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Citations for Takara Bio :
1151 - 1200 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: The SLT construct was inserted using InFusion cloning (Takara) into the pC13N-dCas9-BFP-KRAB plasmid (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... HEK-293 cells were transfected with the lentiviral vector plasmid DNA using Lenti-X Packaging Single Shots (Takara). pCSII-EF-miRFP670v1-hGem(1/110 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by the template DNA digestion with DNase I (Takara Bio). The synthesized guide RNA was purified using the RNA Clean & Concentrator Kit (Zymo Research) ...
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bioRxiv - Cell Biology 2024Quote: ... the constructed vectors were first transfected into the RetroPack PT67 Cell Line (Takara-631510) and transduction to introduce the constructs into the target cells using the procedures previously described (19).
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bioRxiv - Cell Biology 2024Quote: ... Lenti-293T lentiviral packaging cells (female, Clontech, cat. No. 632180), and HEK-293T cells (female ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and qRT-PCR was performed via a TB Green Premix ExTaq II Kit (Takara) on a Bio-Rad CFX-96 system (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... or pEGFP Actin C374S or pEGFP N1 vector (Clontech Catalog #6085-1) and were selected in 5ug/ml Neomycin (Sigma Catalog #A1720 ...
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bioRxiv - Cell Biology 2024Quote: ... and cDNA was synthesized using Primescript RT Master Mix (Takara, RR036B). The cDNAs were then used as templates for quantitative RT-PCR analysis with gene-specific primers ...
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bioRxiv - Cell Biology 2024Quote: ... These fragments were cloned into pHAGE-EF1a-Cre-w41 plasmid using an infusion cloning kit (Takara, 638947). Lentiviral vectors were transfected into the packaging HEK293 cell line ...
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bioRxiv - Cell Biology 2024Quote: HCT116 cells were transfected with 1µg of either pEGFP Actin vector (Clontech Catalog #6116-1) or pEGFP Actin C374S or pEGFP N1 vector (Clontech Catalog #6085-1 ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-GFP antibody (JL-8, Clontech).
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was filtered through an 0.8-μm pore-size filter and subsequently loaded onto a TALON Metal Affinity Resin (Clontech) equilibrated with the lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 4F2hc and mZIP13 or mZIP14 were fused by PCR and In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara). The target cDNA was amplified using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... (treated with and without VA) using Trizol reagent (RNAiso Plus, Takara). One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara) ...
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bioRxiv - Cell Biology 2024Quote: The SLT construct under the control of the EF1α promoter was first inserted using InFusion cloning (Takara) into the pDONOR MCS Rosa26 plasmid (Addgene #37200 ...
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bioRxiv - Cell Biology 2024Quote: ... The target cDNA was amplified using TB Green Premix Ex Taq II (Takara) and the following sense and antisense primers ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara). The target cDNA was amplified using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... Lenti-X™ p24 Rapid Titer Kit (Takara Bio) was used to measure the titer of collected SARS-Cov2-PP by ELISA (Enzyme-Linked Immunosorbent Assay) ...
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bioRxiv - Cell Biology 2024Quote: ... concentrated using Lenti-X concentrator kit (Takara bio, Japan). Lenti-X™ p24 Rapid Titer Kit (Takara Bio ...
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bioRxiv - Cell Biology 2024Quote: ... by using In-Fusion cloning kit (TaKaRa). For pAAVS1-Nst-MCS (Addgene #80487) ...
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bioRxiv - Cell Biology 2024Quote: ... GS linker human IgG1 FC region from pcDNA3-sACE2v2-Fc (IgG1) and PGK promoter puromycin resistance region cloned into lenti CAG-FLAG-dCas9-VPR using infusion cloning kit (Takara, 638947). After packaging the lentivirus ...
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bioRxiv - Cell Biology 2024Quote: ... Viral particles were precipitated by adding Lenti-X Concentrator (Takara, #631232) according to the manufacturer’s instructions
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bioRxiv - Cell Biology 2024Quote: Cells were collected in Trizol (RNAiso Plus Takara) and RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... we amplified EF1a promoter region from pEP4 E02S CK2M EN2L by PCR and cloned into pHR_SFFV_LaG17_synNotch_TetRVP64 using infusion cloning kit (Takara, 638947). After packaging the lentivirus ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Extracted total RNAs of both species were purified using DNase I (TaKaRa Bio). Total RNA of G ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR amplification of the sequences was performed using Ex Premier DNA Polymerase (TaKaRa Bio).
