Collagenase, Type 2
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and salivary primary cell isolation. Supplied as a dialyzed, lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004176 |
| CAS # | 9001.12.1 |
| Pricing | 1 gm, $215.00 |
| Product Code | CLS-2 |
| Activity/Specification | ≥125 units per mg dry weight |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Clostridium histolyticum |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Cell Biology 2020Quote: ... valve leaflets were subjected to 5 mins collagenase (Worthington Biochemical# LS004176) digestion at 37°C and the endothelial layer was gently sheared by sterile swab onto the leaflet ...
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bioRxiv - Neuroscience 2020Quote: ... The collected nerve segments were cut into small pieces with microscissors and incubated in 1 ml collagenase (4mg/ml Worthington Biochemical, LS004176) and dispase (2mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... 12.5 μM CaCl2 and 5 mg/ml Collagenase II (Worthington Biochemical Corp; LS004176) for 1 hour at 32 °C ...
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bioRxiv - Physiology 2021Quote: ... Collagenase (Type 2; LS004176) was purchased from Worthington (USA). All other chemicals were obtained from Sigma-Aldrich.
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bioRxiv - Cell Biology 2021Quote: ... The hearts were subsequently incubated with collagenase type 2 (Worthington-Biochem, Cat# LS004176) supplemented with 1% penicillin-streptomycin (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... and 0.2% collagenase type II (Worthington, Cat#LS004176) at a perfusion rate of 8 mL/minute ...
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bioRxiv - Molecular Biology 2021Quote: ... The ventricles were cut into 1-2mm pieces and the cardiac myocytes isolation was achieved by digestion with 0.04% trypsin and type II collagenase (Worthington #LS004176) 0.4mg/ml in KH buffer at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... The matrix was dissolved with 2 mg/ml collagenase type2 (#LS004176, Worthington Biochemical corporation) and cell numbers were quantified using hemocytometry ...
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bioRxiv - Cancer Biology 2020Quote: ... The matrix was dissolved with 2 mg/ml collagenase type2 (#LS004176, Worthington Biochemical corporation) and cell numbers were determined by hemocytometry ...
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bioRxiv - Cell Biology 2021Quote: ... The heart was enzymatically digested with 0.5 mg/mL Collagenase Type II (Worthington, LS004176), 0.5 mg/mL Collagenase Type IV (Worthington ...