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Citations for System Biosciences :
601 - 650 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Exosomes were labeled with TexRed-siRNA (SBI System Bioscience) as per manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... using a 5’ primer carrying NsiI and a 3’ primer carrying P2A-NsiI and ligated into the NsiI digested pCDH-SFFV-GLuc-T2A-mCherry vector downstream of the T2A peptide (Figure S2a) (pCDH-vector, System Bioscience). For inducible knockdown of target genes ...
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bioRxiv - Cancer Biology 2020Quote: Tissues were stained with antibody for anti-CD63 (System Bioscience, Mountain View, CA, USA). The secondary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-CD63 (System Bioscience, Mountain View, CA, USA), anti-AIP1/Alix (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: Exosomes were purified from cultured medium supplemented with exosome-depleted FBS using the exosome purification kit following manufacturer’s instructions (ExoQuick™, System Bioscience, Mountain View, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... cDNA sequences were cloned into the PB-CMV-MCS-EF1α-Puro vector (PB510B-1, SBI System Biosciences) where the CMV promoter was replaced by a PGK promoter ...
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bioRxiv - Neuroscience 2020Quote: ... Harvested lentivirus was concentrated using PEG-it (™) virus precipitation solution (System Biosciences, CA) and processed for titration.
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bioRxiv - Microbiology 2020Quote: ... DMEM exosome-free media was prepared using exosome-depleted FBS from System Biosciences (Palo Alto, CA, USA).
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bioRxiv - Microbiology 2020Quote: EV isolation from mouse serum was performed using ExoQuick™ solution (System Biosciences, Palo Alto, CA, USA). Following serum collection ...
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bioRxiv - Cancer Biology 2020Quote: ... EVs were isolated using ExoQuick-TC (System Biosciences) according to manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 and MDA-MB-231-Luc+ cells were transduced with lentivirus vector expressing a GFP or RFP tag fused to the membrane-associated tetraspanin CD63 (System Biosciences, Palo Alto, CA). Breast cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2020Quote: ... UCHL1 knockdown was also carried out by transducing HGSOC cells with control or UCHL1 shRNA lentiviral virus particles (Santacruz Biotechnology; cat# sc-108080 and sc-42304-V) using TransDux™ MAX (System Biosciences; cat#LV860A-1). Cells were treated with Carfilzomib (cat# S2853 ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293TN cells were purchased from System Biosciences. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... EV depleted FCS was either commercially obtained (System Biosciences Catalog no. EXO-FBS-250A-1) or prepared by ultracentrifugation of the FCS used at 120,000 x g for 5 hours ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... based on a pCDH derived vector (System Biosciences) so that mtagBFP ...
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bioRxiv - Cell Biology 2020Quote: ... P2A-mScarlet (gift from A. Beisaw) cloned with Cold Fusion (System Biosciences). Tg(ins:mCardinal ...
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bioRxiv - Biophysics 2020Quote: ... into the lentiviral vector CD813A (System Biosciences) encoding the 5’ untranslated region of human β-globin (HBB 5’-UTR).
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... It was thereafter inserted into pCDH-CMV-MCS-EF1-puro vector (System Biosciences, Palo Alto, CA), resulting in vector mNeonGreen-puro ...
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bioRxiv - Cell Biology 2020Quote: ... or the SNAP-GalT-GFP construct29 encoded in a pCDH-EF1-MCS-IRES-Puro vector (SBI System Biosciences), or short hairpin RNA (shRNA ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was blocked in freshly prepared 5% BSA diluted in TBS before being immunoblotted with specific primary antibodies (CD81; #EXOAB-CD81A-1 System Biosciences, GM130 ...
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bioRxiv - Microbiology 2020Quote: ... hFhl1 gene encoding FHL1A isoform fused with GFP was cloned into modified PiggyBac plasmid (System Bioscience, Inc) under control of the CMV promoter ...
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bioRxiv - Cancer Biology 2020Quote: ... Double-stranded oligonucleotides were cloned into the linearized SmartNuclease vector (EF1-T7-hspCas9-T2A-RFP-H1-gRNA with red fluorescent protein (RFP, System Biosciences) and IFNLR1-guide RNA (IFNLR1-gRNA ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells (ATCC® ACS-4500™) were transiently transfected with hACE2 fusion BFP encoding PB510 plasmid using PiggyBac Transposon System (System Biosciences, PB210PA-1), followed by addition of 2 μg/mL puromycin 6 h post-transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... we replaced the KRAB and dCas9 cassettes from the pHR-SFFV-KRAB-dCas9-mCherry vector with the HA-tag-MEOX1 cassette using Cold-fusion cloning kit (SBI System BioSciences, Palo Alto, CA, USA) following the instruction provided by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... miR-Zip control and miR-Zip-200c plasmids were purchased from System Biosciences.
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bioRxiv - Neuroscience 2020Quote: ... Lentivirus packaging was provided by System Biosciences (Palo Alto, CA). Titers of VSV-G pseudotyped viral particles were ~107 IFUS/mL.
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bioRxiv - Immunology 2020Quote: ... Supernatant containing viral particles was collected and concentrated using PEG-IT (System Biosciences) followed by centrifugation at 2600 rpm ...
