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Citations for Takara Bio :
951 - 1000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... transformed into the Saccharomyces cerevisiae Y187 strain (prey strain) was screened using the Matchmaker® Gold Yeast Two-Hybrid System (Takara Bio). For that purpose ...
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bioRxiv - Evolutionary Biology 2024Quote: ... according to manufacturer’s recommendations and using primers specifically designed for the In-Fusion cloning system (Takara Bio, Mountain View CA, USA). The amplified PCR fragments were recombined into the yeast bait two-hybrid vector pGBKT7 (Takara Bio ...
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bioRxiv - Evolutionary Biology 2024Quote: ... adhaerens Mint PDZ-1/PDZ-2/P1BM protein sequences (as described in the results section) were cloned into the mammalian bicistronic expression vector pIRES2-DsRed2 (Clontech, USA) to express recombinant proteins bearing C-terminal myc epitope tags (Figure 6K) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and subsequently restriction digest cloned into the vector pEGFP-C1 (Clontech) to express recombinant fusion proteins bearing an N-terminal Enhanced Green Fluorescent Protein (EGFP ...
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bioRxiv - Genomics 2024Quote: ... Membranes were then incubated overnight on a shaker at 4°C in primary antibodies (ß-actin CST3700S; MLH1 Invitrogen MA5-32041; Cas9 Takara 632607) diluted 1:1000 in 1x TBST with 3% BSA ...
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bioRxiv - Immunology 2024Quote: 293GP cells (Takara Bio, 631458), Nalm6 (ATCC ...
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bioRxiv - Immunology 2024Quote: ... employing SYBR Premix Ex Taq (Takara Bio), and the specific primer sequences used are detailed in Table I ...
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bioRxiv - Immunology 2024Quote: ... the isolated RNAs underwent treatment with RNase-free DNase I (Takara Bio, Otsu, Shiga, Japan) to remove any contaminating DNA ...
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bioRxiv - Genomics 2024Quote: ... Stellar competent cells (Takara) were used for transformation and downstream miniprep (Qiagen) ...
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bioRxiv - Genomics 2024Quote: ... Library construction was performed based on the manufacturer’s recommendation for SmarterStranded V2 kit (Takara Bio, California, USA). The final library quantity was measured by KAPA SYBR FAST qPCR and library quality was evaluated by TapeStation D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Genetics 2024Quote: ... wherein 2.5 µg of pooled plasmid was diluted with Xfect reaction buffer (Takara Bio, 631318) to a total volume of 50 μL ...
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bioRxiv - Immunology 2024Quote: ... cDNA libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... into which a DNA fragment was inserted by In-Fusion HD Cloning Kit (TAKARA) to produce RCAS-GCaMP6s-P2A-mRuby3 ...
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bioRxiv - Developmental Biology 2024Quote: ... The SmartSeq v4 kit (Clontech) was used for reverse transcription and cDNA amplification ...
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bioRxiv - Developmental Biology 2024Quote: ... and/or 1:200 anti-N-cadherin antibodies (TAKARA, M110) in 1% blocking regent (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... In-nuclei chromatin digestion to achieve 80 % monomer / 20 % oligomers nucleosome ratio was performed with MNase (Takara Bio, 2910a) 100 U per 4M nuclei for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Foamy virus was produced using Lenti-X 293T (Takara Bio, Kusatsu) generated as previously described and frozen at -80°C for long term storage42 ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were heat-shock transformed into stellar competent cells (Takara Bio, Kusatsu). SOC outgrowth was performed for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were generated and sequencing was performed by the NY Genome Technology Center with a low input SMART-Seq HT with Nxt HT kit (Clontech Laboratories, 634947) and SP100 cycle flow cell ...
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bioRxiv - Developmental Biology 2024Quote: ... genomic DNA removal and reverse transcription were carried out using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara Bio).
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bioRxiv - Developmental Biology 2024Quote: ... was done using a SYBR Premix Ex TaqII Dimer Eraser and a Thermal Cycler Dice Real Time System III (Takara Bio). The gene encoding elongation factor 1α (EF1α ...
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bioRxiv - Developmental Biology 2024Quote: The open reading frame (ORF) of Kaede was removed from pSPCiPC2Kaede by inverse PCR with PrimeStar GxL DNA polymerase (Takara-bio Inc). The ORFs of Gαq ...
