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Citations for Takara Bio :
3401 - 3450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara). Finally ...
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bioRxiv - Microbiology 2023Quote: ... 293T cells were cotransfected with 2.4 μg of proviral DNA construct and 0.6 μg of VSV-G expression vector using TransIT-LT1 reagent (Takara, Cat# MIR2306) into 293T cells (3 × 106) ...
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bioRxiv - Molecular Biology 2023Quote: ... and treated by DNase I (TaKaRa, Japan). The total RNAs were sent to Macrogen Japan Corp ...
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bioRxiv - Molecular Biology 2023Quote: The viral particles were concentrated by precipitation using the Lenti-X concentrator (Clontech) and resuspended in cell culture medium ...
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bioRxiv - Molecular Biology 2023Quote: Site-directed mutagenesis in PTBP2 and KIS cDNAs was performed by In-Fusion HD (Takara, 638909). pcDNA3-FLAG ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 ug of RNA was reverse-transcribed into cDNAs using the Prime Script RT Reagent Kit (Takara, RR037B) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Retroviral supernatants were filtered and concentrated with Lenti-X concentrator (Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA ligations were performed using the In-Fusion HD Cloning Kit (Takara Bio). The identity of all constructs and mutant strains was confirmed by sequencing (Microsynth AG) ...
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bioRxiv - Molecular Biology 2023Quote: ... Stellar competent cells (Takara Bio) provided with the In-Fusion Cloning Kit were used in all cloning procedures ...
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bioRxiv - Microbiology 2023Quote: ... Mighty Mix (Takara Bio) was used for ligation ...
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bioRxiv - Microbiology 2023Quote: ... The DNA samples (10 μg each) were digested with EcoRI (Takara Bio) and SalI (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... lytic-induced or uninduced cells (5×105 cells on a 6-well plate) were harvested with 500 μL of RNAiso Plus (Takara Bio). Total RNA was extracted ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates were applied to 3 mL TALON Metal Affinity Resin (Takara) and incubated for 30 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The single isoform of DCR1 was amplified by RT-PCR from mRNA isolated from S2 cells and reverse transcribed using the Oligo(dT)-primed RNA to cDNA EcoDry premix (Takara Bio). The CDS for AGO1 and GW182 were PCR amplified from pAFW-Ago1 (Addgene #50553 ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was then reverse transcribed using the double primed RNA to cDNA EcoDry premix (Takara Bio). RNA abundance was determined by qPCR using primers targeting FLuc ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using the double primed RNA to cDNA EcoDry premix (Takara Bio). Primers were designed targeting FLuc ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed on a thermal cycler (TaKaRa) using TB Green Premix Ex TaqTM II (TaKaRa ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviral titration was performed by RT-qPCR using the Lenti-X qRT-PCR Titration Kit (Takara) and the concentration of lentivirus used for this paper was found to be above 1 × 107 copies / mL ...
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bioRxiv - Cell Biology 2023Quote: ... Individual master mixes for each gene of interest were prepared using the TB Green® Premix Ex Taq II (TLi RNaseH Plus) reagent kit (RR820A, Takara Bio) and primer sets ordered from Sigma-Aldrich (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2023Quote: ... Entry clones for targeting and interaction assessment in mammalian cell culture lines were shuttled into the appropriate destination vectors including: pEGFP-N3 (Clontech), pmCherry-C1 (Clontech) ...
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bioRxiv - Bioengineering 2023Quote: ... GP2-293 cell line (Takara, CA) was used as the viral packing cell line for plasmid encoding CAR construct ...
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bioRxiv - Developmental Biology 2023Quote: ... Lenti-X cells were purchased from Takara and maintained in DMEM containing 10% FBS ...
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bioRxiv - Plant Biology 2023Quote: ... were PCR-amplified and fused to a nuclear localization signal (NLS) in pENTR vectors using In-Fusion HD Cloning Plus (Clontech). See Table S5 for primer details ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative real-time RT-PCR analysis was performed using TB Green Premix Ex Taq (Clontech, RR420A, Mountain View, CA, USA). The following diluted primers were used ...
