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Citations for Addgene :
8701 - 8750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... This pl452-puroTK vector was derived from plasmids pl452 and plasmid pLCA.66/2272 82 (Addgene plasmid #22733) by removing the PGK-puroTK cassette with AgeI and BbsI and cloning that fragment into pl452 using the same enzymes ...
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bioRxiv - Bioengineering 2023Quote: ... Pae Type I Cascade plasmids encoding Csy1-Csy2 (Addgene #153942) and Csy3-VPR-Csy4 (Addgene #153943 ...
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bioRxiv - Bioengineering 2023Quote: ... MS2-modified gRNA expression plasmid (Addgene #61424), AsCas12a (Addgene #128136) ...
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bioRxiv - Bioengineering 2023Quote: ... the scFv-GCN4-linker-VP16-GB1-Rex NLS sequence was PCR amplified from pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS (Addgene #60904) and cloned into a lentiviral backbone containing an EF1-alpha promoter ...
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bioRxiv - Bioengineering 2023Quote: ... SpgRNA expression plasmid (Addgene #47108), MS2-modified gRNA expression plasmid (Addgene #61424) ...
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bioRxiv - Bioengineering 2023Quote: ... GG-EBNA-OSK2M2L1-PP (Addgene #102898) and GG-EBNA-EEA-5guides-PGK-Puro (Addgene #102898 ...
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bioRxiv - Bioengineering 2023Quote: ... Coli Type I Cascade system (Addgene #106270-106275) and Pae Type I Cascade System (Addgene #153942 and 153943) ...
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bioRxiv - Bioengineering 2023Quote: ... SiT-Cas12a-Activ (Addgene #128136) was used ...
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bioRxiv - Bioengineering 2023Quote: ... hSyn-AAV-EGFP (Addgene #50465) plasmid was used to generate different AAV based DNA constructs ...
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bioRxiv - Bioengineering 2023Quote: ... CjCas9 was PCR-amplified from pAAV-EFS-CjCas9-eGFP-HIF1a (Addgene #137929) as two overlapping fragments using primers to create D8A and H559A mutations ...
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bioRxiv - Bioengineering 2023Quote: ... 1x 9aa TADs were designed and annealed as double strand oligos and then cloned into the BamHI/NheI-digested MCP-p65-HSF1 backbone plasmid (Addgene #61423) using T4 ligase (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... and Pae Type I Cascade System (Addgene #153942 and 153943), YAP-S5A (Addgene #33093 ...
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bioRxiv - Bioengineering 2023Quote: ... Selected fusions between 3x 9aa TADs and eNRF2 were generated using gBlock (IDT) fragments and cloned into the BamHI/NheI-digested MCP-p65-HSF1 backbone plasmid (Addgene #61423) using NEBuilder HiFi DNA Assembly ...
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bioRxiv - Bioengineering 2023Quote: ... the MCP-p65-HSF1 plasmid (Addgene #61423) was digested with BamHI and NheI and respective effector domains were cloned using NEBuilder HiFi DNA Assembly ...
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bioRxiv - Bioengineering 2023Quote: ... AsCas12a (Addgene #128136), E ...
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bioRxiv - Bioengineering 2023Quote: ... and GG-EBNA-EEA-5guides-PGK-Puro (Addgene #102898) used for reprogramming experiments have been described previously17 ...
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bioRxiv - Bioengineering 2023Quote: ... and cloned into chimeric U6 promoter containing sgRNA cloning plasmid (Addgene #47108) and/or an MS2 loop containing plasmid backbone (Addgene #61424 ...
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bioRxiv - Bioengineering 2023Quote: ... the dCas9-p300 plasmid (Addgene #83889) was digested with BamHI and then a synthetic double-stranded ultramer (IDT ...
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bioRxiv - Bioengineering 2023Quote: ... were obtained from Addgene. The Csy3-VPR-Csy4 plasmid was digested with MluI (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... scFv-VP64 (Addgene #60904), SpgRNA expression plasmid (Addgene #47108) ...
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bioRxiv - Bioengineering 2023Quote: ... The pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP (Addgene #60903) vector was used for dCas9-based scFv fusion protein recruitment to target loci ...
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bioRxiv - Bioengineering 2023Quote: ... and Csy3-VPR-Csy4 (Addgene #153943) were obtained from Addgene ...
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bioRxiv - Bioengineering 2023Quote: ... dCas9-p300 (Addgene #83889), MCP-p65-HSF1 (Addgene #61423) ...
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bioRxiv - Neuroscience 2023Quote: mCherry-Cre (Addgene no. 27546) and mCherry-only vectors are described in Kirby et al. ...
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bioRxiv - Neuroscience 2023Quote: ... we used pAAV9.CAG.Flex.GCaMP6s.WPRE.SV40 (1.9 x 1013 vg/ml, Addgene) or pGP-AAV1-syn-FLEX-jGCaMP7f-WPRE (titre 2.1 x 1013 vg/ml ...
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bioRxiv - Biophysics 2023Quote: ... generated by us in the Kiel center [51] using a modified version of the pHyVec1 plasmid which replaces the GFP sequence with a GCaMP6s sequence that was codon-optimized for Hydra (HyGCaMP6s was a gift from R. Yuste lab (Addgene plasmid # 102558; http://n2t.net/addgene:102558 ; RRID:Addgene_102558) [37]) ...
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bioRxiv - Neuroscience 2023Quote: Climbing fibers (CF) were targeted28,48 by injecting 250nl of AAV1.CaMKIIa.hChR2(H134R)-mCherry.WPRE.hGH (Addgene#26975110) or AAV8.CamKII.Jaws-KGC.GFP.ER2-WPRE.SV40 (UPen#AV-8-PV363778 ...
