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Citations for Takara Bio :
1001 - 1050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... were digested and cloned using an Infusion master mix (Takara) upstream of the luciferase gene ...
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bioRxiv - Genomics 2024Quote: ... Library preparation and HiFi genome sequencing were performed by Takara Bio (Shiga ...
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bioRxiv - Genomics 2024Quote: ... except that an alternative oligo-dT primer from the SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc.), which also included a random 10mer sequence as a unique molecule identifier (UMI ...
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bioRxiv - Genetics 2024Quote: ... PCR was conducted as described earlier (section: RNAi-mediated knockdown of Pmtra-2) but using the proofreading PrimeSTAR HS DNA Polymerase (Takara Bio, Shiga, Japan) and Ex Taq DNA Polymerase (Takara Bio ...
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bioRxiv - Genetics 2024Quote: ... and Ex Taq DNA Polymerase (Takara Bio, Shiga, Japan) respectively in the 1st- and 2nd-round PCR ...
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bioRxiv - Genomics 2024Quote: ... The PCR reaction mix consisted of 1.25 PrimeSTAR GXL DNA Polymerase (Takara Bio USA, San Jose, CA), 1× Buffer ...
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bioRxiv - Genomics 2024Quote: Illumina sequencing libraries are prepared by incorporating 1-10 ng of cfDNA with the ThruPLEX® Plasma-seq Kit by Takara Bio ...
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bioRxiv - Genomics 2024Quote: ... followed by an Amicon filter (MilliporeSigma, Burlington, MA) buffer exchange (Vaillancourt et al., 2019) or Takara NucleoBond HMW DNA kit (Takara, Kusatsu, Shiga, Japan). Genome assembly used hifiasm v0.16.1-r375 (Cheng et al ...
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bioRxiv - Genomics 2024Quote: ... Lysates were incubated with His60 Ni Superflow resin (Takara Bio) at 4°C with rotation for 1 hour ...
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bioRxiv - Genetics 2024Quote: ... its antibody was removed by stripping buffer (Takara Bio #T7135A) and re-blotted by PKcs antibody to quantify the total amount of DNA-PKcs (See Figures S2I-L).
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bioRxiv - Immunology 2024Quote: ... 1 ng RNA was used as input to generate cDNA with the SMART-Seq v4 Ultra Low Input RNA Kit (Takara), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The isolated RNA was RNase III fragmented and adaptor-ligated using PrepX mRNA Library kit (Takara, Mountain View CA), then converted into cDNA using Superscript III reverse transcriptase (Lifetech ...
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bioRxiv - Genomics 2024Quote: ... were then synthesised by GenScript and initially cloned into pLVX-IRES-ZsGreen1 lentiviral vector (Takara Bio) in frame with an IRES-ZsGreen1 sequence ...
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bioRxiv - Genomics 2024Quote: We applied three distinct cDNA library enrichment protocols for the RNA sample extracted from the same mice individual: i) standard PacBio Clontech SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc.); ii ...
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bioRxiv - Immunology 2024Quote: ... The resulting plasmid containing the 3XFlag-ZBP1 was further subcloned in the Bst1 and BamHI sites of the vector pLVX-EF1α-AcGFP1-C1 (Takara, cat# 631984, simplified as pLvx-GFP) by PCR to generate the lentivirus overexpressing vector pLVX-GFP-3XFlag-ZBP1 (simplified as pLvx-GFP-ZBP1OE) ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from 1 µg of each RNA sample using PrimeScript RT Reagent Kit with gDNA Eraser (Takara), and the primers used for qRT-PCR were designed using Primer3web ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: RNA lysate was prepared at different time points post-infection using RNAiso Plus reagent (TaKaRa, Japan). Total RNA was extracted using the phenol-chloroform extraction method and 1 µg of total RNA was reverse transcribed into cDNA using a high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: ... Pre-incubated samples were loaded onto the Capturem protein A columns (TaKaRa) and centrifuged at 1000g for 1 minute at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Heparan sulfate sulfotransferase genes (in pDONR223 from the ORFeome v8.1 collection (Dharmacon)) were cloned by LR reaction (ThermoFischer Scientific) into mammalian expression plasmid pDest-eGFP-N1 (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the different fragments were amplified with the PrimeSTAR® HS polymerase (Takara Bio) using specific primers flanked by restriction sites ...
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bioRxiv - Microbiology 2024Quote: ... PCR amplification conditions were 1 µL of diluted 1/10 RT reaction with 0.05 U of PrimeSTAR GXL polymerase (Takara Bioscience), 250 nM of each primer ...
