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Citations for Qiagen :
751 - 800 of 980 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: RNA from infected cell lysates was harvested by incubating infected Vero E6 cells for 10 minutes with RLT buffer (Qiagen) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 % (v/v) glycerol) for 30 s at a frequency of 15 s-1 using a TissueLyser II (QIAGEN). As the tissue homogenates were to be used for both native and SDS PAGE ...
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bioRxiv - Cancer Biology 2022Quote: DNA was extracted from 10 micro-meter slices using the Qiagen GeneRead DNA FFPE extraction kit (Qiagen, Catalogue no. 180134) which includes a Uracil N Glycosylase enzyme treatment to reduce FFPE artefacts ...
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bioRxiv - Biochemistry 2022Quote: ... were purified with 25 μL of Mag-bind Total Pure NGS beads and normalised in the same step by taking 10 μL of each reaction after resuspension (Qiagen Elution buffer ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed in TE buffer (pH=8) supplemented with 1 mg/ml of lysozyme and 10 µl of proteinase K (Qiagen) for 10 minutes at room temperature with a shaking of 500 rpm ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from approximately 10 g of sediments for each depth using PowerMax Soil DNA Isolation Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... We extracted total RNA from 10 leaves that were shorter than 500 µm and from 4–6 mature leaves using the RNeasy Micro Kit (Qiagen) and the RNeasy Plant Mini Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was extracted from pooled (n=10) embryonic zebrafish and pooled (n=3) adult (> 1 year) heart samples by QIAGEN RNEasy Lipid Tissue Extraction kit according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the manufacturer’s directions were followed with the exception of adding 500ul of 10% SDS (w/w) to the homogenate at 60°C for 20 minutes before the addition of P2 buffer (Qiagen). This allowed the brain tissue to better solubilize with the Qiagen buffers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 x g for 10 min) and infected cells using a QIAamp Viral RNA MiniKit and RNeasy Plus mini kit (Qiagen), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... 10∼12 ml cell culture medium was subjected to extracellular RNA isolation using the exoRNeasy Maxi kit (Qiagen, Cat# 77164) with minor modifications ...
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bioRxiv - Microbiology 2022Quote: ... with heat inactivation at 75°C for 10 minutes and DNA was further removed using a RNeasy Mini kit (Qiagen). RNA was harvested from three independent cultures as biological replicates per experiment ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from cryopreserved PBMCs at 7 different time-points (10, 33, 56, 94, 120, 185, and 198 wpi) using the AllPrep DNA/RNA mini kit (Qiagen) as per the manufacturer’s specifications ...
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bioRxiv - Microbiology 2022Quote: For RNAseq the pellets were resuspended in 150 µL 10 mM Tris-HCl pH 8 and mixed with 700 µL of ice cold RLT+BME (RLT buffer (Qiagen) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells were eliminated by incubation for 10 min on ice with 5 mL of erythrocyte lysis buffer (Qiagen). Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: ... as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000, Qiagen) for one hour ...
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bioRxiv - Molecular Biology 2019Quote: Individual single Giardia cysts or pools of 10 cysts isolated by FACS were subjected to MDA with the REPLI-g single cell kit (Qiagen). MDA was performed according to the manual ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 1×10^7 PBMCs using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Hilden, Germany). Following nucleic acid isolation ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen right hemibrain was crunched in liquid nitrogen and 10-20mg of pulverized mouse brain was used for RNA extraction with RNeasy Plus Mini Kit (Qiagen). RNA concentration and quality were determined using Agilent RNA 6000 Nano Kit according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Glass beads (3mm) and 1 mL of sterile water were added for homogenization at 30Hz for 10 minutes (TissueLyser II, Qiagen). Homogenates were diluted with PBS and inoculated onto SAB plates and incubated for 3 days at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... samples were weighed and homogenized in DMEM containing 10% FBS and 1% antibiotics using 5 mm stainless steel beads (Qiagen) and the TissueLyser II (Qiagen) ...
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bioRxiv - Bioengineering 2021Quote: ... and 10 mM imidazole was added to the supernatant then loaded to a gravity column packed with Ni-NTA resins (Qiagen) pre-equilibrated with 20 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Cells were then centrifuged at 300 x g for 10 minutes and vigorously resuspended in 350µL RLT Buffer (Qiagen, Germany) by vortexing for 30 seconds ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from ≈10 mg of MCT rat lung tissues using the RNeasy Fibrous Tissue Mini Kit (74704, Qiagen) following to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted using TRIzol according to the manufacturer’s instructions and contaminating DNA was removed by DNase treatment for 10 mins and column purification (Qiagen RNAeasy). cDNA was prepared from 1µg total RNA using qScript reverse transcription kit (Quantabio ...
