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Citations for Qiagen :
851 - 900 of 980 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted and 5 μg reverse transcribed from paired isolated Jz and Lz placental tissues (n = 8-10 per genotype/sex, across 11 litters) using the RNeasy Plus Mini Kit (Qiagen, DE) and the High-Capacity cDNA Reverse Transcription Kit minus RT inhibitor (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Multiplex amplification was carried out in 10 μl reaction volumes containing 5 μl of QIAGEN Multiplex PCR Buffer Mix (QIAGEN Inc.), 0.2 μM labeled forward primer (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was extracted from 10 samples (5♀♀, 5♂♂) using RNeasy Micro kits (QIAGEN K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 µl of diluted template cDNA (1:3, nuclease-free water) per real-time PCR reaction (10 µl – SYBR Green PCR kit, Qiagen, 204143) was used to assay specific transcript abundance (CFX96 Real-Time System ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (gDNA) was extracted from snap-frozen tissues (∼10–20 mg) using DNeasy Blood and Tissue Kit (Qiagen, Cat# 69504) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was prepared from HT29 cells treated with 10 mM BG for 48 h utilizing RNeasy Mini kit (Qiagen, Germany), and was subjected to microarray and RNA sequencing analyses as described previously (Sasaki et al. ...
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bioRxiv - Immunology 2020Quote: Naso- and oropharyngeal swab samples were centrifuged at 17,000xg for 10 mins at 4°C to obtain cell pellets which were washed once using PBS and lysed by bead-vortexing for 10 minutes in cell lysis buffer (RLT, Qiagen, Germany). Proteins (as well as DNA and RNA ...
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bioRxiv - Immunology 2020Quote: Airway samples were centrifuged at 17,000xg for 10 mins at 4°C to obtain cell pellets which were washed once using PBS and lysed by bead-vortexing for 10 minutes in cell lysis buffer (RLT, Qiagen, Germany). Proteins (as well as DNA and RNA ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with selection plasmid pCoBlast (1:10) and the mixture was used for cells transfection by Effectene Transfection Reagent (QIAGEN, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... total RNA from the fibroblasts cultured with 10 μM Dwn1 was extracted every 6 hours from hours 24 to 48 (RNeasy® Plus Mini kit, Qiagen), followed by cDNA synthesis (SuperScript® VILO™ cDNA Synthesis Kit ...
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bioRxiv - Microbiology 2020Quote: ... Each 10 μL uPCR reaction contained 2 μL of DNA template with 1x QuantiTect Multiplex PCR No ROX mastermix (Qiagen™), 0.4 μM each primer ...
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bioRxiv - Neuroscience 2020Quote: DNA and RNA from 8-10 mice was isolated and purified with AllPrep-DNA/RNA/miRNA-universal kit (Qiagen, Montreal, QC) and concentrations were determined by fluorometry on the Qubit system (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Cells debris was pelleted by centrifugation at 23,000 x g for 45 minutes and the supernatant loaded onto a 10 ml Ni2+-NTA column (Qiagen, Hilden, Germany). The column was washed with 600 ml buffer A and protein batch eluted with 100 ml buffer B (buffer B = buffer A supplemented with 290 mM imidazole).
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bioRxiv - Neuroscience 2020Quote: ... The high-quality reads were de novo assembled using the short reads assembly program Trinity version 2.3.2ref56 as well as a CLC Genomics Workbench version 10 (Qiagen, Carlsbad, CA, USA). The Trinity assembly yielded a total of 74,151 predicted ‘genes’ with their isoforms totaling 171,567 predicted ‘transcripts’ (i.e. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 10-20 mg of silica-dried stem or leaf tissue was ground with a Qiagen TissueLyser II (Qiagen, Melbourne, Vic., Australia). DNA was extracted using the DNeasy 96 Plant Kit or DNeasyPlant Mini Kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... 65-79*SE or 65-79*PE (100 μg/mL) with or without IFN-γ (10 ng/mL) using the RNeasy mini kit (Qiagen) according to the vendor instructions ...
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bioRxiv - Biochemistry 2019Quote: Total RNA extraction was performed on the cell pellet from 10 ml of algal culture using the RNeasy® Plant Mini Kit (QIAGEN). DNase treatment was carried out using TURBO DNA-free™ kit (Ambion) ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Immunology 2021Quote: At least 10,000 sorted cells or 10 μL of whole blood sample were collected for genomic DNA extraction using the DNeasy Blood & Tissue Kit (Qiagen, #69504). 3 μL of genomic DNA was used as template to amplify the sgRNA target region ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was extracted from 10 samples (5♀♀, 5♂♂) using RNeasy Micro kits (QIAGEN K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The sample was then eluted in buffer AE (10 mM Tris-Cl; 0.5 mM EDTA; pH 9.0; Qiagen Inc., Courtaboeuf, France). DNA purity was assessed with a NanoDrop spectrophotometer based on the 260/280 and 260/230 O.D ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN, Manchester, UK) in the absence of carrier RNA ...
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bioRxiv - Microbiology 2021Quote: ... 1.5mL of the culture was pelleted (13500 rpm for 10 min) and used for the extraction of DNA with DNeasy Blood and Tissue Kit (Qiagen, Germany). For eluting nucleic acids ...
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bioRxiv - Genomics 2020Quote: BAC clones were extracted from each of these primary pools using either the Qiagen Large-construct Kit (10) (12462, Qiagen, Germany) or Omega BAC/PAC DNA Maxi Kit (D2154-02 ...