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bioRxiv - Microbiology 2024Quote: ... MTase expression was then induced at 15°C according to the Cold Shock Expression System pColdTM DNA’s protocol (Takara Bio). 24 hours after the induction ...
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bioRxiv - Microbiology 2024Quote: ... according to the supplier’s protocol (ClonTech; Protocol-at-a-Glance, PT5056-2). The E.coli strains were cultured in LB broth medium supplemented with ampicillin ...
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bioRxiv - Microbiology 2024Quote: ... The recombinant construct was transformed into Escherichia coli StellarTM Competent Cells (dam–/dcm–) (Takara Bio) according to the supplier’s protocol (ClonTech ...
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bioRxiv - Microbiology 2024Quote: ... pCMV-Myc-N (635689; Clontech, Mountain View, CA, USA), and pEGFP-C3 (#6082-1 ...
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bioRxiv - Microbiology 2024Quote: ... and pEGFP-C3 (#6082-1; Biosciences Clontech). BECN1 amplified from 3D4/21 cells was separately inserted into vector pCMV-Myc-N ...
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bioRxiv - Microbiology 2024Quote: ... High fidelity KOD DNA polymerase (Takara) was used to amplify the entire VSV G and GP64 ORFs (WT and ΔY ...
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bioRxiv - Microbiology 2024Quote: ... using PrimeScript RT (TAKARA) and random hexamers ...
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bioRxiv - Microbiology 2024Quote: ... GFP4 -and GFP7 expressing vector using In-Fusion cloning kit (Takara) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Cloning to vectors was performed with In-Fusion cloning (Takara) kit according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Illumina libraries were prepared using DNA SMARTTM ChIP-Seq Kit (TAKARA) following manufacture’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... using the following conditions: 1 µL of diluted 1/10 RT reaction with 0.05 µL of PrimeSTAR GXL polymerase (Takara Bioscience), 250 nM of each primer ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of PrimeSTAR GXL DNA polymerase (Takara), 200 uM of each dNTP ...
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bioRxiv - Microbiology 2024Quote: ... using Infusion (Takara) and transformed into Stellar cells ...
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bioRxiv - Microbiology 2024Quote: ... and HHT1_U and HHT1_L for HHT1 (Table 3) using SYBR® Premix Ex TaqTM (Perfect Real Time) (Takara Bio Inc.) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... Premix EX Taq™ Hot Start Version (TaKaRa Bio) was used for the DNA polymerase in the PCR ...
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bioRxiv - Microbiology 2024Quote: ... The guides were introduced into the pEcgRNA vector through reverse PCR using the CloneAmp HiFi PCR Premix (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the LHA and RHA PCR products were used as primers for amplifying the tsp exchange gene using CloneAmp HiFi PCR Premix (Takara Bio). After five PCR cycles ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004 ...
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bioRxiv - Immunology 2024Quote: ... Two-step PCR to amplify gag or pol region was performed with eight different primer pairs (Supplemental Table 1) and Ex-Taq (TaKaRa-Bio) as described previously (17) ...
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bioRxiv - Immunology 2024Quote: ... samples were prepared according to the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... samples were prepared according to the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... urine and kidney were carried out using NucleoSpin tissue kit (Clontech, Mountain View, CA) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2024Quote: ... 293T-GP2s (Clontech/Takara, RRID:CVCL_WI48) were transfected with 100ng MSCV plasmid (MSCV multiple cloning site IRES puroR 2A mCherry or GFP ...
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bioRxiv - Immunology 2024Quote: ... 293T-GP2s (Clontech/Takara, RRID:CVCL_WI48) were transfected with 100ng MSCV plasmid (MSCV multiple cloning site IRES puroR 2A mCherry or GFP ...
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bioRxiv - Immunology 2024Quote: ... employing SYBR Premix Ex Taq (Takara Bio), and the specific primer sequences used are detailed in Table I ...
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bioRxiv - Immunology 2024Quote: ... the isolated RNAs underwent treatment with RNase-free DNase I (Takara Bio, Otsu, Shiga, Japan) to remove any contaminating DNA ...
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bioRxiv - Immunology 2024Quote: ... The virus particles were then purified using the Aneno-X Maxi Purification Kit (Takara Bio), and the buffer was replaced with 1X Formulation Buffer containing 2.5% glycerol (w/v) ...
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bioRxiv - Immunology 2024Quote: ... The virus titer was measured using Adeno-X Rapid Titer Kit (Takara Bio) and 108 pfu (plaque-forming units ...
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bioRxiv - Immunology 2024Quote: ... the 293GP culture supernatants were filtered through a 0.45 µm syringe filter and loaded onto Retronectin (Takara Bio)-coated 24-well plates (1 ml per well ...