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Citations for Worthington Biochemical :
501 - 550 of 705 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Lung tissue was minced and digested in a RPMI 1640 solution with Type 2 collagenase (0.5 mg/mL) (Worthington Biochemical) and 5 units/mL DNAse (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Ganglia were transferred to dissociation media (divalent cation-free HBSS with 2 mg/mL collagenase Type II (CLS2, Worthington, USA) for 60 min at 37 °C ...
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bioRxiv - Physiology 2022Quote: Digestion of the left kidney was performed by a 20 minutes-incubation in HBSS containing type II collagenase at 0.5 mg/ml (Worthington, USA), followed by a 4 minutes-incubation in trypsin 0.04% at 37°C after removal of the capsule ...
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bioRxiv - Developmental Biology 2021Quote: ... anterior tips of ovaries we transferred to a 1.5 ml tube and were incubated in dissociation solution (0.25 mg/ml Type I Collagenase, 0.4 mg/ml elastase (Worthington Biochemical #LS002290) in Cell Dissociation Buffer (Thermo Fisher Scientific #13151014) ...
Product cited:
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme...Cat# LS002290, 25 mg, $55.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... The remaining cut tissue sections were weighed and incubated with 50 U/mL collagenase type 3 (#CLS-3, CAT#LS004182, Worthington) digestion buffer (at ratio of 8μL / mg tissue ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2021Quote: ... The ventricles were cut into 1-2mm pieces and the cardiac myocytes isolation was achieved by digestion with 0.04% trypsin and type II collagenase (Worthington #LS004176) 0.4mg/ml in KH buffer at 37°C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Bioengineering 2020Quote: ... Sample dissociation was performed in 500 µL of PBS + 25% FBS and 100 Units Collagenase Type IV (#LS004186, Worthington Biochemical). Samples were made piecemeal using scissors ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2022Quote: ... which consisted of HBSS containing Ca2+ and Mg2+ supplemented with 3.4 mg/ml collagenase type 1 (Worthington Biochemical Corporation LS004196) and 100 U/ml hyaluronidase (Sigma-Aldrich H3631) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... The tumor-bearing left lungs were mechanically dissociated using a razor blade and incubated for 30 minutes at 37°C with 3.2mg/mL Collagenase Type 2 (Worthington, 43C14117B), 0.75 mg/ml Elastase (Worthington ...
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bioRxiv - Physiology 2022Quote: ... before perfusion was switched to EGTA-free isolation buffer supplemented with 1 mg/mL collagenase (type II; Worthington Biochemical, USA), 0.06 mg/mL protease XIV ...
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bioRxiv - Physiology 2020Quote: ... minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Immunology 2020Quote: ... Lungs were instilled with either 1 mL of 10% formalin for histology studies or 1 mL of digestion buffer (90%DMEM 10% PBS+Calcium with 180 U/mL Type 2 Collagenase (Worthington) and 15 μg/mL DNase (Sigma ...
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bioRxiv - Physiology 2020Quote: ... muscles were surgically removed and enzymatically dissociated at 37 °C under mild agitation for ~1 h using 1,000 U/mL of collagenase type IV (Worthington Biochemical). Mechanical dissociation was completed using mild trituration in buffer with no collagenase ...
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bioRxiv - Immunology 2020Quote: ... the aortas were then cut into small pieces (1-2 mm) and incubated for 45 minutes at 37 °C in a type I collagenase (6 mg/ml, Worthington) and elastase (2.5 mg/ml ...
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bioRxiv - Genomics 2020Quote: ... After removal of the epidermal sheets, the dermis was finely minced and enzymatically digested (RPMI basal medium, 1000U/mL collagenase type 1 (Worthington), 40µg/mL DNase I (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse hindlimb muscles were dissected and incubated in digestion media (2.5 U/mL Dispase II; Thermo and 0.2% w/v Collagenase Type II; Worthington in DMEM) on a shaking incubator at 37°C for 1.5 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Each 25 μL piece of vitreous was placed in an 8 mm diameter coverslip-bottom chamber with 200 μL of either 2 mg/mL bacterial collagenase type II (Worthington) or phosphate-buffered saline ...
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bioRxiv - Immunology 2022Quote: ... the digested skin was minced and further processed through a 37ºC incubation with 0.2% Collagenase Type 1 (Worthington Biochemical Corporation) in DMEM-F12+ 10% FBS for 1 1/2 hour ...
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bioRxiv - Immunology 2022Quote: ... the digested skin was minced and further processed through a 37ºC incubation with 0.2% Collagenase Type 1 (Worthington Biochemical Corporation) in DMEM-F12+ 10% FBS for 1 1/2 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... limb buds from the E12.5 mouse embryo were isolated and digested with 1 mg/ml type II collagenase (Worthington, LS004176) for 1 h at 37 °C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Genetics 2024Quote: Testicular cell suspension was prepared by incubation of seminiferous tubules in 200 U/ml Collagenase type I (Worthington Biochemical LS004196), 5 μg/ml DNAse I (Roche 10104159001) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2024Quote: ... Spleens and non-draining lymph nodes were digested in 3 mL of 1.6 mg/mL collagenase Type IV (Worthington Biochemical), 0.1 mg/mL DNase I (Thomas Scientific ...
