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Citations for Takara Bio :
8601 - 8650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... and 0.25 U TaKaRa Ex Taq DNA polymerase (TaKaRa, Otsu, Japan). The resulting labelled probes were purified using Sephadex gel filtration (Illustra Sephadex G-50 fine DNA grade).
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted using the NucleoSpin kit (Takara Bio, Kusatsu, Shiga, Japan). Sequencing libraries were then prepared from 3 ng of CUT&RUN DNA with the Kapa HyperPrep Kit (Roche ...
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bioRxiv - Microbiology 2022Quote: The pBAD24a-based expression vectors of PG carboxypeptidases were constructed using In-Fusion cloning (Clontech, USA). The entire open reading frame of PG carboxypeptidase genes was amplified by PCR using cloning primer sets (See Table S2 ...
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bioRxiv - Microbiology 2022Quote: ... the supernatants were mixed with 100 μl of the Talon metal affinity resin (Clontech, USA) in a 1.5-ml tube ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants containing soluble target proteins were loaded into a Talon metal affinity resin (Clontech, USA). After washing three times with buffer A ...
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bioRxiv - Microbiology 2022Quote: ... 10 μl of the reaction mixture was prepared with 5 μl of SapphireAmp Fast PCR Master Mix (Takara #RR350B), 1 μl of each of forward and reverse primers (final concentration 0.2 μM) ...
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bioRxiv - Microbiology 2022Quote: ... 12.5 μl Takara EmeraldAmp Master Mix 2x (Takara Bio, Japan), 0.5 μl primers rbcL40+ and rbcL1444- (0.2 μM final concentration) ...
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bioRxiv - Microbiology 2022Quote: ... Stellar™ chemically competent Escherichia coli (Takara) were used to produce constructs for ectopic expression in mammalian cells ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA (cDNA) was synthesized using the PrimeScript RT reagent kit (Perfect Real Time Kit, Takara Biochemicals, China). According to the vendor’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... using the In-fusion HD Cloning Plus kit (TaKaRa) to generate plasmid pET30c-zccR ...
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bioRxiv - Microbiology 2022Quote: ... disposables and equipment were cleaned with DNA-OFF™ (Takara Bio, Inc.) and/or UV sterilized as appropriate [128] prior to amplification ...
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bioRxiv - Microbiology 2022Quote: RT-PCR was performed in a single closed tube using a one-step RT-PCR kit (One Step PrimeScript III RT-qPCR Mix, with UNG; Takara Bio Inc., Kusatsu, Japan) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR analysis was performed using TB Green Fast qPCR Mix (Takara) and Thermal Cycler Dice Real Time System II (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... and Thermal Cycler Dice Real Time System II (Takara). All primers used in this study are listed in Table S5 (No ...
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bioRxiv - Microbiology 2022Quote: cDNA was synthesized from total RNA or immunoprecipitated RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara). RT-qPCR analysis was performed using TB Green Fast qPCR Mix (Takara ...
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bioRxiv - Microbiology 2022Quote: ... The amplified fragment was cloned into the BamHI site of pET-21a(+) using In-Fusion HD cloning Kit (Takara Bio), and the NotI fragment of the resulting plasmid was then inserted in pJB861 to generate pJBacv ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, Inc.). PCR was performed with the cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... GFP expression was verified using immunoblotting using GFP (JL-8-Clontech) and α-tubulin (CP06 Calbiochem ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were first lysed in ice-cold lysis buffer (1×PBS, 0.5% sodium deoxycholate, 0.1% SDS, 0.5% NP40) with RNase inhibitor (Takara, 2313) and a protease inhibitor (Solarbio ...
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bioRxiv - Molecular Biology 2022Quote: ... was purchased from Eurofins Genomics and inserted in the C-termini of GFP with the In-Fusion HD cloning kit (Takara Bio USA). All primers used for the plasmid construction are listed in Table 3.
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bioRxiv - Molecular Biology 2022Quote: ... The full-length TLN1 was inserted into a pEGFP-N2 vector using the In-Fusion HD cloning kit (Takara Bio USA). Silent mutations on TLN1 were generated with the QuikChange site-directed mutagenesis kit (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with antibiotics as above except using tet-approved FBS (Clontech Labs 631106) at 3%.
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bioRxiv - Microbiology 2022Quote: ... CydDC was purified via His6-tag affinity chromatography using a Talon (Co-IMAC) affinity matrix (Takara Bio Inc.). For column conditioning and washing step I (10 column volumes [CV] ...
