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Citations for Takara Bio :
8501 - 8550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Lentiviral particles were produced by transfecting HEK293T cells (#632180; Clontech, Westburg, Leusden, The Netherlands) according to the manufacturer’s protocol (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: Candidate enhancer sequences identified from the integrated analysis from scRNA-seq and scATAC-seq were PCR amplified (TAKARA, PrimeSTAR GXL) from mouse genomic DNA and PCR products cloned into pGL4.23 (Promega ...
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bioRxiv - Developmental Biology 2022Quote: ... Candidate enhancers were PCR amplified (Takara PrimeSTAR GXL premix, R051A) from mouse genomic DNA using primers listed below ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and cloned into SacI- and Xho-digested pGL3-Promoter vector using the Infusion®HD Cloning kit (Takara Bio #639650). Elements were cloned upstream of the sv40 promoter within the pGL3-Promoter vector ...
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bioRxiv - Immunology 2022Quote: Brunello/Calabrese screens: Lenti-X-293T cells (Takara Bio) were plated in T-175 culture flasks (0.6×106 cells/ml ...
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bioRxiv - Immunology 2022Quote: ... Libraries were prepared using Clontech Ultra Low RNA SMARTer v4 (Takara) and sequenced on a HiSeq2000 ...
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bioRxiv - Genetics 2022Quote: ... and then yeast two-hybrid assays were performed in a Gal4-based system from Clontech in a diploid strain by mating as previously described (Rossignol et al ...
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bioRxiv - Genomics 2022Quote: ... The ligation reactions were heat-inactivated at 65°C for 10 minutes and cleaned up with NucleoMag NGS Clean-up and Size Select beads (Takara Bio) before electroporation into Endura Electrocompetent E ...
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bioRxiv - Genomics 2022Quote: ... and the bacterial lawn later scraped for plasmid extraction using NucleoBond Xtra Midi Plus (Takara Bio). The plasmid pool was then triple-digested with SphI-HF ...
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bioRxiv - Genomics 2022Quote: ... The indexed barcodes were cleaned up with NucleoSpin Gel and PCR Clean-Up (Takara Bio) and DNA concentration measured using Qubit dsDNA HS kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... The cDNA was reverse transcribed and applied for PCR/qPCR analysis with PrimeScript™ reverse transcriptase (2680B, Takara, Japan). As an internal control for normalization ...
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bioRxiv - Genomics 2022Quote: ... followed by magnetic bead purification with NucleoMag NGS Clean-up and Size Select (Takara Bio). To prepare the CRISPEY-BAR middle insert for ligation ...
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bioRxiv - Genomics 2022Quote: ... and the bacterial lawn later scraped for plasmid extraction using NucleoBond Xtra Midi Plus (Takara Bio). 20 individual bacterial colonies were separately picked from a dilution plating and miniprepped using Nucleospin Plasmid (Takara Bio) ...
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bioRxiv - Genomics 2022Quote: ... and dsRNA was purified using the NucleoSpin® RNA Cleanup XS kit (Clontech). To perform RNAi ...
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bioRxiv - Genomics 2022Quote: ... ten 50μL reverse transcription reactions were carried out using PrimeScript™ Reverse Transcriptase (Takara, #2680) and a gene specific primer (STARR_GSP ...
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bioRxiv - Plant Biology 2022Quote: ... purified with a high-salt solution for precipitation (Takara Bio Inc., Ohtsu, Japan) and reverse transcribed with ReverTra Ace (Toyobo) ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR products of stage 2 ray florets using c25599_g2_i1 Fill-F and c25599_g2_i1 Fill-R primers (Table S2) were digested with SacI (Takara Bio Inc). Reaction mixture was consisted of 2.5 µL of PCR product ...
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bioRxiv - Plant Biology 2022Quote: ... Gaillardia × grandiflora ‘Arizona Apricot’ and Antirrhinum majus using Sepasol RNA I Super G (Nacalai Tesque) and purified with a high-salt solution for precipitation (Takara Bio Inc.). A five-micro gram of total RNA was used for the gel blot analysis ...
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bioRxiv - Plant Biology 2022Quote: ... Cytosolic domain fragments of NbPMA3 (F1, F2 or F3) were fused with the Gal4 activation domain in pGADT7 (Clontech, PT3249-5, USA) and inserted into the Y187 strain under selection with SD/-Leu ...
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bioRxiv - Plant Biology 2022Quote: ... and inserted into the Saccharomyces cerevisiae Y2HGold strain (Clontech, USA) under selection with SD/-Trp ...
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bioRxiv - Plant Biology 2022Quote: CRISIS2 or Nb14-3-3 was fused with the Gal4 DNA binding domain in pGBKT7 (Clontech, PT3248-5, USA) and inserted into the Saccharomyces cerevisiae Y2HGold strain (Clontech ...
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bioRxiv - Plant Biology 2022Quote: ... The resultant NOS terminator fragment and pENTR_gMpTIR1 were digested with NotI (Takara Bio) and then ligated to generate pENTR_NosT-gMpTIR1 ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 500 ng total RNA using a Prime Script RT Reagent Kit (Takara Bio, Otsu, Japan). RT- PCR was performed using three primer sets ...
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bioRxiv - Plant Biology 2022Quote: ... followed by selection of transformants and testing of pair-wise interactions by growth complementation assays on nutrient selection media as described in the Matchmaker™ GAL4 Two-Hybrid System 3 manual (Clontech). To compensate for auto-activation of some constructs ...