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bioRxiv - Immunology 2023Quote: ... Sciatic and brachial nerves were cut into small fragments and incubated in RPMI 1640 supplemented with 1.6 mg/ml collagenase (type IV Worthington, LS004188) and 200 μg/ml DNase I (Roche ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... The tissue pieces from each sample were weighed and incubated with 50 U/mL collagenase type 3 (#CLS-3, CAT#LS004182, Worthington) in Hibernate-E medium (at ratio of 8μL/mg tissue ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... 300 μL Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC 30-2002) and 150 μL of 10 mg/mL collagenase Type II (Worthington LS004176) were added to each brain and incubated for 5-10 min at 37 °C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2023Quote: ... Hearts were then perfused for 5 - 10 min with 0.125 mM CaCl2 KRH buffer supplemented with 1mg/mL of type II collagenase (Worthington Biochemical). Ventricles were cut into 0.1 mM CaCl2 solution ...
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bioRxiv - Bioengineering 2023Quote: ... the 3D collagen matrices were digested using a 6 mg/ml solution of type IV collagenase (Worthington Biochemical Corporation, USA). The digested collagen matrices were then incubated with Alcian blue 8GX (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... The 3D matrices were then digested using a solution of type IV collagenase (6 mg/mL; Worthington Biochemical Corporation, USA). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... A 2 cm2 patch of skin was minced and incubated in 3 mg/mL collagenase Type IV (Worthington Biochemical Corporation) and 2 mg/mL DNase (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro degradation of lignin composites was determined by submerging lignin composites in the solution of collagenase type II (0.5 U/mL, cat# CLS-2, Worthington Biochemical) with 1 mM CaCl2 in serum-free culture medium ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS and dissociated into single cells using collagenase type 4 enzyme at 200Units/ml (Worthington, Lakewood, NJ, US) for 10min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... followed by enzymatic digestion of the remaining brain in IMDM medium (Cytiva, SH30259.02) supplemented with 1mg/ml Collagenase type IV (Worthington, LS004186) and 33.3U/ml DNase I (Roche ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2023Quote: ... Livers isolated from E16.5 embryos were minced and digested in 6mL of digestion buffer (250U/mL Collagenase type II (Worthington LS004204), 0.6U/mL Dispase (Worthington LS02104) ...
Product cited:
Collagenase, Type 2 (Code: CLS-2), which is filtered through a 0.22 micron membrane and...Cat# LS004204, 5x50 mg, $200.00 Ask -
bioRxiv - Immunology 2023Quote: ... whole lung was minced with a sharp razor blade and digested with collagenase type IV 2 mg/ml (Worthington, LS004189) and deoxyribonuclease II 4 μg/ml (Worthington ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2023Quote: Longitudinal muscle myenteric plexus layers were collected and placed in a digestive KREBS solution buffer with 1.3 mg/mL collagenase type II (Worthington, LS004176) and 0.3 mg/mL BSA (Sigma ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2023Quote: ... The right lobes of the lungs were collected in 5 mL of digestion buffer (90% DMEM and 10% PBS and calcium with type 2 collagenase (180 U/ml) (Worthington) and DNase (15μg/ml ...
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bioRxiv - Immunology 2023Quote: ... Aorta samples were cut into small pieces and then transferred into an enzyme cocktail containing 450 U/mL collagenase type I (Worthington), 250 U/mL collagenase type XI (MilliporeSigma) ...
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bioRxiv - Microbiology 2024Quote: ... lungs were harvested and single-cell suspension was obtained by digesting the tissue in RPMI 1640 containing 10 % FBS and 2 mg/mL of type 2 collagenase (Worthington) and 30 µL/mL of 10 mg/mL DNase (Worthington ...
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bioRxiv - Immunology 2024Quote: ... The cardiac tissues were minced and subjected to repetitive rounds of enzymatic digestion with collagenase type 2 (Worthington Biochemical Corp.) until complete dissociation ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining cut tissue sections were weighed and incubated with 50 U/mL collagenase type 3 digestion buffer (#CLS-3, CAT#LS004180, Worthington) at ratio of 8 μL /mg tissue in a shaking water bath (25°C ...
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bioRxiv - Bioengineering 2024Quote: ... The minced tissues were incubated in digest solution (2.5 U/ml Dispase II, MilliporeSigma and 0.2 w/v % Collagenase Type II, Worthington in DMEM) at 37°C for 90 minutes ...
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bioRxiv - Immunology 2024Quote: Lung tissue was minced and digested in an RPMI 1640 solution with type 2 collagenase (0.5 mg/mL) (Worthington Biochemical) and 5 units/mL DNAse (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary tumor and metastasized lung tissues were digested in 5 mL of enzyme buffer containing 0.2 mg/mL collagenase type-II (Worthington, LS004174), 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... EDL muscles were harvested (by first severing the proximal tendon) and subjected to enzymatic dissociation (collagenase type II, catalog number: LS004177, Worthington) (700 units/mL ...
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bioRxiv - Developmental Biology 2024Quote: ... Kidneys were minced and digested using a freshly prepared enzymatic mix containing Collagenase type 2 (2.5 mg/ml; Worthington, LS004174), DNase I (125 U/ml ...
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bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
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bioRxiv - Neuroscience 2023Quote: ... Human samples were dissociated according to a modified papain-based protocol (Worthington), then filtered through a 40 µM filter before counting and dilution for scRNAseq.
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bioRxiv - Cancer Biology 2021Quote: Tumor-bearing mice were sacrificed and tumors excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...