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Citations for Takara Bio :
8451 - 8500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... RNA was reverse transcribed using SMARTScribe Reverse Transcriptase kit (TaKaRa, 639538). cDNA was diluted according to the RNA amount ...
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bioRxiv - Genetics 2022Quote: We prepared cDNA libraries with a SMART-seq v4 Ultra Low Input RNA Kit (Takara Bio) and SQK-LSK109 (Oxford Nanopore Technologies) ...
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bioRxiv - Genetics 2022Quote: Plasmid constructs were generated using the In-Fusion HD Cloning Kit (Takara-bio). To construct pkd-2prom: ...
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bioRxiv - Genetics 2022Quote: ... The two gBlocks were added to the w+attB vector by InFusion cloning (Clontech/Takara) into the HindIII site ...
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bioRxiv - Genetics 2022Quote: ... and then purified with RNase-Free DNase I (Takara Company, China). 1% agarose gel was used to evaluate the RNA contamination and degradation ...
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bioRxiv - Genetics 2022Quote: ... total RNA extraction and separation of residual DNA contamination were excuted using Plant RNA Extraction Kit (TaKaRa MiniBEST, kyoto, Japan), and RNase-free DNase I (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR was performed with SYBR Premix Ex Taq (TaKaRa Biotech Co., Ltd., Dalian, China) on CFX96 Connect real-time PCR detection system (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2022Quote: ... The cloNAT cassette was amplified (LJ42 and LJ43) and inserted into the XhoI site in front of the Nse3 gene using the In-fusion cloning kit (Takara). A 650bp-long genomic sequence (upstream of Nse3 ...
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bioRxiv - Genetics 2022Quote: ... transformed into yeast strain Y187 (Takara Bio) and transformants were selected on medium lacking leucine ...
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bioRxiv - Genetics 2022Quote: ... transformed into yeast strain Y2HGold (Takara Bio) and transformants were selected on medium lacking tryptophan ...
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bioRxiv - Genetics 2022Quote: ... rabbit anti-DsRed express (Clontech, 1:250) and mouse anti-Bruchpilot (nc82 ...
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bioRxiv - Genetics 2022Quote: ... The viral supernatant was concentrated using Lenti-X™ Concentrator (#631232, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... a gift from the Darzacq/Tijan Lab) sequences were inserted using InFusion cloning (Takara Bio). This cloning strategy inserted a MluI restriction digest site 3’ of the CD19 stop codon and removed the MluI restriction digest site 3’ of the EGFP stop codon ...
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bioRxiv - Genomics 2022Quote: ... InFusion cloning (Takara Bio) was used to generate all plasmids ...
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bioRxiv - Genomics 2022Quote: ... and inserted with InFusion cloning (Takara Bio). VSVGmut and scFv targeting plasmids were prepared using the HiSpeed Plasmid Maxi or Plasmid Plus Midi kits (QIAGEN) ...
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bioRxiv - Genomics 2022Quote: ... 3.5-4 million Lenti-X cells (Takara Bio) were seeded into 10 cm tissue culture dishes (Corning ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted using the Universal Genomic DNA extraction kit (Takara, Kusatsu, Japan) according to the supplier protocol and stored at -20 °C ...
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bioRxiv - Genomics 2022Quote: ... and concentrated with Lenti-X Concentrator (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... using Xfect (Takara Bio) into HEK-293T cells (CRL-2316 ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral particles were concentrated from clarified supernatants using Lenti-X Concentrator (Takara Bio). RAW 264.7 cells were transduced with the lentiviral particles and then selected with 4 μg/mL puromycin (Gibco) ...
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RTEL-1 and DNA polymerase theta promote subtelomeric DNA synthesis and telomere fusion in C. elegansbioRxiv - Genetics 2022Quote: PCR for the ITS-DNA synthesis assay was carried out using ExTaq polymerase (Takara Bio cat# RR001A, Kasatsu, Japan). 35 cycles were executed with 64°C annealing temperature and 1 minute extension time ...
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bioRxiv - Immunology 2022Quote: ... expression was induced by adding 1 μg/ml anhydrotetracycline (Takara) overnight in cell culture media ...
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bioRxiv - Immunology 2022Quote: ... Recombinant adenovirus vaccines were produced using the Adeno-X™ Adenoviral System 3 according to the manufacturer’s manual (Takara Korea Biomedical Inc. ...
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bioRxiv - Immunology 2022Quote: ... qRT-PCR was performed using the SYBRGreen included in the BacPAK™ qPCR titration kit (Clontech, USA) and a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Cytokine expression was determined with the SYBRGreen included in the Green Advantage qPCR Premix (Takara, Japan), using primers specific for TNF-a ...