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bioRxiv - Developmental Biology 2024Quote: ... These PCR fragments were fused with the In-Fusion HD Cloning kit (Clontech). The region encoding XY linker43 was deleted from PLCβ1/2/3 cDNA by inverse PCR to create a constitutively active form ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Reporter plasmid libraries were made by cloning amplified ATAC fragments into AgeI-HF- and SalI-HF-linearized pSTARR-seq plasmid using InFusion HD cloning kit (Takara) and then propagated in MegaX DH10B T1R electrocompetent bacteria ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into the vector PCS2+ to generate the expression constructs (5x In-Fusion HD Enzyme Premix, Takara
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bioRxiv - Developmental Biology 2024Quote: ... For beta-catenin overexpression the CHIR99021 was substituted with 1 µg/ml doxycycline (Clontech).
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bioRxiv - Developmental Biology 2024Quote: ... cDNA libraries were then synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech; PT4097-1) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... low-passage 3.5×106 Hek293T Lenti-X cells (632180, Takara) were seeded per 10 cm dish (Corning ...
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bioRxiv - Developmental Biology 2024Quote: ... Hek293T Lenti-X cells (632180, Takara) were used for making lentivirus ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed with the SYBR Premix Ex Taq kit (TaKaRa) with Rplp0 serving as the internal control ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used were CDH1 (E-CADHERIN) (M106, Takara Biosciences) for glandular and luminal epithelial staining ...
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bioRxiv - Developmental Biology 2024Quote: Using the Cogent NGS Analysis Pipeline software v 1.5.1 (Takara), FASTQ files containing all indices for each chip were demultiplexed to FASTQ files containing one index per file ...
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bioRxiv - Developmental Biology 2024Quote: ... for 20 minutes on ice and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara ...
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bioRxiv - Developmental Biology 2024Quote: ... The library for sequencing was then prepared using the SMART-Seq ICELL8 application kit (Takara) following the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... the SMART-seq v4 Ultra Low Input RNA kit (Takara) was used to prepare cDNAs starting from 1.5 µg of total RNA for each sample and sequencing libraries were prepared subsequently prepared using the Ovation Ultralow System V2 (NuGen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara) with the green and not red logic ...
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bioRxiv - Developmental Biology 2024Quote: ... linker italics) were used to PCR amplify (Takara PrimeStar Max) the homology repair template (HRT) ...
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bioRxiv - Developmental Biology 2024Quote: ... Library construction started from 4.9ng of RNA per sample made using the SMARTer Stranded Total RNA-seq Kit v3-Pico Input Mammalian (Takara; 634486). The quality of libraries was checked with the with the High Sensitivity DNA Reagents Kit (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-Ds-Red (rabbit, 632496; Clontech; 1:500), anti-mCherry (chicken ...
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bioRxiv - Genetics 2024Quote: ... The lentivirus was concentrated using the Lenti-X concentrator (Takara Bio). The viral titer was determined by performing lentiviral transductions at varying concentrations in a 48-well plate format ...
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bioRxiv - Genetics 2024Quote: Lentivirus was produced in the Lenti-X 23T cell line (Takara Bio). Transfection was carried out with psPAX2 (Addgene #12260) ...
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bioRxiv - Immunology 2024Quote: ... libraries were prepared using Clontech SMARTer stranded v2 Pico Mammalian kit (Takara, San Jose, CA) with total RNA (50-100ng ...
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bioRxiv - Immunology 2024Quote: ... The DNA fragment isolated from pCR3.1-mC/EBPα by NheI/XhoI-digestion was inserted into NheI/XhoI-digested pIRES2-AcGFP1 (Clontech, Takara) to generate pIRES2-AcGFP1-mC/EBPα.
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bioRxiv - Immunology 2024Quote: ... The DNA fragment isolated from pCR3.1-mC/EBPα by NheI/XhoI-digestion was inserted into NheI/XhoI-digested pIRES2-AcGFP1 (Clontech, Takara) to generate pIRES2-AcGFP1-mC/EBPα.
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bioRxiv - Immunology 2024Quote: ... The amplified DNA fragments were ligated into T vector pMD19 (#3271, Takara), and whose nucleotide sequences in each plasmid clones were determined.
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bioRxiv - Genetics 2024Quote: ... PCR was conducted as described earlier (section: RNAi-mediated knockdown of Pmtra-2) but using the proofreading PrimeSTAR HS DNA Polymerase (Takara Bio, Shiga, Japan) and Ex Taq DNA Polymerase (Takara Bio ...
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bioRxiv - Genetics 2024Quote: ... and Ex Taq DNA Polymerase (Takara Bio, Shiga, Japan) respectively in the 1st- and 2nd-round PCR ...
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bioRxiv - Immunology 2024Quote: ... banked cell pellets were rapidly thawed and processed using the Nucleospin® Blood XL kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and Ex Taq polymerase (Takara) per the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.