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Citations for Worthington Biochemical :
601 - 650 of 705 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the separated dermal tissue was cut into small pieces and incubated with 0.2% collagenase type II (Worthington Biochemical Corp. Lakewood, NJ. USA) for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Human embryonic tissues (CS12-CS13) analyzed by RNA sequencing were incubated in medium containing 0.23% w/v collagenase Type I (Worthington Biochemical Corporation, NC9482366) for 30 minutes at 37°C and the single cell suspensions were filtered through a 70 µm cell strainer (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... were isolated from ventricles of 8-month-old adult FVB hearts (Taconic, Germantown, NY) by retrograde heart perfusion with collagenase type II (Worthington Biochemical Corp). ACM were allowed to settle by gravity in the washing buffer with 2% BSA and stepwise [Ca2+] increase from 20 μM to 1.0 mM ...
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bioRxiv - Cancer Biology 2024Quote: Mechanical tissue dissociation was performed using scissors and enzymatic dissociation was performed using 1000 U/mL Type IV collagenase (Worthington, Cat: LS004188) at 37 °C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred to a 15ml tube with 8ml collagenase solution (∼4,000 units in αMEM with 1% anti-mycotic; Type IV, Worthington Biochemical Corp, Lakewood, NJ). The solution containing the tissue fragments was then continuously agitated for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... the epithelial sheet was peeled off from mesenchymal tissue in trachea with a tungsten needle after the incubation with 400U/ml collagenase type I (Worthington Biochemical Corporation, CLS1) at 37°C for 60 min or 16U/ml Dispase (Corning ...
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bioRxiv - Biochemistry 2020Quote: ... Primary myofibroblasts were disaggregated from the fresh surgical tissues using 300 units/g type II collagenase at 1mg/ml (Worthington Chemical, NJ, USA) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining frozen tissue was weighed and briefly sliced on dry ice and then incubated in 75 U/ml collagenase type 3 (Worthington #CLS-3, S8P18814) in Hibernate-E solution (human tissue for 20 min ...
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bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2020Quote: ... the epithelial sheet was peeled off from mesenchymal tissue in developing trachea with a tungsten needle after the incubation with 175U/ml collagenase type I (Worthington Biochemical Corporation, CLS1) at 37°C for 6 - 60 min ...
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bioRxiv - Paleontology 2023Quote: ... The matrix sections were then incubated overnight at 37 degrees Celsius in a solution of ∼1 mg/mL collagenase (collagenase type I, Worthington Biochemical Corporation, 55B7870) dissolved in Dulbecco’s PBS (pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: A total of 16 FDL tendons per group per age were pooled together into low glucose Dulmecco’s Modified Eagle Medium (DMEM) and subsequently digested in Collagenase Type I (5mg/ml) (Worthington Biochemical, Lakewood, NJ, LS004196) and Collagenase Type IV (1mg/ml ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2023Quote: ... Tissue fragments were incubated in 1.35 mL PBS plus 150 uL 20 mg/mL Collagenase Type I (Worthington CLS-1 dissolved in PBS) and 15 uL 20 mg/ml DNase I (Worthington D dissolved in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... spun down by centrifugation at 500 x g for 5 minutes and resuspended in 10 ml of digestion medium (MEM containing 112 U/mL type I collagenase; Worthington, cat. #CLS-1), 1.2 U/mL dispase (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Brain tissues were manually minced using razor blades and enzymatically dissociated at 37°C for 30 min with 1 mg/mL collagenase type IV (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/mL DNase 1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
Analysis of RyR2 distribution in HEK293 cells and mouse cardiac myocytes using 3D MINFLUX microscopybioRxiv - Physiology 2023Quote: ... Once fluids ran clear the perfusion was switched to an isolation buffer which included 2 mg/mL collagenase type II (Worthington Biochemical, catalog: LS004176) for 7 minutes at 2 mL/min ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Paleontology 2023Quote: ... the OBM sections were then incubated overnight at 37 degrees Celsius in 1 mg/mL collagenase (collagenase type I, Worthington Biochemical Corporation, 55B7870) with 1% sodium azide dissolved in Dulbecco’s PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... minced in F12 medium and resuspended in 10 mL of digestion medium (minimum essential media containing 112 U/mL type I collagenase; Worthington, cat. #CLS-1), 1.2 U/mL dispase (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... These tissue strips were then enzymatically digested into single-cell suspensions using a cocktail containing 3.2mg/ml collagenase Type II (Worthington Biochemical Corp, catalog #LS004176), 0.7mg/ml elastase (Worthington Biochemical Corp ...
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bioRxiv - Bioengineering 2024Quote: ... while the dermal layer was incubated in 10% FBS-supplemented DMEM containing 5000 U/ml collagenase type 1 (Worthington Biochemical Corp., NJ, USA) in a 37 °C water bath ...
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bioRxiv - Cell Biology 2024Quote: ... Each leaflet was incubated for 20 min at 37 °C in 2 mg/mL type II collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA) in cell culture medium (advanced Dulbecco’s modified Eagle’s medium (Ad DMEM ...
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bioRxiv - Immunology 2021Quote: ... Lungs were either homogenized and centrifuged to obtain clarified supernatants to assay for viral titres or enzymatically digested in collagenase III (Worthington Biochemical Corporation, USA; 1mg/ml) and DNase I (Sigma-Aldrich ...
