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Citations for Takara Bio :
8401 - 8450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... Isolated RNA (500 ng) was reverse-transcribed to cDNA by using PrimeScript RT master mix (Takara). PCR was performed using Mx3005P (Agilent ...
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bioRxiv - Genomics 2022Quote: The expression of each erbb3b gene and the two housekeeping genes (rpl7 and rpl32) was obtained by RT-qPCR at 65 °C (PrimeScript transcriptase, Takara, SYBRgreen) and normalized with the Pfaffle equation (Ståhlberg et al. ...
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bioRxiv - Genomics 2022Quote: ... Extracted RNA from each juvenile (as described in Methods S1) was converted to cDNA using PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan). The efficiency and specificity of the designed primers was tested through PCR using the GoTaq Green Master kit (Promega ...
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bioRxiv - Genomics 2022Quote: Sequencing libraries were prepared using 1-10 ng of cfDNA and the ThruPLEX® Plasma-seq Kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... and 2 µl of the reaction was transformed into 25 µl of Stellar chemically competent bacteria (Takara). Plasmid minipreps were performed using the NucleoSpin Plasmid Transfection-grade Mini kit (Macherey-Nagel ...
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bioRxiv - Immunology 2022Quote: ... Retrovirus vectors carrying shRNA sequences were constructed by inserting annealed oligonucleotides into a pSIN-siU6 vector (Takara) between BamHI and ClaI sites ...
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bioRxiv - Genomics 2022Quote: ... using SYBR@ Premix Ex TaqTM (TaKaRa, Japan) with β-actin as the internal control gene [10] ...
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bioRxiv - Immunology 2022Quote: ... The assembled full-length inserts were gel purified (Takara), digested with EcoRI (NEB) ...
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bioRxiv - Immunology 2022Quote: ... and cloned in BsmBI-digested backbone using Infusion HD cloning (Takara). Bacteria were plated on LB-agar containing 100ug/ml carbenicillin (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: ... Pooled libraries were subjected to maxipreps (Nucleobond Maxiprep EF kit, Takara).
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bioRxiv - Immunology 2022Quote: ... into 96-well plates containing 2 µL Lysis Buffer (Clontech) supplemented with 1 U/μL RNase inhibitor (NEB) ...
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bioRxiv - Immunology 2022Quote: ... LentiX-293T cells (Clontech, 632180) were seeded at ~60% confluence in antibiotics free media 12–16 h before transfection ...
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bioRxiv - Immunology 2022Quote: Illumina Compatible low input mRNA libraries were prepared using the Smart-Seq V4 Ultra Low Input RNA kit (Takara Bio, USA) and KAPA HyperPlus Library Preparation kit (Roche) ...
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bioRxiv - Immunology 2022Quote: ... The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio) was used to generate cDNAs and quality was assessed using an Agilent 2100 bioanalyser ...
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bioRxiv - Genomics 2022Quote: ... We used the PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa, Japan) to synthesize first-strand cDNA from 2.5 μg of total RNA ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA was extracted using NucleoSpin Blood XL (Clontech) and the shRNA sequence was amplified as previously described50 ...
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bioRxiv - Genetics 2022Quote: FIREWACh RE In-Fusion cloning (Takara) was carried out in a 2:1 insert/vector ratio ...
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bioRxiv - Genetics 2022Quote: ... Expression of CRISPRa-SunTag system was induced using doxycycline (Clontech, 1 μg/ml) one day before differentiation and kept throughout the rest of the protocol ...
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bioRxiv - Genetics 2022Quote: ... 0.1-0.2*106 mESCs were seeded per well in a 24-12-well plate in conventional ESC medium and transduced the next day with 0.25-0.5 ml of 10:1 concentrated (lenti-X, Clontech) supernatant with 8 ng/μl polybrene (Sigma Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... Takara LA Taq Polymerase (Takara RR002M) was used for PCR validation ...
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bioRxiv - Genomics 2022Quote: The genomic DNA of Mir-0 accession (ID 8337) was used as a template (2 ng) for amplification using PrimeSTAR® GXL DNA Polymerase (TaKaRa), in a 40-µl PCR reaction with 0.3 µM primers OP009 and OP010 ...
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bioRxiv - Genomics 2022Quote: Genomic DNA samples (5 ng) were used as templates in 20 μl reactions performed with PrimeSTAR GXL DNA Polymerase (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... version 2 (Takara). Fastq files were generated from bcl files using BCL2FASTQ v2.17.1.14 with default parameters ...
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bioRxiv - Immunology 2022Quote: ... Transduction was then performed per manufacturer’s instructions utilizing retronectin coated plates (Takara). Cells were removed from Dynabeads by magnetic separation on Day 5 post-stimulation and expanded in AIM-V media with 5% SR containing IL-2 at 100lU/ml ...
