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Citations for Mirus Bio :
151 - 200 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... or TransIT-X2 Transfection Reagent (Mirus) as per the manufacturers’ instructions.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... using a 3:1 ratio of TransIT-2020 (Mirus Bio) µL:µg total DNA ...
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bioRxiv - Neuroscience 2024Quote: ... 10 μg of the transfer vector of interest and three volumes of TransIT-LT1 Transfection Reagent (Mirus). 48 h after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... virus was produced using Lenti-X 293T cells in 24-well plates using TransIT LT1 (Mirus Bio LLC #MIR 2305) and second-generation packaging vectors pMD2.G and psPAX2 according to standard lentiviral production protocols.
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bioRxiv - Biophysics 2024Quote: ... using TransIT®-293 Transfection Reagent (Mirus Bio LLC #MIR2700) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The resultant plasmid containing the fusion gene of RBK21 and PILRa was introduced into PLAT-E cells using TransIT-293 (Mirus) and recombinant retrovirus were harvested from supernatant 3 days after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... plasmids encoding Drg proteins on strong CMV promoters using the Transit293 transfection system (Mirus Bio) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... VERO and HEK293-Flp-In™-T-REx™ were produced by seeding 100,000 cells in a 24-well plate and transfecting 800 ng of the corresponding plasmids using TransIT®-LT1 (Mirus Bio). The medium was changed after 24 h incubation.
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bioRxiv - Neuroscience 2024Quote: ... 1.25 µg of pMD2.G and 0.625 µg of psPAX2 were inoculated to each well using Mirus transIT-X2 transfection reagent (Mirus). Culture medium was changed to fresh medium at 4∼24 hr post transfection ...
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bioRxiv - Neuroscience 2024Quote: ... by using transfection reagent TransIT X2 (Mirus, Cat. no. MIR6000) and following standard protocol posted on the product page of TransIT X2 ...
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bioRxiv - Microbiology 2024Quote: ... into HEK293T cells using TransIT-LT1 (Mirus). Then ...
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bioRxiv - Genetics 2024Quote: ... lentiviral particles were produced by transfecting standard packaging vectors into HEK293T using TransIT-LT1 Transfection Reagent (Mirus, MIR2306). At <24 hours post-transfection culture media with exchanged with fresh media supplemented with ViralBoost (Alstem Bio ...
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bioRxiv - Evolutionary Biology 2024Quote: ... HEK-293T cells were co-transfected using TransIT-Lenti transfection reagent (Mirus, USA), with 500ng psPAX2 (addgene ...
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bioRxiv - Plant Biology 2024Quote: ... The LabelX reagent was prepared by firstly resuspending Label-IT Amine Modifying Reagent (Mirus Bio, LLC) was resuspended in the provided Reconstitution Solution at 1 mg/mL ...
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bioRxiv - Genomics 2024Quote: ... and after 24 hours transfected with 0.5 µ g variant-encoding plasmid using TransIT transfection reagent (Mirus Bio, #MIR6606). 24 hours after transfection ...
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bioRxiv - Systems Biology 2024Quote: ... and packaging plasmids psPAX2 and pMD2G using TransIT-LTI Transfection Reagent (Mirus, MIR 2300). 24 hr post-transfection ...
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bioRxiv - Systems Biology 2024Quote: Lentivirus was produced by co-transfecting LentiX™ 293T cells with transfer plasmids and standard packaging vectors (psPAX2, pMD2G) using TransIT-LTI Transfection Reagent (Mirus, MIR 2300). When required ...
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bioRxiv - Microbiology 2024Quote: ... in serum-free DMEM and the TransIT-LT1 reagent (Mirus Bio, MIR2305). Virus was harvested and J-Lat cells were transduced with the TxLatent library at a multiplicity of infection of 0.2 ...
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The intersection of endocrine signaling and neuroimmune communication regulates neonatal nociceptionbioRxiv - Neuroscience 2024Quote: ... Cells were transfected using 10 nM of siRNAs with 2.5 µl TransIT-TKO® Transfection reagent (Mirus) for 24 hrs following manufacturer’s recommended protocols ...
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bioRxiv - Microbiology 2024Quote: Chimeric receptors were transiently expressed in HEK Slc35A1 KO cells by transfection of plasmids with Transit-X2 dynamic delivery system (Mirus Bio) 24hrs prior to virus (Ca’09 ...
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bioRxiv - Microbiology 2024Quote: Plasmids were transfected into HEK293T cells using the TransIT 293 transfection reagent (Mirus). Wells were transfected with 1ug plasmid ...
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bioRxiv - Microbiology 2024Quote: ... All transfections were carried out using X2 polymer (Mirus) using 1μg of plasmid where indicated or 5μg ...
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bioRxiv - Microbiology 2024Quote: ... PCR products wildtype and relaxed were then transfected into T7 expressing Baby Hamster Kidney Cells (33) (BHK: BSRT7) using the X2 transfection system according to manufacturer specifications (Mirus, Madison, WI). Transfections were incubated overnight ...
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bioRxiv - Microbiology 2024Quote: ... into Lenti-293T cells using a TransIT-Lenti Transfection Reagent (Mirus Bio, # MIR 6604). Supernatants were collected 48 hours post transfection and lentivirus were detected by a Lenti-X™ GoStix™ Plusx kit (TaKaRa ...
