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Citations for System Biosciences :
501 - 550 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... was constructed by inserting human HAUSP-WT or HAUSP-CS ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences, CD510B-1). The lentiviral plasmid expressing SOX2 was constructed by cloning human SOX2 ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... The lentiviral plasmid expressing SOX2 was constructed by cloning human SOX2 ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences, CD510B-1) or PCW57.1 vector (Addgene ...
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bioRxiv - Neuroscience 2021Quote: ... The number of CD63+ EVs collected in the EV-enriched fractions were estimated by ELISA (System Biosciences). The ELISA standards provided in the kit are calibrated by NTA to measure the number of exosomes and establish a standard curve based on exosome abundance ...
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bioRxiv - Neuroscience 2021Quote: ... the total number of EVs and their size-distribution was quantified by NTA (System Biosciences).
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bioRxiv - Neuroscience 2021Quote: ... and transduced the next day with a lentivirus expressing CD63-GFP (produced from pCT-CD63-GFP, System Biosciences, Palo Alto, CA). The astrocytes expressed GFP between 48 and 72 h after transduction ...
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bioRxiv - Cancer Biology 2021Quote: ... transferred onto nitrocellulose membranes and blotted with the following antibodies: CD9 (System Bioscience) and GM130 (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... Next day they were transfected with HyPB7 and PiggyBac expression plasmids (System biosciences) using lipofectamine 3000 (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: For cDNA expression of WIPI2B proteins cells were transduced with lentivirus carrying the gene of interest with an N-terminal 3X Ty1 tag (Table S7) on the pCDH-CMV-MCS-T2A-Puro backbone (CD522A-1, System Biosciences). cDNA expression of ATG proteins has been previously described (21 ...
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bioRxiv - Developmental Biology 2021Quote: ... The sCMV promoter was amplified from pCS2+ 69 by PCR and cloned into the pCDH-EF1α-MCS-(PGK-GFP-T2A-PURO) bidirectional promoter lentiviral vector (System Biosciences), replacing the PGK promoter ...
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bioRxiv - Cancer Biology 2021Quote: ... The NF-kB-GFP-Reporter was purchased from System Biosciences. The PTPN11T253M/Q257L (TM/QL ...
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bioRxiv - Cancer Biology 2021Quote: ... CD22 and CD133-specific competent CARs and anergic CARs (scFvs) that lacks co-stimulatory and ζ-chain signaling domains were inserted into the lentiviral vector pCDH-T2A-copGFP (System Biosciences). The CD19– mRuby2 fusion was generated by fusing the mRuby2 sequence at the C terminus of CD19 and cloned into the pCDH lentiviral vector ...
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bioRxiv - Cell Biology 2021Quote: Inducible Cas9 iPSCs were generated using an AAVS1 TALE-Nuclease KIT (GE60xA-1; System Biosciences) and an iCas9 HR donor plasmid containing a TRE3G promoter-driven spCas9 with a C-terminal MYC NLS followed by a CMV promoter-driven Tet-On-3G and a neomycin resistance adapted from Ihry et al ...
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bioRxiv - Microbiology 2021Quote: ... isolate USA-WA1/2020) was propagated in Vero cells and concentrated with a polyethylene glycol (PEG) (Cat# LV-810A, System Biosciences, Palo Alto, CA 94303, USA) to a titer of ~1×107 plaque forming units (PFU)/ml ...
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bioRxiv - Immunology 2021Quote: ... and concentrated using 5x PEG-it viral precipitation solution (System Biosciences; LV825A-1). Transduction of B cells was performed 12-24 h after stimulation via spinfection at 800 g for 1 h.
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bioRxiv - Neuroscience 2021Quote: ... Exosomes were extracted from supernatant with an ExoQuick kit (System Biosciences). The presence of exosomal markers and size distribution of the particles was confirmed previously [22] ...
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bioRxiv - Molecular Biology 2021Quote: Exosomes were immunopurified using magnetic beads (Exo-Flow, System Bioscience, Palo Alto, USA) according to the manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and assembled into pCDH-EF1α-MCS-IRES-Puro vector (System Biosciences).
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bioRxiv - Neuroscience 2021Quote: ... and mixed with 1x PEG solution (SBI System Bioscience, CA, USA) to pellet the virus ...
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bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with 10% FBS depleted of exosomes (System Biosciences, EXO-FBS-250A-1). Cells were maintained in this medium for 24 h before supernatant collection for EV purification ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles were harvested either by ultracentrifugation at 106,559 g for 2 h at 4°C using a Beckman SW 32 Ti rotor or using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were cultured in EV-depleted FBS (System Biosciences, Palo Alto, CA). Plates (14 ...
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bioRxiv - Cell Biology 2021Quote: ... the cyclin D1 was generated by PCR insertion into the XPack CMV constructs (System Biosciences). Hsc70D10N was generated by PCR-based site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... and a lentiviral transfer vector CD512-EF1a-RFP (System Biosciences CD512B-1) using Lipofectamine 2000 ...
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bioRxiv - Biochemistry 2021Quote: ... the Su9-DAO construct was cloned into the cumate-inducible PB-CuO-MCS-IRES-GFP-EF1-CymR-Purovector (System Biosciences; PiggyBac system). Positive clones were selected with DMEM complete containing 2μg/ml puromycin and expression of the cell line was induced with 30 μg/ml cumate for at least 16h before the experiment.
