Labshake search
Citations for Takara Bio :
751 - 800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... cells were harvested and vectors were purified using the AAVpro purification kit (cat.: 6666; Takara Bio, Kusatsu, Shiga, Japan) as per manufacturer’s instructions and stored at -80 °C until further use ...
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bioRxiv - Genetics 2024Quote: ... Taq (Takara) or Q5 (NEB) ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed as standard procedures in 10 μl reactions by TaKaRa Ex Taq ...
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bioRxiv - Microbiology 2024Quote: ... Each reaction was performed in a 25-μl volume containing 12.5 μl SYBR Premix Ex Taq (TaKaRa Biotechnology, Otsu, Shiga, Japan), 0.5 μl of forward and reverse primers (10 μM) ...
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bioRxiv - Genetics 2024Quote: ... PCR amplifications were done in duplicates using the PrimerSTAR GXL polymerase (Takara Bio) and 250 ng of genomic DNA per reaction ...
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bioRxiv - Genetics 2024Quote: ... Real-time PCR was carried out using SYBR Premix Ex TaqTM II (Takara) on the Applied Biosystems StepOne Real-Time PCR System ...
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bioRxiv - Genetics 2024Quote: ... then they were sequenced directly or cloned into the pMD18-T Vector (TaKaRa) for sequencing.
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bioRxiv - Genetics 2024Quote: ... PCR was performed as standard procedures in 50 μl reactions by TaKaRa LA Taq ...
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bioRxiv - Developmental Biology 2024Quote: ... Two targeting plasmids were constructed with InFusion cloning (Takara Bio), one consisting of 5’ and 3’ homology arms (each 1kb in length) ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were stained with anti-mouse E-cadherin monoclonal antibody ECCD-2 (1:100, Takara) for 16 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: RNA extraction was carried out using RNAiso Plus (TaKaRa, Dalian, China) following the manufacturer’s instructions with some modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... the larvae were embedded in agarose gel (5805A, Takara) in E3 buffer supplemented with anesthesia and N-phenylthiourea and observed using a TE2000 microscope (Nikon ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we made a master mix using the primers 2550F = 5’-GTT ACT GAT TCG TCT ACG AGA-3’ and 2718R = 5’-ATT GAA ATG ATC CAG TGC TTG-3’ and TaKaRa ExTaq DNA Polymerase (Takara Bio USA, Inc). We ran the samples for 31 cycles and ran the PCR product on a 2% agarose gel.
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bioRxiv - Evolutionary Biology 2024Quote: ... rapid amplification of cDNA ends (RACE) was performed using the SMART RACE cDNA Amplification Kit (Clontech). For LhGAP3 ...
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bioRxiv - Molecular Biology 2024Quote: qPCR was performed using TB Green Fast qPCR Mix (catalog no. RR430A, TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... PrimeSTAR® Max DNA Polymerase (catalog no. R045A, TaKaRa) and ProFlex™ PCR System (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: ... and indexed using the SMARTer® RNA Unique Dual Index Kit (catalog no. 634451, TaKaRa), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... The annealed fragment was ligated into NheI/EcoRI-digested pWALIUM20 (#1472, DGRC) vector using DNA Ligation Kit
(#6023, TaKaRa). -
bioRxiv - Molecular Biology 2024Quote: ... the coding sequences of Fasciclin 3s were PCR-amplified using 3xFLAG-tag harboring primers from Drosophila cDNA and were ligated into the BamHI/KpnI-digested pUASz1.0 vector [45] using In-Fusion (#Z9648N, TaKaRa). For all isoforms of pAc5-Fasciclin 3::3xFLAG ...
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bioRxiv - Genomics 2024Quote: ... Dual-indexed primers (5μM) 2.0μL in Unique dual index kit (Takara bio) was used instead of 0.5 μL Index Primer in the default Zymo kit ...
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bioRxiv - Genomics 2024Quote: SMART-Seq Single Cell PLUS kit (Takara Bio) was used for cDNA library construction ...
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bioRxiv - Genomics 2024Quote: ... Both reagents were included in SMART-Seq Single Cell PLUS kit (Takara Bio, San Jose, CA, U.S.). Immediately after sampling in lysis buffer ...
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bioRxiv - Neuroscience 2024Quote: ... and individual cells were captured in separate wells of a 96-well plate containing 4 μl lysis buffer (1 U/μl RNase inhibitor [Clontech, Cat#: 2313B]), 0.1% Triton (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and pLVX-E1Fα-IRES-mCherry-Puro (Takara Bio) to perform the experiments associated with its ectopic expression ...
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bioRxiv - Cell Biology 2024Quote: ... iSLK and iSLK-BAC16 cell line derivatives were generated using 2nd generation lentivirus as follows: 1×106 Lenti-X 293T cells (Clonetech/Takara via the UC Berkeley Tissue Culture Facility ...
