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Citations for Qiagen :
501 - 550 of 980 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The qPCR reaction mixture (10 μL) comprised 1× Rotor-Gene SYBR green PCR mix (Qiagen), 500 nM of each primer ...
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bioRxiv - Microbiology 2019Quote: ... 9 mL 1M Tris HCl pH 7.5, 9 mL 0.5M EDTA pH 8.0, 11.25 mL 10% SDS, 22.5 mL Qiagen lysis reagent ...
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bioRxiv - Biochemistry 2019Quote: ... 10 ng cDNA was used for qPCR with Rotorgene 6000 system (Corbett Research, now Qiagen) with the QuantiNova SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2021Quote: ... for 10 min at a frequency of 30 beats per second by TissueLyser II (Qiagen). The recorded fluorescence intensities were normalized to CFU/cm2 and reported in RFU/CFU.
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bioRxiv - Systems Biology 2020Quote: ... The entire oligo pool (lyophilized, 10 pmol) was dissolved in 100 μ! Elution buffer (Qiagen) and shaken at room temperature (RT ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted from proliferating leaves at 10-12 DAS using QIAzol lysis reagent (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... A 10% homogenate was prepared in PBS using a Tissue Lyser II (Qiagen, Manchester, UK) and stainless steel beads ...
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bioRxiv - Neuroscience 2021Quote: ... n=10) was homogenized using the PowerGen 125 handheld homogenizer in QIAzol Lysis Reagent (Qiagen) and extracted using the RNeasy Plus Universal Mini Kit (Qiagen) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysate (20 mL) was incubated with 10 mL of Ni-NTA agarose (QIAGEN, 30230) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA (at least from 2.5* 10^6 cells) was purified onto RNeasy columns (Qiagen) and treated on-column with DNase (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... The reagents used were from the Type-it Microsatellite PCR Kit from QIAGEN in a total volume of 10 μl: 2.5 μl master mix (Type-it Kit, QIAGEN), 0.2 μl Q (Type-it Kit ...
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bioRxiv - Physiology 2021Quote: ... 1,000 H2BGFP-positive cones per retina were sorted into 10 μL of Buffer TCL (Qiagen) containing 1% β-mercaptoethanol and immediately frozen in −80 °C ...
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bioRxiv - Immunology 2021Quote: ... prepared with lysis buffer consisting in 10 μl of TCL buffer (Qiagen. Cat. No. 1070498) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Genetics 2021Quote: ... by mixing 1 μl of RT product with 10 μl of SYBR qPCR Mastermix (Qiagen) containing the appropriate primers (345 nM of forward primer and 345 nM of reverse primer) ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from 10 female guts using RNeasy Plus Mini Kit (74134; Qiagen), and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... the mouse spinal cord sections were treated with 10 μg/mL proteinase K (QIAGEN, 19131) at 37°C for 5 min and then post-fixed in 4% PFA at RT for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... and Br+10) of tomato plants was isolated using RNeasy Plant Mini Kit (Qiagen, Germany) by following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... corpus (segment 6-7) and cauda (segment 8-10) samples using QIAzol Lysis Reagent (Qiagen) following the manufactureŕs recommendation using a bead-based tissue homogenizer (Retsch ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.2) and dialyzed against the same buffer overnight (10 kDa membrane, ThermoScientific or Qiagen) at room temperature ...
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bioRxiv - Genetics 2024Quote: ... The master mix was composed of 10 µL 2x QuantiTect Multiplex PCR NoROX reagent (Qiagen), 0.5 µM of each primer and 2 µL DNA template ...
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bioRxiv - Genomics 2023Quote: ... and eluted from the MinElute columns with a 10 μL volume of Elution Buffer (Qiagen). Accessible DNA libraries were amplified by PCR using NEBNext High-Fidelity PCR Master Mix (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... which is optimized for fragments up to 1.5 kb (Qiagen Multiplex kit handbook 10/2010). Thus ...
