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Citations for System Biosciences :
301 - 350 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... and PB-SIN-puro include the backbone and ITR and insulator domains of the piggybac transposon vector PB-EF1α-MCS-IRES-GFP (System Biosciences PB530A-2) and contain a 5’ compound SV40 enhancer with hybrid RSV-MMSV LTR (based on SERS11 design66) ...
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bioRxiv - Neuroscience 2022Quote: NDE were isolated from total EVs of plasma using the Basic Exo-Flow Capture Kit (System Biosciences) as described (Winston et al. ...
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bioRxiv - Cell Biology 2022Quote: ... a lentivirus expression plasmid (MMIR-1a-1-PA-1 and MMIR-206-PA-1 purchased from System Biosciences) and plasmid mixture for packaging (ViraPower HiPerform Lentiviral Expression Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Stock cumate solution (System Biosciences # QM100A-1) was diluted to 5X in culture medium prior to use.
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bioRxiv - Cell Biology 2022Quote: Bare 293T cells were first co-transfected using PEI47 with the H2B-mTagBFP2 vector and Super PiggyBac Transposase-expressing vector (System Biosciences Inc. # PB210PA-1) via polyethylenimine (Sigma #408727-100mL ...
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bioRxiv - Cell Biology 2022Quote: ... and used to infect Stat3−/− MEFs in the presence of Transdux Max (System Biosciences, Palo Alto, CA, USA) reagent ...
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bioRxiv - Cell Biology 2022Quote: ... cumate (30 ug/ml; System Biosciences) was added in the media immediately after the mitotic shake-off and was washed out prior to imaging ...
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bioRxiv - Cell Biology 2022Quote: ... The pCDH-CMV-MCS-EF1-Blast was generated from pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1; System Biosciences). The annealed synthetic oligonucleotides (Table 2 ...
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bioRxiv - Developmental Biology 2022Quote: AFSC-EVs were fluorescently labelled using ExoGlow-EV labeling kit (SBI System Biosciences), using the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Constitutive lentiviral expression was performed using pCDH vector constructs (System Biosciences) and packaged using third generation packaging plasmids ...
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bioRxiv - Cancer Biology 2022Quote: ... we performed the Exo-check Antibody Array (System Biosciences, Palo Alto CA, #EXORAY210A). 30 μg of EV protein was aliquoted and immunoblots were processed and developed according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... HEK293TN cells (System Biosciences, cat. no. LV900A-1) and HeLa cells (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH ...
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bioRxiv - Cell Biology 2022Quote: ... TMEM106b and TfR DNA was amplified for insertion as an N-terminal fusion to mApple and subcloned into the third-generation lentiviral plasmid pCDH-EF1-IRES-Puro (System Biosciences). The plasmid encoding CMV-EGFP was generated by digesting sfGFP-TFR-20 (RRID ...
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bioRxiv - Cancer Biology 2022Quote: ... The cDNA was subsequently cloned into the pLVX tight puro vector using the Cold FusionTM cloning kit (System Biosciences), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Human wild-type or mutant (K4M) histone H3.1 were cloned into pCDH-EF1-MCS-IRES-RFP (System Biosciences, CD531A-2). To express sgRNAs ...
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bioRxiv - Neuroscience 2022Quote: ... dual-reporter lentiviral plasmids pLL410_EF1a-rFLuc-T2A-GFP-mPGK-Puro (LL410PA-1) and pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro (LL310PA-1) were obtained from System Bioscience. Lentiviral generation was carried out as previously described [11] ...
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bioRxiv - Cell Biology 2022Quote: ... the concentrated EVs were further isolated from the remaining medium using ExoQuick-TC (SBI System Bioscience, Palo Alto, CA, USA). The isolation was performed accordingly to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The amplified coding sequences followed by p2a-tdTomato were cloned downstream of the endothelial specific fli1a promotor into a Tol2 enabled vector using the Cold Fusion Cloning kit (System Biosciences, Cat# MC010B-1).
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bioRxiv - Bioengineering 2022Quote: Gene editing of COS-7 cells were performed by electroporation of COS-7 cells with Super PiggyBac plasmid (PB210PA-1, System Biosciences) and one of the 5 plasmids of pBv1-EF-6X ...
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bioRxiv - Genomics 2022Quote: ... shRNA templates were cloned into pGreenPuro lentivector (System Biosciences, SI505A-1). shRNA targets are ...
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bioRxiv - Cell Biology 2022Quote: ... 293-TN cells (System Biosciences, SBI) were co-transfected with the lentiviral plasmid and lentiviral helper plasmids (psPAX2 and pMD2.G ...
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bioRxiv - Molecular Biology 2022Quote: ... Colonies were stained using an Alkaline Phosphatase Assay Kit (System Biosciences) and evaluated under an Olympus CK2 microscope.
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bioRxiv - Pharmacology and Toxicology 2022Quote: We used the human iPSC lines mciPS (model no. SC301A-1; System Biosciences, Palo Alto, CA, USA), SBAD2 [75] ...
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bioRxiv - Bioengineering 2022Quote: ... 100 µL of ExoQuick-TC (System Biosciences) was added ...