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bioRxiv - Neuroscience 2021Quote: ... The resulting PCR fragment was inserted by means of the cold fusion reaction (System Biosciences; CA, USA) into the EcoRI-XbaI-digested pCaSpeR-mb247-hs-GCamP3 vector (gift from T ...
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bioRxiv - Biochemistry 2021Quote: ... the entire biosensor cassette was subcloned to PiggyBac Transposon vector system (SBI System Biosciences) by In-Fusion HD cloning kit ...
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bioRxiv - Biochemistry 2021Quote: Samples of plasma or CSF were mixed with ExoQuick Exosome Precipitation Solution (System Biosciences) or ExoQuick ULTRA EV Isolation Kit for Serum and Plasma (System Biosciences) ...
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bioRxiv - Biochemistry 2021Quote: ... or ExoQuick ULTRA EV Isolation Kit for Serum and Plasma (System Biosciences), and protocols were performed according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience). Sequences of all plasmids were confirmed by Sanger sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... the envelope plasmid (pMD2.G) and the packaging plasmid (pCMV-deltaR8.91) (System Biosciences, CA, USA) into HEK293T cells according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The SETD7 cDNA was cloned into the EcoRI/BamHI site of a pCDH-CMV-MCS-EF1α -Puro vector (System Biosciences, Palo Alto, CA, USA). The sequences were confirmed by Sanger sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... pCDH-CMV-MCS-EF1-Puro (System Biosciences) for the viral infection and expression of N-terminal FLAG-tagged USP8 ...
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bioRxiv - Cell Biology 2021Quote: Plasmids expressing full length mouse ATF6α and ATF6β were constructed by inserting ATF6α and ATF6β cDNAs into the pCDF1-MCS2-EF1-Puro expression vector (System Biosciences, Palo Alto, CA, USA). pcDNA3.1(+ ...
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bioRxiv - Immunology 2021Quote: ... A PEG-it Virus Precipitation Solution (Cat# LV810A-1, System Biosciences, Palo Alto, CA 94303, USA) was added to 40ml of virus culture at a 1:4 ratio ...
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bioRxiv - Biochemistry 2021Quote: The NF-κB/Jurkat/GFPTM Transcriptional Reporter cell line was obtained from System Biosciences, and was cultured in RPMI-1640 media supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... following manufacturer instructions (QuantiMir, System Biosciences, cat. RA670A-1). To acquire the miRNA-enriched RNA fraction from adipose tissue miRNeasy Mini Kit was used (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... The transfer vector was then packaged into a lentivirus using the pPACKH1 HIV third generation lentiviral expression system kit and PureFection reagent (both from System Biosciences, Palo Alto, CA) into HEK293-T cells (ATCC ...
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bioRxiv - Molecular Biology 2021Quote: ... This vector was modified from the original vector (PB-CuO-MCS-IRES-GFP-EF1α-CymR-Puro (System Biosciences)) by replacing the IRES-GFP cassette with a BGH polyA signal ...
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bioRxiv - Immunology 2021Quote: 293TN Producer cell line (System Biosciences, Cat #LV900A-1) was maintained in DMEM containing 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... Construct expressing lipin-1-mCherry was amplified by PCR adding 5’Xba-I and Sal-I 3’specific restriction sites and inserted into lentiviral vector pCDH-CMV-MCS-EF1α-copGFP (System Biosciences) after removing an internal Sal-I restriction site by mutagenesis ...
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bioRxiv - Immunology 2021Quote: ... pCDH-CMV-MCS-EF1α-RFP (System Biosciences). To facilitate the secretion and purification of the protein ...
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bioRxiv - Immunology 2021Quote: ... HEK-293TN (System Bioscience) cells were plated in 15-cm dish complete DMEM medium ...
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bioRxiv - Immunology 2021Quote: IL-23 EEV(EEV651A-1, System Biosciences) was hydrodynamically injected in a volume of sterile HBSS (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Spike-expressing pseudovirus was generated with System Bioscience’s pPACK-SPIKE packaging system (System Biosciences, Cat # CVD19-50A-1) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... 5x PEG-it Virus Precipitation Solution (System Biosciences, Cat # LV810A-1) was added to supernatants and incubated 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... 8×106 293TN Producer cells (System Biosciences, Cat # LV900A-1) were plated in T150 flasks overnight ...
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bioRxiv - Immunology 2021Quote: ... 55 mL of PureFection reagent (System Biosciences; Cat # LV750A-1) was added to each 1mL tube ...
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bioRxiv - Molecular Biology 2021Quote: ... This ORF was then subcloned into a pCDH-puro lentiviral vector (System Biosciences) via NheI and XhoI sites or amplified and cloned via EcoRI and AgeI sites into the pLVX-puro lentiviral vector (System Biosciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... via NheI and XhoI sites or amplified and cloned via EcoRI and AgeI sites into the pLVX-puro lentiviral vector (System Biosciences). The N40A mutation was generated by standard site-directed mutagenesis and all vectors were confirmed by Sanger sequencing of the inserts ...
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bioRxiv - Molecular Biology 2021Quote: ... a lentiviral Lenti/anti-miR-31 (antimiR-31) was created using commercial plasmids pLenti/anti-miR-31 (System Biosciences, Palo Alto, CA). A Lenti/anti-miR-Cont (antimiR-Cont ...