Labshake search
Citations for System Biosciences :
551 - 600 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto ...
-
bioRxiv - Cell Biology 2021Quote: The PiggyBac Transposon system (System Biosciences) was used to generate stable cell lines expressing either GFP-tagged Golgi enzyme chimeric reporters under a cumate-inducible promoter or GOLPH3L and GOLPH3 under a CMV promoter ...
-
bioRxiv - Cell Biology 2021Quote: The backbone of the original plasmid pCDH-CMV-MCS-EF1-copGFP-T2A-Puro (System Biosciences, Palo Alto, USA) was amplified and linearized via PCR using the high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio ...
-
bioRxiv - Cell Biology 2021Quote: ... The vector was modified from the PiggyBac vector PBQM812A-1 (System Biosciences) in which the IRES and downstream GFP were removed (John Shin) ...
-
bioRxiv - Cell Biology 2021Quote: ... stable U2OS cell lines expressing Golgi enzyme chimeric reporters under a cumate-inducible promoter were maintained in the presence of 60 μg/ml cumate (System Biosciences). Cells were passaged every 3-4 days in which they were treated with trypsin at 37°C for 2 minutes ...
-
bioRxiv - Genomics 2021Quote: ... Filtered supernatant was concentrated using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... we seeded cells at ∼30% confluency in exosome-depleted medium that was obtained either by ultracentrifugation of DMEM plus 10% FBS or DMEM supplemented with 10% exosome-depleted FBS (System Biosciences, Palo Alto, CA) in 150 mm CellBIND tissue culture dishes (Corning ...
-
bioRxiv - Molecular Biology 2021Quote: ... and inserted into PB-CuO-MCS-BGH-EF1-CymR-Puro (modified from System Biosciences), together with an N-terminal FLAG-emGFP-tag via NheI and NotI (both New England Biolabs ...
-
bioRxiv - Microbiology 2020Quote: ... two modified PiggyBac (PB) transposon vectors (MIHIPsMie and EIRBsMie) were constructed based on PB-CMV-MCS-EF1α-RedPuro (System Biosciences, PB514B2). The fragment of CMV-MCS-EF1α-RedPuro on this vector was removed by SfiI/ApaI digestion ...
-
bioRxiv - Biophysics 2020Quote: ... Piggybac plasmid PB513Bm2 was made by removing copGFP from PB513B-1 (System Biosciences) by PCR-based mutagenesis ...
-
bioRxiv - Systems Biology 2020Quote: ... GFP gene was amplified from pGreenPuro shRNA Cloning and Expression Lentivector (CMV; System biosciences, SI505A-1) and sub-cloned into ITR-CAG-DEST-IRES-Neomycin-ITR plasmid (generous gift from Marc Supprian Schmidt) ...
-
bioRxiv - Microbiology 2020Quote: ... for 2 h at 37°C and plasmid-safe DNase (System Bioscience) overnight at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... ZYCY10P3S2T competent bacteria (System Bioscience) were then transformed with the pMC-HBV (WT ...
-
bioRxiv - Cell Biology 2020Quote: ... complementary primers were hybridized and cloned into the BamHI and EcoRI sites of the lentiviral pSIH-H1-copGFP-shRNA vector (System Biosciences) according to the manufacturer’s instructions to generate pSIH-T1 and pSIH-T2 plamsids for constitutive knockdown ...
-
bioRxiv - Cell Biology 2020Quote: ... along with PiggyBac Transposase expression plasmid (System Biosciences) and selected with 500 μg/ml G418 (Invitrogen) ...
-
bioRxiv - Cell Biology 2020Quote: ... isochronic and heterochronic cultures were established with Exosome-depleted FBS Media Supplement (System Biosciences, Palo Alto, CA). Culture media collected on the 4th and 7th days were pelleted and supernatant transferred to another tube for further clarification at 2000 g for 30 min to remove residual cells and debris ...
-
bioRxiv - Cell Biology 2020Quote: ... ezrin and LOK cDNAs were subcloned into pCDH lentivector (System Biosciences). Puromycin gene in pCDH was then substituted for blasticidin using Gibson assembly ...
-
bioRxiv - Cell Biology 2020Quote: ... lentiviral transfer vectors were generated by recombination into customized pCDH (System Biosciences CD532-A) destination vectors using a Gateway LR reaction ...
-
bioRxiv - Cell Biology 2020Quote: ... or a pCDH vector (System Biosciences, CD550A-1). JNK1 (NCBI RefSeq NM_001323302.1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and was cloned into a modified CD813A vector (System Biosciences) containing the beta-globin (HBB ...
-
bioRxiv - Developmental Biology 2020Quote: ... around the sgRNA1 target site at +9bp from the start of TBX5 exon 3 was cloned by Cold Fusion (System Biosciences #MC010B) using amplicons from genomic DNA of WTC11 into a construct that was a modification of plasmid pEN114 (Nora et al. ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Viral supernatants were collected 48 hours-post transfection and passed through a 0.4 μm followed by overnight incubation with 1X PEG-IT solution (System Biosciences) at 4°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... ESC were washed twice with PBS and incubated with AP Staining kit™ (Cat # AP100R-1, System Biosciences) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a sterile 1.5 mL microtube and total EVs were sedimented by incubation with 252 μL of ExoQuick® (System Biosciences) for 1 hour at 4 °C followed by centrifugation at 1,500 g for 20 minutes at 4 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... and the membrane and intra-vesicular positive EV markers CD81 (1:500; cat. no. EXOAB-CD81A-1, System Biosciences) and alix (0.4 μg/mL ...
