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Citations for Takara Bio :
901 - 950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... where the entire ORF of the corresponding gene was inserted in frame into the pGADT7 (GAL4 activation domain) or pGBKT7 (GAL4 binding domain) plasmid (Clontech).
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bioRxiv - Cell Biology 2024Quote: ... Two-hybrid interaction was tested with YNB medium lacking histidine in Saccharomyces cerevisiae strain AH109 (Clontech).
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were loaded on 5 mL of TALON beads (Takara Bio) pre-equilibrated with the lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... in a final volume of 600 μl for 15 min before the transfection mix was added to subconfluent (80-90%) Lenti-X 293T cells (Clontech) grown on a 10-cm tissue culture dish ...
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bioRxiv - Cell Biology 2024Quote: ... The virus titer was determined using Lenti-X GoStix Plus (Clontech). To transduce hTCEpi cells ...
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bioRxiv - Cell Biology 2024Quote: ... clarified supernatant was combined with Lenti-X concentrator (Clontech) at a 3-to-1 volume ratio ...
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bioRxiv - Cell Biology 2024Quote: ... Three nanograms of total RNA were used for amplification using the SMART-Seq V4 Ultra Low Input RNA kit (Clontech) according to the manufacturer’s recommendations (10 PCR cycles were performed) ...
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bioRxiv - Cell Biology 2024Quote: ... was amplified and analysed in real-time polymerase chain reaction on a Roche Light Cycler 480 system with TB Green® Premix Ex Taq™ II (TaKaRa Bio Inc., Japan). The specific primers were predesigned and validated by PrimerBank of Massachusetts General Hospital (Spandidos et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Domain swapped constructs were generated by in-fusion cloning (Takara Bio). FZD(1-10)-V5-IRES-mKate constructs were a kind gift of Karl Willert (Department of Cellular & Molecular Medicine ...
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bioRxiv - Cell Biology 2024Quote: ... SyBrGreen (Takara) was used to perform RT‒PCR ...
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bioRxiv - Cell Biology 2024Quote: ... and cDNA was synthesized with Superscript III cDNA-synthesis Kit (Takara). SyBrGreen (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-mCherry (632543, Clontech; 1:500), mouse anti-GFP (ab1218 ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis and amplification were performed using a SMART-Seq v4 Ultra Low Input RNA Kit for sequencing (Clontech). cDNA was purified using an Agencourt AMPure purification kit (Beckman Coulter) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted with Triol Reagent (Takara), and cDNA was synthesized with Superscript III cDNA-synthesis Kit (Takara) ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: RNA lysate was prepared at different time points post-infection using RNAiso Plus reagent (TaKaRa, Japan). Total RNA was extracted using the phenol-chloroform extraction method and 1 µg of total RNA was reverse transcribed into cDNA using a high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: ... Pre-incubated samples were loaded onto the Capturem protein A columns (TaKaRa) and centrifuged at 1000g for 1 minute at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... MTase expression was then induced at 15°C according to the Cold Shock Expression System pColdTM DNA’s protocol (Takara Bio). 24 hours after the induction ...
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bioRxiv - Microbiology 2024Quote: ... according to the supplier’s protocol (ClonTech; Protocol-at-a-Glance, PT5056-2). The E.coli strains were cultured in LB broth medium supplemented with ampicillin ...
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bioRxiv - Microbiology 2024Quote: ... The recombinant construct was transformed into Escherichia coli StellarTM Competent Cells (dam–/dcm–) (Takara Bio) according to the supplier’s protocol (ClonTech ...
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bioRxiv - Microbiology 2024Quote: ... Quantified PCR was performed using SYBR Premix Ex Taq Kit (Takara Bio Inc., Japan), and carried out on ABI Prism 7000 sequence detection system (Applied Biosystems USA) ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the RNAiso Plus kit (Takara, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: RNA extract used for sequencing above was treated with RNase-Free DNase (Takara) to remove contaminated DNA ...
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bioRxiv - Microbiology 2024Quote: ... and cDNA was synthesized using the M-MLV Reverse Transcriptase cDNA Synthesis Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... membrane and concentrated up to 10-fold using Lenti-X Concentrator (TaKaRa). The virus pellet was resuspended in 100 µL of Phosphate Buffered Saline (PBS ...