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bioRxiv - Immunology 2022Quote: Gammaretroviral transduction of γδ T cells was carried out in RetroNectin (Takara Bio, Tokyo, Japan)–coated 24-well plates ...
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bioRxiv - Immunology 2022Quote: 150 tuft cells were sorted as CD45lo EpCAM+SigF+CD24+ directly into lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) and cDNA was generated following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... In-Fusion cloning (Takara Bio, Kusatsu, Shiga, Japan) was used to generate the CAR.PDZ with a PDZbm attached to the C-terminus of CD3ζ domain and site directed mutagenesis Q5(New England Biolabs ...
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bioRxiv - Genetics 2022Quote: ... in a 10 µL reaction volume with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara) following the recommended protocol ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral supernatants were spun onto nontreated wells plates coated with 30 μg/mL Retronectin reused up to three times (T100B, Takara), at 2000 x g for 2 hours at 32°C and replaced with activated human CD8+ T cells in fresh RPMI supplemented with 30 U/mL IL-2.
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bioRxiv - Genetics 2022Quote: The 5’end of the cloned fragment was amplified with a 5’RACE kit (Takara). The 5’RACE adaptor in the kit was used to evaluate the mRNA ...
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bioRxiv - Genetics 2022Quote: The 3’end of the cloned fragment was amplified with a 3’RACE kit (Takara). RNA from fresh soybean roots was used as the template ...
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bioRxiv - Genetics 2022Quote: AAVpro 293T cells (Clontech, 632273) and HEK293T cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Genetics 2022Quote: ... 5 units of Takara LA Taq (TaKaRa Bio USA, Inc.) and 1 μL of 100 μM PacBio universal primer ...
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bioRxiv - Genetics 2022Quote: ... pre-capture amplification was performed using the Takara LA Taq HotStart kit (TaKaRa Bio USA, Inc.) in two ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Quantitative PCR (qPCR) reactions were prepared in triplicate using TB Green® Premix Ex Taq™ II (Tli RNase H Plus) (Takara, USA) with a modified protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.5 U of EX Taq HS polymerase (Takara Bio, Inc.), and sterilized Milli-Q water ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The low-yield gwj02 sample was prepared with the ThruPLEX DNA-Seq Kit (Takara Bio USA, Inc., Mountain View, CA, USA). Sequences (2×250 bp ...
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bioRxiv - Genetics 2022Quote: ... RE79 and RE97) was PCR-amplified from BAC (RP23-11P22, RP23-423B1) or genomic DNA with overhangs for InFusion cloning (Takara). The fragments were ligated into a BamHI digested FIREWACh plasmid FpG5 (Addgene #69443 ...
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bioRxiv - Genetics 2022Quote: ... containing both commercially available human genomic DNA (100ng/µl; ClonTech, #636401) and mutant DNA from either a cell line (c.1620C>A ...
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bioRxiv - Genetics 2022Quote: ... Viral supernatant was further concentrated 10-fold using the lenti-XTM Concentrator (Takara Bio) following the manufacturer’s instructions and subsequently stored at −80 °C.
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bioRxiv - Genetics 2022Quote: ... the target fragment of GmXTH1 was amplified according to the SYBR Premix Ex TaqTM Kit (Takara, Otsu, Japan) operation instructions ...
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bioRxiv - Genetics 2022Quote: The RNA of soybean seedling roots were extracted using an RNAiso kit (Takara, Otsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA extraction with TRIZOL reagent and a high salt solution for precipitation (plant) (Takara Bio, Japan) was conducted according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli BL21(DE3) carrying the pGro7 plasmid (Takara, Shiga, Japan) for GroEL/ES chaperones co-expression48 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PrimerScript RT reagent kit with gDNA Eraser (TaKaRa, Japan) was employed to reverse transcribe the total RNA (1 μg ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was isolated using RNAiso Plus (TaKaRa, Japan). PrimerScript RT reagent kit with gDNA Eraser (TaKaRa ...
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bioRxiv - Evolutionary Biology 2022Quote: CDS sequence coding protein BmMETTL3(204-329) was cloned into the Pcold vector (TaKaRa, China). Primers were listed in Table S1 ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription for cDNA cloning was performed with PrimeScript™ II 1st Strand cDNA Synthesis kit (Takara), Gibson assembly was performed with NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription for quantitative PCR (qPCR) analyses was performed with PrimeScript RT Master Mix kit (Takara). qPCR was performed with TransStart Top Green qPCR SuperMix kit (TransGen).