Collagenase, Type 3
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. Suggested for mammary primary cell isolation. A dialyzed, lyophilized powder.
| Supplier | Worthington Biochemical |
|---|---|
| Product # | LS004182 |
| CAS # | 9001.12.1 |
| Pricing | 1 gm, $215.00 |
| Product Code | CLS-3 |
| Activity/Specification | ≥100 units per mg dry weight |
| UNSPSC Code(s)* | 12352204.0 |
| Source | Clostridium histolyticum |
| Storage Conditions | Store at 2-8°C. |
Citations for this product:
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bioRxiv - Molecular Biology 2020Quote: ... The remaining cut tissue sections were weighed and incubated with 50 U/mL collagenase type 3 (#CLS-3, CAT#LS004182, Worthington) digestion buffer (at ratio of 8μL / mg tissue ...
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bioRxiv - Neuroscience 2021Quote: ... Dissected brains were chilled on ice and minced in digestion media containing with 0.2% collagenase type 3 (LS004182, Worthington) and 3 U/mL dispase (LS02104 ...
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bioRxiv - Neuroscience 2022Quote: ... and the cells dissociated for 30 min at 37 °C with 0.2% collagenase type III (Worthington LS004182) in Hibernate-A medium (Thermo A1247501) ...
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bioRxiv - Immunology 2024Quote: Mammary glands were minced and digested with 300 units/ml collagenase type 3 (Worthington Biochemical, LS004182), and 10 μg/ml Dnase I (Worthington Biochemical ...
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bioRxiv - Cancer Biology 2024Quote: ... fresh tumors were dissociated into single-cell suspension by incubating with collagenase III (1 mg/ml, Worthington, LS004182) and DNase I (0.1 mg/ml ...
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bioRxiv - Immunology 2022Quote: ... and 1 mg/mL collagenase III (Worthington, LS004182) for at least 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... or Collagenase-III (for breast tumors) (Cat: LS004182, Worthington), along with Dispase-II (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces from each sample were weighed and incubated with 50 U/mL collagenase type 3 (#CLS-3, CAT#LS004182, Worthington) in Hibernate-E medium (at ratio of 8μL/mg tissue ...
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bioRxiv - Cancer Biology 2023Quote: ... Mammary glands were minced into 1 mm3 size using a tissue cutting blade and digested with 0.5 mg/mL Collagenase type III (Worthington, LS004182) and 50 U/mL hyaluronidase (Worthington ...
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bioRxiv - Cancer Biology 2023Quote: ... organs were dissociated mechanically and enzymatically with collagenase type III (Worthington Biochem, Cat#LS004182) at 37°C to single cell suspension and GFP+ and GFP- cells were collected from each organ (lung ...