Labshake search
Citations for Qiagen :
601 - 650 of 980 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... in a 20 μL system with a mixture of 10 μL 2×SYBR Premix ExTaq (Qiagen, Germany), 2.0 μL diluted cDNA or double distilled water as a negative control ...
-
bioRxiv - Cell Biology 2021Quote: ... and 10 mg/mL proteinase K) and DNA clean up (MiniElute reaction clean up kit, 28204, Qiagen). qPCR was performed using mPer2 and Wnt3a primers ...
-
bioRxiv - Genomics 2022Quote: ... powdered liver (~10 mg) was resuspended in 900 μL QIAzol (RNeasy 96 Universal Tissue Kit, Qiagen, 74881) and homogenized at 25/s for 1 min at 4°C using a TissueLyser II (Qiagen ...
-
bioRxiv - Genetics 2022Quote: ... of which 2.5 μL was used for 10 μL reactions using the RotorGene Q qPCR system (QIAGEN). Reactions were performed with 5 min at 95 °C ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... DNA was eluted in 45 µl of EB buffer (10 mM tris-hydrochloride, pH 8.0; QIAGEN, Netherlands) supplemented with 0.05% (v/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 sections were stored in -80°C prior to RNA extraction using the Rneasy micro kit (Qiagen). RNA quality was assessed using a 5300 Fragment Analyzer (Agilent ...
-
bioRxiv - Genomics 2022Quote: ... distilled water to the membrane and ground the reaction for 10 minutes on the TissueLyser II (Qiagen) at 32 Hz ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris pH 8.5, 2 mM MgCl2, 0.5% NP40, 0.5% Tween20, 0.05 U/mL QIAGEN protease) and following the procedure described in (42) ...
-
bioRxiv - Cancer Biology 2023Quote: ... were transfected with 10 nM Malat1 ENE-targeting (ASO) or control (Con) steric blocking ASOs (Exiqon, Qiagen) using Lipofectamine 2000 (ThermoFisher Scientific) ...
-
bioRxiv - Genomics 2023Quote: Approximately 10 mg of each frozen tissue sample was homogenized in 1ml QIAzol Lysis Reagent (79306, QIAGEN) using BeadBug 6 Microtube Homogenizer (D1036 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and genomic DNA from 10 SPF shrimp was isolated using the DNeasy blood and tissue kit (Qiagen). LAMP reactions were prepared as detailed in Table S2a and run at 60°C and 62°C (TSV and WSSV ...
-
bioRxiv - Genomics 2023Quote: ... 10 CNP) from foot tissue were performed using DNeasyBlood and Tissue DNA extraction kit (Qiagen Cat.no. 69504) following the manufacturer’s recommendations (Elution in 110 μl elution buffer) ...
-
bioRxiv - Microbiology 2023Quote: ... tissues collected were weighed and homogenized in PBS/10% FBS using the TissueLyser (Qiagen; Retsch, Haan, Germany). After clarification by centrifugation ...
-
bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from 10 ml cultures of yeast strains and purified with an RNeasy kit (Qiagen). The RNA was prepared for sequencing using the TruSeq Stranded Total RNA Sample Prep adaptor kit (Illumina) ...
-
bioRxiv - Genetics 2020Quote: ... denaturation solution (0.2M NaOH), and wash buffer (10 mM Tris-acetate, pH 7.6) using the PyroMark Q96 Vacuum Workstation (QIAGEN). Purified DNA resuspended in annealing buffer (20 mM Tris-acetate pH7.6 ...
-
bioRxiv - Genomics 2019Quote: gDNA was extracted from 10-100 μL of buffy coat using the DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s protocol with two elutions of buffer AE (100 μL per elution) ...
-
bioRxiv - Molecular Biology 2019Quote: ... resuspended in 50 mM sodium citrate buffer containing 10 µl of RNase A (20 mg/ml, Qiagen 76254) and incubated for 2 hours at 50°C ...
-
bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from the gut samples (7-10 guts/sample) using the RNeasy Mini kit (Qiagen) and the on-column DNase I treatment (79254 ...
-
bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 10 µL of mouse sera using QIAamp® viral RNA Mini Kit (Qiagen) or about 20 mg of organs homogenised by Precellys Lysing Kit Hard tissue grinding (Bertin Technologies ...
-
bioRxiv - Genomics 2021Quote: ... single cell directly into 2 µl lysis buffer (30 mM Tris-HCl [pH = 8.0], 10 mM NaCl, 0.2 µL Proteinase K [Qiagen, #19133] ...
-
bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
-
bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
-
bioRxiv - Cell Biology 2019Quote: Gene silencing in HEK293 cells was carried out via transient transfection of 10 nmol siRNA (NAV3_siRNA1: SI04272646, NAV3_siRNA2: SI04366215) or scrambled control (Qiagen) using Lipofectamine 3000 (ThermoFisher) ...
-
bioRxiv - Physiology 2021Quote: Total liver RNA was extracted from n=10 mice using a RNeasy Plus mini kit (Qiagen, Hilden, Germany). Total gonadal adipose RNA was extracted using a modified Tri-reagent (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2021Quote: ... and adjacent normal tissues from 10 ICC patients were isolated using a RNeasy Mini Kit (Qiagen, Hilden, Germany). Strand-specific libraries were prepared using a TruSeq Stranded Total RNA Sample Preparation kit (Illumina ...
