Labshake search
Citations for Mirus Bio :
351 - 400 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: The donor template and Cas9/gRNA plasmids were reverse transfected into H9 hESCs using TransIT-LT1 (Mirus) according to manufacturer recommendations ...
-
bioRxiv - Developmental Biology 2024Quote: ... Overexpression constructs were introduced by co-transfection with Flp recombinase using LT1 transfection reagent (Mirus). Selected KH2-SETα and KH2-SETβ clones were expanded and maintained on low hygromycin (100 µg/mL) ...
-
bioRxiv - Developmental Biology 2024Quote: ... using lipofection with TransIT-293 (Mirus Bio 2700). Cells were seeded at clonal density and selection in G418 (250 mg/ml ...
-
bioRxiv - Developmental Biology 2024Quote: ... Parental KH2 mESCs were co-transfected with the targeting vector and a pX330-neoR vector expressing Cas9 and gRNAs targeting Ehmt2 using TransIT-293 (Mirus Bio 2700). The next day ...
-
bioRxiv - Cell Biology 2024Quote: ... and 1.2 μg cytomegalovirus 8.91 vector were prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio; Madison, WI). Three days post transduction virus-containing supernatants were harvested and concentrated approximately 40-fold using Lenti-X Concentrator (Clontech ...
-
bioRxiv - Genetics 2024Quote: ... and TransIT®-LT1 Transfection Reagent (Mirus Bio), following the manufacturer’s protocol ...
-
bioRxiv - Genetics 2024Quote: ... and TransIT®-LT1 Transfection Reagent (Mirus Bio), following the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2024Quote: ... HEK293T cells in 6-well plates were transiently transfected with 1 µg of PSMB-3xFLAG constructs using TransIT-293 transfection reagent (Mirus). To analyze interactions between KCMF1-3xFLAG mutants and endogenous proteins ...
-
bioRxiv - Cell Biology 2024Quote: ... Plasmid transfections were carried out using TransIT-293 transfection reagent (Mirus) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Lentiviral particles were generated by co-transfection of HEK 293T packaging cells with a lentiviral expression construct containing dCas9-KRAB and packaging constructs pMD VSV-G and pCMV Δ8.91 at a ratio of 1:0.7:0.3 using TransIT-293 transfection reagent (Mirus Bio, 2704). Media containing the lentivirus were collected 48 h post-transfection ...
-
bioRxiv - Cell Biology 2024Quote: ... packaging and envelope plasmids using TransIT-293 transfection reagent (Mirus Bio, 2704), in 1:0.7:0.3 ratio.
-
bioRxiv - Cell Biology 2024Quote: ... HEK- 293T cells were co-transfected with a lentiviral expression construct and the packaging vectors pCMVΔ8.91 and pMD VSV-G at a ratio of 1:0.7:0.3 using TransIT-293 (Mirus Bio) as per the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: ... Transfection was performed using the TransIT-LT1 transfection reagent (Mirus) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2024Quote: ... were co-transfected with the pCMV-dR8.91 packaging and pMD2.G envelope plasmids into the packaging HEK293T cells (AVAM761) using the TransIT-293 Transfection Reagent (Mirus Bio). Following daily media changes (DMEM ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... or LabelIT DNP Labeling kit (Mirus) to generate respective DIG-labeled or DNP-labeled probes ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 100 ng BestBac 2.0 Δ v-cath/chiA Linearized Baculovirus DNA (Chimigen) and 1,2 µl TransIT (Mirus Bio) to produce final recombinant baculovirus ...
-
bioRxiv - Developmental Biology 2024Quote: ... Transfection was performed using TransIT-Insect Transfection Reagent (Mirus Bio, Cat# MIR6104), following the protocol provided but with a reduced amount of each DNA construct (50-200 ng ...
-
bioRxiv - Cell Biology 2024Quote: ... was mixed with 1.5 μl X2 Transfection Reagent (Mirus) and 200ng plasmid DNA (pEGFP-C2 with or without inserts derived from CIZ1) ...
-
bioRxiv - Cell Biology 2024Quote: ... with TransIT-Lenti reagent (Mirus Bio) and subsequently added to 70% confluent HEK293 cells followed by 48h incubation at 37°C and 5% CO2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with the STING-GFP expression constructs using the Trans-IT transfection reagent (Mirus). After transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... using Trans-IT transfection reagent (Mirus) 24 hours after seeding ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were transiently transfected with both of the constructs using Trans-IT transfection reagent (Mirus) according to the manufacturer’s recommended protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... TransIT-LT1 transfection reagent (Mirus) was used according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... using Trans-IT transfection reagent (Mirus) according to the manufacturers ‘ protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... and 20.5 µg pcDNA3.1 VSV-G in 4.9 ml DMEM with 345 µl TransIT-Lenti Transfection Reagent (Mirus Bio, MIR 6600) added to 4.8 ml DMEM ...