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bioRxiv - Plant Biology 2022Quote: ... purified with Fast pure gel DNA extraction mini kit (DC301, Nanjing Vazyme Biotech, China) and then cloned into the pMD19-T vector (TaKaRa, Japan). The CRISPR-edited sequences were screened by Sanger sequencing of pMD19-T vector.(K ...
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bioRxiv - Plant Biology 2022Quote: ... Transferred membranes were blocked with 5% (w/v) milk for 30 mins at RT before being hybridized with the corresponding anti-GFP (632381, Takara) or anti-mCherry (632543 ...
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bioRxiv - Plant Biology 2022Quote: ... PCR products were amplified with specific primers based on the off-target sites and purified products were cloned into pMD19-T vector (TaKaRa, Japan) for Sanger sequencing ...
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bioRxiv - Plant Biology 2022Quote: Full-length CDSs of CiS40-11 was cloned into the expression vector pCanG-HA and pCAMBIA1302 using In-Fusion® HD Cloning Kit (TaKaRa, Japan). All generated binary plasmids were confirmed by enzyme digestion and sequencing validation ...
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bioRxiv - Plant Biology 2022Quote: The genomic sequence corresponding to the BnaAnng22030D gene and its surroundings was amplified using specific primers (Supplemental Table S1) and PrimeSTAR GXL DNA Polymerase (Takara). The PCR product was cloned and sequenced ...
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bioRxiv - Plant Biology 2022Quote: Yeast one-hybrid assays were performed using the Matchmaker Yeast One-Hybrid System (Clontech). The DNA fragment 1,000 bp upstream of the start codon ATG of DDRM2 was cloned into pHis2.1 vector and SOG1 was cloned into the pGADT7 vector ...
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bioRxiv - Plant Biology 2022Quote: ... The genomic DNA sequences surrounding the potential off-target sites were amplified by PCR using specific primers (Supplemental Table S1) and PrimeSTAR GXL DNA Polymerase (Takara). PCR products were analyzed by sequencing.
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bioRxiv - Plant Biology 2022Quote: ... we used the Matchmaker Gold System (Takara Bio USA) following a protocol adapted from (46 ...
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bioRxiv - Plant Biology 2022Quote: ... using the In-Fusion HD cloning kit (Clontech, Mountain View, CA) to generate pJHY- TMp1_MpTIR1 ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR products and pGEX6P-1 vector were digested with EcoRI (Takara Bio, Shiga, Japan) and NotI (Takara Bio ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed into cDNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Japan). qRT-PCR was performed with Roche LightCycler 480 Real Time PCR system (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... as described by the manufacturer’s instructions and complementary DNA was synthesized for real-time quantitative polymerase chain reaction (RT-qPCR; Primescript RT Reagent Kit, Takara Bio, Kusatsu, Japan). RT-qPCR analysis was performed for the gene PR1 (AT2G14610 ...
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bioRxiv - Plant Biology 2022Quote: ... pairwise AD and BD were co-transformed into yeast strain Y2H gold (Clontech). Cells transformed with both plasmids were selected after growth 2 d at 30□ on synthetic dropout medium lacking leucine and tryptophan ...
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bioRxiv - Plant Biology 2022Quote: ... PtoATPE and PtoPSBB were inserted into a pAbAi-BR yeast-integrating vector (Clontech), which was used as a bait-reporter construct ...
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bioRxiv - Plant Biology 2022Quote: ... then ligated by Infusion reactions (Takara 638909). A 2.7 kb region upstream of the TMK1 initiator ATG was inserted into TMK1-pENTR/D-TOPO by Infusion reaction and transferred into vector pW0211 ...
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bioRxiv - Plant Biology 2022Quote: ... benthamiana according to a standard AcLi-mediated transformation kit (Matchmaker; Clontech, Mountain View, CA, USA). To repeat the interaction ...
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bioRxiv - Plant Biology 2022Quote: ... Coding regions of CjTKPR1 and AtTKPR1 were amplified using PrimeStar GXL polymerase (Takara Bio) with specific primers (Supplementary Table 5) ...
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bioRxiv - Plant Biology 2022Quote: ... and Nos terminator (NosT) fragments were combined with In-Fusion HD mix (Takara Bio) and cloned to Escherichia coli ...
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bioRxiv - Plant Biology 2022Quote: Direct protein-protein interaction was tested by Y2H technique according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany). Yeast strain Y190 was co-transformed with respective plasmids ...
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bioRxiv - Plant Biology 2022Quote: ... using 10 pmol random nonamers (Takara) in 20 μl reaction volume and according to manufacturers’ instructions ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesised using the PrimeScript II kit (Takara Bio). Total RNA and genomic DNA from A ...
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bioRxiv - Plant Biology 2022Quote: ... thaliana using the PrimeScript IV 1st Strand cDNA synthesis kit (Takara Bio, Shiga, Japan). CjTKPR1 and EF1-α were amplified using intron-containing primers to distinguish between cDNA and gDNA amplification ...
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bioRxiv - Plant Biology 2022Quote: ... fragments were recombined into GW versions of the GAL4 DNA-binding domain vector pGBT-9 and the activation domain vector pGAD424 (Clontech). Oligonucleotides used for cloning are listed in Supplementary Table S3.
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bioRxiv - Synthetic Biology 2022Quote: ... PrimeStar DNA polymerase and SapphireAmp* Fast PCR Master Mix were purchased from TaKaRa Bio ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCRs used the PrimeSTAR GXL DNA Polymerase (Takara Bio) under conditions following the protocol listed by the supplier ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...