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Citations for System Biosciences :
401 - 450 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... and concentrated using PEG (System Biosciences, LV825A-1) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
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bioRxiv - Cancer Biology 2022Quote: ... with pPACKH1 vectors and SORE6-dsCopGFP plasmid DNA (kindly provided by Wakefield Lab, NIH) according to the manufacturer’s protocols (System Biosciences). Viral supernatant was collected at 48 hrs ...
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bioRxiv - Developmental Biology 2022Quote: ... The CRISPR gRNA (5pg per embryo) and Cas9 mRNA (150pg per embryo, System Biosciences CAS500A-1) were injected into single-cell embryos ...
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bioRxiv - Molecular Biology 2022Quote: ... The siRNAs for CLC3 and CLC7 were constructed using double-promoter pFIV-H1/U6 siRNA cloning and expression vectors (System Biosciences, Palo Alto, CA, USA; Cat: SI111A-1), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... and the Super piggyBac Transposase expression vector (SBI System Biosciences, PB200PA-1,) using the Lipofectamine™ LTX Reagent with PLUS™ Reagent (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... pmLF were cultivated in 175 cm2 flasks in DMEM supplemented with EV-free FBS (System Biosciences, Palo Alto, CA, USA). Importantly ...
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bioRxiv - Developmental Biology 2022Quote: ... The gRNA (5 pg/embryo) and mRNA encoding Cas9 protein (150 pg/embryo, System Biosciences) were injected into one-cell stage embryos ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).
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bioRxiv - Molecular Biology 2022Quote: ... The lentivirus was concentrated by PEG-it Virus Precipitation Solution (System Biosciences) and stored in –80°C freezer.
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bioRxiv - Immunology 2022Quote: ... EF1a-RFP-H1-gRNA vector (CASLV502PA-R from System bioscience) was modified to introduce BsaI cut site and single sgRNAs were cloned into it by using Golden Gate Assembly kit (BsmBI-v2 ...
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bioRxiv - Cancer Biology 2021Quote: ... the medium was replaced with DMEM without FBS for 1 hour before the plasmids pCDH-GAL-9-T2A-Puro (System Biosciences, CD527A-1) or pLKO ...
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bioRxiv - Cancer Biology 2021Quote: ... Media containing virus was collected both 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Virus Precipitation Solution (System Biosciences, LV810A-1). To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Full-length EXOSC1 was cloned into the Xba I and Nhe I sites of the lentivirus vector pCDH-CMV-MCS-EF1-Puro (System Biosciences, Mountain View, CA, USA) to construct pCDH-Flag EXOSC1 ...
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bioRxiv - Cancer Biology 2021Quote: ... which were generated following the manufacturer’s protocol (System Biosciences, Mountain View, CA, USA). The resultant cells were selected with puromycin for 2 weeks ...
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bioRxiv - Cell Biology 2021Quote: ... into XPack CMV constructs (System Biosciences). The cyclin D1-GFP plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and GFP from pEGFP-N1 (BD Biosciences Clontech ...
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bioRxiv - Cell Biology 2021Quote: ... into XPack CMV constructs (System Biosciences). For cyclin D1 overexpression ...
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bioRxiv - Cell Biology 2021Quote: ... into the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector (System Biosciences, Palo Alto, CA). The cyclin D1-APEX plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and APEX from pcDNA3 APEX-nes (Addgene ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to create a stable line using Piggybac transposase (System Biosciences). A total of 500,000 cells were transfected using the using the mouse neural stem cell nucleofection kit (Lonza ...
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bioRxiv - Immunology 2021Quote: ... concentrated using Peg-it solution (System Biosciences) and titrated on HEK293T cells ...
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bioRxiv - Immunology 2021Quote: ... 5’ ATACGCGGATCCtcagtagggccgccacac 3’ and cloned into a commercial lentivector containing a GFP reporter gene (pCDH-GFP-Em-CD513B-1 from System Biosciences). To overexpress RUNX2 in human T cells ...
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bioRxiv - Genetics 2021Quote: ... This plasmid was derived from the MN530A-1 (System Biosciences) by replacing the GFP Reporter sequence with a multiple cloning site using the restriction enzymes XmaI and StuI and duplexed donor oligonucleotides #2020 and #2021 (Supplemental Table 1) ...
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bioRxiv - Developmental Biology 2021Quote: ... Lentivirus packaging was provided by System Biosciences (Palo Alto, CA). Titers of VSV-G pseudotyped viral particles were ~107 IFUS/mL.