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bioRxiv - Genetics 2024Quote: ... 0.2*106 mESCs were seeded in a well of a 12-well plate in 2iL and transduced the next day with 1ml of 5x concentrated (lenti-X, Clontech) and filtered viral supernatant with 8 ng/μl polybrene (Sigma Aldrich) ...
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bioRxiv - Genetics 2024Quote: ... oligonucleotide primers were designed to introduce the respective mutant sites using the PCR-based In-Fusion cloning technique (Takara Bio, Japan). The transgenes with human reference wildtype COL4A5 and select patient-derived variants were introduced to the 51C attP landing site on the second chromosome by Rainbow Transgenic Flies (CA ...
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bioRxiv - Genetics 2024Quote: Two μg of total RNA was reverse transcribed into cDNA templates using RNA to cDNA EcoDry™ Premix kit including both random hexamer and oligo(dT)18 primers (Takara Bio, 639548). KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Genetics 2024Quote: ... and NucleoSpin® RNA set for NucleoZOL™ (Takara Bio, 740406.50) following the manufactures specifications ...
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bioRxiv - Genetics 2024Quote: ... and NucleoSpin® RNA Clean-up XS (Takara Bio, 740903) for RNA repurification ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The amplified PCR fragments were recombined into the yeast bait two-hybrid vector pGBKT7 (Takara Bio, Kusatsu, Japan), previously digested with EcoRI and BamHI ...
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bioRxiv - Genomics 2024Quote: ... Final libraries were purified with Nucleospin PCR Clean up kit (Takara) and quantified by Qubit HS (Thermo Scientific) ...
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bioRxiv - Genomics 2024Quote: ... we repurposed the SMARTer smRNA-seq kit (Takara). First ...
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bioRxiv - Genomics 2024Quote: Approximately 4×106 HAP1 cells were transfected using Xfect Transfection Reagent (#631318, Takara Bio) for each pSpCas9(BB)-2A-Puro construct ...
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bioRxiv - Genomics 2024Quote: ... The polymerase chain reaction (PCR) was performed on genomic DNA to amplify 16s rRNA using Emerald Amp MAX PCR Master Mix (Takara Bio) using the primers presented in Table S1.
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bioRxiv - Genomics 2024Quote: ... and one microgram of the DNase-treated RNA was used for cDNA synthesis using PrimeScript RT reagent kit (Takara Bio, CA, USA). The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio ...
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bioRxiv - Genomics 2024Quote: ... The expression analysis was performed using TB green Premix Ex Taq II (Takara Bio, CA, USA) on Bio-Rad CFX 96 C1000 with following conditions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the amplified DNA samples were processed with S1 nuclease (TaKaRa) to reduce branching junctions.
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bioRxiv - Evolutionary Biology 2024Quote: ... the PCR master mix was prepared using Z-Taq (Takara R006B) and primers (Table S1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we then added 1 mL of Super Optimal Broth with Catabolite Repression Medium (SOC, provided with the electrocompetent cells (Takara, Japan, product #9027), shaking the cells at 230 RPM at 37°C for 1.5 hours (Infors HT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli DH5α electrocompetent cells (Takara, Japan, product #9027) by adding 2 uL of (unpurified ...
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bioRxiv - Genomics 2024Quote: ... with three markers (λ-Hind III digest; Takara Cat ...
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bioRxiv - Genomics 2024Quote: ... with three markers (λ-Hind III digest; Takara Cat. No. 3403, DL15,000 DNA Marker; Takara Cat ...
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bioRxiv - Genetics 2024Quote: ... all media were supplemented with 1µM Shield1 ligand (Takara, #632189) to stabilize both reporters ...
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bioRxiv - Genetics 2024Quote: ... using NucleoZOL™ (Takara Bio, 740404.200) and NucleoSpin® RNA set for NucleoZOL™ (Takara Bio ...
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bioRxiv - Genetics 2024Quote: ... followed by rDNase Set (Takara Bio, 740963) to digest DNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All yeast 2-hybrid screens were done using the Matchmaker® Gold Yeast Two-Hybrid System (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... media was aspirated and concentrated using Lenti-X Concentrator (TaKaRa #631231) following the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2024Quote: Lenti-X 293T was purchased from Takara (632180) and K562 (CCL-243 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and pGADT7-murine p53 (Takara Bio) were used as negative controls ...
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bioRxiv - Evolutionary Biology 2024Quote: ... thaliana Col-0 cDNA library (Takara Bio) transformed into the Saccharomyces cerevisiae Y187 strain (prey strain ...