-
bioRxiv - Developmental Biology 2020Quote: Human SC102A-1 (System Bioscience) and chimpanzee Sandra A iPSC lines (Camp et al. ...
-
bioRxiv - Molecular Biology 2020Quote: MDEs were isolated from G1-G3 bovine and human milk samples (1.5 mL/sample) using the EQ reagent (System Biosciences: EXOQ5A-1) (13,14,38,40,44) ...
-
bioRxiv - Molecular Biology 2020Quote: ... A forward primer of unknown sequence for XMIR-NT was initially purchased from System Bioscience (Palo Alto, CA, USA), then later custom ordered from IDT after plasmid sequencing ...
-
bioRxiv - Molecular Biology 2020Quote: ... stem-loop sequence was retrieved from www.miRBase.org and used to design oligonucleotides for cloning into the XMIRXpress pre-linearized cloning lentivector (cat. no. XMIRXP-Vect, System Biosciences), according to vendor’s instructions (XMIR-c39-top ...
-
bioRxiv - Molecular Biology 2020Quote: ... exosome-depleted FBS (cat. no. EXO-FBS-50A-1, System Biosciences) was added to the cells ...
-
bioRxiv - Genomics 2020Quote: ... overnight at 37°C and cloned into a linearized pCDH-EF1alpha-MCS-BGH-PGK-GFP-T2A-Puro lentivector (CD550A-1, System Biosciences) using T4 DNA ligase (New England BioLabs ...
-
bioRxiv - Cell Biology 2020Quote: ... with the respective lamin containing or empty (Mock) vectors into HEK 293-TN cells using PureFection nanotechnology-based transfection reagent (System Biosciences). The medium was exchanged the next day and replaced by antibiotics-free DMEM containing 10% FCS ...
-
bioRxiv - Cell Biology 2020Quote: ... and Q03252 (lamin B2 short version according to (Schumacher et al., 2006)] were cloned into lentiviral PCDH-EF1-MCS1-puro vectors (System Biosciences).
-
Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... we developed the excision-only PB transposase plasmid pXPBase-GFP through multiple cloning steps modifying PB220PA-1 (System Biosciences). The ccdB gene was removed to render the plasmid generally propagable ...
-
Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We created the PB transposase plasmid pSPBase by removing the ccdB gene from PB210PA-1 (System Biosciences) to render it generally propagable ...
-
bioRxiv - Developmental Biology 2020Quote: ... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
-
bioRxiv - Developmental Biology 2020Quote: ... DLD-1 cells were co-transfected with a PiggyBac transposase vector (System Biosciences, PB210PA-1) and a PiggyBac-ABA-CRISPRi vector (25 ...
-
bioRxiv - Cancer Biology 2020Quote: The piggyBac transposon vector was constructed by inserting a random 10-bp DNA barcode sequence between the CMV promoter and the multiple cloning site of the piggyBac dual promoter vector (PB513B-1; System Biosciences). The GFP sequence in the piggyBac vector was deleted by a site-directed mutagenesis technique for the direct repeat–green fluorescent protein (DR-GFP ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and then transfected with three piggyBac vectors expressing multiple human proteins with the Neon Transfection System with the presence of piggyBac Transposase (System Biosciences, USA). Two days after ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The human proteins were assigned to 3 Piggybac vectors (System Biosciences, USA) by Golden Gate Assembly method (11 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 293TN (System Biosciences, SBI, Mountain View, CA, USA)—were maintained in DMEM containing 10% FBS and 1% antibiotics ...
-
bioRxiv - Biochemistry 2020Quote: ... and a lentiviral transfer vector CD512-EF1a-RFP (System Biosciences CD512B-1) using Lipofectamine 2000 ...
-
bioRxiv - Bioengineering 2020Quote: ... viral supernatant was concentrated using PEG-it virus precipitation solution (System Biosciences LV825A-1) according to manufacturer instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... 100 ml of LB broth together with 100 µl of 20% arabinose induction solution (System Biosciences, Palo Alto, CA, USA) and 4 ml of 1N NaOH was added to the culture and grown for 5.5 hrs at 30°C ...
-
bioRxiv - Bioengineering 2020Quote: ... coli (System Biosciences, Palo Alto, CA, USA) were transformed with the original parental plasmids (PP ...
-
bioRxiv - Biochemistry 2020Quote: ... The secreted EVs were partially purified from cultured medium by using ExoQuick-TC Exosome Precipitation Solution (20 μl/100 μl serum; System Biosciences, CA) at 4°C for overnight ...
-
bioRxiv - Biochemistry 2020Quote: ... anti-TSG101 antibody (EXOAB-TSG101-1; System Biosciences, CA; 1:500), anti-CD63 antibody (EXOAB-CD63A-1 ...
-
bioRxiv - Biochemistry 2020Quote: ... anti-CD63 antibody (EXOAB-CD63A-1; System Biosciences, CA; 1:500), anti-CD5L antibody (AF2834 ...
-
bioRxiv - Biochemistry 2020Quote: ... The serum (50-100 μl) was treated with ExoQuick Exosome Precipitation Solution (25.2 μl/100 μl serum; System Biosciences, CA) on ice for 30 min ...