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Citations for Takara Bio :
8551 - 8600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed with SYBR Premix Ex Taq™ II (Takara Bio Inc.) or THUNDERBIRD SYBR qPCR Mix (Toyobo ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... The TB Green Premix Ex Taq (TaKaRa, RR420A) was used in the qRT-PCR reaction ...
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bioRxiv - Plant Biology 2022Quote: ... and pGEX4T3 (Cytiva) using the In-Fusion® HD EcoDry™ kit (Takara) and ...
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bioRxiv - Plant Biology 2022Quote: ... Bulk (RR420L, Takara, Japan).
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bioRxiv - Plant Biology 2022Quote: ... or the bait vector pGBKT7g (Clontech Laboratories Inc.). The resulting plasmids were co-transformed into Y2HGold (Clontech Laboratories Inc. ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from total RNA using PrimeScript™ reverse transcriptase reagent Kit (Perfect Real Time) (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... were amplified using a set of primers with Gateway attB sites (supplementary Table S2) and cloned into the pABAi vector (Cat. No. 630491, Takara Bio USA, Inc.). The MaMYBPA1 and MaMYBPA2 coding sequences were cloned into the pGADT7-GW vector (Clontech ...
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bioRxiv - Plant Biology 2022Quote: The Matchmaker Gold Yeast 1-hybrid system (Clontech) with the Y1H gold yeast strain was used ...
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bioRxiv - Plant Biology 2022Quote: ... StNPR1 or StNPR3/4 cds) construct combinations were transformed into the Y2H Gold strain using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, USA) and the transformants selected on control SD media without Leu and Trp (-L-W) ...
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bioRxiv - Plant Biology 2022Quote: ... The MaMYBPA1 and MaMYBPA2 coding sequences were cloned into the pGADT7-GW vector (Clontech) to generate effector constructs ...
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bioRxiv - Plant Biology 2022Quote: ... PCR reaction mixture was prepared at a final volume of 20µl which contains 10 µl of TB Green Premix Ex Taq II (Takara), 10pM of forward and reverse primers and 2µl of diluted cDNA ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting plasmids were co-transformed into Y2HGold (Clontech Laboratories Inc.) and cultured at 30°C on synthetic defined (SD ...
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bioRxiv - Plant Biology 2022Quote: ... and MaMYC coding sequences were recombined into the prey vector pGADT7g (Clontech Laboratories Inc.) or the bait vector pGBKT7g (Clontech Laboratories Inc.) ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated using Quick-RNA miniprep kit (ZymoResearch, CA, USA) and 1 μg of RNA was used to generate cDNA using SMART MMLV reverse transcriptase (Takara Bio USA, Inc.). Transcript abundance of an ABA biosynthesis gene ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR analysis was performed using SYBR Premix Ex Taq II (TaKaRa) and Stepone PCR instrument ...
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bioRxiv - Plant Biology 2022Quote: ... A cDNA library constructed using switchgrass leaves’ cDNA were screened following the Matchmaker Gold Y2H system user manual (Clontech). The library screening was performed on SD/-Trp-Leu-Ade-His medium ...
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bioRxiv - Plant Biology 2022Quote: ... 5 × PrimeScript™ RT Master Mix (TAKARA) was used to synthesize cDNA ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from six different tissues using Trizol reagent (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: The PsiYAB11 and PtoYAB11PSC genes were cloned into the pGADT7-rec vector (Clontech, Mountain View, CA, USA) with the GAL4 activation domain ...
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bioRxiv - Plant Biology 2022Quote: ... StNPR1 and StNPR374 were amplified and inserted into the pGBKT7 (bait) yeast expression vector through in vivo cloning with Matchmaker Gold Yeast Two-Hybrid System (Clontech, USA), to produce proteins with an N-terminal Gal4 DNA-binding domain ...
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bioRxiv - Plant Biology 2022Quote: ... StNPR1 and StNPR3/4 were inserted also into the pGADT7 (prey) vector (Clontech, USA), to produce proteins with an N-terminal Gal4 activation domain ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using the NucleoBond Xtra BAC kit (Takara Bio 740436.25) and stored at 4°Cfor less than one week before delivering to mESCs.
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bioRxiv - Neuroscience 2022Quote: ... cDNA syntheses were generated using PrimeScript RT Master Mix (RR036A, TaKaRa). SYBR® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Neuroscience 2022Quote: ... SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (RR820A, TaKaRa) were performed with the CFX96 Touch Deep Well Real-Time PCR system under standard conditions ...
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bioRxiv - Microbiology 2022Quote: ... The resultant lysate was cleared by centrifugation at 30 000 x g for 20 min and applied to Talon CellThru Co2+ chelating resin (TaKaRa-Clontech). The protein bound resin was washed with 3 column volumes (CV ...