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bioRxiv - Cell Biology 2024Quote: ... using InFusion cloning (Takara Bio). TFIIB-2xStrep was then amplified and subcloned into the Sfi1 site of a sleeping beauty plasmid pSBbi-GP (Addgene #60511 ...
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bioRxiv - Biochemistry 2024Quote: ... HRas V12-C184S] were cloned in the pEGFPC1 vector (Clontech) by restriction digestion-ligation method ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were transiently cotransfected with the expression construct pTRE3G-BI/S1PR2-LgBiT:SmBiT-ARRB2 and the expression control vector pCMV-TRE3G (Clontech, Mountain View, CA, USA) using the transfection reagent Lipo8000 (Beyotime Technology ...
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bioRxiv - Biochemistry 2024Quote: ... A gene encoding for murine full-length Arid5a (fl-Arid5a) (Eurofins Genomics) was cloned into the vector pEGFP-N1 (CLONTECH®) (Suppl ...
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bioRxiv - Biochemistry 2024Quote: ... and EeSGL1 were inserted into pCold I (Takara, Shiga, Japan) to fuse a His6-tag at the N-terminus ...
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bioRxiv - Bioengineering 2024Quote: ... the target scaffold sequence was first amplified in double-stranded DNA (dsDNA) form via PCR from M13mp18 using PrimeSTAR Max Premix (Takara Bio, R045A) and 0.8 µM primers (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... [50] The ssDNA scaffold was initially prepared using Guide-it Long ssDNA Production System v2 kit (Takara Bio, 632666) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell free extracts in a single step using immobilized affinity chromatography (IMAC) with Talon resin (ClonTech) and dialyzed overnight into 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... coli DH5α (Takara Bio Inc.) to obtain the plasmid for the linker-inserted variant.
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bioRxiv - Biochemistry 2024Quote: ... Sorted populations were collected and genomic DNA was isolated using DNAiso Reagent (Takara, 9770A). sgRNA cassettes were amplified by PCR and subjected to Next-generation sequencing (NGS ...
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bioRxiv - Biophysics 2024Quote: ... MelBSt purification from membranes by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM ...
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bioRxiv - Biochemistry 2024Quote: ... and XcSGL (KEGG locus tag, XCC2207) were amplified by PCR chain reaction using KOD plus (TOYOBO, Osaka, Japan) or PrimeSTAR Max (Takara Bio, Shiga, Japan) for the DNA polymerase and the primer pairs ...
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bioRxiv - Biochemistry 2024Quote: ... pLysS or Rosetta pLysS cells and overexpressed proteins were purified using immobilized metal-ion affinity chromatography (IMAC) with TALON resin (Clontech) (Abad et al ...
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bioRxiv - Biochemistry 2024Quote: ... Primers were designed using a PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The codons S85 and N86 were designed as the recognition sequence (TTCGAA) of BspT104 I (Takara Bio Inc., Shiga, Japan) for further modification of the linker insertion ...
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bioRxiv - Bioengineering 2024Quote: ... The resulting cDNA was utilized for analysis using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara Bio). Quantitative PCR was performed using Applied Biosystems QuantStudio™ 5 (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... DNA sequence of EB3 were obtained from pETMz mGFP-EB3 (gift from Thomas Surrey lab) were cloned by infusion (Takara Bio) into the GCN4_v4 plasmids using the primer (EB3_LZ_for ...
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bioRxiv - Bioengineering 2024Quote: ... MSCs and chondrocytes were fluorescently labeled using mitochondrial or cytoplasm targeted lentiviruses driven by an EF1α promoter for co-cultures (Takara Bio USA, Inc.). Mitochondria were labeled with GFP or mCherry (0017VCT ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid was used to generate N-terminal-truncated MGME1 (ΔN-MGME1; residues 95 to 344) by means of In-Fusion Cloning (Takara Bio). Constructs expressing the MGME1 variants used in this study were generated by site-directed mutagenesis using the pSol-His8-SUMO-MGME1 plasmid as template ...
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bioRxiv - Bioengineering 2024Quote: ... The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina) ...
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bioRxiv - Bioengineering 2024Quote: ... GFP was detected with a monoclonal mouse anti-GFP antibody (JL-8, Takara Bio USA, San Jose, CA) in Western blot analyses.
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bioRxiv - Bioengineering 2024Quote: ... The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit, Takara, 639650), and the PCR-derived regions of the resulting plasmids were confirmed by sequencing.
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bioRxiv - Biochemistry 2024Quote: ... DNaseI-treated total RNA (4 µg) was reverse transcribed using Primescript reverse transcriptase (Takara Bio) and random hexamers (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Lenti-XTM 293T (Takara, 632810) cells were transfected GFP-LC3 plasmid ...
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bioRxiv - Biochemistry 2024Quote: The Upf1-HD-His6 wild-type protein was first purified on a TALONTM resin (Clontech) equilibrated with buffer A200 ...