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Citations for Takara Bio :
1251 - 1300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... the reaction mixtures mixed with a 6×loading buffer (Takara, Beijing, China) were loaded into the gel ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 10% tetracycline-free fetal calf serum (FCS) (TaKaRa Bio, 631106), 2 mM L-Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from monocytes using NucleoSpin® RNA kit (Takara) and RNA concentration was measured using NanoDrop Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adapter to the 3’ end of the cDNA by template switching using SMARTScribe (Clontech) as described;86 (ii ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 cells were collected in the center of a 1.5 ml centrifuge tube containing 4.75 μl SMART-seq reaction buffer (Takara) using a BD FACSymphony S6 (BD Bioscience) ...
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bioRxiv - Cancer Biology 2024Quote: RNA was prepared and cDNA was synthesized with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) and ran the Agilent Tapestation to assess cDNA product ...
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bioRxiv - Microbiology 2024Quote: ... The band was extracted and purified with Nucleospin Gel Extraction Kit (Takara). The gel-purified DNA was then further purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was run on a 1% agarose gel at 85 V for 35 min and gel extracted using the Nucleospin Gel Extraction Kit (Takara). We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were homogenized with RNAiso (Takara, Otsu, Japan) and incubated in room temperature for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... using TB Green Premix Ex Taq (TaKaRa) and 0.2 μM of each primer ...
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bioRxiv - Zoology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). The PCR procedure was set as follows ...
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bioRxiv - Immunology 2024Quote: CD8+ T-cells were exposed to the SMARTerTM RACE cDNA Amplification Kit (Clontech/Takara) to identify and annotate ROPN1/B epitope-specific TCRα- and β-chains based on Kunert et al ...
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was conducted using TB Green fluorescence (TaKaRa) on a MasterCycler EP Realplex 4 thermal cycler (Eppendorf ...
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bioRxiv - Immunology 2024Quote: CD8+ T-cells were exposed to the SMARTerTM RACE cDNA Amplification Kit (Clontech/Takara) to identify and annotate ROPN1/B epitope-specific TCRα- and β-chains based on Kunert et al ...
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bioRxiv - Immunology 2024Quote: ... and cloned into the PiggyBac vector (a kind gift from Dr. P.J. French, Erasmus MC, Rotterdam, the Netherlands) using In Fusion cloning kit (Takara). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... Fifteen microliter reactions were analyzed as outlined by the manufacturer (TaKaRa). The relative fold-changes of the transcripts were calculated using the comparative CT(2-ΔΔCT ...
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bioRxiv - Immunology 2024Quote: ... A total of 1 μg of total RNA was reverse-transcribed with random primers using the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: ... In-Fusion enzyme (Takara Bio) was used to insert the gBlocks between the secretion signal and the constant region.
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bioRxiv - Immunology 2024Quote: ... Retronectin-coated (overnight, 12 µg/mL, Takara) Non Tissue Culture flat bottom plates (24 well ...
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bioRxiv - Microbiology 2024Quote: ... pEGFPC1 (Clontech), pEGFP-actin (15) ...
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bioRxiv - Immunology 2024Quote: ... Lenti-X 293T (Takara, cat. #632180), Caco2 ...
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bioRxiv - Immunology 2024Quote: ACE2 cDNA was inserted with In-Fusion cloning kit (Takara Bio) and using the XhoI and BamHI restriction sites into linearized pTRIP-SFFV-CD271-P2A in accordance with the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2024Quote: ... or puromycin (Takara Bio) for 5 minutes then reattached to collagen-coated coverslips ...
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bioRxiv - Cell Biology 2024Quote: ... Puromycin (Takara Bio) or DMSO (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and pMD2-VSV-G to HEK293T cells using the CalPhos Mammalian Transfection Kit (Clontech Laboratories). Viral supernatants were filtered through 0.45 μm low-protein-binding filters ...
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bioRxiv - Molecular Biology 2024Quote: - IgG detector solution V2 (1:2000 dilution, Takara, Cat# T7122A-1).
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bioRxiv - Molecular Biology 2024Quote: ... Complementary DNA (cDNA) was amplificated with SYBR Premix Ex TaqTM (TaKaRa Bio, Otsu, Japan). RT-qPCR was performed using SYBR Green PCR master mix (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... The 13 kb band was purified with gel purification kit (Takara Nucleospin® Gel and PCR Clean-up Midi). Eighty-two base pair single stranded oligos were designed according to the following structure:
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bioRxiv - Microbiology 2024Quote: ... Lentivirus was concentrated using the Lenti-X Concentrator (Takara Bio) according to the manufacturer’s protocol and titrated on HEK293T cells.
