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Citations for Takara Bio :
1101 - 1150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T packaging cells (Clontech #11131D) were cultured in medium consisting of Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Synthetic Biology 2024Quote: ... the viral supernatant was filtered through a 0.45μm PVDF filter and concentrated using the Lenti-X concentrator (Takara #631231) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: WT Human Embryonic Kidney (HEK) 293 cells (ATCC, catalog no. CRL-1573) and HEK293T-LentiX (Takara Biosciences, catalog no. 632180) were cultured in a humidity-controlled incubator under standard culture conditions (37 °C with 5% CO2 ...
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T packaging cells (Clontech #11131D) were cultured in medium consisting of Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 U/uL RNase inhibitor (Clontech, Mountain View, CA, USA), 0.1 mg/mL T7 polymerase (made in house following the protocol from Swartz et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... The human EF1α promoter was sourced from pLVX-Tet3G (Clontech/Takara). Barcodes used for the TUPVs were designed by the Elledge lab34 ...
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bioRxiv - Systems Biology 2024Quote: ... and titers were verified by Lenti-X GoStix Plus (Takara Bio 631280) before storage at -80°C.
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bioRxiv - Systems Biology 2024Quote: ... We used 600 µl of cell lysate for total RNA extraction (Clontech 740955), followed by processing for microarray (for interactome assembly ...
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bioRxiv - Neuroscience 2024Quote: ... GFP (1:1,000, JL-8, Clontech), GABARAP/Atg8a (1:2,000 ...
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bioRxiv - Physiology 2024Quote: ... qPCR was performed with Thermal Cycler Dice Real-time System II (Takara Bio, Shiga, Japan) and Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... Both PHI-1 ORF and CESAs ORFs were amplified from first-strand DNA with PrimeScriptRTase (TaKaRa) using primers indicated in Supplemental information 4 ...
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bioRxiv - Plant Biology 2024Quote: ... with the SYBER® Premix Ex Taq TM II kit (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... coli and verified the expression cassette by sequencing and repeated again this yeast two hybrid assay by using the verified plasmid DNA instead of the corresponding PCR fragment inserts and linear vectors for mbSUS interaction tests as described “mbSUS tests protocol” (Clontech).
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bioRxiv - Plant Biology 2024Quote: ... First strands were synthesized using PrimeScript® 1st Strand cDNA Synthesis Kit (TaKaRa). RT-PCR was used to test for the presence of transcripts ...
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bioRxiv - Synthetic Biology 2024Quote: ... was used and the synthesized mRNAs were purified with NucleoSpin RNA Plus (Takara). The concentration of mRNA was measured by Nano Drop One (Thermo Fisher scientific ...
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bioRxiv - Neuroscience 2024Quote: ... AAV2/9 particles were purified using the AAV Purification kit (Takara, Japan). The AAV solution was concentrated to the optimal volume by centrifugation using an Amicon Ultra-4 100k centrifugal filter unit (Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... and then transformed in Escherichia coli HST08 competent cells (Takara). The sequence integrity of inserts carried by transformants were verified by Sanger sequencing ...
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bioRxiv - Synthetic Biology 2024Quote: Total RNA was extracted using RNAiso plus (Takara) 48-72h after transient transfection or lentivirus infection ...
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bioRxiv - Synthetic Biology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). Amplified products were sequenced and verified after cloning into a pJET1.2-T vector through CloneJET PCR Cloning Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 100 ng pEGFP-N1 (Clontech). (4 ...
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bioRxiv - Molecular Biology 2024Quote: ... and cDNA was synthesized using the PrimeScript RT reagent kit with gDNA eraser (TaKaRa). A random hexamer was used for cDNA synthesis ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized at 44°C for 15 min using the PrimeScript RT reagent kit with gDNA eraser (TaKaRa) and the y300 PAT universal C10 primer ...
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bioRxiv - Molecular Biology 2024Quote: ... qRT–PCR was performed using SYBR Premix Ex TaqII (Tli RNaseH Plus) and the Thermal Cycler Dice Real Time System (TaKaRa). The data were normalized using 18S rRNA as a reference ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... Lenti-X 293T cells (Takara) were cultured in complete DMEM supplemented with 10% fetal bovine serum and 1% antibiotics (pen/strep).