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bioRxiv - Microbiology 2023Quote: ... Samples were homogenized at for 10 min at 30 Hz using a TissueLyser II (QIAGEN). DNA extraction continued per manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The last group was subjected to RNase A treatment (10 μl = 100 μg; Qiagen, 1007885) in PBS at 37°C for 40 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using a 10:1 ratio of binding buffer (a modified version of Qiagen’s PB buffer) to digestive mixture ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM imidazole) and was added to 8 mL Ni-NTA resin (Qiagen, Hilden, Germany) preequilibrated with lysis buffer and rocked for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... washed with 450 µL of EB buffer (10 mM Tris-Cl, pH 8.5; Qiagen, Germany) and centrifuged again at 15.294 × g for 10 minutes to a final volume of 120 µL ...
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bioRxiv - Microbiology 2024Quote: ... Samples were homogenized at for 10 min at 30 Hz using a TissueLyser II (QIAGEN). DNA extraction continued per manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μL of 10 μM double-stranded mature miRNA mimic (Dharmacon) or 20 μM LNA (Qiagen) was mixed with 3 μL lipofectamine RNAiMAX in 100 μL Opti-MEM reduced serum media and added to cells following a 15-minute incubation ...
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bioRxiv - Genomics 2020Quote: ... We subjected samples to a two day 0.5 EDTA and proteinase K (10 mg/mL; Qiagen) digestion ...
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bioRxiv - Microbiology 2019Quote: ... as per the manufacturer and a final elution step of 2×10 μL EB buffer (Qiagen).
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bioRxiv - Microbiology 2019Quote: Whole blood (10 μL) was collected in an RNase free Eppendorf tube containing lysis buffer (QIAGEN) and stored at −80°C until use ...
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bioRxiv - Developmental Biology 2020Quote: ... and resuspended in 10 mM Tris pH8 (500 μL) with 5 μL RNase A (Qiagen 19101) for 2 hours after which samples were treated with 5 μL Proteinase K (Qiagen 19131 ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (10 to 20 ng) was retrotranscribed by using the QuantiTect Reverse Transcription Kit (Qiagen). Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2020Quote: ... then spun at 14000K RPM for 10 minutes at 4°C to pellet debris (Qiagen, 37901). Protein concentration in the supernatant was quantified using BCA assay (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 10 µL of the PCR products were sequenced by pyrosequencing on the PSQ96 HS system (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... for 10 minutes followed by total RNA isolation using the RNeasy Mini kit (Qiagen, Germantown, MD) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... crystals were flash frozen (100 °K) in the above reservoir solution supplemented with 10% glycerol (QIAGEN). The diffraction data set for complexes of Brd4(BD1 ...
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bioRxiv - Cell Biology 2022Quote: HUVECs were transfected at 60% confluency with 10 nmol/L locked nucleic acid (LNA) GapmeRs (Qiagen) or small interfering RNAs (siRNAs ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The samples were vortexed for 10 seconds and loaded onto a genomic DNA column (Qiagen, 10262). Column purification was performed following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... DNA was finally purified using 10 ul of silica beads (QIAEX II Gel Extraction Kit, Qiagen) and eluted in 30 ul elution buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Mary Goldring) and 10 ng of pRL Renilla luciferase plasmid using the Effectene transfection reagent (Qiagen). Cells were transfected for 8 hrs and then cultured for 24 hrs ...
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bioRxiv - Plant Biology 2021Quote: Total RNAs were extracted from 10-day-old seedlings using the RNeasy plant mini kit (Qiagen). Yield and RNA integrity were detected by using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Genomics 2022Quote: ... 10 mL chilled Qiagen Buffer C1 (from the Qiagen Genomic DNA Buffer Set, Qiagen no. 19060) and 30 mL chilled ...
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bioRxiv - Microbiology 2022Quote: ... Cells were harvested by centrifugation for 10 min at 4,000 RPM and resuspended in RNAprotect (Qiagen). RNA was isolated using the RNeasy mini kit (Qiagen) ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA (10 μg) extracted from E19 placenta and liver samples using RNeasy midi kits (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... fabarum in 10 sub-samples (50-100 wasps each) using the QIAmp DNA mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was extracted from 10 ml overnight culture using QIAprep® Spin Miniprep Kit (QIAGEN) with the following adaptations ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... and total RNA was extracted from 10 heads per sample using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL for OD600 ~0.4 for 10% CO2) and stabilized by RNA Protect Bacteria Kit (Qiagen). Next ...