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Citations for Takara Bio :
851 - 900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The mouse PG cDNA was generated by PrimeSTAR Max DNA Polymerase (Takara) to skip 150 bp ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated with a 1:2000 dilution of an anti-GFP antibody (Clontech 632381, clone JL8) for detection of Clover protein ...
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bioRxiv - Cell Biology 2024Quote: ... The mCherry sequence from pmCherry-C1 (Clontech) was amplified to introduce flanking NheI sites using the following primers:
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... Cloning was performed using In-Fusion HD Cloning Mix (Takara). Restriction enzymes were purchased from New England Biolabs (NEB) ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... All mutagenesis was done using instructions from In-Fusion HD Cloning Mix (Takara) unless noted below.
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bioRxiv - Cell Biology 2024Quote: ... pVSV-G (PT3343-5, Clontech) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Cell Biology 2024Quote: ... pLmna-Em-PG-R26Neo and pLmna-Em-LC-R26Neo were linearized by the Kpn I (Takara) and purified by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Biochemistry 2024Quote: ... was obtained from Clontech. The hSSB1 coding sequence was amplified by PCR and cloned upstream of EGFP to fuse hSSB1 to the N-terminus of EGFP ...
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bioRxiv - Biochemistry 2024Quote: ... PCR amplifications for synthetic genes and a linear-pET-22b (+) vector were performed with using PrimeSTAR® Max DNA polymerase (TaKaRa) and the designed primers (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... total mouse liver mRNA (Takara) was used.
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 anti-tdtomato rabbit primary antibody (#632496 from Takara), 1:1000 anti β-tubulin mouse primary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... (1:1000 anti GAPDH) (1:1000 anti-TdTomato rabbit primary antibody #632496 from Takara).
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bioRxiv - Biochemistry 2024Quote: Site-directed mutagenesis was achieved by Inverse PCR using PrimeSTAR® Max DNA polymerase (TaKaRa) with the designed primers (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal quantities (5 ng) of total RNA from each sample were used for cDNA synthesis using the PrimeScript®RT reagent kit (TaKaRa, Tokyo, Japan). The reverse transcriptions of miRNAs were performed using looped miRNA-specific RT primers for miRNAs ...
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bioRxiv - Genetics 2024Quote: ... Approximately 0.3 mg of total RNA was converted into cDNA with the PrimeScript RT Reagent Kit (Takara, Japan, RR037A) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from Total RNA using the SMART-Seq v4 Ultra low Input RNA kit (Takara Bio) and quantified using Bioanalyzer (Agilent ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... A Flag tag x3 was inserted between amino acids 489 and 490 and a HA tag was inserted between amino acids 724 and 725 using In-Fusion Cloning Mix (Takara).
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bioRxiv - Cell Biology 2024Quote: ... device using TB Green Premix Ex Taq II (Tli RNase H Plus, Takara) and the gene expression was normalized to Cyclophilin and presented ...
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bioRxiv - Pathology 2024Quote: ... Real-time PCR was performed by using an SYBR Green PCR Master Mix (TaKaRa, Dalian, China) and the primers listed in Appendix B.
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bioRxiv - Pathology 2024Quote: First strand cDNA was generated from 1 μg of total RNA using the Prime Script RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). Real-time PCR was performed by using an SYBR Green PCR Master Mix (TaKaRa ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-Dsred (Takara, 632496), rabbit anti-VAChT (SYSY ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was produced by Lenti-X™ 293T cells (Takara Bio USA), transfected with lentiviral expression vector (330 ng) ...
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bioRxiv - Cell Biology 2024Quote: ... the titers of the generated AAV vectors were determined by Takara-AAVpro Titration Kit (for Real Time PCR) ...
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bioRxiv - Cell Biology 2024Quote: ... Ver.2 (Takara, cat# 6233), as per manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... Viruses were purified with AAVpro Purification Kit Maxi (All Serotypes) (Takara, cat# 6666), as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and 1μg/ml doxycycline (Clontech). Cells were incubated at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng of isolated gDNA was amplified using the PrimeSTAR MAX PCR mix (Takara) using the primers gRNA_screening_FW and gRNA_screening_RV (Table1) ...
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bioRxiv - Cell Biology 2024Quote: ... using Xfect (Takara) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... transfected at 6–8 hours with 0.75 μg of DNA using Xfect Transfection reagent (Clontech) and then analysed 2 days after.