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bioRxiv - Cancer Biology 2024Quote: ... Amplification of cDNA product was performed using specific primers with the TB Green® Premix Ex Taq™ II (Takara, RR820B) on a Real-Time PCR detection system (BioRad) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed into complementary DNA (cDNA) using PrimeScript™ RT Reagent Kit (Takara, RR047B). Amplification of cDNA product was performed using specific primers with the TB Green® Premix Ex Taq™ II (Takara ...
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bioRxiv - Microbiology 2024Quote: ... PCR amplifications were performed using high-fidelity PrimeSTAR HS DNA polymerase (Takara Bio) or Q5 high fidelity polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... Conventional PCR was performed with TaKaRa EX Taq kit (TaKaRa) and included 5 µL 10X PCR Buffer (Mg2+ plus) ...
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bioRxiv - Microbiology 2024Quote: Gaps or ambiguous nucleotide positions were corrected by (nested) PCR using TaKaRa EX Taq (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was spun at 5000 rpm for 10 min to remove cell debris and concentrated via Lenti-X as per the instructions (Takara Bio). HIV-Gag-iGFP_JRFL (the following reagent was obtained through the NIH HIV Reagent Program ...
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bioRxiv - Microbiology 2024Quote: ... encoding region at the 3’ terminus of a putative linearized ambi-like virus was done following the protocol of the SMARTer® RACE 5’/3’ Kit (Takara Bio USA, Mountain View, CA, USA), employing a specific primer designed by Primer3-2.3.7 under Geneious (Supplemental Table S2) ...
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bioRxiv - Microbiology 2024Quote: ... The RACE product was cloned using the In-Fusion® HD Cloning Kit (Takara Bio USA) following the producer’s instructions and modifications as described by Botella et al ...
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... RT-qPCR was performed using cDNA to evaluate gene expression with Thermal Cycler Dice (Takara Bio, Inc.) and SYBR Premix Ex Taq II (RR820S ...
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... or ISOGEN (319-90211, NIPPON GENE) and synthesized into cDNA using PrimeScript RT Master Mix (RR036, Takara Bio Inc.). RT-qPCR was performed using cDNA to evaluate gene expression with Thermal Cycler Dice (Takara Bio ...
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bioRxiv - Physiology 2024Quote: ... for qPCR using TB Green Premix Ex Taq (Takara) on a ViiA 7 Real-Time PCR system (Applied Biosystems) ...
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Aromatase in adipose tissue exerts an osteoprotective function in male mice via phosphate regulationbioRxiv - Physiology 2024Quote: ... and SYBR Premix Ex Taq II (RR820S, Takara Bio Inc.). Rpl13a or Gapdh was used as the housekeeping gene ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1×SYBR Green Master Mix buffer (Takara, Otsu, Japan) was used for quantitative RT-PCR and assays were performed on a Roche lightCycler 480 II PCR machine ...
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bioRxiv - Plant Biology 2024Quote: ... using a SYBR Green RT-PCR kit (Takara) and a Roche Light Cycler 480 ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli shuttle vector pBE-S DNA were purchased from Takara Bio ...
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bioRxiv - Plant Biology 2024Quote: ... all gene fragments were cloned into Gateway-compatible variants of pGADT7 and pGBKT7 (Clontech) (50) ...
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bioRxiv - Plant Biology 2024Quote: In-fusion cloning by the In-Fusion HD cloning Kit (TAKARA) was used for plasmid construction ...
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bioRxiv - Plant Biology 2024Quote: ... with the Thermal Cycler Dice Real Time System (Takara). EF1a was used as an internal standard ...
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bioRxiv - Plant Biology 2024Quote: For Total RNA was prepared from seedlings using a NucleoSpin RNA Plant Kit (Takara, Japan) according to the manufacturer’s instructions and reverse-transcribed using a PrimeScript RT reagent Kit (Takara ...
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bioRxiv - Plant Biology 2024Quote: ... Different combinations of bait and prey plasmids were transformed into yeast strain AH109 using PEG/LiCl method (Clontech, USA), and the transformants were selected on synthetic dropout (SD ...
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bioRxiv - Plant Biology 2024Quote: ... ABclonal or mouse monoclonal anti-GFP (JL-8; Clontech, 632381)] with a LumiBest ECL substrate solution kit (Share-bio ...