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Citations for Worthington Biochemical :
551 - 591 of 591 citations for 3' Methyl 1 1' biphenyl 4 amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... and skin was cut into small pieces and mixed with digestion buffer containing 0.8 mg/ml Collagenase Type 4 (4188; Worthington), 0.02 mg/ml DNAse (DN25-1G ...
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bioRxiv - Neuroscience 2020Quote: ... Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml, Worthington, Lakewood, NJ) and chondroitinase-ABC (1 U/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was collected by centrifuged at 300 g for 5 min and then resuspended in 3 mL Ovomucoid protease inhibitor with FBS (Inhibitor-BSA Vial [LK003182], Worthington, Lakewood, NJ). To remove the debris ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Cell Biology 2024Quote: ... the RPE cells were washed at least 3 times with PBS to remove the dead cells and dissociated using Papain (Worthington Biochemical Corporation) for 30mins-1h at 37 °C following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... Cortices were isolated from postnatal day 0 (P0) CD-1 or cGKII KO mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp. Lakewood, NJ). Mouse cortical neurons were plated on following poly lysine-coated dishes for each experiment - 24 wells (200,000 cells ...
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bioRxiv - Microbiology 2022Quote: Enzymatic digestion of lung tissue was performed by intratracheal instillation via a 20-gauge catheter of 1 mL of 5 mg/mL collagenase I (Worthington Biochemical Corp, Lakewood, NJ) and 0.25 mg/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and the lateral SVZs were dissected in cold DMEM-F12-glutamax 1/50 Glc 45% and subsequently incubated in digestion medium (papain [Worthington], DNase [Worthington], L-cysteine [Sigma-Aldrich]) for 30 min at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Lungs were digested by intratracheal instillation via a 20-gauge catheter of 1 mL of 5 mg/mL collagenase I (Worthington Biochemical Corp, Lakewood, NJ) and 0.25 mg/mL DNase I (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Virus-infected cells were grown in MEM-BSA-TPCK media[20] that was prepared similar to the MEM media described above but supplemented with 1 µg/mL Tosyl phenylalanyl chloromethyl ketone (TPCK) trypsin (Worthington Chemical Corporation, Lakewood, NJ) and with Bovine Albumin Fraction V (7.5% solution ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM Na-pyruvate) and dissociated them by adding an equal volume of 2 x Papain stock solution (Worthington Biochemical Corporation, Lakewood, USA) at 37°C for 20 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 24 h post-transfection the media was supplemented with 1 μg/mL of tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals, Lakewood, NJ, USA). The rgH9N1 viruses were propagated on MDCK cells and harvested 72 hours post-inoculations ...
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bioRxiv - Molecular Biology 2024Quote: ... The fatty and adventitial layers were removed through an 8–10- minute digestion with an enzyme cocktail consisting of 1 mg/ml collagenase type II (Worthington Biochemical Corp, catalog #LS004176), 1 mg/ml soybean trypsin inhibitor (Worthington Biochemical Corp ...
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bioRxiv - Microbiology 2024Quote: ... intramuscular fat of Anguilla japonica was digested with equal volume (v/w) of HBSS containing 1 mg/ml of collagenase type IV (Worthington Biochemical Corporation, NJ, USA) and 5 mM calcium chloride in 37°C for 1 hour ...
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bioRxiv - Plant Biology 2024Quote: ... and incubated at 37 °C for 1 h followed by the addition of 1.5 mg/mL trypsin inhibitor (Worthington Biochemical Corp, Lakewood, NJ USA) to inactivate trypsin ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were incubated for 45 minutes in Hank’s solution containing 30 µL of 50 mg/mL Collagenase I (Worthington, Code #CLS-1, 230U/mg) and 7.5 µL of 50 mg/mL Dispase (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... The dissected hypothalamuses were further divided into 2-4 pieces before cell dissociation for single cell isolation using Papain Dissociation System (cat no. LK003150, Worthington Biochemical Corporation ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Bioengineering 2020Quote: ... acellular PCL and GA or GP crosslinked dMEP scaffolds (n = 4/group) were digested in 2 mg/mL collagenase type 2 solution (Worthington) for 24 h at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and enzymatically digested at 37°C for 80 min in dispase type II (4.7 mg/ml) plus collagenase type II (4 mg/ml) (Worthington Biochemical), as described previously [62] ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS and dissociated into single cells using collagenase type 4 enzyme at 200Units/ml (Worthington, Lakewood, NJ, US) for 10min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA was then digested for 2 hr at 37 °C in 1/10 volume of 1 M ammonium bicarbonate with snake venom phosphodiesterase (1.2×10−4 U/μg RNA) and bacterial alkaline phosphatase (0.1U/ μg RNA) (Worthington Biochemical corporation). Analysis was performed on a TSQ Quantiva Triple Quadrupole (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... the digestion was stopped by a solution of trypsin inhibitors (Leibovitz’s L15 medium, 10% 1 mg/ml trypsin inhibitor [Worthington], and 2% 10 mg/ml DNAse) and cells were washed with L15 medium ...
