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Citations for Takara Bio :
1001 - 1050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... [50] The ssDNA scaffold was initially prepared using Guide-it Long ssDNA Production System v2 kit (Takara Bio, 632666) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell free extracts in a single step using immobilized affinity chromatography (IMAC) with Talon resin (ClonTech) and dialyzed overnight into 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... coli DH5α (Takara Bio Inc.) to obtain the plasmid for the linker-inserted variant.
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bioRxiv - Biochemistry 2024Quote: ... Sorted populations were collected and genomic DNA was isolated using DNAiso Reagent (Takara, 9770A). sgRNA cassettes were amplified by PCR and subjected to Next-generation sequencing (NGS ...
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bioRxiv - Biochemistry 2024Quote: ... and XcSGL (KEGG locus tag, XCC2207) were amplified by PCR chain reaction using KOD plus (TOYOBO, Osaka, Japan) or PrimeSTAR Max (Takara Bio, Shiga, Japan) for the DNA polymerase and the primer pairs ...
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bioRxiv - Biochemistry 2024Quote: ... pLysS or Rosetta pLysS cells and overexpressed proteins were purified using immobilized metal-ion affinity chromatography (IMAC) with TALON resin (Clontech) (Abad et al ...
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bioRxiv - Biochemistry 2024Quote: ... Primers were designed using a PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The codons S85 and N86 were designed as the recognition sequence (TTCGAA) of BspT104 I (Takara Bio Inc., Shiga, Japan) for further modification of the linker insertion ...
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bioRxiv - Bioengineering 2024Quote: ... The resulting cDNA was utilized for analysis using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara Bio). Quantitative PCR was performed using Applied Biosystems QuantStudio™ 5 (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... DNA sequence of EB3 were obtained from pETMz mGFP-EB3 (gift from Thomas Surrey lab) were cloned by infusion (Takara Bio) into the GCN4_v4 plasmids using the primer (EB3_LZ_for ...
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bioRxiv - Bioengineering 2024Quote: ... MSCs and chondrocytes were fluorescently labeled using mitochondrial or cytoplasm targeted lentiviruses driven by an EF1α promoter for co-cultures (Takara Bio USA, Inc.). Mitochondria were labeled with GFP or mCherry (0017VCT ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid was used to generate N-terminal-truncated MGME1 (ΔN-MGME1; residues 95 to 344) by means of In-Fusion Cloning (Takara Bio). Constructs expressing the MGME1 variants used in this study were generated by site-directed mutagenesis using the pSol-His8-SUMO-MGME1 plasmid as template ...
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bioRxiv - Bioengineering 2024Quote: ... The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina) ...
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bioRxiv - Bioengineering 2024Quote: ... GFP was detected with a monoclonal mouse anti-GFP antibody (JL-8, Takara Bio USA, San Jose, CA) in Western blot analyses.
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bioRxiv - Bioengineering 2024Quote: ... The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit, Takara, 639650), and the PCR-derived regions of the resulting plasmids were confirmed by sequencing.
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bioRxiv - Biochemistry 2024Quote: ... DNaseI-treated total RNA (4 µg) was reverse transcribed using Primescript reverse transcriptase (Takara Bio) and random hexamers (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Lenti-XTM 293T (Takara, 632810) cells were transfected GFP-LC3 plasmid ...
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bioRxiv - Biochemistry 2024Quote: The Upf1-HD-His6 wild-type protein was first purified on a TALONTM resin (Clontech) equilibrated with buffer A200 ...
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bioRxiv - Biochemistry 2024Quote: ... The linear PCR product was then re-circularized with In-Fusion Cloning Master Mix (Takara Bio, 638948) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... and the remaining RNA isolation was done using the NucleoSpin RNA II Kit (Clontech Laboratories, Palo Alto, CA; 740955.50). 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... we employed the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) or WST-1 (Water-Soluble Tetrazolium 1) assay from Takara, Japan ...
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bioRxiv - Cancer Biology 2024Quote: 1 x 106 ACKP cells were transfected with 3 µg pE2F-TA-luc plasmid (Takara) and 0.3 µg renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR fragments containing pLib vector backbone (Clontech) and EF1A promoter ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used: anti-GFP (JL-8; 1:20, Clontech, Saint-Germain-en-Laye, France), anti-PHB1 (EP2803Y ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP (JL-8, 1:3000, Clontech, Saint-Germain-en-Laye, France) and anti-Actin (AC74 ...
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bioRxiv - Cell Biology 2024Quote: ... GenTLE Precipitation Carrier (Takara). These purified ribosome footprint RNAs were ligated with linker oligonucleotides containing an inner index sequence and a unique molecular identifier (UMI) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated with Trizol (Thermo) and cDNA was synthesized with Prime Script RT Reagent Kit (Takara). Quantitative PCR was performed with a SYBR Green PCR kit (Toyobo ...
