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Citations for Takara Bio :
951 - 1000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... linearized GPC sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: The WOo DNA methyltransferase was artificially synthesised with codon usage adaptation and cloned into the XhoI and SalI sites of the pCOLD III expression vector (Takara Bio). The recombinant construct was transformed into Escherichia coli StellarTM Competent Cells (dam–/dcm– ...
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bioRxiv - Genetics 2024Quote: ... The lentivirus was concentrated using the Lenti-X concentrator (Takara Bio). The viral titer was determined by performing lentiviral transductions at varying concentrations in a 48-well plate format ...
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bioRxiv - Genetics 2024Quote: Lentivirus was produced in the Lenti-X 23T cell line (Takara Bio). Transfection was carried out with psPAX2 (Addgene #12260) ...
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bioRxiv - Genomics 2024Quote: Approximately 4×106 HAP1 cells were transfected using Xfect Transfection Reagent (#631318, Takara Bio) for each pSpCas9(BB)-2A-Puro construct ...
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bioRxiv - Genomics 2024Quote: ... The polymerase chain reaction (PCR) was performed on genomic DNA to amplify 16s rRNA using Emerald Amp MAX PCR Master Mix (Takara Bio) using the primers presented in Table S1.
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bioRxiv - Genomics 2024Quote: ... and one microgram of the DNase-treated RNA was used for cDNA synthesis using PrimeScript RT reagent kit (Takara Bio, CA, USA). The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio ...
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bioRxiv - Genomics 2024Quote: ... The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio, CA, USA) on Bio-Rad CFX 96 C1000 with following conditions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the PCR master mix was prepared using Z-Taq (Takara R006B) and primers (Table S1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we then added 1 mL of Super Optimal Broth with Catabolite Repression Medium (SOC, provided with the electrocompetent cells (Takara, Japan, product #9027), shaking the cells at 230 RPM at 37°C for 1.5 hours (Infors HT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli DH5α electrocompetent cells (Takara, Japan, product #9027) by adding 2 uL of (unpurified ...
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bioRxiv - Genomics 2024Quote: ... with three markers (λ-Hind III digest; Takara Cat ...
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bioRxiv - Genomics 2024Quote: ... with three markers (λ-Hind III digest; Takara Cat. No. 3403, DL15,000 DNA Marker; Takara Cat ...
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bioRxiv - Genetics 2024Quote: Two μg of total RNA was reverse transcribed into cDNA templates using RNA to cDNA EcoDry™ Premix kit including both random hexamer and oligo(dT)18 primers (Takara Bio, 639548). KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Genetics 2024Quote: ... and NucleoSpin® RNA set for NucleoZOL™ (Takara Bio, 740406.50) following the manufactures specifications ...
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bioRxiv - Genetics 2024Quote: ... and NucleoSpin® RNA Clean-up XS (Takara Bio, 740903) for RNA repurification ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The amplified PCR fragments were recombined into the yeast bait two-hybrid vector pGBKT7 (Takara Bio, Kusatsu, Japan), previously digested with EcoRI and BamHI ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and pGADT7-murine p53 (Takara Bio) were used as negative controls ...
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bioRxiv - Evolutionary Biology 2024Quote: ... thaliana Col-0 cDNA library (Takara Bio) transformed into the Saccharomyces cerevisiae Y187 strain (prey strain ...
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bioRxiv - Evolutionary Biology 2024Quote: ... transformed into the Saccharomyces cerevisiae Y187 strain (prey strain) was screened using the Matchmaker® Gold Yeast Two-Hybrid System (Takara Bio). For that purpose ...
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bioRxiv - Evolutionary Biology 2024Quote: ... according to manufacturer’s recommendations and using primers specifically designed for the In-Fusion cloning system (Takara Bio, Mountain View CA, USA). The amplified PCR fragments were recombined into the yeast bait two-hybrid vector pGBKT7 (Takara Bio ...
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bioRxiv - Evolutionary Biology 2024Quote: ... adhaerens Mint PDZ-1/PDZ-2/P1BM protein sequences (as described in the results section) were cloned into the mammalian bicistronic expression vector pIRES2-DsRed2 (Clontech, USA) to express recombinant proteins bearing C-terminal myc epitope tags (Figure 6K) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and subsequently restriction digest cloned into the vector pEGFP-C1 (Clontech) to express recombinant fusion proteins bearing an N-terminal Enhanced Green Fluorescent Protein (EGFP ...
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bioRxiv - Genomics 2024Quote: ... Membranes were then incubated overnight on a shaker at 4°C in primary antibodies (ß-actin CST3700S; MLH1 Invitrogen MA5-32041; Cas9 Takara 632607) diluted 1:1000 in 1x TBST with 3% BSA ...
