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Citations for Takara Bio :
8351 - 8400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Sequencing libraries were prepared from total RNA using the Smarter Stranded Total RNA-seq Kit v2 Pico Input Mammalian (Takara, Japan), followed by size distribution analysis in the Agilent 2100 Bioanalyzer using the High Sensitivity DNA Kit (Agilent) ...
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bioRxiv - Cell Biology 2022Quote: ... 2019) and 10 fmol JF646-LANA (see below) in 0.5 nL phosphate-buffered saline (PBS, Takara, T900) containing 0.05% phenol red (SIGMA ...
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bioRxiv - Cell Biology 2022Quote: HeLa cells stably expressing GFP-SNX32 from an inducible promoter were established following the distributor protocol (Takara Bio Inc.). GFP-SNX32 full-length gene was cloned onto pLVX-TRE3G Vector ...
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bioRxiv - Cell Biology 2022Quote: ... pLVX TRE 3G and pLVX EF1Į Tet3G were part of Tet-On® 3G Inducible Expression Systems (631363, Takara Bio Inc.). pCMV6-Entry Myc DDK CD147 (BSG ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated in media containing 200 μM of A/C Heterodimerizer (Takara) or ethanol control for 4h prior to lysis ...
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bioRxiv - Cell Biology 2022Quote: ... or mCherry-N1 backbones (Clontech). The REEP4-mCherry plasmid was constructed by inserting a codon-optimized human REEP4 gblock into mCherry-N1 using Gibson assembly ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% FBS (fetal bovine serum, Clontech) and 1% P/S (Penicillin-Streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... pDsRed2-Mito was from Clontech. pUAST-T7-DMiro-KK was generated by introducing E234K ...
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bioRxiv - Cell Biology 2022Quote: ... or by In-Fusion cloning (Takara). FRB-BFP control and FRB-Ubiquitin-BFP constructs were generated by fragment synthesis and cloned into the retroviral expression vector pQCXIH ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated using Lenti-X concentrator (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-Vti1a and GFP-DNAJC5 were generated by amplifying the corresponding sequences from a HUVEC cDNA library and cloned each into pEGFP-C1 vector (Clontech); both were provided by Johannes Nass (University of Münster ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with Lenti-X solution (Takara) and concentrated by centrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... was transfected into Lenti-X 293T cells (Takara) with pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining HRV-3C Protease was removed by TALON Metal Affinity Resin (Takara). In the rescue assays ...
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bioRxiv - Cell Biology 2022Quote: ... and the viruses were purified using Adeno-X maxi purification kits (Takara, Cat# 631533) based on the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Viral particles were precipitated using Lenti-X maxi-purification kit (Takara, Cat# 631233) based on manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The final BAC was purified with Nucleobond BAC 100 kit (Clontech) and co-injected with 50 ng/μl Tol2 RNA into one-cell-stage zebrafish embryos ...
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bioRxiv - Developmental Biology 2022Quote: ... and PrimeScript™ RT Master Mix (Takara, RR036), respectively ...
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bioRxiv - Developmental Biology 2022Quote: ... the following antibodies were used: Rx (rabbit; TaKaRa, #M228; 1:1,000), Nkx2.1 (rabbit ...
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bioRxiv - Genomics 2022Quote: ... Digested samples were column purified using the NucleoSpin Gel and PCR Clean-Up kit (TaKaRa 740609) according to the manufacturer’s directions ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was used as a template for quantitative PCR analysis on a Thermal Cycler Dice Real Time System (TaKaRa) using KAPA SYBR Fast qPCR Kit (KAPA Biosystems ...
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bioRxiv - Genomics 2022Quote: Whole bladder protein lysate was obtained by homogenizing bladder tissue using Biomasher II (TaKaRa) in ice-cold RIPA buffer with the protease inhibitor (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... Tissues were homogenized using BioMasher II (TaKaRa) with RLT Plus buffer (1% β-mercaptoethanol).
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bioRxiv - Genomics 2022Quote: ... 4 mL of lung tissue RNA (Takara Bio, 636524) at a final concentration of 500 pg per μl was used ...
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bioRxiv - Developmental Biology 2022Quote: ... Real-time quantitative PCR was performed using a SYBR Premix Ex Taq kit (Takara RR420A) in the Applied Biosystems 7500 Real-Time PCR System ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5 U Taq DNA polymerase (Takara), and 0.2 mM of dNTPs ...