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bioRxiv - Cell Biology 2024Quote: ... ovaries were collected in 1ml of TRIzol (Takara - 9108). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase 8 reporter was established by replacing the DEVD amino acid sequence with XEXD in pLenti_CMV_GFP_Blast_FLIP_GFP_DEVD plasmid through the In-fusion Snap assembly master mix (Takara Bio) using the following oligos forward ...
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bioRxiv - Bioengineering 2024Quote: ... lentivirus targeting mCherry to the cell membrane (Takara, 0026VCT, rLV.EF1.mCherry-Mem-9) was used according to manufactures recommendation ...
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bioRxiv - Bioengineering 2024Quote: ... Appropriate colonies were grown and the recombinant bacmid DNA was extracted using the NucleoBond Xtra Midi plasmid purification kit (TAKARA). The fifth instar silkworm larvae ...
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bioRxiv - Bioengineering 2024Quote: TB Green Advantage qPCR premix (Takara Bio, Cat. No. 639676) was used for the amplification of osteogenic and chondrogenic markers ...
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bioRxiv - Bioengineering 2024Quote: ... All proteins were purified with a Talon metal affinity resin (Takara Bio USA, Mountain View, CA, USA) and dialyzed against pyrogen-free PBS ...
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bioRxiv - Bioengineering 2024Quote: AAV-vector plasmid and AAV-crTSG were used for AAV9 production and purification using Lenti-X 293 T cells (Takara Bio). Each set of Lenti-X 293 T cells (6 150mm-plates ...
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bioRxiv - Bioengineering 2024Quote: ... The plasmid pmCherry was generated by fusing the lacI-Ptrc inducible system and mCherry into the vector pBBR1MCS-5 using In-Fusion® Snap Assembly Master Mix (Takara Bio). All plasmids were extracted using QIAprep® Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Bioengineering 2024Quote: ... and reverse-transcribed into cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio). The resulting cDNA was utilized for analysis using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Bioengineering 2024Quote: ... Viral genome number was quantified by real-time PCR with AAVpro Titration Kit Ver.2 (Takara, 6233) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2024Quote: ... AAV was purified with the AAVpro Purification Kit (Takara, 6666) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2024Quote: ... filtered with a 0.45μm filter to remove cellular debris and concentrated (100x) using Lenti-X concentrator (Takara, San Jose, CA). The resulting particles were resuspended in DMEM with 10% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... mixed with one-tenth of the volume of Lenti-X Concentrator (Takara Bio), and incubated at 4 °C for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... The cleared supernatant was then mixed with Lenti-X Concentrator (Takara Bio) and incubated for 24 h at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... was cotransfected into HEK293T cells (Takara) with the packaging plasmid psPAX2 (Addgene ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... cDNA was generated using SMART-seq v4 Ultra Low Input RNA Kit (Takara Bio) and the final sequencing library was made using the Nextera XT Kit (Illumina ...
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bioRxiv - Bioengineering 2024Quote: ... SEAP production was analyzed by collecting medium and running a chemiluminescent assay on samples (Takara Bio) using a Tecan Infinite M1000 Pro plate reader.
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bioRxiv - Bioengineering 2024Quote: cDNA and the plasmid library were amplifed using CloneAmp PCR Master Mix (Takara 639298) and primers for amplicon sequencing (Supplemtary Note 23) ...
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bioRxiv - Bioengineering 2024Quote: ... and the receptor- or payload-encoding viral expression plasmids (2.0 μg) into Lx293T cells (Clontech) in 6-well plates with Lipofectamine 3000 (Thermo Fisher L3000001) ...
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bioRxiv - Bioengineering 2024Quote: Primers oAS344 and oAS345 were then both used to amplify the cDNA using CloneAmp PCR Master Mix (Takara 639298) according to manufacturing protocol ...
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bioRxiv - Cell Biology 2024Quote: ... was subcloned into the lentiviral expression vector pLVX-M-puro (Takara 632164). For CRISPR-KO experiments ...
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bioRxiv - Cell Biology 2024Quote: ... Pairs of pGBKT7 and pGADT7 plasmid were co-transformed into the yeast strain AH109 following the MatchmakerTM GAL4 Two-Hybrid System instructions (Clontech). Primary transformants were selected on synthetic drop-out (SD ...
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bioRxiv - Cancer Biology 2024Quote: ... the total RNA of fibroblasts was isolated with RNAiso reagent (Takara Bio, Japan) according to the manufacturer’s instructions ...