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bioRxiv - Physiology 2020Quote: ... oocytes contained in 1-3 mL of ovary lobes extracted via survival surgery were isolated by enzymatic digestion with collagenase type II (Worthington Biochemical Corp., NJ, USA) under mechanical agitation ...
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bioRxiv - Molecular Biology 2022Quote: Calvarial osteoblasts were obtained from 3 to 5-day-old C57BL/6 mice by sequential enzyme digestion [1 mg/ml collagenase type II (Worthington Biochemical, Lakewood, NJ, USA) in Hanks’ balanced salt solution (Life Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... After 2 min this was replaced with a digestion solution: the modified Tyrode above containing 100 μM CaCl2 and either 0.6 mg/mL of collagenase (type II, Worthington Biochemical Corp., Lakewood, NJ, USA) or 0.02-0.04 mg/ml Liberase™ TH (Roche ...
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bioRxiv - Physiology 2020Quote: ... Tissue chunks were transferred into a digestion device and then subjected to sequential (6 steps) enzymatic digestion (at 37°C) using collagenase type V (Worthington Biochemical Corporation, Lakewood, NJ) 250 U/mL and protease type XXIV (Sigma-Aldrich Co. ...
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bioRxiv - Cell Biology 2022Quote: ... hearts were excised and perfused through the coronary arteries on a Langendorff system with a calcium-free physiological saline solution followed by a Ca2+/enzyme-containing saline mixture (Type II collagenase and protease XIV; Worthington and Sigma Aldrich, respectively). Single isolated cells were separated by mechanical agitation and cellular debris was removed by filtration through a nylon mesh with 250-μm porosity ...
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bioRxiv - Molecular Biology 2024Quote: ... The fatty and adventitial layers were removed through an 8–10- minute digestion with an enzyme cocktail consisting of 1 mg/ml collagenase type II (Worthington Biochemical Corp, catalog #LS004176), 1 mg/ml soybean trypsin inhibitor (Worthington Biochemical Corp ...
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bioRxiv - Microbiology 2024Quote: ... intramuscular fat of Anguilla japonica was digested with equal volume (v/w) of HBSS containing 1 mg/ml of collagenase type IV (Worthington Biochemical Corporation, NJ, USA) and 5 mM calcium chloride in 37°C for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... 2 mL of Opti-AB containing 1 μg/mL N-p-tosyl-ʟ-phenylalanine chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals, NJ, USA) was added to each well ...
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bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (3 months old) were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70µm and 40µm cell strainers (Falcon #07-201-431 and #07-201-430) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cell Biology 2024Quote: ... hindlimb muscles from mice and hamstring muscles from humans were digested with collagenase II (LS004177, Worthington, 1000 units per 1 ml) for 90 min at 37 °C ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... 0.5 mM EDTA (Worthington Biochem kit), and 0.01% DNase (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Human iPSC-CMs used for patch clamp measurements were dissociated from EHT by collagenase II (200 U/mL; Worthington Biochemical, Lakewood, NJ, USA) for 5 h ...
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bioRxiv - Neuroscience 2020Quote: ... The Papain Dissociation Kit (Cat# LK003150 Worthington) was used to dissociate the tissue ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... or a papain dissociation kit (Worthington LK003150) for less than 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Dissociation was performed using papain dissociation kit (Worthington Biochemical) for 20 minutes at 37°C and stopped using DMEM + 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... papain (PDS Kit; LK003178; Worthington Biochemical Corporation, Lakewood, NJ), DNase I (90083 ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... Processing was done using the Papain kit from Worthington, and cells were counted and diluted to a density of 5 Million per ml ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi were dissociated using papain dissociation kit (Worthington LK003176) for 10 minutes at 30°C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... GFP+ cortices were dissociated using a papain dissociation kit (Worthington) and trituration using glass pipettes (BrainBits LLC) ...
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bioRxiv - Neuroscience 2023Quote: ... and DNAse in 1x EBSS (all from Worthington, kit LK003150). This cell suspension was gently applied on top of a 5 mL solution of 10 mg/mL ovomucoid inhibitor ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... EBs/NMOs were dissociated by the Papain dissociation kit (Worthington). In brief ...
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bioRxiv - Neuroscience 2020Quote: ... Using a commercially available papain tissue dissociation kit (Worthington Biochemical, LK003150), hippocampi are transferred to Earle’s Balanced Salt Solution (EBSS)-based papain solution supplemented with DNase in a 6cm petri dish pre-equilibrated in 5% CO2 36°C incubator and chopped for 30 seconds with Bonn scissors before being returned to incubator for a 10-to 40-minute incubation commiserate with pup age ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... the tissues were transferred in PDS Kit-Inhibitor solution (#LK003182, Worthington). Tissue chunks were triturated ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Neuroscience 2020Quote: The forebrain was dissociated by the papain dissociation kit (LK003176, Worthington) and dissociator (Miltenyi Biotec ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... Isolated tissues underwent dissociation using a papain dissociation kit (#LK003176, Worthington) supplemented with DNase I (250 U/ml ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... Papain was inactivated using an Ovomucoid inhibitor (Worthington Papain Dissociation Kit) and dissociated cells were passed through a 20 μm cell strainer ...
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bioRxiv - Genomics 2023Quote: ... Cells were dissociated using the Papain cell dissociation kit (Worthington, LK003150). Chromium 3’ single-cell RNA-seq kit (10xGenomics ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Microbiology 2024Quote: ... washed once with DPBS and dissociated using Papain Dissociation Kit (Worthington Biochemical Corporation ...