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Citations for Qiagen :
551 - 600 of 980 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted using the RNeasy Mini (48-hour) RNeasy Micro (10-hour) kit (Qiagen). cDNA was synthesized using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA) ...
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bioRxiv - Immunology 2020Quote: ... Single neutrophils were sorted using an SH800 cell sorter (Sony) into 10 μL TCL buffer (Qiagen) with 1% β-mercaptoethanol (BME ...
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bioRxiv - Immunology 2021Quote: ... Lungs and brain were homogenised in PBS (10% w/v) using a rotor-star homogeniser (Qiagen) and total RNA extracted using TRIzol (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Data were analysed with CLC Genomics Workbench Software v.10 (Qiagen Bioinformatics, Redwood City, CA, USA) and the NCBI database with the BLAST tool (https://blast.ncbi.nlm.nih.gov/Blast.cgi).
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL of 10 mg/mL RNase A (DNeasy Blood &Tissue kit, #69504, Qiagen, Hilden, Germany) was added to each sample and incubated at 37°C for 2 h ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from 10 homogenized bodies using the RNeasy Minikit with DNase treatment (Qiagen) and reverse transcription was performed using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Physiology 2024Quote: ... The 40 µl reaction mix consisted of 10 µl of 4x QIAcuity Probe PCR Kit (QIAGEN), 2 µl of the target assay ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from approximately 10–20 seedlings using the RNeasy Plant Mini Kit (QIAGEN) followed by DNase I treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were eluted in 40 ml of elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5) with optional 15 mins of incubation at 37°C to recover long molecules ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from PBMCs of 10 Holstein Friesian cattle using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA from 10 SPF shrimp was isolated using the RNeasy blood and tissue kit (Qiagen) and genomic DNA from 10 SPF shrimp was isolated using the DNeasy blood and tissue kit (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was isolated from ~10 mg frozen tissue using the DNeasy Blood & Tissue Kit (Qiagen).
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from 10 OD600nm units of yeast cells by RNeasy kits (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and from 10-25mgm of snap-frozen tissue using the DNeasy Blood and Tissue kit (Qiagen) as previously described (21 ...
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bioRxiv - Molecular Biology 2023Quote: Sperm DNA was extracted from ∼ 10 million spermatozoa/sample by lysing cells with RLT+ buffer (Qiagen) with 1% Beta-mercaptoethanol for 15 min. ...
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bioRxiv - Systems Biology 2024Quote: Total RNAs were extracted from approximately 10 mg of liver using RNeasy Mini Kit (74106, QIAGEN) and QIAshredder (79656 ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs from pistils stage 10 or stage14 were isolated with RNeasy Plant Mini Kit (Qiagen). First-strand cDNA was then synthesized using a Transcriptor First Strand cDNA Synthesis kit (Roche) ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from ~10 g of thawed soil using Powermax Soil DNA extraction kit (Qiagen) with some minor modifications as follows ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We performed PCR in a 10 µL volume containing 1x multiplex PCR master mix (Qiagen Hilden, Germany), 5-20 ng of genomic DNA ...
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bioRxiv - Bioengineering 2020Quote: ... The ligated DNA was purified and concentrated into 10 μL dH2O via MinElute PCR Purification Kit (Qiagen). The plasmids were transformed into NEB 5-alpha Electrocompetent E ...
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bioRxiv - Biochemistry 2021Quote: Total RNA was extracted and DNAse treated from a 10-cm dish with RNeasy Kit (Qiagen, Germany), according to manufacture instruction ...
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bioRxiv - Biochemistry 2020Quote: ... cells were transfected in 10 cm2 or 6-well plates at ∼60% confluency using PolyFect (Qiagen 301107) and washed with complete media 6 h later.
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bioRxiv - Cancer Biology 2021Quote: Whole RNA (1-3 μg total per 10 μL volume) was isolated using RNAeasy mini kit (QIAGEN) or TRIzol (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested by centrifugation for 10 min at 3200 x g and resuspended in RNAprotect (Qiagen). RNA was isolated using the RNeasy mini kit (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... the whole CNS was dissected from the snails (n=10) and homogenized using a TissueLyser LT (QIAGEN) in TRI reagent (#93289 ...
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bioRxiv - Molecular Biology 2021Quote: ... and ~10 mg was obtained for DNA isolation using the commercially available DNeasy Blood & Tissue Kit (Qiagen; Venlo ...
