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Citations for Takara Bio :
801 - 850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... or 5 ng of total RNA (with a SMARTer Stranded Total RNA-Seq Kit v3; Takara Bio) was used.
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bioRxiv - Microbiology 2024Quote: ... and 20 or 25 uL were assayed using either the Great EscAPe SeAP kit (Takara/Clontech) or the Phospha-Light System (Applied Biosystems).
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bioRxiv - Immunology 2024Quote: ... All ChIP-seq and input DNA libraries were made using ThruPLEX DNA-Seq kit (cat #R400674, Takara Bio, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cDNA was prepared using the Ultra Low kit from Clontech (Mountain View, CA). Libraries were prepared at the according to manufacturer’s instructions for the NEBNext Ultra II Direction RNA kit (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... except that raw read processing by Cutadapt had to be adapted to different library construction method (SMARTer smRNA-Seq Kit, TAKARA). In particular ...
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bioRxiv - Biochemistry 2024Quote: ... The GoldenGate cloning cassette was amplified using primers listed in Table 6 using p641-BsaI as template and the PCR product was ligated with the linearized pET28a(+) backbone using InFusion (Takara) cloning.
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bioRxiv - Microbiology 2024Quote: The q-PCR standards were synthesized by cloning target genes into Escherichia coli DH5a using a PMD18-T vector Cloning Kit (TaKaRa Biotechnology, China). Vector concentrations were measured using NanoDrop ...
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bioRxiv - Microbiology 2024Quote: Total RNA was isolated from infected macrophages using RNAiso-Plus (Takara), and cDNA was prepared using the Prime Script RT reagent kit (Takara ...
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bioRxiv - Microbiology 2024Quote: ... using the Great EscAPe SeAP kit (Takara).
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bioRxiv - Microbiology 2024Quote: ... and 20 or 25 uL were assayed using either the Great EscAPe SeAP kit (Takara/Clontech) or the Phospha-Light System (Applied Biosystems).
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bioRxiv - Microbiology 2024Quote: ... using the Great EscAPe SeAP kit (Takara). T test was used to determine that only samples transfected with Rta induced significant viral reactivation (p<0.05 compared to Vec) ...
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bioRxiv - Physiology 2024Quote: The pGG2-TNNT2-HA-TTL and the pAAV-TNNT2-Empty plasmids were generated from the pGG2-TNNT2-Mut1 plasmid (VC206) by InFusion Cloning (Takara Clontech). In brief ...
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bioRxiv - Physiology 2024Quote: The pGG2-TNNT2-HA-TTL and the pAAV-TNNT2-Empty plasmids were generated from the pGG2-TNNT2-Mut1 plasmid (VC206) by InFusion Cloning (Takara Clontech). In brief ...
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bioRxiv - Physiology 2024Quote: ... Virus containing medium was collected at 48h and concentrated using Lenti-XTM Concentrator (Takara). C2C12 mouse myoblasts were seeded 24h prior to infection and then transduced with virus-containing supernatant supplemented with 8μg/mL polybrene (Millipore ...
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bioRxiv - Genetics 2024Quote: ... PCR amplifications were done in duplicates using the PrimerSTAR GXL polymerase (Takara Bio) and 250 ng of genomic DNA per reaction ...
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bioRxiv - Genetics 2024Quote: ... Real-time PCR was carried out using SYBR Premix Ex TaqTM II (Takara) on the Applied Biosystems StepOne Real-Time PCR System ...
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bioRxiv - Genetics 2024Quote: ... then they were sequenced directly or cloned into the pMD18-T Vector (TaKaRa) for sequencing.
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bioRxiv - Genetics 2024Quote: ... PCR was performed as standard procedures in 50 μl reactions by TaKaRa LA Taq ...
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bioRxiv - Developmental Biology 2024Quote: ... Two targeting plasmids were constructed with InFusion cloning (Takara Bio), one consisting of 5’ and 3’ homology arms (each 1kb in length) ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were stained with anti-mouse E-cadherin monoclonal antibody ECCD-2 (1:100, Takara) for 16 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: RNA extraction was carried out using RNAiso Plus (TaKaRa, Dalian, China) following the manufacturer’s instructions with some modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... the larvae were embedded in agarose gel (5805A, Takara) in E3 buffer supplemented with anesthesia and N-phenylthiourea and observed using a TE2000 microscope (Nikon ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we made a master mix using the primers 2550F = 5’-GTT ACT GAT TCG TCT ACG AGA-3’ and 2718R = 5’-ATT GAA ATG ATC CAG TGC TTG-3’ and TaKaRa ExTaq DNA Polymerase (Takara Bio USA, Inc). We ran the samples for 31 cycles and ran the PCR product on a 2% agarose gel.
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bioRxiv - Evolutionary Biology 2024Quote: ... rapid amplification of cDNA ends (RACE) was performed using the SMART RACE cDNA Amplification Kit (Clontech). For LhGAP3 ...