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bioRxiv - Microbiology 2022Quote: ... The resultant lysate was cleared by centrifugation at 30 000 x g for 20 min and applied to Talon CellThru Co2+ chelating resin (TaKaRa-Clontech). The protein bound resin was washed with 3 column volumes (CV ...
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bioRxiv - Neuroscience 2022Quote: RNA was extracted using the MiniBEST Universal RNA Extraction Kit (Cat No. 9767, TaKaRa). cDNA syntheses were generated using PrimeScript RT Master Mix (RR036A ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification with Gateway cloning compatible primers was performed directly on Aag2 gDNA using CloneAmp Hifi PCR pre-mix (Takara), without prior amplification in a TOPO TA cloning vector ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from ICP1 cells using RNAiso Plus (Takara Bio, Dalian, China) and was reverse-transcribed to cDNA using HiScript® II Q Select RT SuperMix (Vazyme ...
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bioRxiv - Molecular Biology 2022Quote: ... The full-length coding region of chicken CDKN3 (accession number: NM_001252162.1) was amplified by RT-PCR and cloned into the empty vector pCMV-HA (Clontech, San Francisco, CA, USA) to construct the overexpression plasmid pCMV-HA-CDKN3 ...
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bioRxiv - Microbiology 2022Quote: ... pseudotuberculosis were constructed using the In-fusion HD cloning kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... These fragments were cloned into pUC19 with the HYG resistance cassette upstream of pCTR4-2 with InFusion cloning (Takara, Cat#638911). Plasmids were isolated from individual clones and confirmed by digestion with HindIII (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-Up kit (Takara Bio, Kusatsu, Shiga, Japan), and 600 ng-1 ug of DNA templates were added to set up in vitro transcription reactions ...
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bioRxiv - Microbiology 2022Quote: ... Reactions containing 1x premix (Takara), 0.5μM forward primer ...
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bioRxiv - Neuroscience 2022Quote: ... or the SMARTer Stranded Total RNA Kit v2 - Pico Input Mammalian (Takara) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... digested with SacI (Takara Bio), and then ligated with Aor51HI- (Takara Bio ...
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bioRxiv - Plant Biology 2022Quote: ... and then ligated with Aor51HI- (Takara Bio) and SacI- (Takara Bio ...
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bioRxiv - Plant Biology 2022Quote: ... The total RNA was extracted from the whole plant using NucleoSpin RNA (Takara, Japan). Sequence data of LmZTL_1 (LC708291) ...
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bioRxiv - Plant Biology 2022Quote: ... or pDONR207 by in-fusion (Takara, Catalog Number 638909), then transferred into expression plasmids by LR reactions (Invitrogen ...
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bioRxiv - Physiology 2022Quote: Total RNA was extracted using NucleoSpin RNA Kit (Takara) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... SEP and DOR sequences were amplified by PCR and inserted into PCAGGS-SE using EcoRI and XhoI sites with a two fragments In-Fusion (Takara Bio) strategy ...
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bioRxiv - Physiology 2022Quote: Total mRNA of epididymal adipose tissue was extracted with Trizol (Takara Bio Inc., Japan). The concentration and purity of extracted total mRNA were measured using NANODROP ONE (Thermo Scientific ...
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bioRxiv - Physiology 2022Quote: ... and mCherry (1:500, Takara). Alexa Fluor-conjugated secondary antibodies were used at 1:500 (Millipore) ...
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bioRxiv - Physiology 2022Quote: ... Relative quantitative PCR was conducted with a TB Green Primer Ex Taq II (Takara Bio Inc., Japan) using LightCycler® 96 Real-Time PCR System (Roche ...
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bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel, Switzerland], 30% glycerol, and 1 × Tris/glycine/SDS [TG-SDS] buffer [Takara Bio Inc.]) and then electrophoresed in a running buffer (1 × TG-SDS buffer and 10 mM 2-mercaptoethanol ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized with the PrimeScript™RT reagent kit (Takara Bio Inc., Japan) following the manufacturers’ instructions ...
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bioRxiv - Physiology 2022Quote: ... The primer sequences were obtained from Takara and listed in supplementary Table 1 ...
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bioRxiv - Physiology 2022Quote: ... and 10 mM 2,3-butanedione monoxime (BDM; FUJIFILM Wako Pure Chemical Corp.) in Ca2+- and Mg2+-free phosphate-buffered saline (PBS; Takara Bio Inc., Kusatsu, Japan). The aortic valve was then ligated ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-GFP (1:200;) and rabbit anti-dsRed (1:600; Clontech). The anti-GFP and anti-dsRed antibodies were used to amplify the intrinsic fluorescent signals in the Ccr2RFP/+fmsEGFP/+ mice ...