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bioRxiv - Microbiology 2023Quote: Lenti-X 293T cells (Takara Bio Inc.) and its derivative ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was then removed using DNase I (Takara, Japan). CircRNA libraries were constructed using the TruSeqTM Stranded Total RNA Sample Preparation Kit from Illumina (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were added to the blocking solution (Rabbit anti-DsRed 1:1000, stock #632496, Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: ... dsRed anti-rabbit (1:500, Clontech, 632496), c- Fos anti-rabbit (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... with TB green premix Ex Taq (Takara, Japan). The primers used were listed in Table S1.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR amplification was typically conducted with ~20 overlapping DNA base primers and the PrimeSTAR HS DNA Polymerase (Clontech) or the Phusion Flash High-Fidelity PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... pTRE3G-HA signal-Flag-GPCRs-P2A-mCherry-reverse-PGK-TetOn3G was made of Tet-ON 3G inducible expression system (Clontech) and PRESTO-Tango GPCR Kit (Kroeze et al. ...
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bioRxiv - Cell Biology 2023Quote: cDNAs of mouse Upf3a and Upf3b were amplified with PrimeSTAR® HS Premix (Takara) from an E14.1 ES cell cDNA library ...
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bioRxiv - Molecular Biology 2023Quote: ... All pHLP plasmids were created using the In-Fusion cloning system (Takara Biosciences) and were confirmed by Wide-seq analysis ...
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bioRxiv - Neuroscience 2023Quote: ... and mCherry (anti-dsRed polyclonal, 1:1000; Clontech, 632496). Sections were then washed in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was converted to double stranded cDNA and amplified using SMART-Seq v4 (Takara, Kusatsu, Shiga, Japan). Full length cDNA was quantified by 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Biochemistry 2023Quote: ... and then reverse transcribed into cDNA using PrimeScript II 1st strand cDNA synthesis kit (Takara Bio). PCRs for AgApiT and FNS I (Yan et al ...
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bioRxiv - Microbiology 2023Quote: ... the CPER products were transfected into HEK293-hACE2/hTMPRSS2 cells by using TransIT-LT1 (Takara, Cat# MIR2305). At one day post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... nine DNA fragments encoding the partial genome of SARS-CoV-2 (hCoV-19/Japan/TY-WK-521/2020) were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A). A linker fragment encoding the hepatitis delta virus ribozyme ...
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bioRxiv - Microbiology 2023Quote: A yeast screen for human cellular interacting proteins of pUL136 was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) and the Mate and Plate Universal Human Library (Clontech) ...
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bioRxiv - Microbiology 2023Quote: ... Yeast dual transformation and yeast mating was performed according to established protocols from Clontech. Yeast prey vectors were isolated from Y187 yeast and subsequently transformed into DH10B E ...
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bioRxiv - Molecular Biology 2023Quote: ... A Lenti-X™ GoStix (Takara) antigen test was used to verify successful lentivirus production.
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bioRxiv - Bioengineering 2023Quote: ... was cloned into the prey vector pPR3-N and co-transformed into yeast strain NMY51 cells together with pDHBⅠ-GhERF105a plasmid according to the Matchmaker user’s manual protocol (Clontech). Yeast transformants were selected by growth on synthetic dropout nutrient medium SD/-Leu/-His/-Ade/-Trp (Clontech ...
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bioRxiv - Molecular Biology 2023Quote: ... coli Stellar™ competent cells (Takara) were combined with 5 µl reaction mix from the cloning step and incubated for 30 min on ice and subsequently heat shocked for 45 sec at 42 °C on a heating block ...
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bioRxiv - Neuroscience 2023Quote: ... pEGFP-C3 (Clontech), pCMV-tdTomato ...
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bioRxiv - Molecular Biology 2023Quote: Protein interactions in vivo in yeast were assayed using the ‘Matchmaker Gold Two-hybrid System’ (Clontech) using the protocols provided by the supplier ...
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bioRxiv - Systems Biology 2023Quote: ... cultures were spiked with 0.5 µM AP21967 (Takara, 635057) or 0.1% ethanol vehicle for 24 hours before cell lysis and biochemical analysis.
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bioRxiv - Systems Biology 2023Quote: ... cultures were spiked with 0.5 µM AP21967 (Takara, 635057) or 0.1% ethanol vehicle ...
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bioRxiv - Microbiology 2023Quote: ... and the Mate and Plate Universal Human Library (Clontech). This yeast two-hybrid universal library was constructed from human cDNA that has been normalized to remove high copy number cDNAs (overrepresented transcripts ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted using TaKaRa MiniBEST Plant RNA Extraction Kit (TaKaRa, 9769) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: Plasmids for targeted gene deletion by homologous recombination were assembled using the In-Fusion® HD Cloning Kit (Takara Bio, Japan). Briefly ...