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bioRxiv - Immunology 2022Quote: ... VLPs were precipitated from the clarified supernatant by the addition of PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA) at a ratio of 1:10 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK 293T cells cultured in 10-cm Petri dishes at a 50-60% confluence were co-transfected with 10 μg lentiviral plasmid expressing miR-124-1 precursor under the CMV promoter and GFP under the EF1 promoter (SBI System Biosciences) or 10 μg lentiviral plasmid expressing GFP under the CMV promoter and the packaging plasmids pVSV-G (3.5 μg) ...
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bioRxiv - Biophysics 2022Quote: ... wildtype U-2 OS cells (ATCC #HTB-96) were transfected with 1000 μg of PiggyBac-HaloTag GR and 250 μg of Super PiggyBac Transposase (System BioSciences #PB200A-1) and 3 μL of TransIt LT1 (Mirus #MIR2305) ...
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bioRxiv - Biophysics 2022Quote: ... PB-CMV-MCS-EF1α-Puro (System Bioscience #PB510B-1) was linearized using EcoRI and BamHI ...
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bioRxiv - Cell Biology 2022Quote: ... Culture medium containing the shed virus was collected at 72 hours and incubated with PEG-it virus precipitation solution (System Biosciences) at 4°C for 48 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... using PureFection (SBI System Biosciences) following the manufactures protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 293-TN cells (System Biosciences, SBI) at 70-80% confluency were co-transfected with the lentiviral plasmid and lentiviral helper plasmids (psPAX2 ...
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bioRxiv - Neuroscience 2022Quote: shRNAs targeting human HNRNPR were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing EGFP according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and Exo-Check™ exosome antibody array (EXORAY200B-4) were purchased from SBI (System Biosciences). miRCURY LNA™ RT kit ...
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bioRxiv - Developmental Biology 2022Quote: ... 300 ng of Super PiggyBac Transposase (SBI System Biosciences), 700 ng of PiggyBac-hgRNA L21 library ([9]) ...
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bioRxiv - Immunology 2022Quote: ORF7a or ORF7b coding sequences (codon-optimized for mammalian expression) were cloned into pLVX-EF1α-IRES-Puro Cloning and Expression Lentivector (System Biosciences) to generate pseudotyped lentiviral particles encoding the ORF7a or ORF7b accessory proteins of SARS-CoV-2 (Wuhan-Hu-1 isolate ...
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bioRxiv - Immunology 2022Quote: ... and inserted into pCDH expression vector (System Biosciences, Mountain View, CA). To generate chDNAM-1-expressing vectors ...
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bioRxiv - Cancer Biology 2022Quote: ... pGreenpuro shRNA cloning and expression lentivector was purchased from System Bioscience (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... a plasmid containing the truncated ATR cDNA (containing the first 779 codons only) and a Kanamycin resistance marker was obtained (System Biosciences, SBI, Palo Alto, CA). This plasmid was built into SBI’s FC500A-1 vector that contains attB sites which allow the target sequence to be stably integrated into mammalian genomes in low copy number when co-transfected with ϕC31 integrase ...
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bioRxiv - Neuroscience 2022Quote: ... The open reading frame from of murine XPR1 was tagged with a sequence for the HA-epitope and cloned into PiggyBac expression vector PB510B-1 (System Biosciences) with XbaI and NotI ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lentiviral particles were precipitated by PEG-it (System Biosciences) as described by the manufacturer and were resuspended in sterile 1xPBS and frozen in aliquots at −80°C ...
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bioRxiv - Neuroscience 2022Quote: ... and after three days harvested and concentrated the Virus with the AAVanced™ Concentration Reagent (System Biosciences).
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from the EVs using the Exoquick RNA column purification kit (EQ808A-1, System Biosciences, Palo Alto, CA, USA). Purified RNA was quantified using a Bioanalyzer and the Agilent RNA 6000 Pico kit (5067-1513 ...
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bioRxiv - Molecular Biology 2022Quote: ... EVs were isolated using ExoQuick-TC (EXOTC50A-1, System Biosciences, Palo Alto, CA, USA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... supernatant containing lentiviral particles was collected and concentrated with PEGit virus precipitation solution according to manufacturer’s protocol (System Biosciences). CSCs were plated on Geltrex ...
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bioRxiv - Developmental Biology 2022Quote: Lentivirus was produced in 293TN cells (System Bioscience, LV900A-1) expanded in DMEM with 10% FBS as previously described (Beronja et al. ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids used to produce GFP-lentiviruses were the lentivector backbone pCDH-EF1α-GFP (System Biosciences), the packaging plasmid psPAXII (Addgene) ...
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bioRxiv - Cell Biology 2022Quote: ... Exosomes were labeled with ExoGlow™-Vivo EV Labeling Kit (System Biosciences, Palo Alto, CA). Labeled exosomes or PBS containing exosomes were injected via tail vein into 17-18-month-old C57BL/6 mice ...
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bioRxiv - Cancer Biology 2022Quote: ... pGreenpuro shRNA cloning and expression lentivector was purchased from System Bioscience (Cat. No. SI505A-1, System Biosciences, LLC, CA). Primers for shRNA are shown in S1 Table ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was collected at 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Solution (System Biosciences, LV810A-1). To transduce ...
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bioRxiv - Developmental Biology 2022Quote: ... freshly dissociated brain cells were first transduced with lentivirus delivering human SNIP1 transgene (System Biosciences CD823A-1) in ultra-low attachment 6-well plates ...