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bioRxiv - Microbiology 2022Quote: ... Soils were sampled at 10 cm depth from the plant’s root and transferred to PowerBead Tubes (QIAGEN DNeasy PowerSoil Kit, Germany) as part of the soil niche ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted from cerebellum of WT and Pum1-/-male and female at 10 weeks of age using the miRNeasy kit (QIAGEN, # 217004) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... after which the cells were FACS sorted for high and low reporter signal.The genomic DNA was extracted by lysing the cell pellets for 10 minutes at 56°C in AL buffer (Qiagen, 19075), supplemented with Proteinase K (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted from HCC cells cultured in DMEM + 10% FS for 24 hours using a Qiagen RNeasy Plus Mini Kit (Qiagen #74134) with an additional on column DNA digestion using Qiagen TURBO DNase (Qiagen #AM2238 ...
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bioRxiv - Immunology 2022Quote: ... DNA synthesis reaction was carried out in 100 μl using 10 pmol of each primer with HotStarTaq Plus DNA Polymerase (Qiagen, 203603). Purified dsDNA products were amplified with 10 pmol of each primer in a 100 μl total reaction volume ...
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bioRxiv - Neuroscience 2022Quote: Total RNA for each sample was extracted pooled 10 BPs using the RNeasy Plus Micro kit (catalog # 74004, QIAGEN, Venlo, Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Technical duplicates of gene-specific products were synthesised in 10 µl reactions using the Rotor-Gene SYBR Green PCR kit (Qiagen, UK) on a Rotor-Gene 6000 Real-Time PCR machine (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from 9 AAA and 10 PAD patients’ serum using the miRNeasy Serum/Plasma Advanced Kit (Qiagen, Hilden, Germany), following in principle the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... callus and contralateral intact bone were removed as described previously (10) and immediately homogenized in QIAzol Lysis Reagent (QIAGEN, Valencia, CA), and stored at −80°C ...
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bioRxiv - Immunology 2023Quote: DNA was extracted from 1×10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer’s instructions (Qiagen Hilden, Germany). DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Plant Biology 2022Quote: ... pods from 8-10 different plants were pooled and RNA was isolated using RNAeasy plant mini kit (Qiagen, Germantown, MD, USA). RNA libraries were prepared using standard Illumina protocols and RNA sequencing was performed using NovaSeq 6000 PE150 by Novogene co ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h or 24 h) using RNeasy Kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The sample was then centrifuged at 15000 rpm for 10 min and the pellet was resuspended in 100 μl buffer EB (Qiagen, 19086). For each sample of a 96-well plate ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from 10-d-old seedling roots (40 for each line) using the RNeasy Plant Mini Kit (Qiagen, Germany). cDNA was synthesized using TOPscriptTM RT DryMIX (dT18 ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted DNA by adding 3-5 mg of tail tissue to 150 µl of 10% ChelexR resin (Bio- Rad, Inc.) with 1.4 µl of Proteinase K (20 mg/ml, Qiagen, Chatsworth, CA), incubating at 55°C for 180 min ...
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bioRxiv - Biochemistry 2023Quote: ... buffer containing 100 mM Tris-HCl (pH 8.5), 10 mM TCEP, and 40 mM CAA and crushed using zirconia beads (Tomy, Tokyo, Japan) and TissueLyser (Qiagen, Hilden, German). Proteins were inactivated at 95 °C for 5 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 mL of the culture was immediately treated with a double volume (10 mL) of RNA protect bacteria reagent (Qiagen, Germany) for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA was extracted from 10 ♂’s and ♀’s using Blood & Cell Culture DNA Midi Kit (Qiagen, Cat# 13343) and the sequencing library was prepared using the Oxford Nanopore SQK-LSK110 genomic DNA by ligation kit ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 ng of total RNA was used for first strand cDNA synthesis using miRCURY LNA RT Kit (QIAGEN, Cat. No. 339340). Quantification of the relative miRNAs’ expression level was detected by specific miRCURY LNA miRNA PCR Assays and highly sensitive miRCURY LNA SYBR® Green PCR Kit while adjusting the reverse transcriptase product dilution to 1:30 (QIAGEN ...
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bioRxiv - Cancer Biology 2021Quote: ... His-6X-tagged proteins (30 μg/10 μl beads) in 400 μl of PBS were incubated with Ni-NTA Magnetic Agarose Beads (QIAGEN, Hilden, Germany) at room temperature (RT ...
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bioRxiv - Genomics 2019Quote: DNA extractions were performed using ∼10 g of sediment with the PowerMax Soil DNA Isolation Kit (MO BIO Laboratories, Inc.; Qiagen, Hilden, Germany). To increase the DNA yield ...
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bioRxiv - Genomics 2019Quote: ... 10 μL of each PCR product were pooled and purified using three columns of a MinElute PCR Purification Kit (Qiagen cat # 28004) for a total elution volume of 30 μL ...
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bioRxiv - Plant Biology 2021Quote: ... Lyophilized material was pulverized in a 2ml screw top microcentrifuge tube with a glass bead for 10 minutes at a frequency of 20/s in a TissueLyser (Retsch ballmill, Qiagen, Venlo, Netherlands). The material was AIR prepped and destarched using protocols from Barnes and Anderson (Barnes and Anderson ...
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bioRxiv - Bioengineering 2020Quote: ... The RBD wildtype and mutant proteins of SARS-CoV-2 spike with 10×his tag at N terminal were expressed in HEK 293 and purified with Ni-NTA affinity columns (Qiagen, Hilden, Germany).
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bioRxiv - Neuroscience 2020Quote: ... along with the human NaV β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.X:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...