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Citations for System Biosciences :
151 - 200 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... SapI sites surrounded the sORF sequences to enable cloning into the custom-modified pCDH_EF1_IRES_Puro lentiviral expression plasmid (System Biosciences, CD532A-2). Following golden gate cloning using SapI (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... was used for plasmid transfection and was cloned into pCDH-CMV-MCS-EF1-GFP lentivector (System Biosciences, Inc.). The ON-TARGET siRNA-SMART pool L-003551-00-0005 or L-003467-00-0005 was used for VIM and CAV1 knockdown (Horizon Discovery) ...
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bioRxiv - Cell Biology 2024Quote: ... RIPK1 was subsequently reintroduced with the Piggybac transposon system (PB-Cuo-MCS-IRES-GFP-EF1a-CymR-Puro Cumate inducible vector, PBQM812A-1, System Biosciences), and cells were selected with puromycin 1µg/ml for 7-10 days ...
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bioRxiv - Cell Biology 2024Quote: ... RIPK1 expression was induced with water-soluble Cumate solution (QM150A, System Biosciences) for the times indicated.
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bioRxiv - Cell Biology 2024Quote: ... Supernatant was combined with PEGit concentrator (5X) (LV810A-1, System Biosciences) overnight at 4°C and centrifuged at 1500 x g for 45 minutes at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... pseudoviral particles were generated using 293TN cells (System Biosciences, SBI) co-transfected with lentiviral packaging and envelope plasmids (psPAX and pMD2.G ...
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293FT cells were transfected with the vector and transposon plasmid (System Biosciences). Transfected Cells were incubated for 72 hours before selection with media containing 2 ug/mL Puromycin (Invivogen ant-pr-1) ...
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bioRxiv - Synthetic Biology 2023Quote: Two expression cassettes each carrying a CAG promoter driving the expression of NCas13d-GAI-T2A-BFP and GID-Cas13dC-T2A-iRFP were packed into a single PiggyBac-transposon vector plasmid (System Biosciences). HEK293FT cells were transfected with the vector and transposon plasmid (System Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... EVs were precipitated using ExoQuick-TC (System Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Microvesicles were isolated using ExoQuick-TC kit (System Biosciences, Palo Alto, USA) accordingly to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Stable cell lines were generated by cotransfection with the Super PiggyBac Transposase Expression Vector (cat# PB210PA-1, System Biosciences) followed by selection via addition of 5 μg/mL puromycin (cat# ant-pr-1 ...
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bioRxiv - Biochemistry 2023Quote: The PiggyBac Single Promotor vector (cat# PB531A-1, System Biosciences) was modified in house to replace the gene encoding the red fluorescent protein (RFP ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Cold Fusion kit (System Biosciences Inc) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... were seeded and transfected with pSIH-H1 shRNA lentivectors (System Biosciences) and packaging mix (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The Lentiviral CRISPR/Cas9 System (System Biosciences LLC, Palo Alto, CA, USA) was then used according to the manufacturer’s protocol to construct the vector ...
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bioRxiv - Cell Biology 2023Quote: ... The 30 mL of lentiviral supernatant was pooled and concentrated to 1/100th of its original volume (300 µL) in DPBS using PEG-iT (System Biosciences), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells (System Biosciences) and mouse embryonic fibroblasts (MEFs ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were co-transfected with 1 µg ePiggyBac transposase expression plasmid (System Biosciences) and 1 µg each epB-MCP-mScarletI ...
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bioRxiv - Cell Biology 2023Quote: Plasmids with expression cassettes flanked by transposable elements were co-transfected with piggyBac transposase (System Biosciences) into clone 5E6L Caco-2BBe cells ...
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bioRxiv - Molecular Biology 2023Quote: ... PB-EF1-MCS-IRES-NEO (System Biosciences, Mountain View, CA) was used as a transposon and its inverted terminal repeats-deleted version was used as a negative control transposon in combination with either pRecLV103-GFP ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293TN cells (System Biosciences, #LV900A-1) were routinely cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Cell Biology 2023Quote: ... from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences). Primer sequences to amplify the 5′- and 3′-side genomic fragments for homologous recombination are as follows ...
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bioRxiv - Cell Biology 2023Quote: The CRISPR guide RNAs were cloned into the CMV-hspCas9-H1-gRNA SmartNuclease vector (System Biosciences, Palo Alto, CA, USA). The Cas9-target sequences for PPARγ-binding sites are as follows ...
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bioRxiv - Cancer Biology 2023Quote: 2.5×106 IFU/ml of pooled lenti-miRNA lentivirus library (System Biosciences Cat. #PMIRHPLVHT-1) was mixed with 4 mg/mL polybrene (Sigma ...