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bioRxiv - Evolutionary Biology 2024Quote: All TRIM5α constructs were generated with a C-terminal HA epitope tag and cloned into pQCXIP (Takara) to allow CMV-driven TRIM5α expression and selection of transductants via the IRES-puromycin resistance cassette ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RACE was performed using Advantage 2 Polymerase Mix (Clontech Laboratories). The obtained 5□- and 3□-RACE products were purified using a QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The extracted RNA was purified using DNase □ (TaKaRa Bio, Shiga, Japan). cDNA was synthesized using a SMARTer RACE cDNA Amplification Kit (Clontech Laboratories ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Primestar HS polymerase (Takara) was used to minimize errors during full-plasmid amplification (98°C 10” ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cDNA was synthesized using a SMARTer RACE cDNA Amplification Kit (Clontech Laboratories, Mountain View, CA, USA). Gene-specific primers were designed using Primer3 Plus (Untergasser et al. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli DH5α Electro-Cells (Takara Bio, Japan, 9027) to construct the pUA66-mCherry plasmid and its derivatives ...
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bioRxiv - Immunology 2024Quote: ... scFv_myc_hCD8hCD28_hCD3ζ CAR subcloned into a pSAMEN vector backbone using the supernatant of stably retrovirus producing PG13 cells and Retronectin (Takara Bio, Kusatsu, Shiga, Japan) coated culture plates as per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR amplification was performed using PrimeSTAR GXL DNA polymerase (TaKaRa). The resulting barcoded PCR products were pooled for SMRTbell library construction and sequenced on the Sequel platform.
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bioRxiv - Genomics 2024Quote: ... employing the Fruit-mate™ and NucleoSpin™ RNA Plant kits (Takara Bio Inc., Shiga, Japan), in accordance with the manufacturers’ instructions ...
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bioRxiv - Genomics 2024Quote: ... A genetic screen was performed using the Matchmaker Gold Y2H system (Clontech) to generate cnd1 mutations that disrupt the Cnd1-Pmc4 interaction without affecting the condensin complex ...
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bioRxiv - Genomics 2024Quote: ... The pGADT7 derivatives that express GAL4-AD fused to either Pmc4 or Cnd2 (GAL4-AD-Pmc4 or GAL4-AD-Cnd2) in addition to the LEU2 marker gene were independently transformed into the Mat a Y2HGold budding yeast strain (Clontech). After crossing Mat α strain with Mat a strain on YEA plates ...
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bioRxiv - Genomics 2024Quote: ... the mouse monoclonal antibodies [anti-Myc (Takara Bio), anti-Pk (BioRad) ...
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bioRxiv - Immunology 2024Quote: ... STAT1 cDNA was PCR-amplified from Addgene #8691 (a gift from Jim Darnell (Horvath et al, 1995)) and sub-cloned into pLVX-IRES-Puro (Takara) via the BamHI and NotI sites ...
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bioRxiv - Immunology 2024Quote: ... SMART MMLV reverse transcriptase (639524) was purchased from Clontech, Takara Bio ...
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bioRxiv - Immunology 2024Quote: ... Random primer (Hexadeoxyribonucleotide mixture, pd(N)6) (3801) was purchased from Takara Bio ...
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bioRxiv - Pathology 2024Quote: ... First-strand cDNA was synthesized using a PrimeScript RT Master Mix (RR036, Takara). Polymerase chain reactions were carried out using a SsoFast EvaGreen Supermixes (Bio-Rad ...
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bioRxiv - Pathology 2024Quote: ... DsRed (Clontech, 632496, 1:4000), NEK8 (gift from D ...
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bioRxiv - Plant Biology 2024Quote: ... and expression was quantified using the TB Green® Premix Ex Taq™ II (Takara). Primer sequences used for qPCR are listed in Supplemental Table S2.
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bioRxiv - Genomics 2024Quote: ... Cellular RNAs were digested with 50 µg RNase A (Takara Bio) in PEMBAL buffer at 37°C for 2 hours ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries were prepared from isolated RNA using two different kits: Takara SMART-Seq v4 3′ DE (Takara) and Lexogen QuantSeq 3′ (Lexogen ...
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bioRxiv - Genomics 2024Quote: ... Anhydrotetracycline (Takara Bio, 631310) was then added to the growth chambers and the media reservoir to a final concentration of 250 ng/ml ...
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bioRxiv - Genomics 2024Quote: ... the RNA samples were reverse-transcribed to cDNA using the SMART-Seq HT Kit (Takara Bio USA, CA, USA) following the manufacturer’s guidelines ...
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bioRxiv - Genomics 2024Quote: ... using random primer DNA labeling kit (Takara). A total of 100 ng FISH probes were suspended with hybridization buffer (50% formamide ...