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bioRxiv - Microbiology 2024Quote: ... and 2 µg/mL puromycin (Mirus). African green monkey Vero [ATCC CCL-81] and N2a ΔB4galt7ΔLDLR-TC LDLR (N2a ΔB4galt7-LDLR ...
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bioRxiv - Microbiology 2024Quote: ... or mock-transfected using TransIT-mRNA Transfection Kit (Mirus Bio) and incubated for 2 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: Plasmids were transfected in HEK293T using Trans-IT X2 (Mirus Bio). The following day ...
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bioRxiv - Neuroscience 2024Quote: Lentiviral particles were produced and quantified in HEK293T cells with Mirus TransIT-293 Transfection Reagent (Mirus Bio LLC, Madison, WI, USA) as described before (20) ...
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bioRxiv - Microbiology 2024Quote: ... 1.25µg of psPAX2 and 250ng of VSVG and 8uL LT-1 transfection reagent (Mirus). Media was changed 24 h post transfection to DMEM + 10% FCS + 1g/100mL Bovine Serum Albumin (BSA) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected according to the manufacturer’s instructions using TransIT-LT2020 (Mirus) at the ratio of 3 µL TransIT-LT2020 to 1 µg DNA ...
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bioRxiv - Microbiology 2024Quote: ... and 200ng pMD-VSVG(82) using Transit-LT1 (Mirus Bio), changing media after 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... and topped up to 2.2µg total plasmid DNA mass with pBlueScript using TransIT-LT1 (Mirus Bio). At 24 hours post- transfection ...
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bioRxiv - Microbiology 2024Quote: ... Scramble control siRNAs or specific siRNAs against indicated host targets were transfected at 50 nM for 2 days using the TransIT-X2 dynamic delivery system (Mirus, #MIR6000). At day 2 post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... using TransIT®-293 Transfection Reagent (Mirus, MIR 2700). 48 hr post-transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... the IVOC product containing all the genomic plasmids for virus particle synthesis was fully transfected into HEK293T cells (1×106 cells) with the use of TransIT-293 Transfection Reagent (Mirus). 48 hours after transfection ...
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Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brainbioRxiv - Neuroscience 2024Quote: ... 1% L-Glutamic Acid and 1% Penicillin-Streptomycin at 37°C in 5% CO2 and transfected with plasmids (Mirus TransIT-LT1 Transfection Reagent). Imaging was performed 24hr following transfection in external Tyrode solution (119mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... using CHOgro high yield expression system (Mirus Bio, Madison, WI, USA) according to the manufacturer’s protocol and purified following the protocol by Nesspor et al ...
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bioRxiv - Neuroscience 2024Quote: ... Primary cortical neurons were electroporated using Ingenio electroporation solution (Mirus) following the manufacturer’s recommendations and the proportion 2 μg DNA:2*106 cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected using TransIT-Lenti (Mirus) for 24 h ...
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bioRxiv - Microbiology 2024Quote: ... in HEK293 cells using TransIT-LT1 (Mirus). After 48 h ...
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bioRxiv - Cell Biology 2024Quote: ... and 1.5 µg of a pLJM1 or pLIX403 plasmid using the TransIT-LT1 Transfection Reagent (Mirus Bio) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... All transfections were performed via lipofection using TransIt-LT1 transfection reagent (Mirus). Astrocyte progenitors were passaged into 24 well plates one day before the transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... XLone-derived constructs were co-transfected with a plasmid encoding piggybac transposase into MCF7 cells with TransIT-LT1 Transfection Reagent (Mirus). Transfected cells were selected for blasticidin resistance for 2 weeks ...
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bioRxiv - Molecular Biology 2024Quote: ... hsPB126 was transfected with Cas9 landing pad plasmid (pHPHS800) and Bxb1 expression plasmid (pHPHS115) using TransIT-LT1 reagent (Mirus). 72 hours post-transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... On the day of transfection 0.5 µg total DNA is diluted in 50 µL of OptiMEM per well and 1.5 µL TransIT-X2 transfection reagent (Mirus, MIR 6003). Further details about transfection conditions are provided in the Supplementary Files.
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bioRxiv - Microbiology 2024Quote: ... into BSR-T7 cells using the LT-1 transfection reagent (Mirus), and passaged to generate a P1 stock as above ...
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bioRxiv - Immunology 2024Quote: ... and used to produce infectious baculovirus according to the manufacturer’s instructions using the FlashBac Ultra system (Mirus Biotech) and transfection into Sf21 cells (Clontech).
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bioRxiv - Immunology 2024Quote: ... The sequence was subcloned into a baculovirus shuttle vector (Mirus Biotech) and used to produce infectious baculovirus according to the manufacturer’s instructions using the FlashBac Ultra system (Mirus Biotech ...
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bioRxiv - Synthetic Biology 2024Quote: ... the Mirus Ingenio electroporation solution (Mirus Bio: MIR 50114) was pre-warmed by shaking at 37°C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The transfection mix was prepared by mixing 2 ml Opti-MEM medium with 2.5 µg of each of the 8 RG plasmids and 60 µl TransIT® -LT1 transfection reagent (Mirus Bio) and incubation at room temperature (RT ...