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bioRxiv - Cell Biology 2023Quote: The let-7 overexpression plasmids are commercially available from System Biosciences (SBI). The catalog numbers of the plasmids used in this research are PMIRH000PA-1 (empty vector) ...
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bioRxiv - Genomics 2023Quote: ... A plasmid harboring a synthetic HygR-P2A-2xHA-FKBP_F36V insert was generated with Cold Fusion (System Biosciences), starting with the HygR-P2A-AID cassete in pMGS58 (Ad-dgene #135311 ...
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bioRxiv - Genomics 2023Quote: ... The lentivirus-containing media was mixed at a 1:4 ratio of PEG-it virus precipitation solution (System Biosciences, Cat. No. LV810A-1) to media ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid vector pCDH-EF1-MCS-T2A-copGFP was purchased from System Biosciences. The lentiviral vector was packaged in HEK293T cells as previously described ...
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bioRxiv - Cancer Biology 2023Quote: Mouse SIX4 cDNA (Genomics-online) was cloned into pCDH-CMV-MCS-EF1α-Puro Lentivector (System Biosciences). Human SIX4 cDNA was amplified from genomic DNA by PCR and cloned into pCDH-CMV-MCS-EF1α-Puro vector.
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bioRxiv - Cancer Biology 2023Quote: ... Viral particles were harvested 2 days later and concentrated with Peg-itTM (System Biosciences, Palo Alto, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: The viral vector encoding the anti-CD117 CAR and the RQR8 sequences previously cloned in the pCDH-EF1α-MCS-T2A-GFP lentiviral plasmid (System Biosciences, Palo Alto, CA, USA) as produced as previously reported 14 ...
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bioRxiv - Cancer Biology 2023Quote: ... Luc-plasmid was packaged with pPACKH1 plasmid mix using the protocol described by the manufacturer (System Biosciences Inc.). Cell lines were transduced with Luc-plasmid using Sigma® MISSION® ExpressMag® Beads (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... plasmid pCDH-CMV-MCS2-EF1-Hygro (System Biosciences Inc., Palo Alto, CA, USA) cloned with Luc2 open reading frame (ORF ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 ng μL-1 Cas9 mRNA (System Biosciences, Palo Alto, CA) in 10 mM Tris-HCl pH 8.0 and 0.1 mM EDTA were used for injection into the pronucleus of fertilized mouse zygotes ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was concentrated with PEG-it solution (System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... 70% confluent HEK293TN (System Biosciences, Palo Alto, CA) cells were triple transfected (TransIT-Lenti ...
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bioRxiv - Biochemistry 2023Quote: ... the ERIGIC-53 gene with a C-terminal PA tag was inserted into a PiggyBac Cumate Switch Inducible vector (System Bioscience). The pcDNA-SP-PA-ERGIC-53 plasmid was generated using the In-Fusion cloning kit (Taraka ...
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bioRxiv - Molecular Biology 2023Quote: ... dscGFP-2A-Luciferase gene cassette was amplified from pGreenFire1-ISRE (EF1α-puro) vector (System Biosciences, TR016PA-P) using PCR and cloned in the pEGFP-N1 vector (Clontech ...
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bioRxiv - Molecular Biology 2023Quote: ... in which A375 Cas9 cells were co-transfected with the rescue plasmids as well as the Super PiggyBac Transposase Expression Vector (System Bioscience Inc, PB200PA) at a 4:1 ratio utilising Lipofectamine™ LTX reagents (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The corresponding oligos were ordered from Twist BioScience and cloned into an untagged (System BioSciences, PB533A-2), GFP-tagged (pPB_EF1_MCS-EGFP_IRES_Puro ...
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bioRxiv - Neuroscience 2023Quote: CRTC1 (WT or non-nitrosylatable mutant CRTC1C216A) was subcloned into the lentiviral pCDH-EF1-dTomato-T2A-Kpn21 vector (System Biosciences) and used to generate active lentiviral particles for injection into animals59 ...
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bioRxiv - Neuroscience 2023Quote: ... and concentrated using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s directions ...
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bioRxiv - Physiology 2023Quote: ... ExoQuick (Cat #EXOQ5A-1, System Biosciences, Palo Alto, CA) was added to the supernatant at a 1:4 ratio for 30 min at 4°C ...
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bioRxiv - Physiology 2023Quote: ... and 5 µL of de-fibrination reagent (Cat #TMEXO-1, System Biosciences, Palo Alto, CA) was added to the plasma to remove clotting factors prior to the isolation of EVs ...
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bioRxiv - Physiology 2023Quote: Isolated Evs were processed for total RNA isolation using the SeraMir Exosome RNA purification kit (Cat #RA808A-1, System Biosciences, Palo Alto, CA), according to the manufacturer’s instructions provided with the kit ...
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Immunology 2023Quote: ... The virus was either used directly or concentrated using PEG-it (System Biosciences) before use ...
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...