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Citations for Takara Bio :
1151 - 1200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Primers oAS344 and oAS345 were then both used to amplify the cDNA using CloneAmp PCR Master Mix (Takara 639298) according to manufacturing protocol ...
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bioRxiv - Cell Biology 2024Quote: ... was subcloned into the lentiviral expression vector pLVX-M-puro (Takara 632164). For CRISPR-KO experiments ...
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bioRxiv - Cell Biology 2024Quote: ... Pairs of pGBKT7 and pGADT7 plasmid were co-transformed into the yeast strain AH109 following the MatchmakerTM GAL4 Two-Hybrid System instructions (Clontech). Primary transformants were selected on synthetic drop-out (SD ...
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bioRxiv - Cancer Biology 2024Quote: ... the total RNA of fibroblasts was isolated with RNAiso reagent (Takara Bio, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted by RNAiso Plus (Takara, No.9109) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and amplified using ExTaqII SYBR Premix (Takara Bio Inc.) on an Aria Mx real-time thermocycler (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... following the manufacturer’s instructions (Takara Bio). This approach resulted in the identification of both SmFADS ends ...
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bioRxiv - Immunology 2024Quote: ... and 10 μl Premix Ex Taq (TaKaRa, Shiga, Japan). Amplification conditions were as follows ...
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bioRxiv - Biochemistry 2024Quote: ... The Cys/Ser-substituted BPTI mutant (BPTI all-Cys/Ser) was constructed using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Japan). BPTI all-Cys/Ser was overexpressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... TMPRSS2 ectodomain constructs were further mutated by In-Fusion Assembly (Takara Bio). All TMPRSS2 sequences are listed in Table S1.
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bioRxiv - Bioengineering 2024Quote: ... VLP were quantified by a LFA assay for HIV p24 (GoStix Plus, Takara). In this case the number of virions per volume is calculated by the approximate stoichiometry of p24 in VLP (∼2000/virion) ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was treated with DpnI (TaKaRa Bio) at 37 °C for 1 h and transformed into Escherichia coli JM109 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined by bicinchoninic acid assay using bovine serum albumin (TaKaRa Bio) as the reference standard ...
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bioRxiv - Biochemistry 2024Quote: ... about ∼5 μg of bacmid were transfected using 5 μL of TransIT®-Insect transfection reagent (Takara Bio Inc.). 5 days after initial transfection ...
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bioRxiv - Biochemistry 2024Quote: ... This was followed by ligation of the guide RNA to the ligation oligo using the DNA Ligation Kit (Takara). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... TMPRSS2 constructs were purified using TALON metal affinity resin (Takara Bio) and washed with 100 column volumes of 5 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... and RNA extraction kit were bought from Takara (Dalian, China). Ultrapure water was employed throughout the work ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated with TALON resin (Clontech) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... The sequence of human histone H2A(K15C-129) was cloned into the pCold-Trigger factor (TF) vector (Takara Bio) with a SUMO coding sequence inserted to generate His6–TF–SUMO–H2A(K15C-129 ...
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bioRxiv - Biochemistry 2024Quote: ... The RNA was quantified and 1.5 μg of RNA was used to prepare cDNA using PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio) and random hexamer primer ...
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bioRxiv - Biochemistry 2024Quote: ... The other 2A proteins were separated from the lysate using immobilized metal affinity chromatography with either TALON beads (Clontech) or chelating Sepharose beads charged with NiCl2 (Cytiva) ...
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bioRxiv - Microbiology 2024Quote: ... M4B and M4 midgut regions were harvested in RNAiso Plus (Takara Bio). This resulted in 24 samples (Apo and Sym insects × 4 timepoints × 3 midgut regions) ...
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bioRxiv - Biochemistry 2024Quote: Target RNA was labeled at the 3′ end using Poly(A) Polymerase (Takara) and [32P]cordycepin-5′-triphosphate ...
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bioRxiv - Biochemistry 2024Quote: ... unreacted ligation oligo was dephosphorylated using alkaline phosphatase (Calf intestine) (Takara) and formamide dye [10 mM EDTA ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was incubated with 35μl of Talon metal affinity resin (TaKaRa) at 4°C for 3 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Lenti-X 293T (Cat# 632180, Takara Bio) cells were seeded on 6-well plates (∼400,000 cells/well ...