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bioRxiv - Cell Biology 2024Quote: iPSCs SFCi55 and hESCs RUNX1-GFP were plated at 3 × 105 cells per a well of a 6 well plate and reverse transfected with 2 μg of DNA using the Xfect Transfection reagent (Clontech) and analyzed 2 days later.
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative PCR (qPCR) was performed on the CFX Connect Real-Time PCR Detection System using SYBR Green PCR Master Mix (TaKaRa). Primer pairs used were listed in Supplementary (Table S2) ...
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bioRxiv - Developmental Biology 2024Quote: ... PrimeStar Max (Takara) or PrimeStar GXL (Takara ...
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bioRxiv - Developmental Biology 2024Quote: ... Lenti-X 293T cells were acquired from Clontech (632180). Adult dermal fibroblasts were acquired from Coriell (GM02171 ...
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bioRxiv - Developmental Biology 2024Quote: ... or PrimeStar GXL (Takara) were used ...
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bioRxiv - Developmental Biology 2024Quote: ... were used to PCR amplify the homology repair template (Takara PrimeStar Max). After purification (Promega Wizard SV Gel and PCR clean-up system) ...
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bioRxiv - Cell Biology 2024Quote: ... A bicistronic construct expressing human EPAC1b with a C-terminal His10 tag and SUMO3(Q89K) was constructed using the pIRES2-EGFP vector (Clontech Catalog no. 632435). The EPAC1-His10-IRES-SUMO3(Q89K ...
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bioRxiv - Cell Biology 2024Quote: ... were sub-cloned from pCMV-HA-BTN3A2-L and pCMV-HA-BTN3A2-S expression vectors into the pLVX-tight-puro vector (632162, Clontech, USA) (Supplementary Table S1) ...
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bioRxiv - Cell Biology 2024Quote: ... The TETon system was combined with a separate lentiviral compatible vector containing the reverse tetracycline-controlled transactivator Advance (rtTA Advance) (632162, Clontech, USA). Lentiviral particles corresponding to either TETon-BTN3A2 or rtTA Advance were generated using the above procedures ...
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bioRxiv - Cell Biology 2024Quote: A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. The PCR primers introduced BamHI and NotI sites at the 5’ and 3’ ends respectively ...
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bioRxiv - Cell Biology 2024Quote: ... where the entire ORF of the corresponding gene was inserted in frame into the pGADT7 (GAL4 activation domain) or pGBKT7 (GAL4 binding domain) plasmid (Clontech).
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bioRxiv - Cell Biology 2024Quote: ... Two-hybrid interaction was tested with YNB medium lacking histidine in Saccharomyces cerevisiae strain AH109 (Clontech).
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were loaded on 5 mL of TALON beads (Takara Bio) pre-equilibrated with the lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... in a final volume of 600 μl for 15 min before the transfection mix was added to subconfluent (80-90%) Lenti-X 293T cells (Clontech) grown on a 10-cm tissue culture dish ...
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bioRxiv - Cell Biology 2024Quote: ... The virus titer was determined using Lenti-X GoStix Plus (Clontech). To transduce hTCEpi cells ...
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bioRxiv - Cell Biology 2024Quote: ... clarified supernatant was combined with Lenti-X concentrator (Clontech) at a 3-to-1 volume ratio ...
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bioRxiv - Cell Biology 2024Quote: ... Three nanograms of total RNA were used for amplification using the SMART-Seq V4 Ultra Low Input RNA kit (Clontech) according to the manufacturer’s recommendations (10 PCR cycles were performed) ...
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bioRxiv - Developmental Biology 2024Quote: ... genomic DNA removal and reverse transcription were carried out using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara Bio).
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bioRxiv - Developmental Biology 2024Quote: ... was done using a SYBR Premix Ex TaqII Dimer Eraser and a Thermal Cycler Dice Real Time System III (Takara Bio). The gene encoding elongation factor 1α (EF1α ...
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bioRxiv - Developmental Biology 2024Quote: The open reading frame (ORF) of Kaede was removed from pSPCiPC2Kaede by inverse PCR with PrimeStar GxL DNA polymerase (Takara-bio Inc). The ORFs of Gαq ...
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bioRxiv - Developmental Biology 2024Quote: ... These PCR fragments were fused with the In-Fusion HD Cloning kit (Clontech). The region encoding XY linker43 was deleted from PLCβ1/2/3 cDNA by inverse PCR to create a constitutively active form ...