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bioRxiv - Immunology 2022Quote: ... TCR coding sequences were cloned into lentiviral backbones using InFusion HD (Takara), with oligonucleotides synthesized by IDT ...
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bioRxiv - Immunology 2022Quote: ... Lentivirus containing sgRNA-pLGP was generated by transfecting Lenti-X 293T cells (Takara Bio) with sgRNA-pLGP ...
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... with SYBR® Premix EX TaqTM II (Takara) and primers listed in the table (99-112) ...
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bioRxiv - Genomics 2022Quote: ... in which 5 pmol of substrate primer and 12,5 µl of TB Green Premix Ex Taq II (Takara) were added ...
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bioRxiv - Genomics 2022Quote: ... MflI (Takara, cat # 1070A) was used with 10 U in the 50 μl-reaction system containing buffer L ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The trimeric HAs were purified from the supernatant using TALON metal affinity resin (Takara, #635653), washing with PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Fabs were purified by cobalt chromatography (Takara) and further purified over an S200 column on an AKTA pure (Cytiva) ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was with the SMART-Seq v4 Ultra Low Input Kit (Takara Bio) with PolyA Selection ...
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bioRxiv - Genomics 2022Quote: ... we employed SMARTer RNA unique dual index kit -96 U (Takara Bio, 634452). SMARTer RNA unique dual index of each 5’ and 3’ PCR primer were added to each sample to distinguish pooled libraries from each other ...
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bioRxiv - Genomics 2022Quote: We prepared stranded RNA-Seq libraries for each plasma fraction using Clontech SMARTer stranded total RNA-seq kit v2-pico input mammalian (Takara Bio, 634414) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... TaKaRa LA Taq® DNA Polymerase (Takara Bio Inc., Shiga, Japan), which is optimized for long-range PCR up to >15 kb fragments ...
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bioRxiv - Genetics 2022Quote: ... The two gBlocks were added to the w+attB vector by InFusion cloning (Clontech/Takara) into the HindIII site ...
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bioRxiv - Genetics 2022Quote: ... cDNA was synthesized using Prime Script II (TAKARA) following the supplier’s protocol ...
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bioRxiv - Genetics 2022Quote: ... RT-qPCR was performed using TB Green Premix ExTaq II (Tli RNAseH Plus) (TAKARA) with Thermal Cycler Dice ® Real Time System III (one cycle of 95°C for 30 s followed by 40 cycles of 95 °C for 5 s and 60 °C for 30 s) ...
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bioRxiv - Genetics 2022Quote: ... the Oligotex-dt30
mRNA purification kit (TAKARA) was used for poly(A ... -
bioRxiv - Genetics 2022Quote: ... Extraction of high-molecular-weight DNAs was performed using NucleoBond HMW DNA (TAKARA). Library preparation was performed using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Genetics 2022Quote: ... color change was measure at OD578 and β-galactosidase units were calculated as described (Yeast Protocol Handbook, Takara Bio).
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bioRxiv - Genetics 2022Quote: ... was reverse-transcribed into the first-strand cDNA in a 20 μl reaction volume using the first strand cDNA synthesis Kit (TaKaRa, Dalian, China). PCR amplification were 94℃ for 3 min ...
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bioRxiv - Genetics 2022Quote: Full length wild-type or mutant BRC-1 sequences were cloned into plasmid pBridge (Takara Bio), transformed into yeast strain Y2HGold (Takara Bio ...
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bioRxiv - Genetics 2022Quote: ... TB Green® Premix Ex Taq™ (Takara Bio, # RR420L) and LightCycler® 480 Multiwell Plate 384 (Roche ...
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bioRxiv - Genomics 2022Quote: ... treated with DNase I (Takara, Kusatsu, Japan) and stored at -80 °C ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) with 0.5-1ug of RNA and samples were sequenced on the NovaSeq (Illumina ...
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bioRxiv - Genetics 2022Quote: ... Total protein was harvested after 3 days of culture and analyzed by Western blot (mouse anti-DsRed, Clontech; rabbit anti-POU6F2 ...
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bioRxiv - Genetics 2022Quote: ... 1 μgml−1 doxcycline (TAKARA Bio) and 10−6 M dexamethasone (Sigma Aldrich).
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bioRxiv - Genetics 2022Quote: ... The SMART-seq v4 Ultra Low input RNA kit (Clontech) was used to amplify cDNA ...
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bioRxiv - Genetics 2022Quote: Y2H were performed using the Gold Yeast Two-Hybrid System according to manufacturer’s manual (Clontech). CDSs from different genes with combination of pGADT7 and pGBKT7 were co-transformed into the yeast strain AH109 ...