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bioRxiv - Biochemistry 2024Quote: ... GFP (mouse, Clontech cat# 632380; 1:2,000); FLAG (mouse ...
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bioRxiv - Cancer Biology 2024Quote: ... and pLVX-E1Fα-IRES-mCherry-Puro (Takara Bio) to perform the experiments associated with its ectopic expression ...
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bioRxiv - Cell Biology 2024Quote: ... iSLK and iSLK-BAC16 cell line derivatives were generated using 2nd generation lentivirus as follows: 1×106 Lenti-X 293T cells (Clonetech/Takara via the UC Berkeley Tissue Culture Facility ...
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bioRxiv - Cell Biology 2024Quote: ... using InFusion cloning (Takara Bio). TFIIB-2xStrep was then amplified and subcloned into the Sfi1 site of a sleeping beauty plasmid pSBbi-GP (Addgene #60511 ...
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bioRxiv - Cancer Biology 2024Quote: ... we generated lentivirus using Lenti-XTM Tet-OneTM system (Takara). To induce overexpression of YTHDC1 wild-type and mutant ...
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bioRxiv - Cell Biology 2024Quote: ... mCherry-RILPL1 was created with IN-FUSION (Takara) from the mCherry-Climp63 vector (Addgene#136293 ...
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bioRxiv - Cancer Biology 2024Quote: ... The C119S and C160S DR5 mutants were subsequently cloned into the EcoRI and BamHI sites of pRetroX-TetOne- Puro vector (Clontech, Mountain View, CA, USA). Mutation of the DR5 C94 was produced in the DR5 C81S construct to produce the DR5 C81S/C94S using QuikChange mutagenesis and the following primers ...
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bioRxiv - Cancer Biology 2024Quote: ... and Cla I sites 5′ to the BamH I site (Clontech, Mountain View, CA, USA).
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bioRxiv - Cancer Biology 2024Quote: ... and real-time qPCR experiments were conducted using the SYBR fluorescent dye kit (TaKaRa, Japan). The primers used in this procedure included ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription of total RNA was performed using a two-step kit (TaKaRa, Japan), and real-time qPCR experiments were conducted using the SYBR fluorescent dye kit (TaKaRa ...
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bioRxiv - Cancer Biology 2024Quote: ... the RNA Isolation Kit (RNAiso Plus, Takara, Japan) was employed ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed with PrimeScriptTM RT reagent Kit (Takara #RR037B), and quantitative assays were performed in triplicate using SYBR Premix Ex TaqTM II (Takara #RR820A ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... or media with Tet System Approved FBS (Takara Bio cat# 631107; Mountain View, USA) with and without doxycycline hyclate for 72 h and lysed in RIPA buffer (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using SMART-Seq Stranded Kit (Takara Bio). The quality ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative assays were performed in triplicate using SYBR Premix Ex TaqTM II (Takara #RR820A) in Bio-Rad iQ5 system ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was conducted using SYBR Green PCR Master Mix (TaKaRa) on a Fast-Real-Time PCR System ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed using TB Green Premix Ex Taq ™ II (Tli RNaseH Plus) (Takara) with qTOWER3 (Jena) ...
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bioRxiv - Cancer Biology 2024Quote: ... The cDNA synthesis and gDNA erase protocol were performed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara). Quantitative real-time RT-PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse-transcribed using a cDNA synthesis kit (TaKaRa Bio, Kusatsu, Japan). RT-qPCR was conducted using SYBR Green PCR Master Mix (TaKaRa ...
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bioRxiv - Cancer Biology 2024Quote: ... and Shield-1 (TaKaRa, Cat#632189, 1μM) for a period of 4 hours in charcoal stripped FBS containing medium ...
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bioRxiv - Cancer Biology 2024Quote: ... A431 epithelial carcinoma cells (ATCC; Manassas, VA) and GP2-293 cells (Takara Bio USA; San Jose, CA) were cultured in high glucose DME ...
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bioRxiv - Cancer Biology 2024Quote: ... 4T1 cells were transduced with a luciferase cDNA cloned into the pQCXIN retroviral expression vector (Takara Bio USA; San Jose, CA), selected with 0.5 mg/ml G418 ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR products were subsequently cloned to the pMD19-T vector (TaKaRa) for Sanger sequencing.
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bioRxiv - Cancer Biology 2024Quote: We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC-97L cells using the SMARTer™ RACE cDNA Amplification Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol ...