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Citations for Qiagen :
701 - 750 of 980 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... leaf tissues were collected and homogenized in 400 μL of TPS buffer (100 mM Tris-HCl, 10 mM EDTA, 1 M KCl, pH8.0) using TissueLyser II (Qiagen), followed by incubation for 20 min at 75°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Sporophytes and the surrounding maternal calyptra tissue were dissected out of the archegoniophores into 10% RNALater (Qiagen, Hilden, Germany) on Microscope slides with cavities (Marienfeld Superior ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with Angiotensin-II (1 μM) alone or in combination with DAPT (10 μM) or siNotch1 (Qiagen) (50 nM ...
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bioRxiv - Neuroscience 2023Quote: Sixty 10 μm cryosections of quadriceps muscle snap frozen in liquid nitrogen were collected in QIAzole (Qiagen Cat#79306). Four male and four female wildtype and Col6a2-/- mice were included at two different age groups (5 weeks and 25 weeks old) ...
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bioRxiv - Cancer Biology 2022Quote: ... after adding 1.0×10^6 copies/µL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Colonic organoids were stimulated with 10% cCM or iCM for 24h prior to lysis and RNA isolation (Qiagen RNeasy). Quadruplicates were sequenced.
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bioRxiv - Cell Biology 2023Quote: ... Overnight cultures were pelleted at 3,000g for 10 min and plasmid DNA was purified using a Qiagen miniprep kit (27106, Qiagen). Sequences were verified by Sanger sequencing (Eton Bioscience Inc).
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bioRxiv - Systems Biology 2023Quote: ... x mg solid matrix were mixed with five times the μl amount of ACN:water (1:1, v/v) and homogenised with a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After a short centrifugation (2 min ...
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bioRxiv - Microbiology 2023Quote: ... SYBR green real-time PCR assay was carried out in a 20μL PCR mixture volume consisting of 10 μL of 2X Quantitect SYBR green RT-PCR Master Mix (Qiagen) containing HotStarTaq DNA polymerase ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated in lysis buffer (DPBS with 0.5% Triton X-100, 10 mM MgCl2, 5 mM CaCl2, 100 µg/mL RNAse A (Qiagen)) overnight at 37°C in a water bath to allow capsid maturation ...
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bioRxiv - Microbiology 2023Quote: ... for 10 minutes at 37°C followed by a final clean-up with Qiagen RNeasy MinElute Kit (Qiagen, #74204) using default manufacturer’s protocol (which keeps RNAs > 200nt ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg⋅ml –1 Leupeptin and 3 mM Benzamidine) with steel beads at 28.5 Hz for 1 min (Qiagen TissueLyser II ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from 5-10×106 cells using the Gentra Puregene Cell Kit (Qiagen, cat no. 158046) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Plasmid transfections were performed in six-well tissue-culture treated dishes at 1.8 × 10^6 cells/ml using Effectene (301427; QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 9-10 mL of Buffer BB (1/3 of the lysate volume, From QIAGEN Plasmid Plus Midi Kit) was added ...
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bioRxiv - Biochemistry 2024Quote: ... Overnight cultures were pelleted at 3,000 g for 10 min and plasmid DNA was extracted and purified using a Qiagen miniprep kit (27106, Qiagen). Sequences were verified by Sanger sequencing (Eton Bioscience Inc).
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bioRxiv - Microbiology 2024Quote: ... The cells were then incubated in lysis buffer (DPBS with 0.5% Triton X-100, 10 mM MgCl2, 5 mM CaCl2, 100 µg/mL RNAse A (Qiagen)) overnight at 37°C in a water bath to allow capsid maturation ...
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bioRxiv - Genetics 2024Quote: ... we generated 13 pools of 10 FGOs per genotype in a single well of 8-well strips containing 2.5μl of Buffer RLT (QIAGEN; 79216). Samples were stored in a -80C freezer ...
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bioRxiv - Biochemistry 2021Quote: ... Cells with lysis buffer were pulverized in a 10 mL jar containing a 12 mm grinding ball using a TissueLyser II (QIAGEN) by performing 5 rounds of 3 min at 15 Hz ...
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bioRxiv - Cell Biology 2020Quote: ... was evaporated to half volume on a vacuum centrifuge (MAXI-DRY LYO, Heto) and 10 µL of the volume was treated with REPLI-g Mini (Qiagen) for 40 hours.
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: RNA was extracted from appropriately staged embryos or whole larvae (n=10 /sample) using the RNeasy Plus Universal mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and 10 mM imidazole was added to the supernatant then loaded to a gravity column packed with Ni-NTA resins (Qiagen) pre- equilibrated with 20 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... or 1 μl 1:10 diluted oligonucleotides (for RNA from immunopurified ribosomes) from the QIAseq FastSelect –rRNA Yeast Kit (Qiagen) in 10.5 μl total at 75°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted from homogenized whole bodies with a Qiagen DNeasy kit after overnight treatment with 10% proteinase K (Qiagen) at 56 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The tissue was ground to a fine powder by adding 10-15 zirconia beads (1 mm diameter) and using a TissueLyser (Qiagen) for sunflower ligules ...
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bioRxiv - Microbiology 2019Quote: DNA for 16S rRNA gene sequencing was extracted from 10-50 mg fecal pellet from each mouse using the DNeasy Powersoil HTP 96 Kit (Qiagen) and an EpMotion 5075 automated pipetting system (Eppendorf) ...
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bioRxiv - Molecular Biology 2020Quote: ... after adding 1.0 x 10^8 copies/μL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Purified DNA was eluted in either 45 µl of EB buffer (10 mM tris-hydrochloride (pH 8.0) (Qiagen, The Netherlands) or 45 µl of TE buffer (10 mM tris-hydrochloride (pH 8.0) ...
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bioRxiv - Cancer Biology 2020Quote: DNA and RNA were extracted from the cervical cancer tissues (5-10 mg) using the AllPrep DNA/RNA Micro Kit (QIAGEN) as described by the manufacturer ...
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bioRxiv - Neuroscience 2019Quote: RNA extraction from 1-10 x104 FACS-sorted microglia or iPS-derived microglia-like cells was performed on the QIAcube (Qiagen) using the RNeasy Micro Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Each reaction mix comprised 10 μL of 2x Rotor-Gene Multiplex PCR Master Mix (Rotor-Gene Multiplex PCR Kit, catalogue no. 204774; Qiagen), 1 μL of a mix of primers and probes (to give final concentrations of 0.5 mM each primer and 0.2 mM each probe) ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA was extracted from 10 ul of the purified virus using the MinElute Virus Spin Kit (Qiagen Cat#57704), and the viral genome titer was determined by qPCR using an AAV2 rep gene specific primer probe set (repF ...
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bioRxiv - Microbiology 2019Quote: ... All sequences were analysed using the BLAST program [27] and the variable regions were assembled using the CLC Genomics Workbench 10 (QIAGEN). The complete sequence of the integrons were deposited in the GenBank database ...
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bioRxiv - Immunology 2020Quote: ... and DNA from 7-10 clones from before and after FLPo-mediated recombination was prepared by miniprep (Qiagen, cat. 27106) and sequenced by Sanger sequencing with the primer GFP int F Age 66 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 2 to 10 g slices of liver tissue were homogenized in cell lysis solution and proteinase K (Qiagen, Germantown, USA). DNA was extracted from the liver homogenates and resuspended in buffer ...
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bioRxiv - Immunology 2019Quote: Total RNA samples were diluted to a concentration of 10 ng/μl prior to gene expression studies by qRT-PCR using QuantiFast SYBR green RT-PCR kit (Qiagen) in 7900HT Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Physiology 2019Quote: ... 72 °C - 10 min) and amplicons separated using a Qiaxcel Advanced Separation System using 15 - 3000 bp markers (Qiagen, UK).
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bioRxiv - Immunology 2019Quote: ... each fluorescent cell was pipetted up and down the micropipette three times to remove unwanted contamination before being ejected into 10 μl of cold RLT (Qiagen) supplemented with 1% β-mercaptoethanol (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Nuclei pellets were centrifuged for 10 min at 1500g and processed immediately for gDNA extraction using a QIAamp DNA micro kit (Qiagen) according to manufacturer instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Cells attached to the collagen matrix were fixed with 4% PFA for 10 minutes at room temperature for IF analysis or lysed with RLT buffer (Qiagen) for RNA isolation and qRT-PCR analysis as described above.
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bioRxiv - Biophysics 2021Quote: ... PNGase F and the cleaved 10 × His tag were removed by passing the sample through Ni-NTA superflow resin (QIAGEN). The receptor was concentrated to 20–30 mg/ml with a 100 kDa cut-off concentrator (Millipore) ...
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Oligomerization state of the functional bacterial twin arginine translocation (Tat) receptor complexbioRxiv - Biophysics 2021Quote: ... The supernatant was loaded onto a 10 x 1 cm column with 2 mL Ni-NTA Superflow resin (Cat. #30230, Qiagen) that was pre-equilibrated with Buffer A (10 mM CAPS ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from cells collected from 10 mL late-exponential phase culture in Todd-Hewitt broth with the DNeasy Blood & Tissue kit (Qiagen) using a modified protocol with an additional lysis step as described previously 61 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded at 50% confluency in a 10 cm dish and transfected with 200 pmol siRNA (Qiagen, Germantown, MD) using RNAiMax Lipofectamine (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Two days after infection the mice were sacrificed and the distal 10 cm of the small intestine was dissected and shortly incubated in RNA later (Qiagen). Then the tissue was flushed with ice-cold DPBS to remove the fecal content and opened longitudinally on ice using scissors ...
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bioRxiv - Genomics 2021Quote: ... a 1.0 μL aliquot of each sample was amplified in 10 μL reactions incubated at 30°C for 16 h as described in the REPLI-g Mini Kit (QIAGEN).
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from vpr+ and vpr- cells 10 days post-infection using Qiagen DNeasy Blood & Tissue kit (Qiagen) and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro- TUBE according to the manufacturer’s recommended settings (Covaris ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from cells (6-well dishes) or liver pieces (∼10-15mg) using the RNeasy Plus Mini kit (Qiagen). For livers ...