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Citations for Qiagen :
651 - 700 of 980 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... 30,000 transformants was pooled and purified by splitting the entire pellet over 10 Miniprep columns (QIAGEN, Hilden, Germany) and eluting each in 30 μl of EB Buffer ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from 10-15 mg of powdered frozen heart tissue (RNeasy Fibrous Tissue Mini Kit, Qiagen) and used for cDNA synthesis (QuantiTect Reverse Transcription Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA from 10 adult flies (5 males/5 females) was extracted using DNeasy Blood & Tissue Kit (Qiagen). DNA was resuspended and sheared in 1X TE (0.1 mM EDTA ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 sections 10 µm thick were placed in a chilled Eppendorf tube and RNA extraction protocol from Qiagen was performed (Rneasy FFPE Kit 73504 ...
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bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
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bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and and concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted 96h after transfection using homemade cell lysis buffer (10 mM Tris-HCl pH8.5 and 0.05% SDS) or DNeasy Blood & Tissue Kits (Qiagen) to evaluate editing frequencies ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 µM each of forward and reverse primers (listed in Supplemental information) on Rotor-Gene Q (QIAGEN). The thermal cycling conditions included an initial denaturation step at 95°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction for sediment samples was carried out using DNeasy PowerMax Soil DNA Extraction Kit (QIAGEN 12988-10, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... 0.4 M NaOH and 10 mM Tris-acetated (pH 7.6) and then hybridized to the sequencing primer in PyroMark annealing buffer (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... while method-3 was a modified protocol of the DNeasy PowerMax Soil Kit (Cat.No. 12988-10) provided by Qiagen (based on communication exchanged with the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... CIN3 DNA and RNA were prepared using 10 µm sections from the patient’s archived CIN3 FFPE block and the RNeasy Plus Universal Kit (Qiagen), respectively ...
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bioRxiv - Cancer Biology 2019Quote: RNA was extracted from 10-15mg of frozen tumour tissue using the RNeasy Plus Kit according to the manufacturer’s protocol (Qiagen). cDNA synthesis was carried out using QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... DNA from chiggers and 10 μl of water bath samples were extracted using the DNeasy Blood & Tissue Kit (Qiagen).
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bioRxiv - Microbiology 2019Quote: ... pestis extracts were eluted in 75μl of Elution Buffer (EB) (10 mM Tris-Cl, pH 8.5, Qiagen, Valencia, CA) and B ...
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bioRxiv - Plant Biology 2019Quote: ... Freshly extracted mRNA from 10 aphids was converted into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany) and 40 ng of cDNA was used as the template for qRT-PCR in an Applied Biosystems 7500 FAST real-time PCR system ...
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bioRxiv - Bioengineering 2019Quote: ... genomic DNA (gDNA) was extracted from 10 transgenic fourth instar larvae using the DNeasy Blood & Tissue Kit (Qiagen #69504) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Each sample was normalized to contain 10 ng/μl DNA using QIAgility Liquid Handling System (Qiagen, Germantown, Maryland, USA) for library construction ...
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bioRxiv - Cell Biology 2021Quote: RNA from 5-10 million NK cells treated overnight with indicated conditions was extracted using Rneasy Plus kit (Qiagen). Samples were prepared for Illumina sequencing following the manufacturer’s protocol using the TruSeq® stranded total RNA with rRNA depletion using Ribo Zero TM Human/Mouse/Rat ...
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bioRxiv - Cell Biology 2021Quote: Tissue lysates were further homogenized and centrifuged at 10,000 g for 10 min at 4°C in microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate ...
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bioRxiv - Microbiology 2021Quote: ... The centrifugation step was repeated and the pellet air-dried before the DNA was dissolved in 10 mM Tris-HCl (pH 8.5, Qiagen) at 65 °C for 20 min ...
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bioRxiv - Systems Biology 2020Quote: ... The sealed plates were subjected to 10 minutes of bead beating at 20 Hz using an (Qiagen Tissuelyser II). After bead beating ...
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 14,000 × g for 10 min and RNA was extracted using Qiagen RNeasy mini plus kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from platelet pellets (10 million per population) using the RNeasy Mini kit according to manufacturer’s instructions (Qiagen). cDNA was synthesized from extracted platelet RNA using SuperScript® III first-strand synthesis system for RT-PCR (Life Technologies) ...
