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Citations for System Biosciences :
201 - 250 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: The T2A sequence within the pCDH-EF1α-MCS-T2A-Puro plasmid (System Biosciences) was replaced with the IRES sequence from the pMXs-IRES-Puro (Cell Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK 293TN (LV900A-1) cells were purchased from System Biosciences (Palo Alto, USA). Mouse mesothelial cells (MCs ...
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bioRxiv - Immunology 2023Quote: ... with 1x Cumate solution (System Biosciences) to induce protein expression ...
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bioRxiv - Immunology 2023Quote: ... Inserts were introduced into the pCDH-CuO-MCS-EF1a-CymR-T2A-Puro SparQ™ All-in-one Cloning and Expression Lentivector (QM800A-1, System Biosciences). The correct sequence of the gene of interest (GOI ...
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bioRxiv - Developmental Biology 2023Quote: ... and 420 ng PBase ‘helper’ plasmid (System Biosciences). After preparation ...
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Immunology 2023Quote: ... Constitutive lentiviral expression was performed using pCDH vector constructs (System Biosciences) and packaged using third-generation packaging plasmids ...
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bioRxiv - Synthetic Biology 2023Quote: The basal cell line expressing rTetR-DNMT3B (CD and CI version) is constructed by transfection and stable integration via the PiggyBAC system (System Biosciences), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... ExoQuick-TC ULTRA (for cell culture medium) (System Biosciences) was used according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... The in vivo T cell activation reporter was constructed by cloning a sequence containing firefly luciferase into the pGreenFire1-NF-κB lentiviral vector (System Biosciences) under the NF-κB responsive promoter ...
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bioRxiv - Genomics 2023Quote: Clones A and E were transfected with a Piggybac-PEmax plasmid with the Piggybac transposase (System Biosciences, Cat. No. PB210PA-1) at a 10:1 molar ratio using the transIT-LT1 transfection reagent (Mirius Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... with Super PiggyBac Transposase (System Biosciences) and the PiggyBac transposon vector with human GATA6-2A-EGFP under control of the tetracycline responsive element promoter ...
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bioRxiv - Developmental Biology 2023Quote: ... with Super PiggyBac Transposase (System Biosciences) and the PiggyBac transposon vector with hGATA6-2A-EGFP under control of the tetracycline responsive element promoter ...
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bioRxiv - Biochemistry 2023Quote: The PiggyBac-cumate-ERGIC-53-PA tag plasmid along with the Super PiggyBac Transposase Expression Vector (System Bioscience) was transfected to generate a stable HEK293T cell line with cumate inducible ERGIC-53 expression ...
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bioRxiv - Bioengineering 2023Quote: Western blotting was performed using ExoA Antibody Kit according to the manufacturer’s protocol (System Biosciences, USA). Immunoblots were cut prior to primary antibody hybridization ...
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bioRxiv - Neuroscience 2023Quote: ... These sequences were inserted into the U6 promoter-driven lentiviral vector using the Lentiviral CRISPR/Cas9 System (CASLV511PA-G System Biosciences, Palo Alto, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... or luciferase used the same packaging and envelope plasmids as described previously with the respective 3rd generation plasmids: pCDH-MSCV-MCS-EF1-GFP-T2A-Puro (System Biosciences, CD713B-1), pLenti-mCherry (derived from pLenti-III-EF1a ...
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bioRxiv - Immunology 2023Quote: ... pCDHv6_PTPRZ1 (derived from pCDH-MSCV-MCS-EF1-GFP-T2A-Puro, System Biosciences, CD713B-1), with packaging plasmids pMDLg/pRRE (RRID:Addgene_12251) ...
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bioRxiv - Biochemistry 2023Quote: ... The spike DNA sequence was cloned into pMRNAXP mRNA Synthesis Vector (System Biosciences) that contains T7 promoter ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... were encapsulated in PureFection nanoparticles (System Biosciences, Palo Alto, CA) and added drop-by-drop to the culturing cells ...
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bioRxiv - Cell Biology 2023Quote: ... the media was supplemented with 1X cumate (System Biosciences QM159A-1) to induce expression of the rescue constructs ...
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bioRxiv - Cell Biology 2023Quote: ... 1.8 µg PB533-or PB510-based PiggyBac plasmid (System Biosciences) and 0.2 µg transposase expression vector (System Biosciences ...
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bioRxiv - Genetics 2023Quote: The PB EGFP donor vector (PB-CMV-MCS-EF1ɑ-GFP, #PB511B-1) was purchased from System Biosciences (Palo Alto, CA). The PiggyBac transposase (PBase ...
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bioRxiv - Developmental Biology 2023Quote: To generate ESC lines carrying Dox-inducible dCas9, plasmid pT7077 (Hazelbaker et al., 2020) (addgene 137879) was co-transfected with Super piggyBac Transposase expression vector (System Biosciences) using Lipofectamine™ 3000 Transfection Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... pPN4586× -MT2_Mm was co-transfected with Super piggyBac Transposase expression vector (System Biosciences) into an ESC line with Dox-inducible dCas9 ...
