Labshake search
Citations for Takara Bio :
751 - 800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... For beta-catenin overexpression the CHIR99021 was substituted with 1 µg/ml doxycycline (Clontech).
-
bioRxiv - Developmental Biology 2024Quote: ... cDNA libraries were then synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech; PT4097-1) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... low-passage 3.5×106 Hek293T Lenti-X cells (632180, Takara) were seeded per 10 cm dish (Corning ...
-
bioRxiv - Developmental Biology 2024Quote: ... Hek293T Lenti-X cells (632180, Takara) were used for making lentivirus ...
-
bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed with the SYBR Premix Ex Taq kit (TaKaRa) with Rplp0 serving as the internal control ...
-
bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used were CDH1 (E-CADHERIN) (M106, Takara Biosciences) for glandular and luminal epithelial staining ...
-
bioRxiv - Developmental Biology 2024Quote: Using the Cogent NGS Analysis Pipeline software v 1.5.1 (Takara), FASTQ files containing all indices for each chip were demultiplexed to FASTQ files containing one index per file ...
-
bioRxiv - Developmental Biology 2024Quote: ... for 20 minutes on ice and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara ...
-
bioRxiv - Developmental Biology 2024Quote: ... The library for sequencing was then prepared using the SMART-Seq ICELL8 application kit (Takara) following the manufacturer’s recommended protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... the SMART-seq v4 Ultra Low Input RNA kit (Takara) was used to prepare cDNAs starting from 1.5 µg of total RNA for each sample and sequencing libraries were prepared subsequently prepared using the Ovation Ultralow System V2 (NuGen) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara) with the green and not red logic ...
-
bioRxiv - Developmental Biology 2024Quote: ... linker italics) were used to PCR amplify (Takara PrimeStar Max) the homology repair template (HRT) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Library construction started from 4.9ng of RNA per sample made using the SMARTer Stranded Total RNA-seq Kit v3-Pico Input Mammalian (Takara; 634486). The quality of libraries was checked with the with the High Sensitivity DNA Reagents Kit (Agilent ...
-
bioRxiv - Developmental Biology 2024Quote: ... anti-Ds-Red (rabbit, 632496; Clontech; 1:500), anti-mCherry (chicken ...
-
bioRxiv - Genetics 2024Quote: ... The lentivirus was concentrated using the Lenti-X concentrator (Takara Bio). The viral titer was determined by performing lentiviral transductions at varying concentrations in a 48-well plate format ...
-
bioRxiv - Genetics 2024Quote: Lentivirus was produced in the Lenti-X 23T cell line (Takara Bio). Transfection was carried out with psPAX2 (Addgene #12260) ...
-
bioRxiv - Immunology 2024Quote: ... libraries were prepared using Clontech SMARTer stranded v2 Pico Mammalian kit (Takara, San Jose, CA) with total RNA (50-100ng ...
-
bioRxiv - Immunology 2024Quote: ... The DNA fragment isolated from pCR3.1-mC/EBPα by NheI/XhoI-digestion was inserted into NheI/XhoI-digested pIRES2-AcGFP1 (Clontech, Takara) to generate pIRES2-AcGFP1-mC/EBPα.
-
bioRxiv - Immunology 2024Quote: ... The DNA fragment isolated from pCR3.1-mC/EBPα by NheI/XhoI-digestion was inserted into NheI/XhoI-digested pIRES2-AcGFP1 (Clontech, Takara) to generate pIRES2-AcGFP1-mC/EBPα.
-
bioRxiv - Immunology 2024Quote: ... The amplified DNA fragments were ligated into T vector pMD19 (#3271, Takara), and whose nucleotide sequences in each plasmid clones were determined.
-
bioRxiv - Genetics 2024Quote: ... PCR was conducted as described earlier (section: RNAi-mediated knockdown of Pmtra-2) but using the proofreading PrimeSTAR HS DNA Polymerase (Takara Bio, Shiga, Japan) and Ex Taq DNA Polymerase (Takara Bio ...
-
bioRxiv - Genetics 2024Quote: ... and Ex Taq DNA Polymerase (Takara Bio, Shiga, Japan) respectively in the 1st- and 2nd-round PCR ...
-
bioRxiv - Immunology 2024Quote: ... banked cell pellets were rapidly thawed and processed using the Nucleospin® Blood XL kit (Takara) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... and Ex Taq polymerase (Takara) per the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.
-
bioRxiv - Genomics 2024Quote: ... were digested and cloned using an Infusion master mix (Takara) upstream of the luciferase gene ...
-
bioRxiv - Genomics 2024Quote: ... Library preparation and HiFi genome sequencing were performed by Takara Bio (Shiga ...
-
bioRxiv - Genomics 2024Quote: Illumina sequencing libraries are prepared by incorporating 1-10 ng of cfDNA with the ThruPLEX® Plasma-seq Kit by Takara Bio ...