-
bioRxiv - Molecular Biology 2019Quote: ... 10-15 μl of primary or re-amplified WTA product was purified using QIAquick PCR Purification Kit (QIAgen) according to the manufacturer’s instruction with several changes ...
-
bioRxiv - Plant Biology 2020Quote: ... Roots were washed quickly (< 1 min) to detach the surrounding peat matrix using 10% RNAlater (QIAGEN, Valencia, CA) in 1X PBS as the capture buffer to mitigate RNA degradation ...
-
bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from 10-day-old Arabidopsis seedlings with an RNeasy® Plant Mini Kit (QIAGEN) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... 120 µL of elution buffer of elution buffer II (10 mM Tris pH 8.0, 1 mM EDTA, 0.67% SDS) and 7 µL Proteinase K (Qiagen) was added ...
-
bioRxiv - Clinical Trials 2019Quote: Genomic DNA from 1-10 Million CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen). In some experiments ...
-
bioRxiv - Cell Biology 2021Quote: ... the platelets were pelleted at 1000 x g for 10 minutes in the presence of prostacyclin (PGI2, 2μM) and re-suspended in 500μl of Qiazol (QIAGen).
-
bioRxiv - Genomics 2021Quote: ... Nuclear DNA was extracted individually from 10 mg of tissue using the DNeasy Plant Mini Kit (QIAGEN, DE) following the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... approximately 10 mg (wet weight) of both harvested cultures were homogenized by using a TissueLyser LT mill (Qiagen) and extraction was performed by chloroform and methanol solution (ratio – 2:1 ...
-
bioRxiv - Genomics 2020Quote: ... Individual cells were sorted into the wells of 96-well plates with 10 μL TCL buffer (Qiagen, #1070498) with 1% beta-mercaptoethanol (Sigma 63689 ...
-
bioRxiv - Neuroscience 2022Quote: ... canonical pathways were generated through the use of Ingenuity Pathway Analysis (IPA, Release Date: 2021-10-22, Qiagen).
-
bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted from pooled individuals (n = 10) using a DNeasy® Blood & Tissue kit (Qiagen, Hilden, Germany). A continuous long-read library was prepared and sequenced using PacBio Sequel II technology by Berry Genomics (Berry Genomics Co ...
-
bioRxiv - Microbiology 2022Quote: RNA was extracted from 5-10 snap-frozen larvae with the RNeasy Mini kit (Qiagen cat no. 74104) and reverse-transcribed using QuantiTect Reverse Transcription kit (Qiagen cat no 205311 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Up to 10 consecutive slides per patient were micro-dissected and collected in 350µl of RLT buffer (Qiagen). The sample was inverted and centrifuged at 13.000rpm for 5 min and stored at −80°C ...
-
bioRxiv - Genomics 2022Quote: ... DNA fragments were released by addition of 15 µL 10% SDS and 30 µL Proteinase K (Qiagen, #19133) to each tube followed by incubation at 50°C for 3h ...
-
bioRxiv - Molecular Biology 2022Quote: Whole RNA was extracted from over 1 × 10^5 cells using the QIAGEN RNeasy Micro kit (QIAGEN, 74004) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from 50 μl of 10% suspension using an RNeasy Plus kit (Qiagen, Hilden, Germany). For PCR-positive samples ...
-
bioRxiv - Neuroscience 2024Quote: RNA from immunopanned astrocytes or neurons from 10-month hCOs was isolated using RNAeasy Micro Kit (Qiagen, 74004). Bulk RNA sequencing libraries were prepared using the NEBNext Ultra II kit with PolyA Selection and sequenced using the Illumina HiSeq 4000 System to a minimum of 30 million paired-end reads per sample ...
-
bioRxiv - Molecular Biology 2023Quote: ... genomic DNA from 10 adult flies (5 males/5 females) was extracted using DNeasy Blood & Tissue Kit (Qiagen). DNA was resuspended and sheared in 1X TE (0.1 mM EDTA ...
-
bioRxiv - Microbiology 2023Quote: ... A 5 ml aliquot of culture was removed and added to 10 ml of RNAprotect Bacteria Reagent (Qiagen) and mixed by vortexing ...
-
bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 5-10 g of soil per sample with the DNeasy PowerMax Soil Kit (Qiagen) and used for short read and long read sequencing ...
-
bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA was extracted from 10 mg homogenized fresh frozen tumors using DNeasy® Blood & tissue kit (Qiagen). Subsequently ...
-
bioRxiv - Cell Biology 2023Quote: ... and transfected with 10 ng/well of dsRNA targeting the candidates using the Effectene Transfection reagent (301427, QIAGEN). dsCtl and Dynasore (D7693 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Total cell-free DNA from urine supernatants was extracted from a median of 3.6 mL urine supernatant (range 0.7–3.65 mL) incubated with 10% ATL buffer before purification using the QIAsymphony DSP Circulating DNA Kit (Qiagen).
-
bioRxiv - Cell Biology 2023Quote: ... 10 minutes 4°C and the supernatant was incubated with pre-equilibrated Ni-NTA agarose beads (Qiagen, #1018244) with shaking on ice for 3 hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... 4 sections 10 µm thick were placed in a chilled Eppendorf tube and RNA extraction protocol from Qiagen was performed (Rneasy FFPE Kit 73504 ...