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bioRxiv - Microbiology 2019Quote: Bacterial DNA was isolated from 10 mL of urine using the QIAamp Viral RNA Mini Kit (Qiagen) according to manufacturer’s recommendations ...
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bioRxiv - Genomics 2022Quote: ... The samples were cooled to room temperature and 30 μL of 10 mg/mL RNase A (QIAGEN) was added and the samples were incubated at 37°C for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... 8 to 10 independent PCR reactions were pooled together and purified using the PCR purification kit (Qiagen). Multiplexed libraries were sequenced on Illumina HiSeq 2500 to obtain 100 bp single-end reads ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of a 10 min bead beating step at 50 s−1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure-Bacteria kit protocol (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was purified using either 10 mL nickel-chelating column or Strep-Tactin column (Qiagen). The N-terminal His-SUMO tags were cleaved from the eluted protein by TEV protease overnight and removed by passing through a second nickel column ...
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bioRxiv - Plant Biology 2021Quote: ... genomic DNA (gDNA) was extracted from 10-day-old seedlings with the DNeasy plant mini kit (Qiagen) per manufacturer’s introduction ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR reactions were performed in 10 µl volume with 5 µl Qiagen multiplex PCR mixture (Qiagen, Germany), 0.2 mg/ml Bovine Serum Albumin (BSA) ...
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bioRxiv - Neuroscience 2020Quote: ... 10 ng of bisulfite converted DNA was run in a PCR using the PyroMark PCR kit (Qiagen) and the following PCR primers ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 mM EDTA) followed by ethanol precipitation (<500 000 cells) or with QIAamp DNA Mini Kit (Qiagen) (>500 000 cells) ...
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bioRxiv - Cancer Biology 2019Quote: ... and contaminating DNA was removed by DNase treatment for 10 minutes and column purification (Qiagen RNeasy #74106). Pancreas tissue portion for RNA purification was consistently collected from the tail of the organ and immediately cut into smaller pieces and immerse in RNAlater stabilization solution (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... Mechanical disruption of microbial cells was done using TissueLyser II (QIAGEN, 30 shakes/sec., 10-20 min.) and the samples were centrifuged (10,000 g ...
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bioRxiv - Immunology 2020Quote: ... in purity mode into 96-well microplates containing 10 μL of 1% 2-mercaptoethanol RLT buffer (Qiagen) and stored at −80°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and homogenized twice at 10 s at 2 M/s with a 5-mm steal bead (Qiagen) using a tissue homogenizer (MP Biomedicals) ...
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bioRxiv - Immunology 2021Quote: ... The samples (N=3) were collected immediately and soaked in 10 volumes of RNAlater (Qiagen, Hilden, Germany), for sequencing using Illumina (New England Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 embryos were used for genomic DNA isolation using the Qiagen DNAeasy Blood & Tissue kit (Qiagen #69504). DNA was PCR amplified with Promega GoTag green mastermix (Promega #M7123) ...
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bioRxiv - Cell Biology 2020Quote: ... All 10 PCRs of a single sample were pooled and purified using QIAquick PCR Purification Kit (Qiagen). The purified PCR product was then separated using 2% agarose gel and gel extracted with the Qiagen Gel Extraction Kit ...
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bioRxiv - Genomics 2021Quote: ... 43 Banfora-S mosquitoes and 10 Banfora-O mosquitoes using a Qiagen DNeasy Blood & Tissue Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... ∼ 10 mg of cryopreserved liver and spleen tissue samples were ground using a TissueRuptor (Qiagen, Hilden, Germany) in liquid nitrogen and subsequently homogenized in HB Buffer (see buffer recipes in Corces et al ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from ~ 10 g of thawed soil using Powermax Soil DNA extraction kit (Qiagen) with some minor modifications as follows ...
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bioRxiv - Biochemistry 2021Quote: Total RNA was isolated from 10 homogenized zebrafish embryos per sample using the RNeasy Mini Kit (Qiagen). RNA concentration was measured on a spectrophotometer (DeNovix DS-11 FX+) ...
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bioRxiv - Genetics 2020Quote: ... cell pellets (10*106 cells) were collected and RNA was extracted using the RNeasy Mini Kit (Qiagen). RT-PCR was performed using SuperScriptIII Reverse Transcriptase (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was supplemented with 10 mM imidazole and incubated with 1 ml Ni-NTA resin (Qiagen) for 1 hour at 4 °C ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA from 10 Ag(PMB)1 individuals was extracted using the Blood and Tissue Kit (Qiagen). For each sample ...