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Citations for Takara Bio :
3451 - 3500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 2 µg pantropic pVSV-G (Clontech), 3 µg pCL- (Imgenex) ...
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bioRxiv - Bioengineering 2023Quote: ... via retroviral infection with the pRetroX-Tet-Off Advanced (Clontech) vector and selection with 1 mg/ml G418 ...
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bioRxiv - Bioengineering 2023Quote: ... CHAMP-1 or ErbB2TM cloned in the expression vector pRetroX-TIGHT-puro (Clontech) was introduced into cells expressing tTA by retroviral infection and selection with 1 µg/ml puromycin ...
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bioRxiv - Bioengineering 2023Quote: ... qRT-PCR was performed with TB Green Premix Ex Taq II (Takara RR820L). Each 20-μl reaction contained 2 μL of cDNA and 10 μL of TB Green solution with a final primer concentration of 200 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... the target mRNA CDS sequence was cloned using PrimeSTAR® Max (TAKARA, R045) or Q5® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... the fragments were subcloned into the pLVX-puro vector (Takara, #632164) with Ha- or Flag-tag ...
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bioRxiv - Cancer Biology 2023Quote: ... Wildtype and mutant constructs were then PCR amplified using primers to encompass HA-EZH2 + add NotI and PacI for subcloning into pQXCIH (Clontech). See Supplemental Table 1 for the primer sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... The adherent cells HEK293T-LentiX (TAKARA, Mountain View, CA) were grown in DMEM (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... First strand cDNA was synthesized using the Primescript RT Reagent Kit (TaKaRa) and 0.5 μg of total RNA in each cDNA synthesis reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA isolation and real-time PCR assay Total RNA was extacted by RNAiso Plus (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... coli competent cells (Takara Bio), from which single colonies were isolated from LB plates supplemented with ampicillin ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed according to the manufacturer’s protocol (TAKARA, Cat. No. RR047A) using 1 µL of the lysate as reverse transcriptase and TRIzol reagent-isolated 293T total RNA as template ...
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bioRxiv - Microbiology 2023Quote: ... CloneAmp HiFi polymerase (Takara) was used ...
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bioRxiv - Microbiology 2023Quote: ... 1µl (250U) PrimeStarGXL (TAKARA), and 5 μl of 10 μmol/μl “FWD 2 Deep seq” and “Rev Deep seq” primers (table S5) ...
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bioRxiv - Microbiology 2023Quote: ... 1µl (250U) PrimeSTAR GXL DNA polymerase (TAKARA), 4µl 2.5mM dNTP’s ...
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bioRxiv - Immunology 2023Quote: ... Transduction was performed on non-tissue culture 24-well plates previously coated with 0.5mL of RetroNectin (Takara) at a concentration of 25 μg/mL in PBS ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus) (Takara, RR420B) according to manufacturer’s instructions on an Applied biosystems StepOnePlus machine ...
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bioRxiv - Immunology 2023Quote: ... and 1 µg was used for cDNA synthesis with the PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). Of the cDNA ...
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bioRxiv - Microbiology 2023Quote: ... reverse transcription was performed by using the PrimeScript RT Kit (Takara Bio, San Jose, CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription and tagmentation were conducted using the SMART-Seq v4 (Takara) and Nextera XT library prep kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: The 5’ and 3’ termini of the sRNA OueS were determined by the RACE assay (SMARTer RACE 5’/3’ kit, Takara Bio USA), adapted for non-poly-A-tailed RNA ...
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bioRxiv - Microbiology 2023Quote: ... Viral stocks were concentrated with the Lenti-X Concentrator (TaKaRa #631232).
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bioRxiv - Immunology 2023Quote: ... and TB Green Premix Ex Taq (Tli RNaseH Plus) (Takara, RR820W) with Quantstudio6 Flex Real Time PCR system (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... or PrimeScript RT reagent Kit (Takara, RR037A) and TB Green Premix Ex Taq (Tli RNaseH Plus ...
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bioRxiv - Molecular Biology 2023Quote: ... and mmpL3 were amplified from Mtb H37Rv genomic DNA by PCR and inserted into pGW1-6C (gift of Tom Alber, University of California, Berkeley) by InFusion (Takara Bio) for wild-type genes or by ligation for recoded gene variants using restriction sites SpeI and NdhI ...