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bioRxiv - Biochemistry 2022Quote: ... 10% glycerol and 1 mM DTT) and recombinant TrcP was purified from clarified lysates using Ni-NTA resin (Qiagen) and gravity chromatography ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs or its variants (10 µg) were bound to 4 µl of Ni-NTA Magnetic Agarose Beads (Qiagen) in 50 µl interaction buffer (50 mM Tris pH 9.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Embryos and the surrounding maternal calyptra tissue were dissected out of the archegoniophore into 10% RNALater (Qiagen, Hilden, Germany) on Microscope slides with cavities (Marienfeld Superior ...
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bioRxiv - Plant Biology 2022Quote: ... Sections were immediately placed into 96-well plates (on ice) containing 10 μL of RLT plus (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% ethanol ...
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Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... parasites were spun at 1300g for 10 minutes and resuspended in buffer RLT from the RNeasy Mini Kit (Qiagen). Genomic DNA was removed from the sample using TurboDNase (Thermo ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from the sediment samples using the larger format PowerMax Soil DNA Isolation Kit (12988-10, QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and 3.5 ml of RLT buffer with B-mercaptoethanol (10 µl for every 350 µl of RLT buffer – Qiagen) was added ...
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bioRxiv - Physiology 2022Quote: 10 mg of liver were used to extract DNA with the DNeasy Blood & Tissue Kit (QIAGEN, cat. no. 69506), according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2021Quote: Total genomic DNA was extracted from 10 coastal plant species (Table S2) using the Plant DNeasy Mini Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... SYBR green RT-qPCR was performed in triplicate with 10 ng of template cDNA using QuantiTect Master Mix (Qiagen) on a 7900-HT Fast Real-time System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2020Quote: ... the allantois was dissected out and lysed in 10 μl DirectPCR (Tail) digest reagent (Qiagen, catalog no. 102-T) supplemented with 1 μl of proteinase K overnight at 55°C ...
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bioRxiv - Genomics 2022Quote: ... 10 budgerigar samples and 12 zebra finch samples) using the DNeasy® Blood & Tissue Kit (Cat. No. 69581, Qiagen). For PCR ...
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bioRxiv - Microbiology 2020Quote: DNA for metagenomic sequencing was extracted from ~7 g sediment (~0.7 g sediment in 10 individual lysis tubes) using PowerSoil DNA Isolation Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... We eluted purified DNA in 45 µl of EB buffer (10 mM tris-hydrochloride (pH 8.0) (Qiagen, The Netherlands) supplemented with 0.05% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Second-strand synthesis reaction was carried out in 100 μl using 10 pmol of each primer (HotStarTaq Plus, Qiagen). Residual primers and dNTPs were again degraded enzymatically (ExoSAP-IT ...
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bioRxiv - Immunology 2021Quote: ... or ectodomain buffer (20 mM Tris, pH 8.5, 500 mM NaCl) and applied to 10 ml Ni-NTA-Sepharose (Qiagen) columns ...
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bioRxiv - Microbiology 2021Quote: ... Samples were spun at 5000 x g for 10 minutes and supernatant was discarded before resuspension in 1.5 mL QIAzol reagent (Qiagen). Screw-cap tubes were prepared for each sample by adding ∼250 µL of sterile beads and the QIAzol suspension mixture was added and incubated for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was lysed with a lysis buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 20 mM EDTA, 1% SDS) and proteinase K (Qiagen) was added to samples to degrade proteins ...
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bioRxiv - Systems Biology 2022Quote: ... the bacteria cells were scraped and centrifuged at 3,000g for 10 min and the plasmids were then extracted (Qiagen). Cloning of individual sgRNA base editor construct was based on similar procedure with reduced reagent input.
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was loaded onto a 10 mL Ni-NTA Superflow cartridge (connected two 5 mL cartridges) (30761, Qiagen). The cartridge was washed with 100 mL R buffer containing 200 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... QT65005) and 10 µM each of forward and reverse primers (listed in Table S2) on Rotor-Gene Q (QIAGEN) with PCR procedures under the following programme ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The lysate was centrifuged at 50,000×g for 10 min and to the supernatant 1 mL of 50% Ni−NTA (Qiagen) was added and incubated for 1 h at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA quality was estimated through total RNA extraction from 10 tissue sections with a RNeasy Plus Mini kit (Qiagen). Thereafter RNA integrity number (RIN ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from approximately 5-10 million cells from each organ using the RNeasy Mini Kit (QIAGEN). SuperScript® IV Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Plasmid transfections were performed in six-well tissue-culture treated dishes at 1.8 × 10^6 cells/ml using Effectene (301427; QIAGEN) following the manufacturer’s instructions ...