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Citations for System Biosciences :
651 - 677 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... purification of EVs was performed in plasma by UC-I (bellow) and in conditioned medium according to one of the following protocols (Fig.1a):-(Exoquick) ExoQuick® commercial kit (System Biosciences EXOTC10A-1, Palo Alto, CA), following manufacturer’s instructions;
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then collected and incubated with PEG-it™ (System Biosciences) in a 5X solution at 4C for 2 days to precipitate the viruses ...
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bioRxiv - Molecular Biology 2020Quote: ... These constructs were prepared in a pCDH-EF1α-MCS-BGH-PGK-GFP-T2A-Puro vector (System Biosciences). To monitor the expression of every possible RAN protein ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid was then packaged into viral particles using the pPACKH1 lentivector Packaging Kit (System Bioscience) in HEK293T cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Viral particles were precipitated from media using PEG-it (System Biosciences, LV810A-1). CP-A-empty and CP-A-ERBB2 stable cells were generated by transfecting 1×106 CP-A cells with lentiviral particles containing either pHAGE-ERBB2 or pHAGE-empty using polybrene (EMD Millipore ...
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bioRxiv - Cell Biology 2020Quote: Lentivirus was generated with PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA no LV810A-1) using a LV H2B-GFP plasmid (Addgene no 25999 ...
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bioRxiv - Cancer Biology 2020Quote: SW480 CRC cells were transduced with lentivirus carrying pCDH vector from System Biosciences: empty vector (Mock ...
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bioRxiv - Genomics 2020Quote: ... multiple viral supernatants were collected and concentrated using PEG-it Virus Precipitation Solution (System Biosciences LV810A-1). Cells were infected 2-3 times at 12-hour intervals before selection in the appropriate antibiotic.
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bioRxiv - Neuroscience 2020Quote: ... fibrinogen and fibrin proteins known to impede efficient EV recovery were removed using 5 U/mL of thrombin (System Biosciences, Inc.; Mountainview, CA) for 30 minutes at RT followed by addition of 495 μL of Dulbecco’s PBS-1X (DPBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Supernatants were collected and concentrated by PEG-it (System Biosciences).
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bioRxiv - Cell Biology 2020Quote: Lentiviral cloning and expression vectors pCDH-UbC-MCS-EF1-Hygro (CD615B-1) and pCDH-EF1-MCS-T2A-Puro (CD520A-1) were purchased from System Biosciences Inc ...
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bioRxiv - Cell Biology 2020Quote: ... was transferred into pCDH-CMV-MCS-EF1-Puro (System Biosciences, cat #CD510B-1) using NheI/NotI sites for virus production.
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bioRxiv - Cell Biology 2020Quote: ... We PCR amplified the EC-BioID sequence and inserted it into the PiggyBac-GFP vector using NheI site (PB533A, System Biosciences, Mountain View, CA). For C-BioID ...
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bioRxiv - Cell Biology 2020Quote: ... into the third-generation lentiviral plasmid pCDH-EF1-IRES-Puro (System Biosciences).
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bioRxiv - Evolutionary Biology 2020Quote: ... The Cold Fusion system (System Biosciences) was used to insert these into the vector via recombination ...
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bioRxiv - Genetics 2020Quote: ... The ZYCY10P32T E.Coli strain and the minicircle backbone were purchased from System Bioscience. After cloning in the sequence into the specific minicircle backbone the plasmid is transformed into the ZYCY strain ...
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bioRxiv - Biophysics 2020Quote: ... using NheI and NotI and subcloning into pCDH-puro (System Biosciences). This resulting plasmid has been deposited with Addgene (plasmid #82724).
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bioRxiv - Bioengineering 2020Quote: ... these HEKs were genetically-modified reporter cells (NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line; System Biosciences, catalogue number TR860A-I), but reporter activity is not relevant here.
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bioRxiv - Immunology 2020Quote: ... All other 293T-GFP responder cell lines were constructed by lentiviral transduction (pCDH vector backbone, System Biosciences). All transient expression plasmids were cloned into the pCS2+ vector using standard restriction cloning ...
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bioRxiv - Molecular Biology 2020Quote: An amount of 4 ng of XMIRXpress vector containing Non-Targeting miRNA with Xmotif (cat. no. XMIRXP-NT, System Biosciences LLC, Palo Alto, CA, USA) was introduced into 100 μL Stellar competent E ...
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bioRxiv - Molecular Biology 2020Quote: The Jurkat cell transductions were done with multiple viral titers using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... filtered and concentrated using PEG-it Virus Precipitation Solution (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The WPRE 3’-UTR sequence was amplified from the CD813A-1 (System Biosciences) vector.
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bioRxiv - Immunology 2020Quote: To silence miR-424 the GFP tagged lentiviral vectors that express antisense hsa-miR-424 (MZIP424-PA-1) and corresponding scramble control lentiviral vectors (MZIP000-PA-1) were purchased from SBI (System Biosciences). Transfection of 293T packaging cell line was performed using calcium-phosphate protocol and infection of target cells was done as previously described (Platzer et al. ...
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bioRxiv - Immunology 2020Quote: ... These constructs were cloned into the episomal vector EEV600A-1 (System Biosciences) into which a puromycin resistance cassette was added so that cell pools expressing the proteins could be rapidly selected ...
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bioRxiv - Cell Biology 2020Quote: ... collected after 24 hours and concentrated using Peg-It Virus precipitation solution (cat# LV810A-1, System Biosciences) according to the manufacturer’s manual.
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bioRxiv - Neuroscience 2020Quote: ... All plasmids were subcloned into a pCDH expression vector (System Biosciences), assembled using Gibson Assembly (New England Biolabs ...