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bioRxiv - Plant Biology 2024Quote: Rapid amplification of cDNA ends (5’ RACE) was used to determine the transcripts of the RB gene using the SMARTer RACE cDNA Amplification Kit (Clontech) according to the protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... supplemented with 10% FBS (Takara 632180). LentiX cells (Takara 632180 ...
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bioRxiv - Synthetic Biology 2024Quote: ... LentiX cells (Takara 632180) were used to produce lentivirus ...
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bioRxiv - Plant Biology 2024Quote: ... The expression vectors were then co-transformed into AH109 Saccharomyces cerevisiae (Clontech), by lithium acetate mediated transformation (Gietz and Schiestl ...
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bioRxiv - Synthetic Biology 2024Quote: ... To amplify the canvas and activity-encoding regions on the recorder plasmid construct,0 5µL of culture sample was directly used in a 10µL PCR reaction using PrimeStar Max master mix (Takara Bio, San Jose, CA) under conditions recommended by the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... The ARF sequences within pDONR207 were then recombined into the pGAD-T7 and pGBK-T7 (Clontech) expression vectors by LR clonase reactions ...
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bioRxiv - Plant Biology 2024Quote: ... and the myosin VIII and myosin XI CBD cDNAs were individually cloned into the prey vector pGADT7 (Clontech). As negative controls ...
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bioRxiv - Microbiology 2024Quote: ... Each reaction was performed in a 25-μl volume containing 12.5 μl SYBR Premix Ex Taq (TaKaRa Biotechnology, Otsu, Shiga, Japan), 0.5 μl of forward and reverse primers (10 μM) ...
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bioRxiv - Genomics 2024Quote: ... Dual-indexed primers (5μM) 2.0μL in Unique dual index kit (Takara bio) was used instead of 0.5 μL Index Primer in the default Zymo kit ...
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bioRxiv - Genomics 2024Quote: SMART-Seq Single Cell PLUS kit (Takara Bio) was used for cDNA library construction ...
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bioRxiv - Genomics 2024Quote: ... Both reagents were included in SMART-Seq Single Cell PLUS kit (Takara Bio, San Jose, CA, U.S.). Immediately after sampling in lysis buffer ...
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bioRxiv - Neuroscience 2024Quote: ... and the SMARTer Stranded Total RNA-Seq Kit v2 Pico Input Mammalian (Takara Bio USA ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by PCR with the SeqAmp PCR kit (Takara Bio USA, San Diego, CA). Unlike TARGET-Seq ...
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bioRxiv - Cancer Biology 2024Quote: ... We used the SmartScribe kit (Takara Bio USA, San Diego, CA) for RT-PCR ...
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bioRxiv - Molecular Biology 2024Quote: qPCR was performed using TB Green Fast qPCR Mix (catalog no. RR430A, TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... PrimeSTAR® Max DNA Polymerase (catalog no. R045A, TaKaRa) and ProFlex™ PCR System (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: ... and indexed using the SMARTer® RNA Unique Dual Index Kit (catalog no. 634451, TaKaRa), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... From cortical neurons subjected to OGD we generated 1) library to detect circularRNAs and mRNA and lncRNAs using SMARTer® Stranded Total RNA Sample Prep Kit (Takara Bio USA, Inc.) and 2 ...
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bioRxiv - Neuroscience 2024Quote: ... and individual cells were captured in separate wells of a 96-well plate containing 4 μl lysis buffer (1 U/μl RNase inhibitor [Clontech, Cat#: 2313B]), 0.1% Triton (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... A volume of 2 µl of cDNA was used as template for qPCR using SYBR Premix Ex Taq (Takara, Shiga, Japan, #RR420A). qPCR reactions were performed using an ABI 7500 system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... pHTN HaloTag® CMV-neo and pHTC HaloTag® CMV-neo were digested with restriction enzymes and subsequently ligated (Takara, cat n°6023). Primers and corresponding restriction digests can be found in Table S4 ...
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bioRxiv - Immunology 2024Quote: The full-length cDNA of mouse CCL2 (NM_011333.3) was amplified by PCR with PrimeSTAR Max DNA Polymerase (Takara Bio, #R045A) from mouse MCP-1/CCL2 cDNA ORF Clone (Sino Biological ...
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bioRxiv - Cell Biology 2024Quote: ... was replaced by the Lmna promoter using the In-Fusion HD Cloning Kit (Clontech) and designated as pLmna-mEmerald-C1 ...
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bioRxiv - Cell Biology 2024Quote: We cloned a 1.6 kb genomic region upstream from start codon of mouse Lmna as the endogenous promoter (-1407 to +249 bp from transcription start site) using PrimeSTAR HS DNA Polymerase (Takara). Primers used in this study are listed in Table S2 ...