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bioRxiv - Microbiology 2021Quote: ... tHF cells stably transduced with the IFN-responsive pGreenFire-ISRE lentivector (System Biosciences) were further stably transduced with the lentiCRISPRv2 vector ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon was cloned into a modified version of the self-inactivating lentiviral vector pPS-EF1α-GFP-RFP (System Biosciences, LV603PA-1) where GFP and RFP had been removed and replaced by an hPGK-Puromycin cassette (pSIN-EF1a promoter-BGH polyA-hPGK-Puromycin) ...
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bioRxiv - Developmental Biology 2021Quote: ... 8 pl of a mixture containing 500 ng/μl RFP-Piranha mRNA (System Biosciences) and 0.1 mg/ml anti-Tdrd3 antibody (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... a commercially available specific primer set was used (System Biosciences). For the detection of some other targets ...
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bioRxiv - Neuroscience 2021Quote: ... Lentivirus packaging was provided by System Biosciences (Palo Alto, CA). Titers of VSV-G pseudotyped viral particles were ∼107 IFUS/mL.
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bioRxiv - Molecular Biology 2021Quote: ... EVs were isolated from tissue culture media using a commercial kit containing a polyethylene glycol (PEG) solution (ExoQuick-TC ULTRA kit, EQULTRA-20TC-1; System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... This plasmid was co-transfected with piggyBac Transposase expression vector (System Biosciences, PB210PA-1) into the PABPC1-AID cell line (sC152-C16) ...
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bioRxiv - Molecular Biology 2021Quote: ... exosomes from ASCs were labeled fluorescent red using an ExoGlow-Protein EV Labeling Kit (System Biosciences) and then incubated with precipitation solution at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... mRuby was amplified from PB-EF1α-MCS-IRES-RFP (System Biosciences PB531A-2) by PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) 3’-UTR sequence was amplified from the CD813A-1 (System Biosciences) vector.
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bioRxiv - Molecular Biology 2021Quote: The primary human T cell transductions were done with multiple viral titers using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Frozen primary human T cell pellets were thawed and then stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell) for 48 hours and then transduced with various CAR viruses using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) as described above ...
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bioRxiv - Molecular Biology 2021Quote: ... and was cloned into a modified CD813A vector (System Biosciences) using the In-Fusion® HD Cloning Kit (Takara) ...
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bioRxiv - Molecular Biology 2021Quote: ... filtered and concentrated using PEG-it Virus Precipitation Solution (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The viruses were then concentrated using PEG-it Virus Precipitation Solution (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... along with 1/10 total plasmid mass of Super PiggyBac Transposase Expression Vector (System Bioscience, Inc. #PB210PA-1). Stable integrants were selected with 1-2 μg/mL puromycin.
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bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 and Huh7.5 cells with PiggyBAC/ACE2 and integrase-encoding plasmids (System Biosciences, Inc.) using Lipofectamine 3000 ...
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bioRxiv - Cancer Biology 2021Quote: ... or pGreenFire1-SP1 (System Biosciences; Palo Alto, CA) and pRL-TK vector (as an inner control that contains Renilla luciferase sequences (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... miRZip anti-miR precursor constructs for miR-322 and miR-503 cluster (miRZIP-322 and miRZip-503) and pGreenPuro Scramble Hairpin control construct were purchased from System Biosciences (Palo Alto, CA), and lentiviruses were generated according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... A lentiviral plasmid containing guide RNA sequences was transfected into 293TN cells (System Biosciences) using Lipofectamine 2000 transfection reagent ...
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bioRxiv - Cell Biology 2021Quote: Complete or partial DFNA5 cDNA was cloned into PiggyBac Cumate Switch Inducible vectors (PB-Cuo-MCS-IRES-GFP-EF1-CymR-T2A-Puro or PB-Cuo-MCS-3XFlag-IRES-GFP-EF1-CymR-T2A-Puro) (System Biosciences, Palo Alto, CA). The vectors were transduced into HeLa cells using the PiggyBac transposon system (System Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... were transfected into 293TN cells (System Biosciences) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... The vectors were transduced into HeLa cells using the PiggyBac transposon system (System Biosciences, Palo Alto, CA), and the cells were selected for 2 weeks with 2 μg/ml of puromycin ...
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bioRxiv - Genomics 2021Quote: ... Exosomes were isolated via ExoQuick TC (System Biosciences, Palo Alto, CA) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Viral particles were concentrated using PEG-it (System Biosciences, Inc).