-
bioRxiv - Genomics 2024Quote: ... except that an alternative oligo-dT primer from the SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc.), which also included a random 10mer sequence as a unique molecule identifier (UMI ...
-
bioRxiv - Genomics 2024Quote: ... and concentrated to 50x in 1x PBS using Lenti-X Concentrator (Clontech, 631232) in accordance with the manufacturer’s protocol ...
-
bioRxiv - Genomics 2024Quote: ... then concentrated to 50x in 1x PBS using Lenti-X Concentrator (Takara, 631232), in accordance with the manufacturer’s protocol ...
-
bioRxiv - Genomics 2024Quote: ... Virus was concentrated using Lenti-X Concentrator (Takara) and titer quantified using p24 ELISA antigen assay (Takara) ...
-
bioRxiv - Genetics 2024Quote: ... its antibody was removed by stripping buffer (Takara Bio #T7135A) and re-blotted by PKcs antibody to quantify the total amount of DNA-PKcs (See Figures S2I-L).
-
bioRxiv - Immunology 2024Quote: ... 1 ng RNA was used as input to generate cDNA with the SMART-Seq v4 Ultra Low Input RNA Kit (Takara), according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The isolated RNA was RNase III fragmented and adaptor-ligated using PrepX mRNA Library kit (Takara, Mountain View CA), then converted into cDNA using Superscript III reverse transcriptase (Lifetech ...
-
bioRxiv - Genomics 2024Quote: ... The PCR reaction mix consisted of 1.25 PrimeSTAR GXL DNA Polymerase (Takara Bio USA, San Jose, CA), 1× Buffer ...
-
bioRxiv - Genomics 2024Quote: ... followed by an Amicon filter (MilliporeSigma, Burlington, MA) buffer exchange (Vaillancourt et al., 2019) or Takara NucleoBond HMW DNA kit (Takara, Kusatsu, Shiga, Japan). Genome assembly used hifiasm v0.16.1-r375 (Cheng et al ...
-
bioRxiv - Genomics 2024Quote: ... Lysates were incubated with His60 Ni Superflow resin (Takara Bio) at 4°C with rotation for 1 hour ...
-
bioRxiv - Genomics 2024Quote: ... were then synthesised by GenScript and initially cloned into pLVX-IRES-ZsGreen1 lentiviral vector (Takara Bio) in frame with an IRES-ZsGreen1 sequence ...
-
bioRxiv - Genomics 2024Quote: We applied three distinct cDNA library enrichment protocols for the RNA sample extracted from the same mice individual: i) standard PacBio Clontech SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc.); ii ...
-
bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-DsRED (1:1000; Clontech, Cat # 632496), chicken anti-GFP (1:250 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 3.3 mL of Lenti-X concentrator (Takara, cat.# 631231) were added to 10 mL of filtered SN ...
-
bioRxiv - Developmental Biology 2024Quote: ... libraries were prepared by the iGE3 Genomic Platform using the SMART-Seq v4 kit (Clontech, 634893) for the reverse transcription and cDNA amplification ...
-
bioRxiv - Developmental Biology 2024Quote: ... The purified PCR fragments were subsequently cloned into the XbaI-linearized pUASp-K10-attB vector (Koch et al., 2009) via In-Fusion reaction (#639648, Takara Bio, Japan). Plasmids confirmed to be correct were injected into embryos of nos-phiC31;;P{CaryP} attP2 (#25710 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The CDs of cdon was amplified using PCR (Prim STAR Max Premix Takara NO.R045A) and cloned into the vector PCS2+ to generate the expression constructs (5x In-Fusion HD Enzyme Premix ...
-
bioRxiv - Developmental Biology 2024Quote: We used In-Fusion enzyme (Takara Bio, #638947) to create a single nucleotide substitution on the 69th amino acid (Leucine to Stop ...
-
bioRxiv - Cell Biology 2024Quote: ... 1999) with a retroviral vector expressing integrin β1 under a tet-inducible promotor (Retro-X™ Tet-On®; Clontech, USA). Integrin β1-induced and FACS sorted cells were cultured in DMEM with 10% fetal bovine serum ...
-
bioRxiv - Cell Biology 2024Quote: ... Human embryonic kidney (HEK) cell lines (Lenti-X 293T) (Takara) were cultured in high-glucose DMEM (Gibco ...
-
bioRxiv - Cell Biology 2024Quote: ... Virus was concentrated with polyethylene glycol (Lenti-X, Takara, #631232). Lentivirus was produced in airway epithelial cell expansion medium supplemented with Y27632 and retinoic acid as described95 ...
-
bioRxiv - Cell Biology 2024Quote: ... and human adult heart RNA (n=4 pooled, Cat #636583, Takara) were used for bulk RNA sequencing ...