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Citations for Takara Bio :
1301 - 1350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Genotyping PCR was performed using Prime-STAR GXL DNA Polymerase (Takara) in the presence of 5% DMSO (Kwantes and Wichard 2022 ...
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bioRxiv - Physiology 2024Quote: ... The extracted RNA was reverse-transcribed using the PrimeScript RT Reagent Kit (Takara Bio).
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bioRxiv - Physiology 2024Quote: ... cDNA was synthesized as described above using the PrimeScript RT Reagent Kit (Takara Bio).
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bioRxiv - Physiology 2024Quote: ... Complementary DNA (cDNA) was synthesized using the PrimeScript RT Reagent Kit (Takara Bio, Shiga, Japan).
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bioRxiv - Plant Biology 2024Quote: ... using In-Fusion HD Cloning Kit (Takara Bio). Amino acid substitutions were introduced into pMAL-c5X MAP4K1 or MAP4K2 by site-directed mutagenesis to produce the kinase-dead form of MAP4K1K44N or MAP4K2K44N and the non-phosphorylatable form of MAP4K1K44N/S479A or MAP4K2K44N/S488A ...
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bioRxiv - Synthetic Biology 2024Quote: ... TALON Metal Affinity Resin (Takara) protocols were used ...
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bioRxiv - Synthetic Biology 2024Quote: ... For conditions with the addition of BB homodimerizer (AP20187)(Takara Bio, San Jose, USA), 1μL of 0.5mM BB was diluted in 999μL PBS (HI30 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were collected and AAV6 capsids were isolated using the AAVPro Purification Kit (All Serotypes, Takara Bio, San Jose, USA), as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... and a 500-ng RNA was used for the first-strand cDNA synthesis using PrimeScript™ 1st strand cDNA Synthesis Kit (6110A; TaKaRa, San Jose, California, USA) with oligo(dT ...
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bioRxiv - Plant Biology 2024Quote: ... and cloned into pMDC32 using In-Fusion HD cloning (TaKaRa, San Jose, California, USA) for sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... under the control of the CaMV35S promoter in the binary vector pBI121 (Clontech, USA), was transferred into Agrobacterium tumefaciens strain GV3101 and used to transform tobacco plants by leaf disc method (Horsch et al. ...
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bioRxiv - Plant Biology 2024Quote: ... The potato ST-LS1 IV intron (Eckes et al., 1986) was amplified (Infusion, Takara Bio) using M.SssIN_IV forward and M.SssIC_IV reverse primers (Sup Table 6 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... One microgram of total RNA was reverse transcribed into cDNA using PrimeScript™ RT Master Mix (Cat# RR036A, TaKaRa, Japan). qPCR was conducted with ChamQ SYBR qPCR Master Mix (Cat# Q311-02 ...
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bioRxiv - Physiology 2024Quote: Total RNA (1 µg) was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Cat. # 634875; Takara, Saint-Germain-en-Laye, France). Sequencing was performed on the NextSeq 2000 instrument (Illumina ...
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bioRxiv - Physiology 2024Quote: ... 1μg RNA was transcribed to cDNA using the Takara Primescript RT kit (Takara) as directed by the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... according to the manufacturer’s instructions and reverse-transcribed using a PrimeScript RT reagent Kit (Takara) with the oligo-dT primer ...
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bioRxiv - Plant Biology 2024Quote: ... followed by transformation into Stellar Competent Cells from Takara. For the amplification and Sanger sequencing of single colonies ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from 1 µg of purified total RNA using PrimeScript RT Master Mix (TaKara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... using primers pDAN0869 and pDAN0870 and recombined with pB7-SHHc (Huang et al., 2016b) digested with AvrII using In- Fusion HD cloning (Clontech, Mountain View, CA) to generate pB7-CER-SHHc ...
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bioRxiv - Plant Biology 2024Quote: ... TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara) was used for qPCR analysis in an Mx3000P qPCR machine (Agilent) ...
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bioRxiv - Plant Biology 2024Quote: ... and cDNA synthesis was carried out using the PrimeScriptTM IV 1st strand cDNA Synthesis Mix (DSS Takara Bio India Pvt. Ltd., New Delhi, India). TB Green Premix Ex Taq II (Tli RNase H Plus ...
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bioRxiv - Synthetic Biology 2024Quote: Three separate passages of Lenti-X HEK293T cells (Takara Bio, 632180) grown in DMEM + 10% FBS were seeded at 106 cells per well of a 6-well plate ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments were amplified using PrimeSTAR Max DNA polymerase (Takara Bio. Tech.) and assembled into the vector using In-Fusion Snap Assembly Premix Kit (Takara Bio ...