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bioRxiv - Biophysics 2023Quote: ... generated by us in the Kiel center [51] using a modified version of the pHyVec1 plasmid which replaces the GFP sequence with a GCaMP6s sequence that was codon-optimized for Hydra (HyGCaMP6s was a gift from R. Yuste lab (Addgene plasmid # 102558 ...
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bioRxiv - Neuroscience 2023Quote: ... or pGP-AAV1-syn-FLEX-jGCaMP7f-WPRE (titre 2.1 x 1013 vg/ml, Addgene). 6s and 7f dopamine recordings were pooled in presented analysis ...
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bioRxiv - Pathology 2023Quote: ... pSB plasmids were introduced together with pCMV(CAT)T7-SB100 (Addgene) using Effectene (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: WNT3A and WNT4 were sub-cloned by Gateway recombination from the open-source Wnt library (Addgene Kit #1000000022) to the MAC-Tag-C vector (Addgene #108077 ...
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bioRxiv - Neuroscience 2023Quote: AAV1-Syn-Flex-GCaMP6f-WPRE-SV40 was a gift from Douglas Kim and the GENIE Project (Addgene viral prep # 100833-AAV1 ...
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bioRxiv - Neuroscience 2023Quote: ... AAVretro-CaMKIIa-mCherry was a gift from Karl Deisseroth (Addgene viral prep # 114469-AAVrg; http://n2t.net/addgene:114469; RRID: Addgene_114469). AAV1-EF1a-N-CretrcintG and AAV1-EF1a-C-CreintG were gifts from Connie Cepko (Addgene viral prep # 69570-AAV1 and 69571-AAV1 ...
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bioRxiv - Immunology 2023Quote: ... which targets the reverse strand of the first exon) was inserted into pspgRNA31 (a gift from Charles Gersbach, Addgene plasmid # 47108). 293T cells were co-transfected with equal amounts of this plasmid and pCas9_GFP32 (a gift from Kiran Musunuru ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The pH1R-P2A-mCherry-N1 was a gift from Dorus Gadella (Addgene plasmid # 84330) (41) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The CMV-hEAAT3 plasmid was a gift from Susan Amara (NIMH; Addgene plasmid #32815). The pH1R-P2A-mCherry-N1 was a gift from Dorus Gadella (Addgene plasmid # 84330 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The pET9Bc expression vector was obtained from the Addgene nonprofit plasmid repository and was gifted to the repository by Scott Gradia (Addgene plasmid # 48285; http://n2t.net/addgene:48285; RRID: Addgene_48285). For protein expression ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... employing the construct pIRESneo-EGFP-alpha Tubulin (a gift from Patricia Wadsworth; Addgene plasmid #12298 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The effect of CPC on plasma membrane potential and cytosolic pH were determined in tandem using the pH-sensitive genetically-encoded voltage indicator (GEVI) ArcLight-A242 (a gift from Vincent Pieribone; Addgene plasmid # 36857 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RBL-2H3 cells were transfected with the mitochondrial calcium indicator pCMV CEPIA2mt (a gift from Masamitsu Iino; Addgene plasmid # 58218; http://n2t.net/addgene: 58218; PRID:Addgene_58218) (Suzuki et al. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RBL-2H3 cells were transiently transfected with the genetically encoded Ca2+ indicator pGP-CMV-GCaMP6f (a gift from Douglas Kim & GENIE Project; Addgene plasmid # 40755; http://n2t.net/addgene:40755; RRID:Addgene_40755), (Chen et al. ...
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bioRxiv - Neuroscience 2023Quote: Plasmids containing pAAV-hsyn-ChrimsonR-tdTomato (Addgene # 59171) (51 ...
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bioRxiv - Microbiology 2023Quote: ... cells were transduced with the respective lentivirus (pWPI-IRES-Puro-Ak-ACE2, kindly provided by Dr. Sonja Best, Addgene viral prep # 154985-LV) at MOI 0.3 ...
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bioRxiv - Microbiology 2023Quote: ... pLVX-EF1a-SARS-CoV-2-nsp16-IRES-puro was generated by subcloning the nsp16 coding sequence from pDONR223 SARS-CoV-2 NSP16 (Addgene #141269) into pLVX-EF1a-IRES-puro empty vector ...
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bioRxiv - Microbiology 2023Quote: ... and pDONR223 SARS-CoV-2 spike (Addgene #149329) were subcloned into pLVpuro-CMV-N-3xFLAG (Addgene #123223 ...
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bioRxiv - Microbiology 2023Quote: ... pLVX-EF1a-IRES-puro empty vector was generated by swapping the eGFP insert of pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro (Addgene #141395) with a multiple cloning site ...
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bioRxiv - Microbiology 2023Quote: ... sequences were subcloned into pInducer20 (Addgene #44012) via Gateway cloning.
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bioRxiv - Microbiology 2023Quote: 4e5 VeroE6 cells were plated in 6-well plates and transfected with 1 μg VSV-G plasmid (Addgene #8454) via TransIT-X2 (Mirus) ...
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bioRxiv - Immunology 2023Quote: ... and inserted into a generic targeting vector (pBigT, Addgene, #21270), pBigT-mSparcl1 was then subsequently cloned into a plasmid with the ROSA26 genomic flanking arms (ROSA26-PA ...
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bioRxiv - Immunology 2023Quote: CTLs were transiently transfected with the pEGFP plasmid encoding the EB1-GFP fusion protein (Addgene #17234, Watertown, Massachusetts, USA). Briefly ...