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bioRxiv - Immunology 2020Quote: ... NK92 CD56-KO cells were transduced with pBabe-puro-NCAM mApple viral particles in the presence of MAX Enhancer and TransDux (System Biosciences). Within 48 hours cells were sorted for CD56 and mApple and cells were expanded and maintained under antibiotic selection with regular phenotypic validation.
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bioRxiv - Biochemistry 2020Quote: ... and also cloned into pCDH-MSCV-MCS-EF1α-GFP+Puro lentiviral vector (System Biosciences, Cat# CD713B-1, Palo Alto, CA) for constitutive expression ...
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bioRxiv - Microbiology 2020Quote: The 3’UTR reporter plasmids were co-transfected into HEK293T cells using PEI max together with the indicated pCDH-EF1-MCS expressing plasmids (System Biosciences) containing a cloned pre-miRNA gene of interest ...
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bioRxiv - Cell Biology 2020Quote: The transfection of FAPs with pCT-CD63-GFP plasmid (#CYTO120-PA-1, System Biosciences) and with a GFP-control vector (mock ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus was concentrated by adding 2500 μl ice-cold PEG-IT (System Biosciences) to 10 ml harvested supernatants and incubating overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cancer Research UK) into the lentiviral vectors pCDH-EF1α-MCS-IRES-Neo and pCDH-EF1α-MCS-IRES-Puro (System Biosciences) cut by EcoRI(blunt)-BamHI ...
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bioRxiv - Molecular Biology 2020Quote: ... A Myc-tagged MGMT fragment containing 5’-XbaI extremities and 3’-BamHI extremities was generated by PCR amplification and restriction enzyme digestion and cloned into the lentiviral vector pCDH-EF1α-MCS-IRES-Neo and pCDH-EF1α-MCS-IRES-Puro (System Biosciences) pre-digested with the same restriction enzymes ...
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bioRxiv - Genomics 2020Quote: ... and concentrated using PEG-it (SBI System Biosciences) according to the manufacturer’s recommendations.
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bioRxiv - Physiology 2020Quote: ... Supernatants were collected and purified using PEG-it (System Biosciences). Concentrated lentivirus was stored at −80 °C for transduction of primary human cells.
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PB510B-based sgRNA expression vector and PB533-dCas9-EGFP were co-transfected with a transposase expression vector PB200PA-1 (System BioSciences) into MC12 cells ...
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bioRxiv - Developmental Biology 2020Quote: ... dCas9-EGFP was also subcloned into another piggyback vector PB533-A2 (System BioSciences). The resulting PB510B-based sgRNA expression vector and PB533-dCas9-EGFP were co-transfected with a transposase expression vector PB200PA-1 (System BioSciences ...
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bioRxiv - Developmental Biology 2020Quote: ... 7 that exhibited distinct spots by transient expression (Supplementary table 1) were subcloned into a piggybac-vector PB510B-1 (System BioSciences) to establish stable lines ...
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bioRxiv - Developmental Biology 2020Quote: ... scFv-sfGFP was cloned into PB533A (System Biosciences) and transfected with the transposase expression vector (PB200PA-1 ...
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bioRxiv - Cell Biology 2020Quote: ... Culture medium was collected 72 hours post transfection and concentrated with AAVanced Concentration Reagent (#AAV100A-1, System Biosciences).
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bioRxiv - Synthetic Biology 2020Quote: ... All constructs were cloned into the piggyBac plasmids (System Biosciences Inc.) driven by a synthetic version of human EF1A promoter.
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bioRxiv - Synthetic Biology 2020Quote: ... Each of the individual rTetR-effector plasmids was randomly integrated into this reporter line by co-transfecting 250 ng Super PiggyBac Transposase expression vector (System Biosciences #PB200PA-1) and 750 ng of rTetR-effector donor vector ...
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bioRxiv - Synthetic Biology 2020Quote: The plasmids containing the rTetR-effector fusions were cloned into the PB-CMV-MCS-EF1α-Puro PiggyBac vector backbone (System Biosciences #PB510B-1), which was further modified via Gibson Assembly with the following components ...
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bioRxiv - Microbiology 2020Quote: ... Fusion was performed with the Cold Fusion Cloning Kit (SBI System Biosciences, Cat. #MC010B-1).
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bioRxiv - Molecular Biology 2020Quote: The pCDH-EF1-Puro plasmid was obtained from System Biosciences. The pCDH-DJ-1 WT and mutants were constructed using the primers listed in table S2 ...
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bioRxiv - Physiology 2020Quote: EVs were isolated from SC culture media using ExoQuick TC (System Bioscience, Palo Alto, USA), following the manufacturer’s instructions and resuspended in 1X PBS for long term storage at −80°C ...
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bioRxiv - Neuroscience 2020Quote: Primary DRG neuronal cultures isolated as described above were transduced with pGreenFire1-LXRE in LXRα Lentivector (System Biosciences, California) followed by respective group treatment 48hrs after transduction ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: Constructs that express V5-tagged and MS2V5-tagged proteins were stably integrated into the cells using the PiggyBac (PB) Transposon system and the cumate-inducible PB-CuO-MCS-IRES-GFP-EF1-CymR-Puro vector (System Biosciences). 2.5×105 cells were seeded 24 h before transfection in 6-wells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and ligated into the CAGs-MCS-Enhanced Episomal Vector (EEV) (System Biosciences, EEV600A-1) linearized with AgeI and KpnI ...