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Citations for Takara Bio :
1051 - 1100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and genomic DNA was removed using DNase I (Takara). Then ...
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bioRxiv - Immunology 2024Quote: ... 500 ng total RNA or its tissues were then reverse-transcribed using PrimeScript RT (TAKARA) and a mixture of oligo-dT and random hexamer primers ...
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bioRxiv - Immunology 2024Quote: 5’ RACE of lncRNA VILMIR was performed using the SMARTer RACE 5’/3’ Kit (Takara, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... PCR was performed using Titanium Taq (Takara, #639208) with an extension time of 90s for 30 (1st round ...
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bioRxiv - Immunology 2024Quote: ... banked cell pellets were rapidly thawed and processed using the Nucleospin® Blood XL kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and Ex Taq polymerase (Takara) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... pIRES2-AcGFP1 (Clontech Laboratories) and pIRES2-AcGFP1-Myc-IRF415 were used ...
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bioRxiv - Immunology 2024Quote: ... and total RNA was reverse transcribed into cDNA by PrimeScript RT Reagent Kit (Takara) following the established protocol of the kit ...
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bioRxiv - Immunology 2024Quote: ... TALON metal affinity resin (Takara, #635653, 0.5-1:1 v/v ratio) was washed with PBS and added to the supernatant ...
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bioRxiv - Genomics 2024Quote: ... Embryos were fixed and stained using standard Drosophila methods using anti-dsRed (Clontech) and visualized using the ABC-HRP kit (VectorLabs ...
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bioRxiv - Immunology 2024Quote: 2.5×106 Lenti-X 293T cells (Takara; 632180) were seeded into a 10-cm dish and transfected with 6:3:1 ratios of lentiviral plasmid encoding the gene of interest ...
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bioRxiv - Immunology 2024Quote: ... Harvested supernatant was concentrated 100x using the Lenti-X Concentrator (Takara; 631231) and resuspended in complete DMEM and stored at -80°C ...
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bioRxiv - Immunology 2024Quote: ... qPCR was performed using TB Green Premix Ex Taq II (Takara-bio) on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... together with three DNA markers (λ-Hind III digest, Takara Cat. No. 3403; DL15,000 DNA Marker, Takara Cat ...
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bioRxiv - Genomics 2024Quote: ... Lenti-X GoStix Plus (Takara) was used following the manufacturer’s protocol.
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bioRxiv - Genomics 2024Quote: ... together with three DNA markers (λ-Hind III digest, Takara Cat ...
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bioRxiv - Immunology 2024Quote: ... The expression profiles of each gene were conducted by using the SYBR Premix Ex Taq™ (Takara), as previously described ...
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bioRxiv - Microbiology 2024Quote: ... Doxycycline (Clontech) was dissolved in water to a stock concentration of 0.5mg/mL ...
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bioRxiv - Microbiology 2024Quote: ... TaqMan-qPCR was performed using the Premix Ex Taq (Probe qPCR) Kit (TaKaRa, cat# RR390L) in an StepOne Plus Real-Time PCR system (Applied Biosystem ...
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bioRxiv - Microbiology 2024Quote: ... Reverse transcription of RNA was performed using PrimeScriptTM reagent Kit (Perfect Real Time) (Takara Bio Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and L gene open reading frames (ORF) were amplified with PrimeSTAR® Max DNA Polymerase (TAKARA) and ligated between the NcoI and BamHI sites of the pTM1 vector with In-Fusion® Snap Assembly Master Mix (TAKARA ...
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bioRxiv - Microbiology 2024Quote: ... Monoclonal Antibody (JL-8) (Clontech). Anti-mouse IgG was used as a secondary antibody.
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bioRxiv - Microbiology 2024Quote: ... and ligated between the NcoI and BamHI sites of the pTM1 vector with In-Fusion® Snap Assembly Master Mix (TAKARA) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... The HA1 subunit was divided into three fragments for PCR (PrimeSTAR Max DNA Polymerase, Takara Bio), seven Ns and partial sequencing adapters were added in the primer as the barcode ...
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bioRxiv - Immunology 2024Quote: ... genes encoding the variable regions of C7 and C74 heavy chains were cloned into a pMN vector with a human CH1 domain and a C-terminal His-tag using In-Fusion cloning system (Takara Bio #639649). The full light chains of C7 and C74 were cloned into the pMN vector without any purification tag using the same method ...