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bioRxiv - Molecular Biology 2023Quote: ... or into pHTN-HaloTag vector for GID4-HaloTag N-terminal fusion using the In-Fusion HD Cloning kit (Takara). Pro/N-degron coding sequence was cloned into pNLF1-C for MPGLWKS-NanoLuc C-terminal fusion ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA libraries were generated using the SMART-Seq Stranded Kit (Takara). The kit incorporates SMART® cDNA synthesis technology81 and generates Illumina-compatible libraries via PCR amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... Matchmaker yeast two-hybrid library of arabidopsis cDNA purchased from Clontech. CD4-22 and CD4-10 libraries were converted from λ cDNA into plasmid libraries by transformation into BNN132 bacterial cells from which the plasmids were extracted ...
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bioRxiv - Molecular Biology 2023Quote: ... and 50 μg of denatured Yeastmaker™ Carrier DNA (Clontech). The cells were incubated with PEG4000 buffer (40% [w/v] PEG4000 ...
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bioRxiv - Molecular Biology 2023Quote: ... but was tested in a directed two-hybrid assay in which cDNAs encoding ATI1 and ATI2 were cloned into the prey vector pGADT7 (Clontech) and co-transformed into PJ69-4A with the NcGNAGO1 bait plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... His (Clontech), supplemented with 1.5 mM 3-amino-1,2,4-triazol (3-AT ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µg of RNA per sample were incubated with 5U MNase (Takara Bio, 2910A) supplemented with 5 mM CaCl2 in 200 µl final volume ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed by using Premix Probe Ex Taq or SYBR-green (Takara) and commercially available probes from Applied Biosystems (GAPDH ...
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bioRxiv - Molecular Biology 2023Quote: ... and fragments were cloned into the pSAM2-Puro lentiviral vector 13 by In-Fusion HD Cloning (Takara).
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bioRxiv - Molecular Biology 2023Quote: ... The cells were washed twice in DMEM containing 0.001 U/µL DNase I (Takara, 2270A) and 5% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... the first-strand cDNA was synthesized from 1 μg total RNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... 10 μL of TB Green Premix Ex Taq 2 (TliRNaseH Plus) (Takara, Beijing, China), 0.8 μL of 10 μM bidirectional primers (Table 1) ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloned in between the XhoI and SalI sites of psiCHECK-2.2 using InFusion cloning (TaKaRa Bio Inc.). To create mutations to the predicted miR-151a binding sites ...
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bioRxiv - Molecular Biology 2023Quote: ... and then cloned into the XbaI site of pHIV7/PGK-neo using InFusion cloning (TaKaRa Bio Inc.) to create the pHIV7/sli-miR-151a-3p-PGK-neo ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was gel purified using the Nucleospin Gel and PCR Clean-Up Kit (TaKaRa Bio Inc.) and cloned in between the XhoI and SalI sites of psiCHECK-2.2 using InFusion cloning (TaKaRa Bio Inc.) ...
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bioRxiv - Molecular Biology 2023Quote: ... gel purified using the Nucleospin Gel and PCR Clean-Up Kit (TaKaRa Bio Inc., Kusatsu, Japan), and then cloned into the XbaI site of pHIV7/PGK-neo using InFusion cloning (TaKaRa Bio Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... the libraries were prepared by SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input (TaKaRa). The other RNA-Seq libraries were generated by a TruSeq Stranded mRNA Library Prep kit (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... and those targeting the Alu repeat sequence in the nuclear genome (5′-CTTGCAGTGAGCCGAGATT-3′ and 5′- GAGACGGAGTCTCGCTCTGTC-3′) (75) with TB Green Premix Ex Taq II (TaKaRa) on Thermal Cycler Dice Real Time System II (TaKaRa) ...
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bioRxiv - Molecular Biology 2023Quote: ... on Thermal Cycler Dice Real Time System II (TaKaRa). The relative mitochondrial DNA content normalized by nuclear-genome content was determined using the ΔΔCt method.
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μM of Shield-1 peptide (Clontech, Mountain View CA) for 4 h ...
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bioRxiv - Molecular Biology 2023Quote: A yeast two-hybrid bait plasmid was constructed by cloning an AGO1 cDNA fragment encoding residues Q165-Y335 (NcGNAGO1) into the pGBKT7 vector (Clontech). Three libraries were used for the yeast two-hybrid screen of candidates interacting with AGO1 NcGN ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then treated with 1 µM Shield1 (Takara Bio Cat#632189) 5 days post-BFP sort then harvested for flow cytometry and Western blot analysis 3 days post-Shield1 treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were prepared with sheared DNA (0.5 ng of ChIP DNA or 5.0 ng of input DNA) using ThruPLEX® DNA-seq Kit (Clontech). These obtained libraries were size-selected at 300-700 bp using ×1.0 AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2023Quote: ... The reverse transcription and qRT-PCR kits were purchased from Takara; Trizol was purchased from ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). Transfected cells were cultured in selective media with 100 μg/ml of hygromycin B for 2 weeks ...