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Citations for Qiagen :
951 - 980 of 980 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... and from stool samples from 10 volunteers was extracted using QIAamp® PowerFecal® DNA Kit (QIAGEN, catalog no. 12830–50; Hilden, Germany). The genomic DNA was stored at m –20°C until PCR amplification and amplicon sequencing.
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bioRxiv - Microbiology 2021Quote: ... 1 μL of 20 mM of each primer (KAN-2 FP1 or KAN-2 RP1 complementary to the Tn5 sequence with the bubble primer 224) and 10 μL of the 10X Qiagen Multiplex PCR Master Mix Kit (Qiagen, Valencia, CA, USA) in a final volume of 100 μL ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA from 4-8 microdissected 10 μm FFPE sections were extracted using the QIAamp DNA FFPE Tissue kit (Qiagen, cat. no. 56404) according to manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... About 30-40 flowers were pooled together from 8-10 different plants for each biological repeat and RNA was isolated using RNAeasy plant mini kit (Qiagen, Germantown, MD, USA). RNA was converted to cDNA using PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Biophysics 2022Quote: ... 100 µl of the protein extract were then supplemented with 400 µl of homogenization buffer containing 10 mM imidazole and added to 50 µl Ni2+-NTA agarose beads (Qiagen GmbH, Hilden, Germany) preconditioned with washing buffer (0.1 M sodium phosphate buffer (pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from between < 10 and 300 mg dry weight of gut content using the MoBio Powersoil HTP 96 Soil DNA Isolation Kit (Qiagen, Valencia, CA, USA) according to the DNA extraction protocol (v ...
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bioRxiv - Cancer Biology 2019Quote: FFPE samples were sliced into 10-μm sections and gDNA was extracted using the QIAamp DNA FFPE Tissue Kit (Qiagen, Sussex, United Kingdom). DNA concentration was determined using the Quant-iT™ PicoGreenR dsDNA Assay Kit (Thermo Fisher Scientific UK Ltd. ...
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Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... The supernatant was transferred to clean 1.5mL MCF tube and to which 350 μL ethanol at 4’C was added before vortexing for 10 sec and transferring the supernatant to a QIAamp Mini spin column (Qiagen Germantown, MD U.S.A.). The sample was process as recommended in the QIAamp DNA Mini Handbook and eluted with 10 mM Tris-Cl ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from the gill samples collected from both Lake Winnipeg walleye (n = 10) and Delta Marsh walleye (n = 21) using the RNeasy Plus Mini Kit (Qiagen, Toronto, ON, CA) following manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: RNA was extracted from mouse islets (∼300 islets per sample, pooled from ∼10 mice of the same genotype) using RNAeasy Micro Kit (Qiagen, Valencia CA, USA) as per manufactures instructions ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from between < 10 and 300 mg dry weight of gut content using the MoBio Powersoil HTP 96 Soil DNA Isolation Kit (Qiagen, Valencia, CA, USA) according to the DNA extraction protocol (v ...
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bioRxiv - Microbiology 2022Quote: ... exposed to 0.4 nM and 4 nM of β-estradiol for 10 days) Vagina Chips using QIAzol lysis reagent (Qiagen, cat. no. 79306). Complimentary DNA was synthesized using a SuperScript VILO MasterMix (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... and 1:10 dilution of cDNA was used to quantify genes using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, USA). ADP-Ribosylation Factor 1 (AB050957.1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was extracted in a pooled reaction (up to 10 wasps from one brood per reaction) using the Qiagen DNAeasy blood & tissue kit (QIAGEN Inc, Hilden, Germany). After extraction DNA was amplified in a multiplex PCR reaction using fluorescently labelled Lysi07 primers ...
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bioRxiv - Neuroscience 2023Quote: ... A sample of the resulting homogenate (10-20 mg) was used to assess total cellular RNA using the RNeasy® Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Biopsies no larger than 5 x 10 x 10mm were washed with ice-cold PBS three times and placed in RNAlater™ (Qiagen, Germantown, MD). After a minimum incubation of 24 hours in RNAlater at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Lysates were incubated for 1 hr at 37°C after adding 10 µl of RNAse A (20 mg/ml) and proteins were precipitated with 200 µl of Protein Precipitation Solution (Qiagen Gentra Puregen Cell kit). After centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... Enzyme was inactivated at 95 ° C for 10 min and 700 μl Trizol was added followed immediately by RNA extraction using the miRNeasy kit (Qiagen Inc., Valencia, CA, USA).
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bioRxiv - Biochemistry 2019Quote: ... Reactions were quenched at different time points (2, 5, 7, 10 and 20 minutes) by the addition of 250 μL of PB buffer (Qiagen QIAquick PCR Purification Kit) supplemented with 10 mM of EGTA ...
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bioRxiv - Microbiology 2019Quote: DENV viral RNA extraction was performed from acute serum samples (Baseline, 1-10, and 15 dpi) using QIAamp Viral RNA mini kit (Qiagen Inc, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a 10 mL culture using the QIAGEN® Genomic DNA Buffer Set and Genomic-tips™ 100/G set (midi-prep) (QIAGEN, Hilden, Germany). This procedure adhered to the Sample Preparation and Lysis Protocol for Bacteria as outlined in the QIAGEN® Genomic DNA Handbook ...
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bioRxiv - Pathology 2024Quote: ... 4 sections 10 µm thick were placed in a chilled Eppendorf tube and the RNA was extracted using a protocol from Qiagen (Rneasy FFPE Kit 73504), following extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 µl buffer RLT plus with 2-Mercaptoethanol (βME) at 10 µl βME/ml of RLT+ was added before placing the tube into a TissueLyser LT (QIAGEN Germantown, MD, USA; Cat#69980) set at 50 oscillations/second for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... and aged (n=10) RVLM-containing tissue punches was reverse transcribed to cDNA using an RT2 preamp cDNA synthesis kit (Qiagen, Inc., First strand cDNA synthesis) following manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... RNA and protein were digested in 40 mM Tris-HCl pH 6 and 10 mM EDTA by adding first RNAse A (Qiagen; #19101, 1.5 hours, 37°C), followed by Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... DNA extractions using higher sediment volumes (up to 10 grams) with Qiagen DNeasy PowerMax Soil led also to low DNA yields (similar ranges as those reported for Qiagen DNAeasy Powersoil Pro kit tests). Because higher volumes of sediment also increase the concentrations of inhibitory compounds found in Guaymas subsurface sediments ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA pellets were washed 3X with 70% ethanol and hydrated in pre-warmed (37°C) Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5. Cat. 19086) and incubated on HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... The total DNAs of 10 samples were extracted from wheat and barley plants using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany, Cat No./ID: 69104) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... leaf-disc punches were collected from plants and ground in 250 µL of 10 mM MgCl2 using the TissueLyser II (QIAGEN; 2 cycles of 30 seconds at 25 Hz) and 3-mm zirconium oxide beads (Glen Mills Inc.) ...