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bioRxiv - Developmental Biology 2022Quote: ... EmeraldAmp GT PCR Master Mix (Takara Bio USA, Cat# RR310A) and gene specific primers (Supplementary Table 7 ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies and concentrations used: mouse anti-GFP Living Colors (JL-8) (Takara Biosciences; cat# 632381) 1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... PGDM1400 heavy and light chain sequences were synthesized (Integrated DNA Technologies) and cloned into a mammalian expression vector under a CMV promoter using InFusion (Takara) and sequence verified.
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bioRxiv - Immunology 2022Quote: ... 3,3’-diaminobenzidine (DAB) substrate (Takara, Japan) was added ...
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bioRxiv - Immunology 2022Quote: ... All resulting RNA was used as an input for complementary DNA synthesis using the SMART-Seq v4 kit (Takara Bio) and 10 cycles of PCR amplification ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared using the SMARTer® Stranded Total RNA-seq Kit v2-Pico Input Mammalian kit (Takara). Two technical replicates were generated for each experiment ...
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bioRxiv - Genomics 2022Quote: ... using the Infusion assembly protocol (TaKaRa Bio). The sequence of the resulting guide gRNA plasmid was confirmed by sequencing with either T7 or SP6 primers ...
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bioRxiv - Genomics 2022Quote: ... For PrimeSTAR GXL Premix (2x, Takara Bio, cat# R051A, Japan), a 50 μl -reaction system was prepared as follows ...
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bioRxiv - Genomics 2022Quote: ... 40U/mL of RNAse inhibitor (Takara: 2313B)] ...
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bioRxiv - Genomics 2022Quote: ... PCR was performed to generate double-stranded cDNA using PrimeSTAR GXL DNA polymerase (Takara). Sequencing libraries of both species were then constructed using SMRTbell Express Template Prep Kit 2.0 and Sequel Binding Kit 3.0 (Pacific Biosciences ...
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bioRxiv - Genomics 2022Quote: ... The SMARTer PCR cDNA Synthesis Kit (Takara) was used for first-strand cDNA synthesis ...
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bioRxiv - Immunology 2022Quote: ... TCR α- and β-chain genes were reverse transcribed and amplified from the RNA using the SMARTer RACE Kit (Clontech Laboratories, Inc). The amplified TCR genes were sub-cloned into the pEF- 1α/pENTR vector (Addgene ...
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bioRxiv - Immunology 2022Quote: ... Library preparation was performed using the ClonTech SMARTer Stranded RNA-SEq Kit V2 for mammalian pico RNA input (Takara) and RNA sequencing was performed using HiSeq 2500 sequencer with 50bp read lengths (Illumina).
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bioRxiv - Genomics 2022Quote: ... and first-strand cDNA was synthesized using a PrimeScript RT Reagent Kit (Catalog No. RR037A, TaKaRa, Tokyo), which was used for subsequent transcriptomic analyses ...
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bioRxiv - Genomics 2022Quote: ... using TB Green Premix Ex Taq II (Catalog No. RR820A, Takara, Tokyo). Relative gene expression was calculated by the 2-ΔΔCt method using the housekeeping gene translation elongation factor 1-α (EF1A ...
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bioRxiv - Genomics 2022Quote: ... First strand cDNA synthesis was performed on mRNA fragments with SMARTscribe™ M-MLV Reverse Transcriptase (Takara Bio Inc.). Illumina barcode PCR reactions were performed to prepare the cDNA for Illumina 75 bp paired-end sequencing ...
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bioRxiv - Genomics 2022Quote: ... by InFusion cloning (Takara). Next ...
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bioRxiv - Genomics 2022Quote: ... and RNA was extracted by using Trizol Reagent (Takara, Shanghai, China) according to the manufacturer’s instructions provided with the kit.
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bioRxiv - Genomics 2022Quote: ... small intestine (cat. no. 636539, Takara Bio USA, Inc), and mammary gland (cat ...
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bioRxiv - Genomics 2022Quote: ... The cell pellet was washed and rehydrated in DPBS with 0.01% BSA and 0.2 U/µl of RNase inhibitor (Takara Bio, #2313A) and 1 mM DTT (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... Reverse transcription was performed by PrimeScript™ RT Master Mix (TaKaRa). cDNA was amplified using iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: ... Total RNA pools from human brain (cat. no. 636530, Takara Bio USA, Inc), small intestine (cat ...
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bioRxiv - Genomics 2022Quote: ... and mammary gland (cat. no. 636576, Takara Bio USA, Inc) were also used for the assessment of HSAT1 expression in different tissues.
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bioRxiv - Genomics 2022Quote: ... frozen samples were triturated in a disposable homogenizer (Biomasher, Takara) with 0.1X Lysis Buffer ...