were inserted into the pGL3-U6-sgRNA-PGK-puromycin plasmid (Addgene 51133, Xingxu Huang). U-2 OS ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa or HEK293FT cells were co-transfected with the sgRNA plasmid and the pST1374-NLS-flag-linker-Cas9 (Addgene 44758, Xingxu Huang). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... and pMD2.G (Addgene 12259, Didier Trono) using Lipofectamine® 2000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... a zebrafish codon optimized Cas9 (pT3TS-nCas9n, Addgene plasmid # 46757) was linearized with XbaI overnight and 1 μg of linear template DNA was used for in vitro transcription of mRNA using the T3 mMESSAGE mMACHINE® Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Feng Zhang (Addgene, Watertown, MA, USA, plasmid #62988; http://n2t.net/addgene:62988; RRID: Addgene_62988). pGP plasmid and pDON-5 Neo-ZsGreen plasmid were described previously [24].
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bioRxiv - Microbiology 2023Quote: ... single guide RNAs (sgRNAs) were ligated into an empty pLentiCRISPR plasmid backbone (gifted by Dr Feng Zhang; Addgene plasmid #52961 (46)) and packaged into lentivirus using XtremeGENE 9 DNA Transfection Reagent ...
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bioRxiv - Molecular Biology 2023Quote: ... pLenti-Guide-Puro (Addgene #52963 ...
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bioRxiv - Neuroscience 2023Quote: ... virus or AAV2- FLEX-sun1GFP (Addgene plasmid# 160141) control virus were packaged by SignaGen at high-titer (1 × 103 gc/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... Wilson (Addgene plasmid # 112867 ; http://n2t.net/addgene:112867 ; RRID:Addgene_112867).
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bioRxiv - Molecular Biology 2023Quote: ... Cas9-mRNA was in vitro transcribed overnight at 20°C from 400-500 ng XbaI-linearized pT3TS-nCas9n (Addgene, plasmid #46757) using the mMessage mMachine T3 Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... coding sequences of GFP-NLS-tetR fusion protein was synthesized and cloned into UAS expression plasmid pJFRC-MUH (Addgene #26213) by GenScript Biotech ...
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bioRxiv - Molecular Biology 2023Quote: dCas9-VPR and Cas9 experiments were performed using a published multiplex four sgRNA construct system.79 The four sgRNAs for each of the sgRNA target sites (Kcnk9 TSS, E1, and E2) were cloned into four sgRNA backbones each containing different small RNA promoters (Addgene #53186, #53187, #53188, #53189). Golden Gate cloning into pLV-GG-hUbC-dsRED (Addgene #84034 ...
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bioRxiv - Molecular Biology 2023Quote: ESCs were co-transfected with 1000 ng of pX458 SpCas9(BB)-2A-GFP vector (Addgene #48138, contains eGFP)80 and 500 ng of vector co-expressing four sgRNAs to the Peg13 DMR CTCF region (contains DsRed ...
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bioRxiv - Molecular Biology 2023Quote: WT and mutant firefly luciferase reporters were made based on the backbone plasmid purchased from Addgene (https://www.addgene.org/114670/). PCR amplified the 5’ UTR DNA fragments from cDNA prepared from HEK293T or AC16 human CM cell line using primers with the extra 5’ end corresponding to the BsmbI cut sites in the plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... Golden Gate cloning into pLV-GG-hUbC-dsRED (Addgene #84034) generated the final vector co-expressing four sgRNAs.
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bioRxiv - Molecular Biology 2023Quote: 2 µg total of multiplex sgRNA vector and EF1α-dCas9-VPR-Puro vector (Addgene #99373) at a 2:1 molar ratio were mixed with 5 µl of Lipofectamine 2000 (Thermo ...
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bioRxiv - Neuroscience 2023Quote: We obtained pAAV-Ef1a-FLPo (Addgene plasmid #55637 ...
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bioRxiv - Molecular Biology 2023Quote: ... pUGI-NLS UDG Inhibitor (UGI) plasmid was purchased from Addgene (Cat#101091).
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bioRxiv - Molecular Biology 2023Quote: Cells were plated in 10-cm plates and transfected with plasmids expressing His6-SUMO1 (Addgene plasmid # 133770) or His6-SUMO2 (Addgene plasmid # 133771) ...
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bioRxiv - Molecular Biology 2023Quote: ... or His6-SUMO2 (Addgene plasmid # 133771). 24 h post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 × 106 293T cells were seeded in a 10-cm plate 1 d prior to transfection and co-tranfected with the 3rd generation lentiviral packaging plasmids (5 µg pVSV.G, 3 µg pMDLg/pRRE, and 2.5 µg pRSV-Rev; Addgene # 14888 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA oligonucleotides containing sgRNAs were cloned into LentiCRISPRv2 (Addgene # 52961) using BsmBI ...
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bioRxiv - Molecular Biology 2023Quote: ... two different gRNAs for SETD6 (Table 2) were cloned into lentiCRISPR plasmid (Addgene, #49535). Following transduction and puromycin selection (2.5 µg/ml) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the second-generation packaging plasmid psPAX2 (Addgene #12260) into Hek293T cells (ATCC ...
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bioRxiv - Molecular Biology 2023Quote: ... “HCT116 Cas9” or “K562 Cas9”) were generated by lentiviral transduction of the lenti.Cas9.BFP.Blast plasmid (Addgene #196714) and selected with 5 μg/ml blasticidin (Invivogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and pCMV-VSV-G (a gift from Bob Weinberg, Addgene #8454) in HEK-293T (ATCC) ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid library was packaged into the lentivirus utilising plasmids psPAX2 (a gift from Didier Trono, Addgene #12260) and pCMV-VSV-G (a gift from Bob Weinberg ...
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bioRxiv - Molecular Biology 2023Quote: This construct was then cloned into the pLenti-Puro-AU-flip-3xBsmBI (Addgene #196709) (75 ...
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bioRxiv - Neuroscience 2023Quote: ... University of Zurich (purified by VVF as v723-9; AAV9-hCMV-HA-SpCas9, Addgene 106431, gift from Juan Belmonte [22]).
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bioRxiv - Neuroscience 2023Quote: ... the p415 TEF roGFP2-Tsa2ΔCR plasmid (Addgene #83238)21 was digested by NotI and XbaI ...
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bioRxiv - Neuroscience 2023Quote: ... a 1 μL dot of AAV9/hSyn/GCaMP6f virus (Addgene, Watertown, MA) diluted 1-5x from commercial titer (2.8x1013 to ∼1x1012 units/mL ...