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bioRxiv - Synthetic Biology 2024Quote: ... subtilis strain F6 was cloned into the vector pBE-S DNA (Takara Bio). The genome of B ...
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bioRxiv - Plant Biology 2024Quote: ... machine using TB Green® Premix EX Taq™ II SYBR Green dye (TaKaRa, www.takarabio.com) following the protocol using 1μl of cDNA per reaction as a template ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting amplicons were pooled and purified by NucleoSpin Gel and PCR Clean-up (TAKARA Bio, Shiga, Japan).
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bioRxiv - Physiology 2024Quote: ... Total RNA was then extracted from the tissues following the guidelines of the RNAiso Plus kit (Takara, Shiga, Japan). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... Viral titer (p24 core antigen content) was determined initially using a LentiX GoStix Plus titration kit (Takara #631280) and subsequently by HIV p24 AlphaLISA detection kit (PerkinElmer # AL291C) ...
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bioRxiv - Genomics 2024Quote: ... 24 mL of supernatant was concentrated 10x using Lenti-X concentrator (Takara Bio) as directed.
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed using the SYBR Green Master MIX Kit (Takara, Japan) according to the manufacturer’s instructions ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... the supernatant was transferred into clean conical tubes and purified by cobalt metal affinity chromatography using approximately 4-6 mL of cobalt resin slurry (Talon Metal Affinity Resin, #635503, Clontech Laboratories, USA) within a gravity-flow column ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The p24 concentrations of viral stocks were determined via a p24 Rapid Titer Kit (Takara). For the LVpp infection assay ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RT-PCR was performed via a SYBR PrimeScript RT-PCR Kit (Takara, Otsu, Shiga, Japan) and qRT-PCR was performed via a TB Green Premix ExTaq II Kit (Takara ...
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bioRxiv - Cell Biology 2024Quote: The SLT construct was inserted using InFusion cloning (Takara) into the pC13N-dCas9-BFP-KRAB plasmid (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... HEK-293 cells were transfected with the lentiviral vector plasmid DNA using Lenti-X Packaging Single Shots (Takara). pCSII-EF-miRFP670v1-hGem(1/110 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by the template DNA digestion with DNase I (Takara Bio). The synthesized guide RNA was purified using the RNA Clean & Concentrator Kit (Zymo Research) ...
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bioRxiv - Cell Biology 2024Quote: ... the constructed vectors were first transfected into the RetroPack PT67 Cell Line (Takara-631510) and transduction to introduce the constructs into the target cells using the procedures previously described (19).
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bioRxiv - Cell Biology 2024Quote: ... Lenti-293T lentiviral packaging cells (female, Clontech, cat. No. 632180), and HEK-293T cells (female ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and qRT-PCR was performed via a TB Green Premix ExTaq II Kit (Takara) on a Bio-Rad CFX-96 system (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... or pEGFP Actin C374S or pEGFP N1 vector (Clontech Catalog #6085-1) and were selected in 5ug/ml Neomycin (Sigma Catalog #A1720 ...
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bioRxiv - Cell Biology 2024Quote: ... and cDNA was synthesized using Primescript RT Master Mix (Takara, RR036B). The cDNAs were then used as templates for quantitative RT-PCR analysis with gene-specific primers ...
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bioRxiv - Cell Biology 2024Quote: ... These fragments were cloned into pHAGE-EF1a-Cre-w41 plasmid using an infusion cloning kit (Takara, 638947). Lentiviral vectors were transfected into the packaging HEK293 cell line ...
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bioRxiv - Cell Biology 2024Quote: HCT116 cells were transfected with 1µg of either pEGFP Actin vector (Clontech Catalog #6116-1) or pEGFP Actin C374S or pEGFP N1 vector (Clontech Catalog #6085-1 ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-GFP antibody (JL-8, Clontech).
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was filtered through an 0.8-μm pore-size filter and subsequently loaded onto a TALON Metal Affinity Resin (Clontech) equilibrated with the lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 4F2hc and mZIP13 or mZIP14 were fused by PCR and In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara). The target cDNA was amplified using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... (treated with and without VA) using Trizol reagent (RNAiso Plus, Takara). One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara) ...
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bioRxiv - Cell Biology 2024Quote: The SLT construct under the control of the EF1α promoter was first inserted using InFusion cloning (Takara) into the pDONOR MCS Rosa26 plasmid (Addgene #37200 ...
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bioRxiv - Cell Biology 2024Quote: ... The target cDNA was amplified using TB Green Premix Ex Taq II (Takara) and the following sense and antisense primers ...