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Citations for Takara Bio :
4701 - 4750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and 2 μg/ml doxycycline (Clontech, cat. no. 631311). Neuronal induction media was changed once a day for 2 more days ...
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bioRxiv - Molecular Biology 2023Quote: ... retrotranscribed using PrimeScript Reagent Kit (Takara) and amplified by RT-qPCR using PowerUp SYBR-Green MasterMix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: Human neurons were transfected on DIV28 with pEGFP-C1 (0.3 μg/well, Clontech) plus vectors expressing mCherry ...
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bioRxiv - Biochemistry 2023Quote: ... The digested XLone vector was then fused with the insert of the respective constructs by in-fusion cloning method according to the manufacturer’s instructions (Takara Bio Inc.). The resulting products from the respective in-fusion cloning reactions were transformed into stbl3 One-Shot competent cells according to the manufacturer’s instructions (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... For amplification of DNA sequences for cloning and NGS the PrimeSTAR Max DNA polymerase (Takara Bio Inc.) and for colony PCRs the OneTaq Quick-Load Master Mix Polymerase was used (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... and inserted into a modified pEGFP-N1 vector (Clontech) in which the downstream EGFP gene was deleted and an HA tag was added at the C-terminus ...
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bioRxiv - Biochemistry 2023Quote: ... the PrimeSTAR Max DNA Polymerase (Takara Bio Inc.) was used according to the manufacturer’s recommendation with a 25 µl reaction volume ...
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bioRxiv - Biochemistry 2023Quote: ... and reverse transcribed according to the manufacturer‘s protocol (Takara). qRT-PCR was carried out using SYBR Premi qRT-PCR ExTaq™ II (Takara ...
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bioRxiv - Biochemistry 2023Quote: ... Lentivirus was concentrated by adding 333 μL Lenti-X concentrator (Takara, cat# 631231) per mL of viral supernatant ...
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bioRxiv - Biochemistry 2023Quote: The retroviral titre calculation was done using the Retroviral titration kit from Takara Biosciences (Retro-X™ qRT-PCR Titration Kit 631453 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was collected and concentrated using Retro-X-Concentrator from Takara Biosciences (Takara #631455 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 125 μL of TALON cobalt resin (Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: ... inserting an in-frame 15bp flexible linker sequence (encoding the amino acids GGGGA) followed by EGFP at the C-terminus using Infusion cloning (Clontech). For the Tg(NBT:ap2s1-gfp ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-st PCR (PCR 1) was performed using Titanium Taq DNA Polymerase (# 639209, Takara Bio, CA, USA). Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: ... Genomic DNA was extracted from cell pellets using the Machery Nagel NucleoSpin Blood Kit (Midi, Clontech Cat ...
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bioRxiv - Systems Biology 2023Quote: ... Genomic DNA was extracted from cell pellets using the Machery Nagel NucleoSpin Blood Kit (Midi, Clontech Cat. #740954.20; XL Maxi, Clontech Cat. #740950). The detailed protocol is available at http://www.broadinstitute.org/rnai/public/resources/protocols.
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bioRxiv - Biochemistry 2023Quote: ... All site directed mutagenesis reactions were performed using PrimeSTAR HS DNA Polymerase (Takara Bio, cat #R010B). Plasmids used for transient transfections in HEK293T cells were prepared and purified using the Zymopure Midiprep Kit (Zymo Research ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells were transiently transfected with the expression control vector pCMV-TRE3G (Clontech, Mountain View, CA, USA) and the coexpression construct pTRE3G-BI/GPR83-LgBiT:SmBiT-ARRB2 that encodes a C-terminally LgBiT-fused human GPR83 (LgBiT-GPR83 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was collected and concentrated using Retro-X-Concentrator from Takara Biosciences (Takara #631455) overnight at 40C ...
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bioRxiv - Biochemistry 2023Quote: ... GFP (JL-8, Clontech) or Vinculin (7F9 ...
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bioRxiv - Biochemistry 2023Quote: ... GFP expression was verified using immunoblotting using GFP (JL-8, Clontech) and α-tubulin (CP06 ...
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bioRxiv - Cell Biology 2023Quote: In-fusion cloning was performed using the In-Fusion HD EcoDry Cloning Plus kit (Takara), and standard cut-and-paste cloning using T7 DNA ligase (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... cDNA was amplified using SYBR Premix ExTaq (Takara, Madison, WI, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: pET28a-Halotag-CNA35 was created using InFusion HD Assembly (Takara) by replacement of the eGFP coding sequence within pET28a-eGFP-CNA3546 (Addgene ...