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bioRxiv - Genomics 2023Quote: ... The collected virus was concentrated 100-fold after precipitation with PEG-itTM Virus Precipitation Solution (System BioSciences LV810A-1) for 3 days ...
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bioRxiv - Immunology 2023Quote: ... into pCDH533-IRES-Neo (System Bioscience). For pCDHTRE-K6a-mCherry ...
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bioRxiv - Genetics 2023Quote: ... cells were transfected with 0.72 μg of piggyBac transposase plasmid (System Biosciences PB210PA-1) and 1.78 μg of pCX4.1 plasmid using Lipofectamine 3000 transfection reagent ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was transferred to a sterile vessel and added Exo Quick-TC (System Biosciences, Palo Alto, CA) (supernatant ...
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bioRxiv - Molecular Biology 2023Quote: ... U2-OS cells (UC Berkeley BDS Cell Culture Facility) cultured in DMEM (1 g/L glucose, 10% FBS) were transfected with a modified PiggyBac Transposase plasmid (System Biosciences) and a PGK.mNeonGreen-LacI.NLS.IRES.Puro integration plasmid using Fugene6 transfection reagent ...
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bioRxiv - Molecular Biology 2023Quote: ... and the viral supernatants were passed through 0.45-μm filters and concentrated using PEGit Virus Precipitation Solution (System Biosciences). The lentiviral vectors were resuspended in Opti-MEM (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... and pVSVG (System Biosciences) into HEK293TN cells ...
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bioRxiv - Genomics 2023Quote: ... Fragments were resuspended at a final concentration of 100ng/ul in EB buffer and then cloned into a PB-CMV-MCS-EF1α-Puro PiggyBac cDNA Cloning and Expression Vector (PB vector hereafter, System Biosciences #PB510B-1). Following ligation ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: To allow propagation of the piggyBAC transposase from System Biosciences on ampicillin plates ...
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bioRxiv - Microbiology 2023Quote: ... The EVs were precipitated using ExoQuick-TC reagent (System Biosciences) following the manufacturer’s instructions and depleted of serum proteins using 100 kDa spin columns ...
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bioRxiv - Cancer Biology 2023Quote: ... The transfection was completed following purefection transfection reagent protocol (System Biosciences, LV750A). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The viruses from each time point were pooled and concentrated using PEG-it virus precipitation solution (System Biosciences, LV810A) according to manufacture’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The pSIH1-H1-siLuc-copGFP plasmid (System Bioscience, LV601PB) was used as a positive transduction control.
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bioRxiv - Cancer Biology 2023Quote: ... MCF10A cells were seeded to achieve 50% to 70% confluency and the following day the virus particles were added to the dish along with TransDux virus transduction reagent (System Biosciences, LV850A). The media was changed after 4 days and antibiotic selection was performed using a puromycin concentration of 0.85µg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... OMVs in the total supernatant fraction were extracted using the ExoBacteria™ OMV Isolation Kit (System Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293TN cells were obtained from System Biosciences and cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... omentum tissues were minced with sterile razor blades and approximately 0.5 g of minced tissue was cultured per 100 mm tissue culture dish in 10 mL DMEM/F12 media supplemented with 1% exosome-depleted FBS (EXO-FBS-250A-1, System Biosciences, Palo Alto, CA). The following day ...
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bioRxiv - Bioengineering 2023Quote: Stable HeLa-Fucci cell line was generated using the Super PiggyBac Transposase Expression Vector system (System Biosciences, PB210PA-1) together with tFucci(CA)5 plasmid29 (Addgene #153521) ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience) vector ...
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bioRxiv - Cancer Biology 2023Quote: ... A second ESR1-DAB2 plasmid in a pCDH-CMV-MCS-ER1α-Puro (System Biosciences #CD510B-1) was received as a kind gift from Dr ...
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bioRxiv - Cell Biology 2023Quote: ... PiggyBac vector was purchased from System Biosciences (PB210PA-1; Palo Alto, CA). For transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the PCDF1-MCS2-EF1-Puro expression vector (System Biosciences, CD110B-1). We performed a restriction digest and gel extraction as above to the plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to a tube along with purefection reagent and pPackF1 lentiviral packaging kit plasmids (System Biosciences, # LV100A-1) and either PCF1 (empty vector control) ...
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bioRxiv - Bioengineering 2023Quote: ... The amplified regions were assembled into the PiggyBac transposon backbone (System Biosciences) between a TRE3G promoter (Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... The constructed donor plasmid was co-transfected with PiggyBac transposon vector (PB210PA, System Biosciences) into HEI-OC1 cells ...