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Citations for Worthington Biochemical :
451 - 500 of 591 citations for 3' Methyl 1 1' biphenyl 4 amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Tumor-transplanted organoids were transferred to a 1.7 mL Eppendorf tube containing 1 mL Papain supplemented with 50 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Physiology 2024Quote: ... Aortas were transferred into the 6-well plate and then incubated with 1 mg/ml collagenase II (Worthington Biochemical, LS004174) and 1 mg/ml soybean trypsin inhibitor (Worthington Biochemical ...
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bioRxiv - Immunology 2024Quote: ... Livers were digested at 37°C for approximately 1 h in DMEM medium supplemented with 500 U/ml Collagenase II (Worthington). Erythrocytes were excluded using red blood cell lysis buffer (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Surgical samples were minced then digested for 1 h at 37°C with 2 mg/mL collagenase I (Worthington, #LS004197) and 200 U/mL deoxyribonuclease (Worthington ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 sutures each from litter 2) were cut into small fragments in HBBS and digested using Collagenase IV (Worthington, USA ...
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bioRxiv - Neuroscience 2021Quote: ... Thermo Fisher, 31966-021 with 3.6 mg/ml glucose, Carl Roth, NH06.3, 3 mg/ml Collagenase type IV, Worthington, LS004186 ...
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bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (3 months old) were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70µm and 40µm cell strainers (Falcon #07-201-431 and #07-201-430) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Bioengineering 2021Quote: ... The solution was replaced with 200 μl trehalose-HEPES-ACSF containing 3 mg/ml ovomucoid inhibitor (OI-BSA, Worthington) and 1 μl DNase I ...
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bioRxiv - Neuroscience 2020Quote: ... and resuspended in 3 ml HA –Ca containing 10% v/v ovomucoid (Worthington, resuspended in 32 ml HA –Ca). Cell debris was removed using discontinuous density gradient containing 3 ml tissue mixture on top of 5 ml ovomucoid solution ...
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bioRxiv - Neuroscience 2021Quote: ... Dissected brains were chilled on ice and minced in digestion media containing with 0.2% collagenase type 3 (LS004182, Worthington) and 3 U/mL dispase (LS02104 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... followed by incubation at 37° C for 25 minutes in 3 mg/mL collagenase type 2 (LS004174, Worthington Biochemical) and 2 mg/mL Dispase II (4942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... and spleen (n = 3; 12-16 PCW) samples were diced and digested with 1.6mg/mL collagenase type IV (Worthington) in RPMI (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: We extracted cells from the central body of freshly dissected menisci from 3-week-old WT and Col5a1+/− mice by 0.2% collagenase (CLS-2, Worthington) digestion for 5 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the tendons were minced to 1mm3 pieces and digested with 3 mg/ml collagenase I (Worthington biochemical, cat# LS004194) in DMEM (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were pelleted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DNaseI (Worthington Papain system ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pelted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DnaseI (Worthington Papain system ...
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bioRxiv - Immunology 2024Quote: ... for RNA extraction or into a digestion buffer containing 1.6 mg/ml collagenase type 4 (Worthington LS004189) and 100 µg/ml DNase I (Roche 11284932001 ...
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bioRxiv - Cancer Biology 2021Quote: Tumor-bearing mice were sacrificed and tumors excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: Single myofibers were isolated from extensor digitorum longus (EDL) muscles by 1 hour digestion in 0.2% collagenase type I (Worthington Biochemical Corporation) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Bioengineering 2020Quote: ... Infection with the H1N1 virus was performed in the presence of 1 µg of TPCK (tolylsulfonyl phenylalanyl chloromethyl ketone)-trypsin/ml (Worthington Biomedical).27 Briefly ...
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bioRxiv - Cell Biology 2021Quote: Epididymal fat pads from mice were minced in Krebs-Ringer phosphate buffer and digested with 1 mg/ml collagenase type I (Worthington Biochemical) at 37°C for 1 h as described in the literature (58) ...
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bioRxiv - Physiology 2021Quote: ... adjusted with NaOH) and then incubated in an enzyme cocktail containing 1 mg/mL papain (Worthington Biochemical Corp., Lakewood, NJ, USA), 1 mg/mL dithiothreitol (DTT ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions were generated from Achilles tendons of MaFIA mice by enzymatic digestion with a solution containing 1 mg/mL collagenase I (Cat. # LS004196, Worthington Biochemical) and 5 mg/mL collagenase IV (Cat ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... Cortices were digested at room temperature for 1 hour using papain solution composed of 20 units/ml papain (Worthington Biochem, LS003126), 1.5 mM CaCl2 (Sigma Aldrich ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Developmental Biology 2020Quote: Two organoids per experimental condition were cut into 1 mm2 pieces and enzymatically digested with pa apain digestion kit (Worthington, LK003163) with the addition of 0.5 mg/ml DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Immunology 2020Quote: ... TIL were harvested by dissection of tumor tissue into small fragments in a 50 ml conical tube followed by digestion at room temperature in a bacterial shaker at 180 rpm for 30 minutes in 1 mg/ml collagenase type IV (Worthington Biochemicals), 100 ug/ml hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: CHO cells stably expressing wild-type SARS-CoV-2 S were resuspended in wash buffer (PBS 1 % BSA, 2 mM EDTA) and treated with 10 µg/mL TPCK-trypsin (Worthington Biochem) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (Worthington Biochemical) at 37°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... The digested samples were washed with PBS and were further digested with 0.05% trypsin-EDTA for 5 minutes and 1 unit/ml neutral protease (dispase) (Worthington Biochemical, LS02109) plus 100 ug/ml Dnase (Roche ...