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bioRxiv - Genomics 2024Quote: ... Final libraries were purified with Nucleospin PCR Clean up kit (Takara) and quantified by Qubit HS (Thermo Scientific) ...
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bioRxiv - Genomics 2024Quote: ... we repurposed the SMARTer smRNA-seq kit (Takara). First ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All yeast 2-hybrid screens were done using the Matchmaker® Gold Yeast Two-Hybrid System (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... media was aspirated and concentrated using Lenti-X Concentrator (TaKaRa #631231) following the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2024Quote: Lenti-X 293T was purchased from Takara (632180) and K562 (CCL-243 ...
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bioRxiv - Genetics 2024Quote: ... all media were supplemented with 1µM Shield1 ligand (Takara, #632189) to stabilize both reporters ...
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bioRxiv - Genetics 2024Quote: ... using NucleoZOL™ (Takara Bio, 740404.200) and NucleoSpin® RNA set for NucleoZOL™ (Takara Bio ...
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bioRxiv - Genetics 2024Quote: ... followed by rDNase Set (Takara Bio, 740963) to digest DNA ...
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bioRxiv - Immunology 2024Quote: ... The DNA fragment isolated from pCR3.1-mC/EBPα by NheI/XhoI-digestion was inserted into NheI/XhoI-digested pIRES2-AcGFP1 (Clontech, Takara) to generate pIRES2-AcGFP1-mC/EBPα.
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bioRxiv - Immunology 2024Quote: ... The DNA fragment isolated from pCR3.1-mC/EBPα by NheI/XhoI-digestion was inserted into NheI/XhoI-digested pIRES2-AcGFP1 (Clontech, Takara) to generate pIRES2-AcGFP1-mC/EBPα.
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bioRxiv - Immunology 2024Quote: ... The amplified DNA fragments were ligated into T vector pMD19 (#3271, Takara), and whose nucleotide sequences in each plasmid clones were determined.
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bioRxiv - Immunology 2024Quote: 293GP cells (Takara Bio, 631458), Nalm6 (ATCC ...
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bioRxiv - Immunology 2024Quote: ... Lentivirus was produced by transfecting 95 % confluent Lenti-X cells (Takara) grown in wells of 6-well plates with 1000 ng psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Immunology 2024Quote: ... A stable IFN-λ-expressing cell line was obtained by transfection of one million Lenti-X (HEK293T, Takara) cells with 500 ng Super PiggyBac Transposase vector and 1250 ng of PiggyBac transposase mammalian expression vector using Lipofectamine 3000 following to the manufactureŕs instructions ...
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bioRxiv - Genomics 2024Quote: ... Supernatant containing viral particles was harvested after 2 and 3 days and concentrated 100x using Lenti-X concentrator (Takara) following manufacturers instructions.
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bioRxiv - Genomics 2024Quote: Lentivirus was produced in LentiX HEK293T cells (Takara). Cells were grown to 65–75% confluency prior to transfection ...
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bioRxiv - Immunology 2024Quote: Total cellular or skin RNA was extracted using Trizol reagent (9108, Takara), and cDNA was synthesized by using the Reverse Transcriptase kit (RR037A ...
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bioRxiv - Immunology 2024Quote: ... Real-Time PCR was performed by using specific primers and SYBR Green ROX Mix (RR420A, Takara). β-actin was used as the internal quantitative control.
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bioRxiv - Immunology 2024Quote: ... and cDNA was synthesized by using the Reverse Transcriptase kit (RR037A, Takara). Real-Time PCR was performed by using specific primers and SYBR Green ROX Mix (RR420A ...
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bioRxiv - Immunology 2024Quote: ... lentivirus was precipitated from the supernatant using Lenti-X Concentrator (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... libraries were prepared using Clontech SMARTer stranded v2 Pico Mammalian kit (Takara, San Jose, CA) with total RNA (50-100ng ...
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bioRxiv - Genomics 2024Quote: ... and concentrated to 50x in 1x PBS using Lenti-X Concentrator (Clontech, 631232) in accordance with the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... then concentrated to 50x in 1x PBS using Lenti-X Concentrator (Takara, 631232), in accordance with the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Virus was concentrated using Lenti-X Concentrator (Takara) and titer quantified using p24 ELISA antigen assay (Takara) ...
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bioRxiv - Genomics 2024Quote: ... Stellar competent cells (Takara) were used for transformation and downstream miniprep (Qiagen) ...
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bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.