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bioRxiv - Synthetic Biology 2024Quote: ... has been acquired from Takara Bio.
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bioRxiv - Synthetic Biology 2024Quote: ... The mixture (1 μL) was used as the template in 10 μL of PrimeSTAR GXL DNA Polymerase (Takara Bio Inc., Japan) reaction ...
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bioRxiv - Synthetic Biology 2024Quote: Cells were treated with Doxycycline (Clontech), Shield1 (MedCehmExpress ...
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bioRxiv - Synthetic Biology 2024Quote: ... and spectinomycin-resistant gene with lacI–spac promoter unit were amplified through polymerase chain reaction (PCR) using PrimeSTAR DNA polymerase (TaKaRa, Shiga, Japan), along with appropriate primer sets (namely F1/R2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Gal4-NGN2 plasmid was constructed using Gibson Assembly with In-Fusion® Snap Assembly Master Mix (Takara Bio #638947) and following recommended manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... At 48 hours the viral supernatant was filtered through a 0.45μm PVDF filter and concentrated using the Lenti-X concentrator (Takara, #631231) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T cells (Takara Bio #632180) were seeded at approximately 7e5 cells/well in a 6-well plate to yield ∼80% confluency the following day ...
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T packaging cells (Clontech #11131D) were cultured in medium consisting of Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Synthetic Biology 2024Quote: ... Polymerase chain reaction (PCR) was performed using Primer STAR Max DNA Polymerase (Takara) to amplify the target gene ...
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bioRxiv - Neuroscience 2024Quote: ... The preserved HEK medium was then spun down with 12.5 ml of lenti-X concentrator (Takara Bio), in an effort to capture the two distinct lentiviruses ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries of cDNA were then generated using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara). The resulting cDNA libraries were then quantified by the High Sensitivity DNA assay ...
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bioRxiv - Neuroscience 2024Quote: ... and re-plated at a concentration of 2.5×105 cells/cm2 onto plates coated with 4 μg/mL iMatrix-511 (Takara Bio) in N2B27 medium supplemented with 10 μg/mL BDNF and 10 µg/mL Rock Inhibitor Y-27632 ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit Anti-dsRed (Takara 632496, 1:1000); Goat Anti-Calb2 (Swant CG1 ...
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bioRxiv - Molecular Biology 2024Quote: ... After RNA Reverse transcription (Prime Script RT reagent, Takara), cDNA levels were measured by qPCR Fast SYBR™ Green Master Mix (Ref ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit anti-dsRed (Takara 632496, 1:1,000–1:2,000), and Chicken anti-GFP (Aves GFP-1020 ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA synthesis was performed using a one-step PrimeScript RT-PCR kit (Takara Bio, Cat. RR057B, Japan) as per the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... using a random primer DNA labeling kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... a two-round PCR strategy was required to amplify the ORF using a high-fidelity PrimeStar ® DNA polymerase (Takara Europe, Saint-Germain-en-Laye, France). The first round of the nested PCR was carried out using the above cDNA as the template with primers designed in the 5’- and 3’-untranslated regions (UTR ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were processed for DNA library preparation (ThruPLEX DNA-seq Kit, Takara Bio), cleaned up with AMpure XP beads (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was filtered using a 0.25 µm syringe filter and applied to Talon metal affinity resin (Takara) in 50mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... The digested DNA was self-ligated using T4 DNA ligase (Takara, Japan). The ligated product was precipitated using 3M sodium acetate and 100% ethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1x M buffer containing 160 units of Nhe I (TaKaRa) overnight at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.025 μL recombinant RNase inhibitor (Takara, 2313B), 0.04 μL reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.02 μL recombinant RNase inhibitor (Takara, 2313B) and 2.21 μL nuclease-free water per reaction (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-STEM121 (Takara, Y40410, 1:250), goat anti-ChAT (Sigma-Aldrich ...