Labshake search
Citations for Qiagen :
801 - 850 of 980 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from the approximately 20 individual seedlings of 10-day-old plants with the RNeasy Plant Mini Kit (QIAGEN) and rDNase (Macherey-Nagel ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 10000g for 10 minutes and the supernatant was allowed to bind with 1 ml of Ni-NTA beads (Qiagen) for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... bead beating was performed on 100 mg of cecal contents for 10 min using a tissue lyser (Qiagen, Germantown, MD). Remaining steps for DNA isolation were performed as per manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... Genomic DNA for sequencing was prepared from 25 L3 salivary gland pairs or 10 mg embryos (0-6 h AED) using DNeasy Blood and Tissue kit (Qiagen). Each sample was prepared in triplicate ...
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bioRxiv - Biochemistry 2023Quote: ... SUMO protease was added at a final concentration 1/10 and the mixture was dialyzed overnight at 4 °C against the buffer DB6 (50 mM Tris-HCl pH 8, 150 mM NaCl, 10 mM imidazole) and applied on a NiNTA column (Qiagen) equilibrated in the DB6 buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted from the supernatant (12,000 x g for 10 min at 4°C) of centrifuged tissue homogenate using an RNeasy Mini Kit (Qiagen, USA). The extracted total RNA was used for single-strand cDNA synthesis ...
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bioRxiv - Microbiology 2024Quote: ... while samples for RNA-based analyses were mixed at a 1:10 ratio of LifeGuard Soil Preservation (Qiagen, Hilden Germany) before being put on dry ice ...
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bioRxiv - Genetics 2024Quote: Total genomic DNA was extracted from 10% suspension of fin clips (<1 mm2) using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2024Quote: ... qRT-PCR was performed in a 10 μL volume using the miRCURY LNA SYBR Green PCR Kit (Qiagen, Hilden, Germany) and the following miRCURY LNA miRNA PCR primer sets (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... or 10 ul buffy coat (from Accio Biobank or NHS) using the DNeasy Blood and Tissue Kit (Cat# 69504, Qiagen) following the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... The pellets were resuspended with buffer A and loaded onto a 10 mL column of Ni-NTA agarose (Qiagen, Inc.) equilibrated with buffer A ...
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bioRxiv - Microbiology 2022Quote: ... and whole lung tissues were harvested and homogenized in DMEM supplemented with 10% FBS and P/S with TissueRuptor (Qiagen). Virus in the supernatant of lung homogenate was intranasally injected into another BALB/c mice ...
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bioRxiv - Pathology 2023Quote: ... DNA was extracted after routine paraffin embedding (2 sections of 20 μm per sample) from 10 canine tumors (DNeasy Blood & Tissue Kit, Cat. No.69504, Qiagen; Veterinary Science Dept ...
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bioRxiv - Neuroscience 2023Quote: ... freshly sorted cells were pelleted at 500 g for 10 minutes at 4° and then resuspended in RLT Plus buffer (Qiagen). Cells were vortexed and frozen for at least one day at −80° before being thawed on ice and processed for RNA using an RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was performed on 10 ng cDNA aliquots in technical triplicates per biological replicate (three minimum) using QuantitTect SYBR Green PCR kit (Qiagen). Amplification was normalized to SDHA (succinate dehydrogenase complex flavoprotein subunit A) ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining cells were pelleted at 1000 x g for 10 minutes at 4°C and lysed in 350 µL of Buffer RLT (Qiagen) supplemented with 3.5 µL of 2-β mercaptoethanol (#444203 ...
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bioRxiv - Genomics 2023Quote: Five Kellet’s whelk individuals were dissected for the Qiagen and Zymo kit extractions with each sample having a duplicate (10 total extractions conducted using the Qiagen kit and 10 total extractions conducted using the Zymo kit ...
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bioRxiv - Physiology 2023Quote: ... were normalized at 10 ng/µL and the reverse transcription of RNA was done following the manufacturer instructions (miRCURY LNA RT Kit, Qiagen). We added 0.5µl of controls UniSp6 and cel-mir-39-3p to the samples as internal reference to check the efficiency of the reverse transcription and PCR amplification ...
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bioRxiv - Genomics 2023Quote: DNA was extracted from 10-20 mg fresh-frozen postmortem brain tissue using the DNEasy Blood and Tissue kit (cat#: 69504, Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... DNA was digested on the column with 10 μL RNAse free DNase mixed with 70 μL RDD buffer (Qiagen, 79254) for 30 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Biochemistry 2022Quote: ... complexes were either concentrated using a 50 kDa MWCO concentrator and further purified on a Superose 6 Increase 10/300 column (Cytiva) equilibrated in low-salt CSFxB or loaded directly onto Ni2+ agarose (Qiagen), eluted using 300 mM imidazole ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and 1x antibiotic-antimycotic by a TissueLyser II bead mill with 5-mm stainless steel beads (Qiagen). After brief centrifugation ...
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bioRxiv - Genomics 2023Quote: ... Samples were clarified by centrifugation at 2,000 rpm for 10 minutes and subjected to nucleic acid extraction using the cador Pathogen 96 QIAcube HT Kit (Qiagen) in a QIAcube HT automated extractor (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... Following complete removal and evaporation of residual ethanol (10 mins at 30°C) DNA was extracted using the AllPrep DNA/RNA FFPE Kit (Qiagen). DNA was eluted in 40 µl Elution buffer.