Product cited:
Animal Free, Partially purified. A lyophilized powder.Cat# LS02109, 1 gm, $171.00 Ask -
bioRxiv - Immunology 2024Quote: ... these tissues were finely minced and then digested at 37℃ in digestion buffer: RPMI 1640 (UCSF Media Production) with 1 mg/mL collagenase IV (Worthington Biochemical) and 0.1 mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Biopsies were mechanically minced with sterile scissors into fragments of less than 1 mm3 and enzymatically digested with 20 mg/mL papain (Worthington #20132), 0.7mg/mL hyaluronidase (Sigma #V900833 ...
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bioRxiv - Genomics 2023Quote: ... Each sample was centrifuged at 1200 rpm x5 mins at 4°C and resuspended in 10 mL warmed digestive media (HBSS + 1% HEPES) with 1,000 U/mL collagenase type IV (Worthington, Cat: LS004188), and rotated for 30 min at 37 °C ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Cortices were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse cortical neurons were plated on following poly lysine-coated glass bottom dishes (500,000 cells) ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Hippocampi were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse hippocampal neurons were plated on following poly lysine-coated dishes for each experiment - glass bottom dishes (500,000 cells ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... the tissues were further transferred into 20 mL of pre-warmed digestion buffer (RPMI medium containing 10% FBS, 1% P/S, 15 mM HEPES, 25 U/mL of collagenase IV (Worthington LS004186), 100 U/ml of DNase I (Sigma D4513) ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2023Quote: Tumor-bearing mice were sacrificed and tumors excised and digested at 37°C for 30 min using a cocktail of 1 mg/ml collagenase type IV (Worthington Biochemicals) and 0.02 mg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... hACs were isolated using 10 ml of a 0.15% (w/v) type II collagenase (300 U mg−1; Worthington Biochemical Corporation) solution per g of tissue (37 °C ...
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bioRxiv - Cancer Biology 2023Quote: The largest tumors from female C3-TAg and PyMT mice were minced and dissociated for up to 120 min at 37°C in a mixture of 1 mg/ml Collagenase (Worthington, LS004188), 20 U/ml DNase (Invitrogen ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2023Quote: Two organoids per experimental condition were washed with Ca2+/Mg2+-free DPBS and cut into 1 mm2 pieces and enzymatically digested with papain digestion kit (Worthington, LK003163) with the addition of DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Homogenate was digested for 15 minutes in enzymatic solution (0.2 µm filter sterilised 4.5 U/mL papain [Worthington Biochemicals], 1× DNAse [Worthington Biochemicals], in PBS) at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... WAT depots from Scd1fl/fl mice were minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Bioengineering 2023Quote: ... constructs were extracted from devices and incubated on an orbital shaker for 1 hour in a solution of 0.15% type II collagenase (Worthington Biochemical Corporation) in DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... hindlimb muscles from mice and hamstring muscles from humans were digested with collagenase II (LS004177, Worthington, 1000 units per 1 ml) for 90 min at 37 °C ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... and hearts were perfused retrogradely for 15-18 min at 37°C with HEPES-buffer containing 100 mg/mL collagenase type 2 (Cat#9001-12-1, Worthington, USA) and digested ...
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bioRxiv - Neuroscience 2024Quote: ... Terminal differentiation of oligodendrocytes was achieved in oligodendrocyte differentiation media by mitogen withdrawal for 7 days (except for T3 and IGF-1) following single-cell dissociation using Papain (20 units/ml) dissociation kit (Worthington Biochemical) and plating on Matrigel (SLS ...
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bioRxiv - Microbiology 2024Quote: ... 1% MEM vitamin solution and freshly added 1.0 μg/ml tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corporation, Lakewood, NJ) incubated at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... minced vessels were incubated for 60 minutes at 37°C with agitation in an enzymatic cocktail consisting of 1 mg/ml collagenase I (Worthington, LS004196), 1 mg/ml Dispase II (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: Spleens were collected into RPMI 1640 containing 2% fetal calf serum (FCS) and dissociated with a scalpel and type 3 collagenase (Worthington) and DNAse I ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 pooled coronal sutures were rinsed in PBS and enzymatically dissociated with a final concentration of 3 mg/mL of Collagenase II (Worthington) and 4 units/mL of Dispase (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated at 37°C for 3 minutes in a solution of HBSS/0.2% glucose with 10 mg/ml trypsin (Worthington Biochemical) and 1 mg/ml Deoxyribonuclease I (Worthington Biochemical) ...