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bioRxiv - Genetics 2021Quote: Left hind legs of P7 mice were isolated from one WT and one Col12a1-/- mouse and digested in 4 mg/mL collagenase II (Worthington, USA) in DMEM/F-12 (supplied with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor samples were transferred into a 5mL Eppendorf tube containing 4.5mL cold enzymatic dissociation mix (100µg/mL Collagenase Type 4 (Worthington Biochemical Corporation #LS004186) and 10 µg/mL DNAse I (StemCell Technologies #07900 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Biochemistry 2024Quote: ... epidydimal fat from 4-month-old male DIO C57BL/6J mice was dissected out and dissociated using 2 mg/ml Collagenase B (Worthington, CLSAFB) and 1 mg/ml soybean trypsin inhibitor (Worthington ...
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bioRxiv - Genetics 2023Quote: ... and perfused with a type 2 collagenase digestion buffer (2.4 mg/ml concentration) at a flow rate of 4 ml/min (Worthington Cat# LS004176). The large blood vessels and the atria were excised from the ventricles and minced in a stop buffer containing 10% calf serum ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Synthetic Biology 2024Quote: ... and both fragments were separately digested in a petri dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) for 12 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Each tissue was cut and minced with scissors and then placed in 15 ml conical tubes containing digestion buffer (2 ml DPBS and Collagenase II at 3 mg/ml; Worthington Biochemical, Lakewood, NJ, USA) for 40 min of incubation at 37°C with gentle shaking at 100 rpm ...
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bioRxiv - Cancer Biology 2024Quote: The ovarian cancer tissues were incubated in DMEM high glucose culture medium supplemented with 3 mg/ml collagenase D (Worthington-biochem, Lakewood, NJ, USA) at 37°C for 45 min ...
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bioRxiv - Neuroscience 2020Quote: Brain samples were minced with scissors and then incubated at 37 °C on a roller for 30 min in digest buffer containing 1.25 mg/mL Collagenase Type 4 (Worthington, Lakewood, NJ, USA) and 100 U/mL DNAseI (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: 30 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 60 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Bioengineering 2024Quote: ... The DRGs from 4 pups were digested for 30 min at 37°C using 13.33 U/ml papain (Worthington, cat. No. LS003126). The DRGs were then centrifuged for 1 min at 500g ...
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bioRxiv - Immunology 2023Quote: ... and then digested for 30 minutes under gentle agitation at 37°C with 7 mL of 4 mg/mL of type III collagenase (Worthington Biochemical, Lakewood, NJ) and 0.05 mg/mL of DNase I (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... The SNc regions were dissected out from each slice and incubated for 20–30 min at 36–37℃ in oxygenated high-glucose HEPES-buffered saline containing 4–8 U/ml papain (Worthington Biochemical Corp., USA, LK0003176). After enzymatic digestion ...
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bioRxiv - Neuroscience 2024Quote: ... ∼20 hCOs were chopped into small pieces using a #10 blade and treated with 30 U/ml Papain (Worthington Biochemical, CAS: 9001-73-4) for 60-90 minutes at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... These chunks were immersed in 5 ml of pre-warmed enzyme solution (2 mg/mL STEMzyme I, 4 µg/mL DNAse I obtained from Worthington Biochemical #LS004107 and #LS002139) in Hanks’ Balanced Salt Solution (HBSS ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004107, 5x50 mg, $495.00 Ask