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bioRxiv - Biochemistry 2023Quote: ... The pellet was then air dried for 1 minute and re-suspended in 50 μL Buffer EB (10 mM Tris-Cl, pH 8.5; All buffers from QIAprep Spin Miniprep Kit, Qiagen #27104). Purified bacmid was then transfected into Sf9 cells ...
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bioRxiv - Microbiology 2023Quote: RNA was isolated from 2 h and 10 h RPMI-grown and macrophage-internalized Cg cells using the RNeasy kit (Qiagen), followed by DNase I digestion ...
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bioRxiv - Microbiology 2023Quote: ... 2 ml of the culture was harvested and centrifuged at 11,000 × g for 10 minutes where the cell pellet was used for genome extraction with the DNeasy Blood & Tissue Kit (Qiagen) protocols ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from five 10-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Microbiology 2023Quote: ... Metagenomic sequencing libraries were constructed from extracted DNA samples with 10 μL (1/5 volume) reactions using the QIAseq FX DNA Library Kit (QIAGEN). Each metagenomic sequencing library was sequenced using the Illumina NextSeq 2000 System 2 x 150 bp configuration.
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bioRxiv - Microbiology 2023Quote: ... Pellets were washed in 2 mL cold 10 mM NaCl + 4 mL cold RNAprotect Bacteria Reagent (Qiagen Cat. No. 76506) and repelletted at 4,255 x g ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from ∼10–60mg of muscle tissue was extracted using a Qiagen miRNeasy Mini kit (217004, Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the FT and Eluate fractions (90 µL each) were mixed with 10 µL 3 M sodium acetate and applied to a QIAquick spin column (Qiagen). Purified DNA was visualized a 1.3% agarose / 0.5x TBE gel and SYBR Green staining ...
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bioRxiv - Biochemistry 2023Quote: ... Glycerol was added to the supernatant to a final concentration of 10 % and was then incubated with Ni-NTA resin (QIAGEN) for two hours ...
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bioRxiv - Neuroscience 2023Quote: ... 3 μL were used as template in a 10 μL final volume reaction using the miRCURY LNA SYBR Green PCR Kit (Qiagen) in a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from roots of a 14-day-old Col and GFP-GrxS5 plants grown on 10 mM NO3- using RNeasy Mini Kit (QIAGEN). We used 1 μg total RNA for mRNA purification using the NEBNext Oligo d (T ...
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bioRxiv - Developmental Biology 2023Quote: ... Then samples and inputs were incubated for 4 hr at 37°C with 1.5 µl of 10 mg/ml RNase A (Qiagen, 1007885) and 15 µl 10% sodium dodecyl sulfate and 3.5 µl 20 mg/ml Proteinase K (Thermo Fisher ...
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bioRxiv - Physiology 2024Quote: ... A fraction (10%) of the total lysate volume was mixed with 9 volumes of QIAZOL (Cat. # 79306; QIAGEN, Hilden, Germany) and frozen at -80°C ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was isolated from 10-100 mg lyophilized leaf material using the DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany). DNA amount and quality were measured with the QubitTM 4 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The first PCR was conducted in a 10 μL reaction volume per sample using the Multiplex PCR Plus Kit (Qiagen) with primers 515f/806r (Apprill et al. ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 30-70 mg of tissue or 5.0 x 10^6 cells for Source 1 cell lines using commercially available QIAGEN QIAamp Mini Kit (QIAGEN, 51304), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: Total RNA was isolated from 10 third-instar larvae for three biological replicates using an RNeasy Lipid Tissue Mini kit (Qiagen). DNA contamination was removed using TURBO DNA-free (Ambion) ...
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bioRxiv - Cell Biology 2024Quote: ... Thirty 10-μm thickness tissue sections were collected from each tissue explant for RNA isolation using RNeasy Mini Kit (74104, Qiagen). The multiplexed NanoString nCounter™ Immunology Panel (PSTD Hs Immunology V2-12 ...
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bioRxiv - Molecular Biology 2024Quote: ... To assess ribosomal biogenesis total RNA was extracted from ∼10 mg of heart tissue using Qiagen RNeasy Fibrous Tissue Mini Kit (Qiagen). Concentration and quality of isolated RNA were determined by NanoDrop (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: Collected microvessels were pelleted at 20,000 × g for 10 min and resuspended in 350 μl of RLT Plus Buffer from the RNeasy Micro Kit (QIAGEN). RNA was isolated using the RNeasy Plus Micro Kit ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was filtered using a 0.22 μm syringe filter and loaded onto a NiNTA 10/50 column (Qiagen, Germany) and proteins were eluted with an increasing gradient of imidazole ...
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bioRxiv - Genetics 2024Quote: ... DNA samples (70–80 oocytes and 7–10 blastocysts per sample) were subjected to bisulfite conversion through 2 µg carrier RNA (QIAGEN). Nested PCR was performed using EpiTaq HS with the primers listed in Supplementary Table 4 ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was isolated from 10-day-old L19 mutant seedlings by using a DNeasy Plant Mini Kit (Qiagen, www.qiagen.com) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... BALF samples and 10% tissue homogenates were mixed with an equal volume of the RLT buffer (Qiagen, Germantown, MD, USA). Two hundreds microliter of the sample lysate and 200 µL isopropanol was added into the extraction plate ...
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bioRxiv - Zoology 2021Quote: ... PCR amplifications were carried out in 10 μl reaction volumes containing 5 μl Type-it Microsatellite PCR Kit (Qiagen, Hilden, Germany), 0.5 μM of each primer ...