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Citations for System Biosciences :
251 - 300 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Cancer Biology 2023Quote: EVs were isolated from 2.5 mL of 48 h conditioned media from tissue slice explants using ExoQuick-TC (System Biosciences, Palo Alto, CA, USA) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... EVs were isolated from 500 µL fresh serum using the ExoQuick protocol (System Biosciences, Palo Alto, CA, USA). Remaining serum aliquots were snap-frozen and stored at -80°C.
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bioRxiv - Cell Biology 2023Quote: ... and 0.2 µg transposase expression vector (System Biosciences) were used ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with 1500ng of HaloTag-NLS or HaloTag-H2b + 1000ng of piggyBac Transposase (System Biosciences, PB210PA-1) using Lipofectamine2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... designed by CRISPRdirect,101 were cloned into linearized CAG-T7-hspCas9-T2A-RFP-H1-gRNA SmartNuclease vector (CAS721R-1; System Biosciences).
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bioRxiv - Bioengineering 2023Quote: ... and anti-rabbit HRP-linked secondary antibody (EXOAB-KIT-1) were purchased from System Biosciences (CA, USA). Anti-beta-actin antibody (sc-47778) ...
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bioRxiv - Cell Biology 2023Quote: ... into a customized Gateway-enabled pCDH vector (System Biosciences) and expressed under control of a EF1α promoter ...
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bioRxiv - Developmental Biology 2023Quote: ... or goat anti-rabbit (1:20,000; EXOAB-KIT-1; System Biosciences, Palo Alto, CA, USA). Protein signals were detected using the SuperSignal West Femto Chemiluminescent kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: EV pellets were diluted and 45 µL was examined with the ExoELISA-ULTRA CD63 Kit according to the manufacturer’s instructions (System Biosciences, Palo Alto, CA, USA). The sample absorbance minus the blank wells was used to determine concentrations based on the standard curve included in the kit.
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4°C with 1:1000 primary antibody (CD63 and CD81, rabbit anti-human, EXOAB-CD63A-1/ EXOAB-CD81A-1, System Biosciences, Palo Alto, CA, USA) in TBST ...
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bioRxiv - Genomics 2023Quote: ... a HCT116 dCas9-KRAB-MeCP2 stable line was first generated using the PiggyBac system (System Bioscience). The PiggyBac Donor plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: ... and PB-SIN-puro include the backbone and ITR and insulator domains of the piggybac transposon vector PB-EF1α-MCS-IRES-GFP (System Biosciences PB530A-2) and contain a 5’ compound SV40 enhancer with hybrid RSV-MMSV LTR (based on SERS11 design66) ...
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bioRxiv - Cancer Biology 2023Quote: ... PC and MCT4 expression by RNA interference were created based on pGreenPuro lentivector (System Biosciences, LLC). The shRNA template was made by annealing appropriate oligonucleotides to each other (Extended Data Fig ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase reporter assays were performed by co-transfection of miR-423-5p or miR-Ctrl construct (System Biosciences) with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR ...
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bioRxiv - Molecular Biology 2023Quote: ... and miRZip423-5p (anti-miR-423-5p) were purchased from System Biosciences Inc (Palo Alto ...
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bioRxiv - Microbiology 2023Quote: ... The resulting DNA was cloned into the Piggybac vector (System Biosciences, Palo Alto, CA, USA) containing a selective marker for puromycin drug resistance ...
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bioRxiv - Physiology 2023Quote: ... and transfected with CD81-GFP (System Biosciences # CYTO124-PA-1) and mPlum-mito-3 (Addgene plasmid #55988 ...
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bioRxiv - Zoology 2023Quote: ... EVs were isolated from the resulting supernatant using the ExoQuick Exosome Isolation kit from System Biosciences following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... CD9+/CD63+/CD81+ iEVs were separated from bulk iEVs with Exo-Flow streptavidin magnetic microbeads (System Biosciences) coupled with biotinylated human anti-mouse CD63 (Miltenyi 130-125-989) ...
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bioRxiv - Bioengineering 2023Quote: EV RNA was isolated with a SeraMir Exosome RNA Column Purification Kit (System Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The remaining half of captured iEVs were eluted from microbeads with Exosome Elution Buffer (System Biosciences), washed with DPBS ...
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bioRxiv - Biochemistry 2023Quote: ... was amplified from human cDNA using PCR primers with XbaI and BamHI overhangs and cloned into XbaI/BamHI digested pCDH-EF1-MCS-Blasticidin (System Biosciences). Standard mutagenesis was used to generate the S251A ...
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bioRxiv - Genetics 2023Quote: ... previously created by substituting the copepod GFP from the pB530A plasmid (System Biosciences) with the Puromycin resistance gene ...
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bioRxiv - Bioengineering 2023Quote: ... Half of iEVs captured by the beads were stained with Exo-FITC Universal Exosome Stain (System Biosciences) and analyzed with a BD FACSCanto™ II Flow Cytometry System ...