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bioRxiv - Immunology 2024Quote: ... Cultures were harvested after 7 days and Fabs were purified from culture supernatant using His60 Ni Superflow resin (Takara Bio #635660). Fabs were eluted from the column using a buffer of 50 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... The two PCR products were cloned via In-Fusion HD Cloning (Takara) into the pCAGGS-ARM/GPC plasmid digested with XbaI and KpnI replacing a segment of the wild-type GPC sequence ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using PrimeScript RT-PCR Kit from TaKaRa (#RR037A) (Otsu ...
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bioRxiv - Immunology 2024Quote: ... The full-length CD177 cDNA inserts were sub-cloned into pBMN-I-EGFP retroviral vector digested with Bam HI and EcoR I restriction enzymes using InFusion HD Cloning Kit (Takara Bio USA, Mountain View, Ca) according to the product manual ...
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bioRxiv - Immunology 2024Quote: ... RNA was reverse transcribed and cDNA amplified by in vitro transcription with the TaKaRa SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa, Cat. No. 634889) or a low input method using NEB Next® Ultra RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Immunology 2024Quote: ... equipped with SYBR green detector (TaKaRa Bio, USA) [13] ...
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bioRxiv - Immunology 2024Quote: ... Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step ...
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bioRxiv - Immunology 2024Quote: ... The RNeasy kit and the cDNA Synthesis Kit (Takara, Cat#: 9767, RR047A) was used to extract total RNA and prepare cDNA following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and in-fusion cloned (Takara) into the GPC locus of the mPol1 LCMV S NP/BsmBI cassette digested with BsmBI ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was purified with Terra™ PCR Direct Genotyping Kit (Takara), followed by PCR amplification and Sanger sequencing of specific genomic regions encompassing the target site.
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bioRxiv - Cell Biology 2024Quote: ... 0.2µM of each primer (Table S4) and SYBR® Premix ExTaqTM (Takara) according to the manufacture instructions ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using RNAiso Plus reagent (Takara, 9019) and reverse transcribed into cDNA using the Hifair® AdvanceFast One-step RT-gDNA Digestion SuperMix (Yeasen ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-mouse ACVR1B-HiBiT and HaloTag-mouse ACVR2B-HiBiT lentiviruses were generated in HEK293T cells using Lenti-X Packaging Single Shots (Takara Bio, 631276) and concentrated 20-fold with Lenti Concentrator (OriGene ...
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bioRxiv - Bioengineering 2024Quote: ... TB Green Premix Ex Taq II (12.5 µL; Takara Bio), and nuclease-free water (8.5 µL) ...
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bioRxiv - Bioengineering 2024Quote: ... To synthesize cDNA, total RNA (8 µL, ca. 500 ng) was mixed with 2 µL of PrimeScript™ RT Master Mix (Takara Bio, Kusatsu, Japan) and incubated at 37°C for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilized metal affinity chromatography (IMAC using Talon resin; Takara Bio). The buffer used during the purification process was 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... Lenti-X 293T cells (Cat #632180) were obtained from Takara. All cell lines were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Biochemistry 2024Quote: ... Sub-library specific primers were then used to amplify 1 ul of cDNA template for 24 cycles using Advantage 2 PCR Mix (Takara Bio, Cat. 639206). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was incubated with 5 ml of TALON resin (Takara Bio) for 90 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... HRas V12-C184S] were cloned in the pEGFPC1 vector (Clontech) by restriction digestion-ligation method ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: Flag-RNH1 was sub-cloned into the retroviral vector pMSCVpuro (Clontech) as described previously 21 ...
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bioRxiv - Biochemistry 2024Quote: ... and converted to cDNA using RNA to cDNA EcoDry Premix (Takara, 639547), both according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and media was exchanged for DMEM with 10% Tet-free FBS (Clontech). Supernatants were collected and 0.45 μm filtered at 48 and 72 hours post-transfection and immediately used for infection of MeWo-rtTA target cells in the presence of 8 μg/ml of polybrene (Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: pDual_dsCas9_Venus was prepared by In Fusion cloning (Takara, #638952) using EDCPV vector (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcode sequence of each clone was PCR amplified directly from the cells using Terra PCR Direct Polymerase kit (Takara) for Sanger sequencing using previously described primers (5).