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bioRxiv - Microbiology 2022Quote: ... The amplified 0.5 kbp fragment was inserted into the BamHI site of pTK2051 using the In-Fusion Snap Assembly kit (Takara Bio USA, Mountain View, CA, USA). The resulting suicide plasmid was introduced into B ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was reverse transcribed into 10 μL of complementary DNA (cDNA) with PrimeScriptTM RT Master Mix (Takara Biomedical Technology, China) at 37°C for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: ... a DNA fragment of AT1G13390 was amplified from Col-0 genomic DNA using CloneAmp HiFi PCR Premix (Clontech) with primers listed in Table S7 ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were further introduced into the XmnI and KpnI digested pB7FWG2.0 with In-Fusion (Clontech). For dual-luciferase analysis ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-RFP (Takara, 632543) primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... was used for oligo(dT)18-primed cDNA synthesis according to the reverse transcription protocol (TaKaRa). The resulting cDNA was subjected to relative quantitative PCR using the SYBR Premix Ex Taq kit (TaKaRa ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was subjected to relative quantitative PCR using the SYBR Premix Ex Taq kit (TaKaRa) on a Roche LightCycler 480 real-time PCR machine ...
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bioRxiv - Molecular Biology 2022Quote: ... Vectors were transformed into yeast strain Y2HGold (Clontech, Japan). Yeast transformation was performed according to the Yeastmaker Yeast Transformation System 2 User Manual (Clontech ...
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bioRxiv - Molecular Biology 2022Quote: ... Yeast transformation was performed according to the Yeastmaker Yeast Transformation System 2 User Manual (Clontech, Japan). The primers used are listed in Supplemental Table S1.
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bioRxiv - Molecular Biology 2022Quote: ... RNA samples were reverse transcribed using an RT Primer Mix (oligo dT) and PrimeScript RT Enzyme Mix for qPCR (TaKaRa, Japan) following the manufacturer's protocol ...
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bioRxiv - Molecular Biology 2022Quote: EGFP and TDP-43EGFP cDNAS were amplified from pEGFP-C1 (Clontech, 6084-1) and pEGFP-C1-TDP-43 (kind gift from Dr ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% tetracycline-free FBS (Takara Bio, 631106), 1% Penicillin/Streptomycin and 2μg/ml blasticidin ...
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bioRxiv - Microbiology 2022Quote: ... LA Taq (RR002A, TaKaRa) or PrimeSTAR MAX Premix (R045 ...
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bioRxiv - Microbiology 2022Quote: ... or PrimeSTAR MAX Premix (R045, TaKaRa) was used for the generation of PCR products ...
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bioRxiv - Microbiology 2022Quote: ... These PCR products were then assembled and cloned into a plasmid pAPT110 linearized with NheI using the In-Fusion® HD cloning kit (Takara Bio) to generate plasmid p4G39 ...
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bioRxiv - Microbiology 2022Quote: ... Restriction endonucleases and T4 DNA ligase were purchased from Thermo Fisher Scientific and PrimeSTAR DNA polymerase from Takara Bio ...
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bioRxiv - Immunology 2022Quote: Doxycycline (Dox)-inducible gene-expressing cell lines were generated using Retro-X™ Tet-On 3G Inducible Expression System (Takara Bio). Plat-GP cells were transfected with the pRetroX-Tet3G plasmid together with the packaging plasmid pCMV-VSV-G using Lipofectamine 2000 ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription was performed using PrimeScriptTM RT Reagent Kit (Takara Bio Inc., Japan) with gDNA Eraser ...
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bioRxiv - Immunology 2022Quote: ... as well as with retroviral particles with RetroNectin® (Takara) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with a 1.5:1.5:1 μg ratio of the SFG:Peq-Pam:RD114 DNA plasmids and concentrated using Retro-X™ Concentrator (Takara, Kusatsu, Japan) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Infections of fully differentiated MDM were performed after adherent growth in the presence of M-CSF for seven days ...
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bioRxiv - Microbiology 2022Quote: ... 1 μg total RNA was reverse-transcribed using PrimeScript RT reagent Kit (RR047A, TaKaRa). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... The coding sequence of enhanced green fluorescent protein (eGFP) was amplified from plasmid eGFP-C1 (6084-1, Clontech) using primers eGFPN-F/eGFPN-R ...
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bioRxiv - Microbiology 2022Quote: ... all RNA preparations were subjected to a second DNase treatment using the genomic DNA (gDNA) Eraser (Perfect Real Time Kit, Takara Biochemicals, China). RNA concentrations were quantified on a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli DH5α (9057, Takara) and Agrobacterium tumefaciens AGL-1 (Lab stock ...
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bioRxiv - Immunology 2022Quote: ... Lentivirus particles were concentrated with Lenti-XTM Concentrator (Takara 631232) with the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... Concentrated particles were resuspended in 100% WRN conditioned media and titers were checked using Lenti-XTM GoStixTM Plus (Takara 631280). Only high titer lenti-particles were used to transduce duodenoids ...