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bioRxiv - Bioengineering 2023Quote: Genes-of-interest such as SB-CAR and SB100X constructs were firstly cloned into a parental plasmid (System Biosciences). Then MC DNA was produced and purified by using an MC-EsayTM Kit (System Biosciences) ...
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bioRxiv - Bioengineering 2023Quote: ... Then MC DNA was produced and purified by using an MC-EsayTM Kit (System Biosciences), without the optional dNTP removal step.
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bioRxiv - Developmental Biology 2023Quote: Culture and differentiation of hiPSCs (SC101A, System Biosciences) were performed as previously described ...
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bioRxiv - Cancer Biology 2023Quote: ... HA1_BsaI_rev 5’-TAT AGA GAC CGA TGG ATA AAA GCC CAA ATT C-3’ and cloned into the multiple cloning site 1 (MCS1) of the vector MCS1-EF1α-GFP-T2A-Puro-pA-MCS2-PGK-hsvTK (HR700, System Biosciences) using BsrGI (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with the tet-L1/GLucAI donor plasmid (pBH201) and the Super PiggyBac Transposase Expression Vector (System Biosciences) at a 5:1 plasmid ratio ...
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bioRxiv - Developmental Biology 2023Quote: ... 2000 ng of the PiggyBac vector plasmid and 500 ng of the PiggyBac transposase plasmid (System Biosciences, PB210PA-1) were mixed in 125 μL Opti-MEM (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: All constructs were cloned into a PiggyBac plasmid (System Biosciences). Linear DNA fragments were amplified via PCR using CloneAmp HiFi PCR premix (Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... Constitutive lentiviral expression was performed using pCDH vector constructs (System Biosciences) and packaged using third-generation packaging plasmids ...
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bioRxiv - Bioengineering 2023Quote: ... and the pPACKH1 HIV Lentivector Packaging Kit (System Biosciences) was used to produce lentivirus particles ...
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Bioengineering 2023Quote: ... LVVs were stained with ExoGlow (System Biosciences), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... samples were purified using the CD9 Exo-Flow Capture Kit (System Biosciences) using the manufacturer-provided protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... a PEO1-OR-dCas9-KRAB-MeCP2 stable line was first generated using the PiggyBac system (System Bioscience). The PiggyBac Donor plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviruses containing the luciferase expression cassette were produced in 293 TN cells (System Bioscience, catalog number: LV900A-1) by transient transfection with the PSMPUW-Hygro lentiviral vector carrying the luciferase gene and the ViraSafe™ Lentiviral Packaging System (Cell Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... E0771 and RM-1 cells were transduced with lentiviral vectors carrying the luciferase expression cassette with TransDux MAXTM (System Bioscience, catalog number: LV860A-1). Luciferase-labeled E0771 and RM-1 cells were then selected in the presence of 300 µg/mL hygromycin ...
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bioRxiv - Molecular Biology 2023Quote: ... the SpyCas9-ABE-tracrRNA PiggyBac transposon plasmid and PiggyBac transposase expression plasmid (System Biosciences PB210PA-1) were mixed in a 2:1 molar ratio and transfected into the HEK293T-dGFP reporter cell line using Lipofectamine 3000 (ThermoFisher L3000015) ...
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bioRxiv - Microbiology 2023Quote: MVs were isolated with the ExoQuickTC reagent (EQPL10TC; System Bioscience) as described elsewhere20 ...
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bioRxiv - Cell Biology 2023Quote: ... TransDux MAX Lentivirus Transduction Enhancer (System Biosciences LV860A-1), and 10 uM Y-27632 and plated as monolayers on Collagen I (Advanced Biomatrix 5010) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25ml of cold PEG-it Virus Precipitation Solution (LV810A-1-SBI, System Biosciences) was added per 1ml of lentiviral-containing supernatant and kept cool at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: EVs from biological fluids were isolated using ExoQuick exosome precipitation solution (System Biosciences, SBI-EXOQ5A-1). Plasma and serum samples were centrifuged at 3,000 × g for 15 min to remove cells and cell debris ...
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bioRxiv - Cancer Biology 2023Quote: ... along with two housekeeping genes (Supplementary Table 1; CAS9-PR-1 from System Biosciences). The Kcnn4 and Cas9 mRNA levels for each sample were measured in technical duplicates for each primer set ...
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bioRxiv - Molecular Biology 2023Quote: ... The culture medium was collected 72 h after transfection and concentrated using AAVanced Concentration Reagent (System Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... ni piggyBac transposase (PB200PA-1; System Biosciences) (2) ...
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bioRxiv - Molecular Biology 2023Quote: ... using the AAVS1 Safe Harbor Targeting System (GE622A-1-SBI, System Biosciences). In the first round of genome editing ...
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bioRxiv - Molecular Biology 2023Quote: ... filtered using 45 um filters and concentrated 100-fold using PEG-it Virus Precipitation Solution (System Biosciences).