-
bioRxiv - Neuroscience 2023Quote: ... and 5 µg of the transfer plasmid (pLV-TetO-hNGN2-eGFP-puro, FudeltaGW-rtTA, myc-tagged SYNJ2BP WT, S21A or S21E) using TransIT-Lenti reagent (Mirus Bio). After 48 h (day 2) ...
-
Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... cells were transfected with0.5 μg of NS4B-encoding plasmids using TransIT-LT1 Transfection Reagent (Mirus) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... whereas MEF cells used TransIT-X (Mirus) following manufacturer protocol with varied DNA concentrations ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells we resuspended in Ingenio® Electroporation Solution (Mirus Bio #MIR50114) such that the cell concentration was at 8 × 106 per electroporation in 800μL final volume ...
-
bioRxiv - Microbiology 2023Quote: ... using TransIT®-LT1 Transfection Reagent (Mirus Bio LLC; Madison, Wisconsin, USA). After 24 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... and gRNA plasmids (100 ng) using 1.875 μL of TransIT-293 (Mirus) transfection reagent in OptiMEM to a final volume of ∼30 μL ...
-
bioRxiv - Systems Biology 2023Quote: The DNA library was co-transfected with pCMV-dR8.91 and pMD2.G plasmids using TransIT-Lenti (Mirus) into HEK293 cells ...
-
bioRxiv - Neuroscience 2023Quote: ... using 25 µL TransIT®-LT1 Transfection Reagent (Mirus Bio, Cat. No. MIR 2300) in a final volume of 1mL DMEM medium supplemented with 10% tetracycline-free FBS ...
-
bioRxiv - Microbiology 2023Quote: ... pCAGGS-HA plasmids were transfected using 1µL TransIT 2020 (Mirus) per 100µg plasmid and incubated overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... with either TransIT-LT1 (Mirus Bio, HeLa cells) or LipofectamineTM 2000 (Thermo Fisher ...
-
bioRxiv - Cell Biology 2023Quote: ... Co-transfection of siRNA was performed with TransIT-X2 (Mirus Bio) and experiments perfomed 48 h later.
-
bioRxiv - Microbiology 2023Quote: ... Total of 2.49 μg plasmid DNA with 6.25 μL TransIT LT1 transfection reagent (Mirus) in 250 μL Opti-MEM (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... Medium was changed 24 h later to 10 ml of 5 % FBS/DMEM and changed again 48 h later to 10 ml of 5 % FBS/DMEM containing 1 µg/ml of puromycin (Mirus). Cells were incubated in selection medium for 72 h ...
-
bioRxiv - Biochemistry 2023Quote: ... TransIT (Mirus, MIR2300), Qubit dsDNA HS Assay Kit (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... The pCS2 NLS-mCherry-PRIMPOL WT or S255D vectors were used for transfection in U2OS PRIMPOL KO cells using TransIT (Mirus) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... Transfection was carried out using TransIT (Mirus) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... and incubated with 18 µl of TransIT-LT1 Reagent (Mirus) for 30 min at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... transfection of plasmid constructs was performed using Trans-IT 2020 reagent (Mirus Bio, #MIR 5400) following the manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 66 µl TransIT-LT1 (Mirus MIR 2306) were combined with 2.1 ml of Opti-MEM to produce TransIT-LT1:DNA complexes ...
-
bioRxiv - Biophysics 2023Quote: ... The larger 100μL nuclecuvette was used and a single 10cm dish of cells was resuspended in 100uL of Ingenio electroporation solution for nucleofection (Mirus; Madison, WI). Approximately 18 h after transfection ...
-
bioRxiv - Cell Biology 2023Quote: ... shRNA lentivirus was prepared by transfecting 293T cells using TransIT-293 (Mirus) using a standard protocol from Addgene.
-
bioRxiv - Neuroscience 2023Quote: ... The striatal cells were then subjected to electroporation (AMAXA nucleofector I: program 05) in a buffer (Mirus Bio) along with plasmid (2ug plasmid per 2 million cells) ...
-
bioRxiv - Biochemistry 2023Quote: Flp-In 293 T-Rex cells were transfected with the different pcDNA5-FRT-TO plasmid constructs and the pOG44 vector using the TransIT2020 cationic lipid (Mirus Bio LLC), according to the manufacturer’s instructions (ThemoFisher Scientific) ...
-
bioRxiv - Biochemistry 2023Quote: ... or Transit-2020 (Mirus Bio LLC ...
-
bioRxiv - Cell Biology 2023Quote: ... Label-IT ® Amine Modifying Reagent (Mirus Bio, LLC) was resuspended in the provided Mirus Reconstitution Solution at 1mg/ml and stored frozen in a desiccated environment ...