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bioRxiv - Plant Biology 2023Quote: Binary vectors for agrobacterium-mediated transformation were constructed using the backbone of pRI-201 (TaKaRa Bio) or pGWB (Nakagawa et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purification of Ypq1-3C-GFP-8xHis was done by incubating the supernatant with 3 mL TALON cobalt resin (635502; TaKaRa Bio) pre-equilibrated with Column buffer (20 mM HEPES-KOH (pH 7.2) ...
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bioRxiv - Biochemistry 2023Quote: ... Soluble material was incubated with His60 Superflow resin (Clontech) for 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Viral titer was quantified using the Lenti-X™ qRT-PCR Titration Kit (Takara Bio, #631235), according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... cells were exchanged with fresh media containing B/B homodimerizer (500nM) (Takara Bio, #635059) and/or Baf-A1 (100nM ...
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bioRxiv - Biochemistry 2023Quote: ... MBP-Hua2(K221WY -AWY)NT-FLAG and MBP-Hua2(G183KV-AAV)NT-FLAG were constructed by introducing point mutations using In-Fusion HD Cloning (Takara Bio USA, Inc) with the primers listed in Table 1 ...
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bioRxiv - Genetics 2023Quote: ... and cloned these into expression vectors using Infusion cloning (In-Fusion HD Cloning Plus, Cat. 638911, Takara Bio USA.)
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bioRxiv - Immunology 2023Quote: ... linearized BF520 sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... and concentrated with Lenti-X concentrator (Clontech). The 293fT cell line was not authenticated in the laboratory ...
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bioRxiv - Biophysics 2023Quote: ... was coated with retronectin (Takara Bio, ref: T100B) in PBS (7 μg/ml ...
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bioRxiv - Genetics 2023Quote: ... the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio, Inc.) was used for cDNA library preparation ...
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bioRxiv - Immunology 2023Quote: ... HEK293T (ATCC collection) and GP2–293 packaging cell lines (Clontech) were cultured in DMEM (10938-025 ...
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bioRxiv - Immunology 2023Quote: T cell receptors were generated by custom synthesis (Twist Bioscience) in a 3rd generation pLVX-EF1α-IRES-Puro lentiviral vector (Takara/Clontech). Following the TransIT-Virusgen Transfection (Mirus ...
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bioRxiv - Immunology 2023Quote: T cell receptors were generated by custom synthesis (Twist Bioscience) in a 3rd generation pLVX-EF1α-IRES-Puro lentiviral vector (Takara/Clontech). Following the TransIT-Virusgen Transfection (Mirus ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products of the exons were assembled into one full-length sequence using overlapping PCR (In-fusion cloning, Clontech) for each TAS1R and were then subcloned into the pEAK10 expression vector (Edge Biosystems ...
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bioRxiv - Immunology 2023Quote: Non-tissue culture treated 12-well plates were coated overnight at 4°C with 1 ml Retronectin (Takara) at 25 μg/ml in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were rinsed four times for 15 min each with 0.1 M PBS and incubated with secondary antibodies anti-DsRed (1:500; RRID:AB_10013483; Living Colors DsRed polyclonal; Takara Bio USA, San Jose, CA) and anti-Troma1 (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... a PCR test is performed using the Terra PCR Direct Polymerase Mix (Takara Bio Europe SAS ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were purified from the soluble cell lysate using His60 Ni Superflow Resin (Takara Bio) and concentration was measured using NanoDrop ...
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bioRxiv - Bioengineering 2023Quote: ... The synthesized gene fragments were then cloned into the linear pET21a vector using the In-Fusion® HD Cloning Kit (Takara Bio, Kusatsu, Japan). For mammalian expression ...
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bioRxiv - Cell Biology 2023Quote: ... the plasmid was constructed using the In-Fusion HD Cloning Kit (Takara); 1–2 kb sequences of the 5’ and 3’ ends of the genes of interest flanked the fragment that consisted of an in-frame linker ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA using the indicated primers (Table S1).Fluorescent reporters were ordered as gBlocks and cloned using the In-fusion HD EcoDry Cloning kit (Takara). Promoter reporter constructs were created as previously described (11 ...
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bioRxiv - Microbiology 2023Quote: pZIP transfer vectors were packaged in Lenti-X 293T cells (Takara) using molar ratios of 0.2 transfer vector to 0.51 psPax2 to 0.29 pMD2.G ...
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bioRxiv - Microbiology 2023Quote: ... S1A were cloned into pMSCVpuro (Takara, Catalog # 631461) by PCR ...
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bioRxiv - Neuroscience 2023Quote: ... mixed with Lenti-X concentrator (Takara), and stored at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... concentrated lentiviral titer was estimated by Takara GoStix and GV ∼25,000 (cpTurboID ...