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bioRxiv - Immunology 2019Quote: ... 10 μl of the gene expression assay (RT2 SYBR Green ROX qPCR Master Mix) (cat. # 330520, Qiagen, Valencia, CA, USA) and 1 μl of the relevant mouse RT2 qPCR Primer Assay ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μm thick CCO/SDH-stained sections and DNA was extracted using the QIAamp DNA Micro Kit (Qiagen, Cat # 56304), using 2 μL of carrier RNA to enhance DNA yield ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µL of the mixture was then incubated for 45-60 minutes at room temperature or 4°C with 10 µL NiNTA resin (Qiagen) prewashed with His-SUMO Lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified extract was supplemented with 10 mM imidazole and rotated for 2 hours at 4 °C with 1 ml of Ni-NTA beads (Qiagen). Bound protein was eluted with 10 CV of buffer L / 200 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: The cells of each positive clone were collected by centrifugation (400 × g for 10 min at room temperature) and the RNA extracted with the commercial kit RNeasy Mini (Qiagen), following the protocol established by the manufacturer ...
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bioRxiv - Biochemistry 2021Quote: ... The Bacmid DNA pellet was then dried for 1 minute and re-suspended in 50 μL Buffer EB (10 mM Tris-Cl, pH 8.5; All buffers from QIAprep Spin Miniprep Kit, Qiagen #27104). Purified bacmid was then transfected into Sf9 cells ...
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bioRxiv - Genomics 2021Quote: We collected high molecular weight genomic DNA from whole blood or tissue from 36 coyotes sampled from Louisiana and 10 red wolves from North Carolina using the DNeasy Blood and Tissue Kit (Qiagen) and followed the manufacturer’s protocol for mammals ...
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bioRxiv - Plant Biology 2021Quote: ... genomic DNA (gDNA) was extracted from 10-day-old 2C and 4C rice seedlings with the DNeasy plant mini kit (Qiagen) per manufacturer’s introduction ...
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bioRxiv - Plant Biology 2021Quote: Total RNAs were extracted from 10-day-old 2C and 4C rice seedlings using the RNeasy plant mini kit (Qiagen). cDNA library construction and sequencing were carried out by Beijing Genomics Institute (BGI ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... cells were either left untreated or treated with 1000 IU/ml recombinant IFN-α treatment and incubated for 6 h or transfected overnight with polyI:C (10 μg/ml) using Polyfect (Qiagen). Luciferase assays were carried out ...
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bioRxiv - Genomics 2020Quote: ... 25 nuclei were sorted into each well of 96-well plates (8-10 plates per experiment) containing 12 µl of nuclear lysis buffer (11 µl of EB buffer (Qiagen) supplemented with 0.5 µl of 100X BSA and 0.5 µl of 1% SDS) ...
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bioRxiv - Genomics 2019Quote: Total RNA was isolated from Arabidopsis seedlings grown for 10 days and exposed to DMSO or splicing inhibitors for 6 or 24 hours with RNeasy Plant Mini Kit (Qiagen) according to manufacturers’ instructions ...
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bioRxiv - Immunology 2021Quote: THP-1 human monocytes or mature primary human peripheral blood derived macrophages were transfected with control (D-001810-10-05, Dharmacon) or SP140 (L-016508-00-0005, Dharmacon) SMARTpool siRNA (100nM) using HiPerfect reagent (Qiagen) or RNAiMAX (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... ML336 resistant mutation emerging in nsP2 at passage 10 was confirmed by purification and reverse transcription of viral RNA from cell supernatants using RNeasy Mini Kit (Qiagen) and SuperScript IV First-Strand Synthesis kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Cells were rinsed at rested at 37°C for a minimum of 1h before undergoing red-blood-cell lysis by 5-10 ml RBC lysis solution (Qiagen) for 20 min at room temperature ...
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bioRxiv - Genetics 2019Quote: ... for the PCR analysis of potential recombination events genomic DNA from 1×10^6 cells was extracted using DNeasy Blood & Tissue Kits (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Cells were pelleted by centrifugation after isolation (500 g for 10 min) and subsequently lysed for RNA isolation using RNeasy Micro Kit (Qiagen). For some assays ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was supplemented with 10 mM imidazole and His-tagged proteins were purified by affinity chromatography on NiNTA column (Qiagen) at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was harvested following an 8 hour DMSO or 10 nM E2 induction with buffer RLT plus (Qiagen, 1053393) supplemented with 1% beta-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was then air dried for 1 minute and re-suspended in 50 μL Buffer EB (10 mM Tris-Cl, pH 8.5; All buffers from QIAprep Spin Miniprep Kit, Qiagen #27104). Purified bacmid was then transfected into Sf9 cells ...
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bioRxiv - Genomics 2022Quote: ... were annealed (10 μL each 100 μM) to 10 μL 5′-[Phos]-CTGTCTCTTATACACATCT-3’ oligonucleotide (100 μM) within 80 μL EB buffer (Qiagen), incubating 2 min at 95°C and cooled to room temperature (0.1°C/sec) ...
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bioRxiv - Plant Biology 2022Quote: ... were added to each sample and samples were homogenized for 10 min in a bead mill (25 Hz, Retsch Qiagen). Samples were centrifuged for 15 min at 14 000 rpm at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 1 mg/mL of lysozyme and supplemented with 10 μL of Proteinase K (QIAGEN, cat. no. 19131). The mixture was incubated on a shaker at room temperature for 30 min with intermittent vortexing ...
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bioRxiv - Microbiology 2022Quote: ... including either whole fecal pellets or 200-300 uL of contents diluted 1:10 (w/v) in sterile PBS using the MagAttract PowerMicrobiome Kit (Qiagen), and prepped DNA libraries as previously described (36) ...